<p align="justify">The aiiA gene was of such relevance to the project's development because of it's function, since it's responsible for the inhibition of two of <i>Erwinia amylovora</i>'s main virulence factors: the type III secretion system and the production of exopolysaccharides. Quorum sensing regulates, among many other cell functions, both of these; therefore, by disrupting the quorum sensing, the bacteria will lose these virulence factors. The epsE gene, in the same way, constitutes a fundamental part of the project, since it eliminates cell motility by promoting the separation of the flagellum from the motor proteins located in the cell membrane. Each BioBrick® presented in this section is a new and functional addition to iGEM's Parts Registry, since both's codifying genes' sequences are different from the ones on the catalog (and are confirmed to codify for their respective proteins, unlike the parts that are already in the Parts Registry, which are reported as being putative) and were synthesized thanks to the sponsorship granted by IDT®. These two new BioBricks® (aiiA: BBa_K2471004 and epsE: BBa_K2471005) were transformed into <i>Escherichia coli</i> BL21(DE3) (both) and <i>Erwinia amylovora</i> (just aiiA). After performing polyacrylamide gel electrophoresis, it was concluded that our new aiiA BioBrick® is functional because the protein was expressed in both bacterial strains that it was transformed into; on the other hand, the epsE BioBrick® didn't work, since no protein expression was found in <i>Escherichia coli</i> BL21(DE3).</p><br><br><br>