Difference between revisions of "Team:UChicago/Collaborations"

Over the summer, our iGEM team started corresponding with the Washington University in St. Louis team. We decided to collaborate with one another by testing both of our projects at the same time.

The WashU team is comparing the “effectiveness of several genes on the level of UV radiation tolerance in E. coli”. They are using four genes that provide UV resistance: uvsE, Dsup, phrAC and BCP. This October, they mailed us these four plasmids so we could put them into our chromosome-integrating plasmids (then transform them into yeast).

If we are able to successfully transform the WashU plasmids, we will be able to demonstrate that our construct has practical applications to fellow researchers. We will also be able to test the UV resistance conferred by these genes by (hopefully) observing the yeast under UV radiation. By reporting our results to the WashU team, they will gain valuable insight into the genes they have chosen to work with.