InterLab
Experimental Values: | Raw Abs600 | Raw Fluorescence | |||||||||||||||||||||||||||
Sample set: | Replicate 1 | Replicate 2 | Replicate 3 | Replicate 4 | Replicate 1 | Replicate 2 | Replicate 3 | Replicate 4 | |||||||||||||||||||||
Blank media | 0.039 | 0.038 | 0.038 | 0.038 | 11.92 | 11.32 | 11.99 | 11.91 | |||||||||||||||||||||
Blank mean: | 0.03825 | 11.785 | OD - Background | Fluorescence - Background | uM Fluorescein / OD600 | Summary Statistics | |||||||||||||||||||||||
Hour 0: | Replicate 1 | Replicate 2 | Replicate 3 | Replicate 4 | Replicate 1 | Replicate 2 | Replicate 3 | Replicate 4 | Replicate 1 | Replicate 2 | Replicate 3 | Replicate 4 | Arith. Mean | Arith. Std.Dev. | Geo. Mean | Geo. Std. Dev. | |||||||||||||
Negative Control (Colony 1) | 0.047 | 0.047 | 0.047 | 0.047 | 12.75 | 12.83 | 13.35 | 12.82 | 0.00875 | 0.00875 | 0.00875 | 0.00875 | 0.965 | 1.045 | 1.565 | 1.035 | 0.10 | 0.10 | 0.16 | 0.10 | 0.12 | 0.03 | 0.11 | 1.246 | |||||
Negative Control (Colony 2) | 0.047 | 0.047 | 0.047 | 0.046 | 10.89 | 9.781 | 7.717 | 12.44 | 0.00875 | 0.00875 | 0.00875 | 0.00775 | -0.895 | -2.004 | -4.068 | 0.655 | -0.09 | -0.20 | -0.41 | 0.07 | -0.16 | 0.20 | #NUM! | #NUM! | |||||
Positive Control (Colony 1) | 0.047 | 0.048 | 0.047 | 0.047 | 12.13 | 12.44 | 13.99 | 13.51 | 0.00875 | 0.00975 | 0.00875 | 0.00875 | 0.345 | 0.655 | 2.205 | 1.725 | 0.03 | 0.06 | 0.22 | 0.17 | 0.12 | 0.09 | 0.09 | 2.410 | |||||
Positive Control (Colony 2) | 0.049 | 0.047 | 0.046 | 0.046 | 13.46 | 12.61 | 13.06 | 13.77 | 0.01075 | 0.00875 | 0.00775 | 0.00775 | 1.675 | 0.825 | 1.275 | 1.985 | 0.14 | 0.08 | 0.14 | 0.22 | 0.15 | 0.06 | 0.14 | 1.505 | |||||
Test Device 1: J23101.BCD2.E0040.B0015 (Colony 1) | 0.049 | 0.05 | 0.048 | 0.049 | 18.19 | 20.69 | 19.15 | 19.37 | 0.01075 | 0.01175 | 0.00975 | 0.01075 | 6.405 | 8.905 | 7.365 | 7.585 | 0.52 | 0.66 | 0.66 | 0.62 | 0.62 | 0.07 | 0.61 | 1.119 | |||||
Test Device 1: J23101.BCD2.E0040.B0015 (Colony 2) | 0.047 | 0.046 | 0.047 | 0.046 | 21.62 | 19.76 | 20.76 | 18.28 | 0.00875 | 0.00775 | 0.00875 | 0.00775 | 9.835 | 7.975 | 8.975 | 6.495 | 0.99 | 0.90 | 0.90 | 0.73 | 0.88 | 0.10 | 0.88 | 1.132 | |||||
Test Device 2: J23106.BCD2.E0040.B0015 (Colony 1) | 0.047 | 0.046 | 0.047 | 0.047 | 13.95 | 14.37 | 14.62 | 14.54 | 0.00875 | 0.00775 | 0.00875 | 0.00875 | 2.165 | 2.585 | 2.835 | 2.755 | 0.22 | 0.29 | 0.28 | 0.28 | 0.27 | 0.03 | 0.27 | 1.147 | |||||
Test Device 2: J23106.BCD2.E0040.B0015 (Colony 2) | 0.048 | 0.047 | 0.046 | 0.047 | 14.38 | 16.27 | 13.38 | 15.53 | 0.00975 | 0.00875 | 0.00775 | 0.00875 | 2.595 | 4.485 | 1.595 | 3.745 | 0.23 | 0.45 | 0.18 | 0.38 | 0.31 | 0.12 | 0.29 | 1.523 | |||||
Test Device 3: J23117.BCD2.E0040.B0015 (Colony 1) | 0.048 | 0.048 | 0.071 | 0.049 | 11.44 | 12.69 | 14.09 | 11.64 | 0.00975 | 0.00975 | 0.03275 | 0.01075 | -0.345 | 0.905 | 2.305 | -0.145 | -0.03 | 0.08 | 0.06 | -0.01 | 0.03 | 0.05 | #NUM! | #NUM! | |||||
Test Device 3: J23117.BCD2.E0040.B0015 (Colony 2) | 0.047 | 0.046 | 0.045 | 0.051 | 13.57 | 13.91 | 12.65 | 13.24 | 0.00875 | 0.00775 | 0.00675 | 0.01275 | 1.785 | 2.125 | 0.865 | 1.455 | 0.18 | 0.24 | 0.11 | 0.10 | 0.16 | 0.06 | 0.15 | 1.504 | |||||
Test Device 4: J23101+I13504 (Colony 1) | 0.045 | 0.046 | 0.047 | 0.047 | 14.09 | 13.1 | 14.81 | 13.72 | 0.00675 | 0.00775 | 0.00875 | 0.00875 | 2.305 | 1.315 | 3.025 | 1.935 | 0.30 | 0.15 | 0.30 | 0.19 | 0.24 | 0.08 | 0.23 | 1.416 | |||||
Test Device 4: J23101+I13504 (Colony 2) | 0.048 | 0.046 | 0.046 | 0.046 | 15.34 | 14.29 | 13.91 | 13.42 | 0.00975 | 0.00775 | 0.00775 | 0.00775 | 3.555 | 2.505 | 2.125 | 1.635 | 0.32 | 0.28 | 0.24 | 0.18 | 0.26 | 0.06 | 0.25 | 1.267 | |||||
Test Device 5: J23106+I13504 (Colony 1) | 0.046 | 0.046 | 0.047 | 0.046 | 13.8 | 11.54 | 12.31 | 10.5 | 0.00775 | 0.00775 | 0.00875 | 0.00775 | 2.015 | -0.245 | 0.525 | -1.285 | 0.23 | -0.03 | 0.05 | -0.15 | 0.03 | 0.16 | #NUM! | #NUM! | |||||
Test Device 5: J23106+I13504 (Colony 2) | 0.047 | 0.047 | 0.046 | 0.046 | 13.06 | 14.03 | 13.71 | 13.77 | 0.00875 | 0.00875 | 0.00775 | 0.00775 | 1.275 | 2.245 | 1.925 | 1.985 | 0.13 | 0.22 | 0.22 | 0.22 | 0.20 | 0.05 | 0.19 | 1.320 | |||||
Test Device 6: J23117+I13504 (Colony 1) | 0.046 | 0.047 | 0.047 | 0.047 | 13.49 | 12.07 | 12.23 | 12.83 | 0.00775 | 0.00875 | 0.00875 | 0.00875 | 1.705 | 0.285 | 0.445 | 1.045 | 0.19 | 0.03 | 0.04 | 0.10 | 0.09 | 0.07 | 0.07 | 2.353 | |||||
Test Device 6: J23117+I13504 (Colony 2) | 0.046 | 0.044 | 0.044 | 0.044 | 13.24 | 12.34 | 13.56 | 13.93 | 0.00775 | 0.00575 | 0.00575 | 0.00575 | 1.455 | 0.555 | 1.775 | 2.145 | 0.16 | 0.08 | 0.27 | 0.33 | 0.21 | 0.11 | 0.19 | 1.829 | |||||
Hour 2: | |||||||||||||||||||||||||||||
Negative Control (Colony 1) | 0.055 | 0.068 | 0.054 | 0.053 | 13.36 | 12.83 | 12.3 | 12.85 | 0.01675 | 0.02975 | 0.01575 | 0.01475 | 1.575 | 1.045 | 0.515 | 1.065 | 0.08 | 0.03 | 0.03 | 0.06 | 0.05 | 0.03 | 0.05 | 1.690 | |||||
Negative Control (Colony 2) | 0.232 | 0.209 | 0.148 | 0.054 | 13.54 | 12.85 | 13.88 | 11.16 | 0.19375 | 0.17075 | 0.10975 | 0.01575 | 1.755 | 1.065 | 2.095 | -0.625 | 0.01 | 0.01 | 0.02 | -0.03 | 0.00 | 0.02 | #NUM! | #NUM! | |||||
Positive Control (Colony 1) | 0.058 | 0.053 | 0.053 | 0.052 | 20.39 | 19.68 | 20.97 | 21 | 0.01975 | 0.01475 | 0.01475 | 0.01375 | 8.605 | 7.895 | 9.185 | 9.215 | 0.38 | 0.47 | 0.55 | 0.59 | 0.50 | 0.09 | 0.49 | 1.210 | |||||
Positive Control (Colony 2) | 0.152 | 0.104 | 0.053 | 0.054 | 21.64 | 20.63 | 19.6 | 20.02 | 0.11375 | 0.06575 | 0.01475 | 0.01575 | 9.855 | 8.845 | 7.815 | 8.235 | 0.08 | 0.12 | 0.46 | 0.46 | 0.28 | 0.21 | 0.21 | 2.540 | |||||
Test Device 1: J23101.BCD2.E0040.B0015 (Colony 1) | 0.051 | 0.054 | 0.049 | 0.049 | 26.75 | 29.96 | 29.37 | 29.77 | 0.01275 | 0.01575 | 0.01075 | 0.01075 | 14.965 | 18.175 | 17.585 | 17.985 | 1.03 | 1.01 | 1.43 | 1.47 | 1.24 | 0.25 | 1.22 | 1.226 | |||||
Test Device 1: J23101.BCD2.E0040.B0015 (Colony 2) | 0.204 | 0.193 | 0.195 | 0.136 | 29.84 | 32.35 | 33.21 | 26.81 | 0.16575 | 0.15475 | 0.15675 | 0.09775 | 18.055 | 20.565 | 21.425 | 15.025 | 0.10 | 0.12 | 0.12 | 0.13 | 0.12 | 0.02 | 0.12 | 1.154 | |||||
Test Device 2: J23106.BCD2.E0040.B0015 (Colony 1) | 0.052 | 0.05 | 0.05 | 0.05 | 31.21 | 29.42 | 29.86 | 31.01 | 0.01375 | 0.01175 | 0.01175 | 0.01175 | 19.425 | 17.635 | 18.075 | 19.225 | 1.24 | 1.32 | 1.35 | 1.43 | 1.33 | 0.08 | 1.33 | 1.063 | |||||
Test Device 2: J23106.BCD2.E0040.B0015 (Colony 2) | 0.054 | 0.076 | 0.053 | 0.052 | 30.14 | 35.8 | 34.28 | 31.55 | 0.01575 | 0.03775 | 0.01475 | 0.01375 | 18.355 | 24.015 | 22.495 | 19.765 | 1.02 | 0.56 | 1.34 | 1.26 | 1.04 | 0.35 | 0.99 | 1.491 | |||||
Test Device 3: J23117.BCD2.E0040.B0015 (Colony 1) | 0.054 | 0.055 | 0.057 | 0.054 | 14.69 | 14.47 | 16.36 | 16.08 | 0.01575 | 0.01675 | 0.01875 | 0.01575 | 2.905 | 2.685 | 4.575 | 4.295 | 0.16 | 0.14 | 0.21 | 0.24 | 0.19 | 0.05 | 0.18 | 1.278 | |||||
Test Device 3: J23117.BCD2.E0040.B0015 (Colony 2) | 0.055 | 0.054 | 0.053 | 0.052 | 17.35 | 16.78 | 18.05 | 15.31 | 0.01675 | 0.01575 | 0.01475 | 0.01375 | 5.565 | 4.995 | 6.265 | 3.525 | 0.29 | 0.28 | 0.37 | 0.22 | 0.29 | 0.06 | 0.29 | 1.230 | |||||
Test Device 4: J23101+I13504 (Colony 1) | 0.054 | 0.053 | 0.052 | 0.052 | 21.9 | 22.44 | 23.34 | 23.84 | 0.01575 | 0.01475 | 0.01375 | 0.01375 | 10.115 | 10.655 | 11.555 | 12.055 | 0.56 | 0.63 | 0.74 | 0.77 | 0.68 | 0.09 | 0.67 | 1.154 | |||||
Test Device 4: J23101+I13504 (Colony 2) | 0.051 | 0.052 | 0.051 | 0.05 | 25.2 | 25.93 | 25.8 | 24.39 | 0.01275 | 0.01375 | 0.01275 | 0.01175 | 13.415 | 14.145 | 14.015 | 12.605 | 0.92 | 0.90 | 0.96 | 0.94 | 0.93 | 0.03 | 0.93 | 1.029 | |||||
Test Device 5: J23106+I13504 (Colony 1) | 0.055 | 0.053 | 0.051 | 0.053 | 22.94 | 22.74 | 21.48 | 21.35 | 0.01675 | 0.01475 | 0.01275 | 0.01475 | 11.155 | 10.955 | 9.695 | 9.565 | 0.58 | 0.65 | 0.67 | 0.57 | 0.62 | 0.05 | 0.62 | 1.082 | |||||
Test Device 5: J23106+I13504 (Colony 2) | 0.057 | 0.056 | 0.055 | 0.055 | 22.02 | 22.25 | 22.51 | 21.09 | 0.01875 | 0.01775 | 0.01675 | 0.01675 | 10.235 | 10.465 | 10.725 | 9.305 | 0.48 | 0.52 | 0.56 | 0.49 | 0.51 | 0.04 | 0.51 | 1.075 | |||||
Test Device 6: J23117+I13504 (Colony 1) | 0.053 | 0.054 | 0.056 | 0.055 | 16.19 | 15.64 | 16.24 | 16.07 | 0.01475 | 0.01575 | 0.01775 | 0.01675 | 4.405 | 3.855 | 4.455 | 4.285 | 0.26 | 0.21 | 0.22 | 0.22 | 0.23 | 0.02 | 0.23 | 1.094 | |||||
Test Device 6: J23117+I13504 (Colony 2) | 0.051 | 0.051 | 0.049 | 0.05 | 16.13 | 16.2 | 16.26 | 17.05 | 0.01275 | 0.01275 | 0.01075 | 0.01175 | 4.345 | 4.415 | 4.475 | 5.265 | 0.30 | 0.30 | 0.36 | 0.39 | 0.34 | 0.05 | 0.34 | 1.145 | |||||
Hour 4: | |||||||||||||||||||||||||||||
Negative Control (Colony 1) | 0.191 | 0.15 | 0.134 | 0.139 | 14.45 | 15.48 | 14.63 | 13.73 | 0.15275 | 0.11175 | 0.09575 | 0.10075 | 2.665 | 3.695 | 2.845 | 1.945 | 0.02 | 0.03 | 0.03 | 0.02 | 0.02 | 0.01 | 0.02 | 1.370 | |||||
Negative Control (Colony 2) | 0.147 | 0.161 | 0.151 | 0.142 | 14.89 | 15.77 | 16.32 | 15.74 | 0.10875 | 0.12275 | 0.11275 | 0.10375 | 3.105 | 3.985 | 4.535 | 3.955 | 0.03 | 0.03 | 0.04 | 0.03 | 0.03 | 0.00 | 0.03 | 1.169 | |||||
Positive Control (Colony 1) | 0.184 | 0.139 | 0.13 | 0.127 | 50.64 | 49.12 | 44.43 | 39.3 | 0.14575 | 0.10075 | 0.09175 | 0.08875 | 38.855 | 37.335 | 32.645 | 27.515 | 0.23 | 0.32 | 0.31 | 0.27 | 0.29 | 0.04 | 0.28 | 1.161 | |||||
Positive Control (Colony 2) | 0.157 | 0.14 | 0.133 | 0.134 | 58.28 | 57.94 | 52.46 | 52.37 | 0.11875 | 0.10175 | 0.09475 | 0.09575 | 46.495 | 46.155 | 40.675 | 40.585 | 0.34 | 0.40 | 0.38 | 0.37 | 0.37 | 0.02 | 0.37 | 1.063 | |||||
Test Device 1: J23101.BCD2.E0040.B0015 (Colony 1) | 0.057 | 0.056 | 0.055 | 0.054 | 48.17 | 41.93 | 44.44 | 43.61 | 0.01875 | 0.01775 | 0.01675 | 0.01575 | 36.385 | 30.145 | 32.655 | 31.825 | 1.70 | 1.49 | 1.71 | 1.77 | 1.67 | 0.12 | 1.66 | 1.079 | |||||
Test Device 1: J23101.BCD2.E0040.B0015 (Colony 2) | 0.056 | 0.054 | 0.054 | 0.052 | 46.69 | 50.03 | 47.27 | 45.78 | 0.01775 | 0.01575 | 0.01575 | 0.01375 | 34.905 | 38.245 | 35.485 | 33.995 | 1.72 | 2.13 | 1.97 | 2.17 | 2.00 | 0.20 | 1.99 | 1.110 | |||||
Test Device 2: J23106.BCD2.E0040.B0015 (Colony 1) | 0.143 | 0.13 | 0.129 | 0.116 | 76.83 | 75.98 | 72.69 | 70.29 | 0.10475 | 0.09175 | 0.09075 | 0.07775 | 65.045 | 64.195 | 60.905 | 58.505 | 0.54 | 0.61 | 0.59 | 0.66 | 0.60 | 0.05 | 0.60 | 1.084 | |||||
Test Device 2: J23106.BCD2.E0040.B0015 (Colony 2) | 0.131 | 0.106 | 0.1 | 0.096 | 75.71 | 70.61 | 69.71 | 75.01 | 0.09275 | 0.06775 | 0.06175 | 0.05775 | 63.925 | 58.825 | 57.925 | 63.225 | 0.60 | 0.76 | 0.82 | 0.96 | 0.79 | 0.15 | 0.78 | 1.213 | |||||
Test Device 3: J23117.BCD2.E0040.B0015 (Colony 1) | 0.208 | 0.152 | 0.144 | 0.126 | 18.56 | 17.22 | 16.66 | 15.38 | 0.16975 | 0.11375 | 0.10575 | 0.08775 | 6.775 | 5.435 | 4.875 | 3.595 | 0.03 | 0.04 | 0.04 | 0.04 | 0.04 | 0.00 | 0.04 | 1.092 | |||||
Test Device 3: J23117.BCD2.E0040.B0015 (Colony 2) | 0.155 | 0.131 | 0.114 | 0.115 | 16.97 | 16.11 | 16.05 | 17 | 0.11675 | 0.09275 | 0.07575 | 0.07675 | 5.185 | 4.325 | 4.265 | 5.215 | 0.04 | 0.04 | 0.05 | 0.06 | 0.05 | 0.01 | 0.05 | 1.214 | |||||
Test Device 4: J23101+I13504 (Colony 1) | 0.115 | 0.118 | 0.099 | 0.1 | 46.38 | 44.29 | 43.31 | 42.04 | 0.07675 | 0.07975 | 0.06075 | 0.06175 | 34.595 | 32.505 | 31.525 | 30.255 | 0.40 | 0.36 | 0.45 | 0.43 | 0.41 | 0.04 | 0.41 | 1.111 | |||||
Test Device 4: J23101+I13504 (Colony 2) | 0.107 | 0.103 | 0.099 | 0.094 | 46.3 | 44.96 | 44.81 | 42.31 | 0.06875 | 0.06475 | 0.06075 | 0.05575 | 34.515 | 33.175 | 33.025 | 30.525 | 0.44 | 0.45 | 0.48 | 0.48 | 0.46 | 0.02 | 0.46 | 1.044 | |||||
Test Device 5: J23106+I13504 (Colony 1) | 0.119 | 0.112 | 0.122 | 0.117 | 24.84 | 25.74 | 27.41 | 27.58 | 0.08075 | 0.07375 | 0.08375 | 0.07875 | 13.055 | 13.955 | 15.625 | 15.795 | 0.14 | 0.17 | 0.16 | 0.18 | 0.16 | 0.01 | 0.16 | 1.096 | |||||
Test Device 5: J23106+I13504 (Colony 2) | 0.139 | 0.125 | 0.147 | 0.135 | 24.09 | 22.96 | 26.29 | 26.06 | 0.10075 | 0.08675 | 0.10875 | 0.09675 | 12.305 | 11.175 | 14.505 | 14.275 | 0.11 | 0.11 | 0.12 | 0.13 | 0.12 | 0.01 | 0.12 | 1.083 | |||||
Test Device 6: J23117+I13504 (Colony 1) | 0.137 | 0.145 | 0.142 | 0.125 | 13.65 | 14.24 | 13.76 | 14.66 | 0.09875 | 0.10675 | 0.10375 | 0.08675 | 1.865 | 2.455 | 1.975 | 2.875 | 0.02 | 0.02 | 0.02 | 0.03 | 0.02 | 0.01 | 0.02 | 1.301 | |||||
Test Device 6: J23117+I13504 (Colony 2) | 0.11 | 0.111 | 0.11 | 0.116 | 14.42 | 14.08 | 13.93 | 14.6 | 0.07175 | 0.07275 | 0.07175 | 0.07775 | 2.635 | 2.295 | 2.145 | 2.815 | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 | 0.00 | 0.03 | 1.107 | |||||
Hour 6: | |||||||||||||||||||||||||||||
Negative Control (Colony 1) | 0.403 | 0.396 | 0.385 | 0.384 | 13.86 | 14.13 | 14.1 | 13.53 | 0.36475 | 0.35775 | 0.34675 | 0.34575 | 2.075 | 2.345 | 2.315 | 1.745 | 0.00 | 0.01 | 0.01 | 0.00 | 0.01 | 0.00 | 0.01 | 1.140 | |||||
Negative Control (Colony 2) | 0.282 | 0.399 | 0.446 | 0.312 | 13.67 | 13.87 | 14.02 | 13.94 | 0.24375 | 0.36075 | 0.40775 | 0.27375 | 1.885 | 2.085 | 2.235 | 2.155 | 0.01 | 0.01 | 0.00 | 0.01 | 0.01 | 0.00 | 0.01 | 1.209 | |||||
Positive Control (Colony 1) | 0.308 | 0.279 | 0.297 | 0.282 | 84.88 | 74.53 | 79.93 | 79.25 | 0.26975 | 0.24075 | 0.25875 | 0.24375 | 73.095 | 62.745 | 68.145 | 67.465 | 0.24 | 0.23 | 0.23 | 0.24 | 0.23 | 0.01 | 0.23 | 1.028 | |||||
Positive Control (Colony 2) | 0.393 | 0.303 | 0.237 | 0.411 | 79.7 | 68.25 | 81.04 | 81.93 | 0.35475 | 0.26475 | 0.19875 | 0.37275 | 67.915 | 56.465 | 69.255 | 70.145 | 0.17 | 0.19 | 0.31 | 0.16 | 0.21 | 0.07 | 0.20 | 1.336 | |||||
Test Device 1: J23101.BCD2.E0040.B0015 (Colony 1) | 0.069 | 0.059 | 0.068 | 0.057 | 53.36 | 48.94 | 53.32 | 52.33 | 0.03075 | 0.02075 | 0.02975 | 0.01875 | 41.575 | 37.155 | 41.535 | 40.545 | 1.19 | 1.57 | 1.22 | 1.90 | 1.47 | 0.33 | 1.44 | 1.248 | |||||
Test Device 1: J23101.BCD2.E0040.B0015 (Colony 2) | 0.07 | 0.069 | 0.068 | 0.068 | 54.14 | 55.06 | 56.59 | 55.34 | 0.03175 | 0.03075 | 0.02975 | 0.02975 | 42.355 | 43.275 | 44.805 | 43.555 | 1.17 | 1.23 | 1.32 | 1.28 | 1.25 | 0.07 | 1.25 | 1.054 | |||||
Test Device 2: J23106.BCD2.E0040.B0015 (Colony 1) | 0.308 | 0.307 | 0.288 | 0.324 | 146.1 | 129 | 142.7 | 146.6 | 0.26975 | 0.26875 | 0.24975 | 0.28575 | 134.315 | 117.215 | 130.915 | 134.815 | 0.44 | 0.38 | 0.46 | 0.41 | 0.42 | 0.03 | 0.42 | 1.082 | |||||
Test Device 2: J23106.BCD2.E0040.B0015 (Colony 2) | 0.403 | 0.427 | 0.217 | 0.287 | 125.9 | 120.6 | 132.6 | 121.6 | 0.36475 | 0.38875 | 0.17875 | 0.24875 | 114.115 | 108.815 | 120.815 | 109.815 | 0.27 | 0.25 | 0.59 | 0.39 | 0.37 | 0.16 | 0.35 | 1.487 | |||||
Test Device 3: J23117.BCD2.E0040.B0015 (Colony 1) | 0.398 | 0.399 | 0.392 | 0.343 | 16.24 | 15.33 | 14.79 | 16.13 | 0.35975 | 0.36075 | 0.35375 | 0.30475 | 4.455 | 3.545 | 3.005 | 4.345 | 0.01 | 0.01 | 0.01 | 0.01 | 0.01 | 0.00 | 0.01 | 1.261 | |||||
Test Device 3: J23117.BCD2.E0040.B0015 (Colony 2) | 0.3 | 0.295 | 0.209 | 0.181 | 15.95 | 15.66 | 15.93 | 15.44 | 0.26175 | 0.25675 | 0.17075 | 0.14275 | 4.165 | 3.875 | 4.145 | 3.655 | 0.01 | 0.01 | 0.02 | 0.02 | 0.02 | 0.00 | 0.02 | 1.318 | |||||
Test Device 4: J23101+I13504 (Colony 1) | 0.272 | 0.274 | 0.264 | 0.264 | 87.02 | 86.88 | 85.79 | 83.04 | 0.23375 | 0.23575 | 0.22575 | 0.22575 | 75.235 | 75.095 | 74.005 | 71.255 | 0.28 | 0.28 | 0.29 | 0.28 | 0.28 | 0.00 | 0.28 | 1.016 | |||||
Test Device 4: J23101+I13504 (Colony 2) | 0.296 | 0.236 | 0.225 | 0.228 | 97.47 | 97.03 | 97.78 | 82.43 | 0.25775 | 0.19775 | 0.18675 | 0.18975 | 85.685 | 85.245 | 85.995 | 70.645 | 0.29 | 0.38 | 0.40 | 0.33 | 0.35 | 0.05 | 0.35 | 1.158 | |||||
Test Device 5: J23106+I13504 (Colony 1) | 0.36 | 0.366 | 0.368 | 0.347 | 47.54 | 46.09 | 45.93 | 42.66 | 0.32175 | 0.32775 | 0.32975 | 0.30875 | 35.755 | 34.305 | 34.145 | 30.875 | 0.10 | 0.09 | 0.09 | 0.09 | 0.09 | 0.00 | 0.09 | 1.045 | |||||
Test Device 5: J23106+I13504 (Colony 2) | 0.327 | 0.324 | 0.294 | 0.309 | 36.82 | 41.73 | 41.66 | 37.26 | 0.28875 | 0.28575 | 0.25575 | 0.27075 | 25.035 | 29.945 | 29.875 | 25.475 | 0.08 | 0.09 | 0.10 | 0.08 | 0.09 | 0.01 | 0.09 | 1.138 | |||||
Test Device 6: J23117+I13504 (Colony 1) | 0.387 | 0.402 | 0.38 | 0.343 | 14.5 | 14.72 | 14.73 | 14.63 | 0.34875 | 0.36375 | 0.34175 | 0.30475 | 2.715 | 2.935 | 2.945 | 2.845 | 0.01 | 0.01 | 0.01 | 0.01 | 0.01 | 0.00 | 0.01 | 1.083 | |||||
Test Device 6: J23117+I13504 (Colony 2) | 0.351 | 0.374 | 0.356 | 0.375 | 14.53 | 14.82 | 14.32 | 14.82 | 0.31275 | 0.33575 | 0.31775 | 0.33675 | 2.745 | 3.035 | 2.535 | 3.035 | 0.01 | 0.01 | 0.01 | 0.01 | 0.01 | 0.00 | 0.01 | 1.060 |
Requirements
- 2L Erlenmeyer flask
- LB broth powder
- Bacteriological Agar
- Distilled or milliQ water
- Magnetic stirrer and bead(optional)
- Measuring cylinder
- Autoclave tape
- Cotton plug(for the flask)
- Aluminium foil
- Autoclaving machine
- Petri plates
Procedure
- Prepare 1L of LB liquid (25g broth powder per 1L) in a 2000mL Erlenmeyer flask.
- Add 15g (1.5%) bacteriological agar
- Use magnetic stirrer and bead to mix , if not available simply swirl to mix. Powder will not dissolve completely.
- Make a nice cotton plug by rolling and folding a big piece of cotton and put it on the mouth of the flask(Try pulling it out once, if it makes a noise then, Bravo!)
- Add a fresh piece of aluminum foil to cover the top, and add a fresh piece of autoclave tape.
- Autoclave at 121°C
- After cycle is complete, remove to benchtop to cool to ~50 C. (As a ballpark, this is still quite warm, but cool enough to leave your gloved hand on the outside of the flask for several seconds without burning it. The media should still be liquid but should be just beginning to thicken)
- If you are adding antibiotics, add them at this point, and swirl the flask vigorously for several seconds to mix (1000 times dilution of the stock solution)
- Set out your plates, and label the bottom with the appropriate antibiotic: A=ampicillin, C=chloramphenicol, K=kanamycin, T=tetracycline, S=streptomycin
- Pour out the media into petri dishes as follows: Remove the lid to a dish, and remove the foil cover to the flask. Pour just enough LB agar into the petri dish to completely cover the bottom of the dish, then replace the lid.
- Finish pouring dishes within 5 minutes of adding the antibiotic.
- Dishes should be stored UPSIDE DOWN in the cold room.
Requirements
- 1L autoclave bottle or erlenmeyer flask
- 6.25g LB broth powder
- 2.5mM KCl
- 20mM MgSO 4 .7H 2 O
- Distilled or milliQ water
- Magnetic stirrer and bead(optional)
- Measuring cylinder
- Autoclave tape
- Cotton plug(for the flask)
- Aluminium foil
- Autoclaving machine
Procedure
- In a 1L autoclave bottle or flask , add 6.25g LB broth powder.
- Calculate and weigh 2.5mM KCl and 20mM MgSO 4 .7H 2 O.
- Adjust pH to 7.5(using NaOH)
- Add distilled water to make the solution upto 250mL.
- Either Swirl or use magnetic stirrer and a bead to mix(might not dissolve completely) .
- Make a nice cotton plug by rolling and folding a big piece of cotton and put it on the mouth of the flask.(Try pulling it out once, if it makes a noise then, Bravo!)
- Put an Aluminium foil on top of it and a piece of autoclave tape
- Keep the cap slightly open if using a bottle.
- Autoclave at 121°C
- Cool to RT before use. Do not tighten cap until cool.
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