Difference between revisions of "Team:RHIT/InterLab"

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<h1>InterLab</h1>
 
<h1>InterLab</h1>
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<h3>Bronze Medal Criterion #4</h3>
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<p>The Interlaboratory Measurement Study was an aspect of iGEM that seeks to standardize and improve measurement in the synthetic biology community. In 2017, the interlab study centered upon comparing fluorescence data over a broad range of locations, laboratories and equipment.. Participating iGEM teams were tasked with measuring GFP fluorescence from constructed testing devices in the parts distribution and collecting a fluorescein standard curve.
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<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or improve the characterization of an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2017 part number range.
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For teams participating in the <a href="https://2017.igem.org/Competition/InterLab_Study">InterLab study</a>, all work must be shown on this page.
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To measure the fluorescence and absorbance of our assays, plate readers at the university - Rose-Hulman Institute of Technology were used. Two separate plate readers were necessary: *name* plate reader was used obtain absorbance measurements with Gen5 software, and the *name* plate reader to measure the fluorescence with Softmax Pro 4.6. OD630 measurements were substituted for OD600 due to limitations in resources- no plate reader was available with the filter for OD600 measurements. All fluorescence measurements were taken with an excitation wavelength of 485 nm and an emission wavelength of 530 nm.
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Data collected can be found *here* <br>
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All lab notebook for the interlab study pages can be found *here*
 
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Revision as of 23:50, 31 October 2017

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InterLab

The Interlaboratory Measurement Study was an aspect of iGEM that seeks to standardize and improve measurement in the synthetic biology community. In 2017, the interlab study centered upon comparing fluorescence data over a broad range of locations, laboratories and equipment.. Participating iGEM teams were tasked with measuring GFP fluorescence from constructed testing devices in the parts distribution and collecting a fluorescein standard curve.

To measure the fluorescence and absorbance of our assays, plate readers at the university - Rose-Hulman Institute of Technology were used. Two separate plate readers were necessary: *name* plate reader was used obtain absorbance measurements with Gen5 software, and the *name* plate reader to measure the fluorescence with Softmax Pro 4.6. OD630 measurements were substituted for OD600 due to limitations in resources- no plate reader was available with the filter for OD600 measurements. All fluorescence measurements were taken with an excitation wavelength of 485 nm and an emission wavelength of 530 nm.

Data collected can be found *here*
All lab notebook for the interlab study pages can be found *here*