Difference between revisions of "Team:UChicago/Description"

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{{UChicago}}
 
{{UChicago}}
 
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<img src="https://static.igem.org/mediawiki/2017/c/c5/T--UChicago--descritrans.png"  id="title">
  
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<h1>Description</h1>
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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<h5>What should this page contain?</h5>
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<li> A clear and concise description of your project.</li>
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
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<h1>Background</h1>
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  Plasmid vectors are invaluable tools for synthetic biologists and the broader scientific community. For those who work with yeast, one particularly useful vector is the centromeric plasmid: a vector that, by essentially mimicking a yeast chromosome, is able to provide both the flexibility of replicating plasmids and the stability of integrating plasmids.<br>
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  Organisms often do not retain plasmids once they have been transformed. Plasmid loss occurs when cells undergo replication. During mitosis, microtubules attach to the centromere of DNA and distribute it evenly among the daughter cells. If we were to insert a centromeric sequence into a plasmid, the microtubules would attach to this region of DNA. As a result, the plasmid would be distributed much more equally (and without loss) among the daughter cells in mitosis.
  
 
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<h5>Advice on writing your Project Description</h5>
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  While there are multiple centromere plasmid options for <i>Saccharomyces cerevisiae</i>, the scientific community has yet to design a yeast centromere series for the other widely-used yeast strain, <i>Pichia pastoris</i>. In response, <b>UChicago Genehackers has set out to design the first yeast centromeric plasmid for <i>Pichia pastoris</i></b>.
  
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.
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<h1>Solution</h1>
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
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  We will identify the minimal, essential region of the Pichia centromere that, when incorporated into a plasmid backbone, will allow the plasmid to mimic a Pichia chromosome during replication. This region can then be incorporated into Pichia backbones to convert them into centromere plasmids, which transform with high efficiency, have low and constant copy numbers, and are stably maintained without integration. Ultimately, we will contribute an important foundational tool for flexible, creative, and impactful cloning in yeast. If successful, the centromeric plasmids will serve a multitude of purposes in the bioengineering and industrial fields.
  
 
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
 
  
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<a href="mailto:genehackers.uchicago@gmail.com">GeneHackers 2017</a>
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<h5>Inspiration</h5>
 
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<p>See how other teams have described and presented their projects: </p>
 
  
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<li><a href="https://2016.igem.org/Team:Imperial_College/Description">2016 Imperial College</a></li>
 
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<li><a href="https://2016.igem.org/Team:Wageningen_UR/Description">2016 Wageningen UR</a></li>
 
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> 2014 UC Davis</a></li>
 
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">2014 SYSU Software</a></li>
 
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Latest revision as of 03:02, 1 November 2017

HOME
TEAM
PROJECT
Description
Design
Experiments
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Results
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PARTS
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PUBLIC ENGAGEMENT

Background

Plasmid vectors are invaluable tools for synthetic biologists and the broader scientific community. For those who work with yeast, one particularly useful vector is the centromeric plasmid: a vector that, by essentially mimicking a yeast chromosome, is able to provide both the flexibility of replicating plasmids and the stability of integrating plasmids.
Organisms often do not retain plasmids once they have been transformed. Plasmid loss occurs when cells undergo replication. During mitosis, microtubules attach to the centromere of DNA and distribute it evenly among the daughter cells. If we were to insert a centromeric sequence into a plasmid, the microtubules would attach to this region of DNA. As a result, the plasmid would be distributed much more equally (and without loss) among the daughter cells in mitosis.

Project

While there are multiple centromere plasmid options for Saccharomyces cerevisiae, the scientific community has yet to design a yeast centromere series for the other widely-used yeast strain, Pichia pastoris. In response, UChicago Genehackers has set out to design the first yeast centromeric plasmid for Pichia pastoris.

Solution

We will identify the minimal, essential region of the Pichia centromere that, when incorporated into a plasmid backbone, will allow the plasmid to mimic a Pichia chromosome during replication. This region can then be incorporated into Pichia backbones to convert them into centromere plasmids, which transform with high efficiency, have low and constant copy numbers, and are stably maintained without integration. Ultimately, we will contribute an important foundational tool for flexible, creative, and impactful cloning in yeast. If successful, the centromeric plasmids will serve a multitude of purposes in the bioengineering and industrial fields.
GeneHackers 2017