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  We will identify the minimal, essential region of the Pichia centromere that, when incorporated into a plasmid backbone, will allow the plasmid to mimic a Pichia chromosome during replication. This region can then be incorporated into Pichia backbones to convert them into centromere plasmids, which transform with high efficiency, have low and constant copy numbers, and are stably maintained without integration. Ultimately, we will contribute an important foundational tool for flexible, creative, and impactful cloning in yeast. If successful, the centromeric plasmids will serve a multitude of purposes in the bioengineering and industrial fields.
 
  
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All proteins expressed in this project are relatively harmless and nontoxic. We used well-known, safe lab protocols for our assays.<br>
 
All proteins expressed in this project are relatively harmless and nontoxic. We used well-known, safe lab protocols for our assays.<br>
 
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<a href="mailto:genehackers.uchicago@gmail.com">GeneHackers 2017</a>
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Latest revision as of 03:04, 1 November 2017

HOME
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PROJECT
Description
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PARTS
SAFETY
PUBLIC ENGAGEMENT
Our team works with a limited number of toxic or harmful chemicals such as ethidium bromide and bleach. Team members are required to wear rubber gloves at all times in the lab.


In the case of a spill, liquids are soaked up and the lab bench and any affected areas wiped down and cleaned.


Biohazardous waste materials are collected in special waste bins in the lab that will be treated by the University's hazardous waste disposal contractor.


Cells cultures are killed with bleach before disposal, and general solid waste is collected into biohazard bags and disposed of.


All members are required to complete University-issued biosafety training appropriate to the Biosafety 1 classification of our lab. This training covered requirements for laboratory practices and equipment, biohazard containment, recombinant DNA, bioethics, fire safety, and personal protective equipment.


We hold weekly meetings with faculty and graduate student advisors so that experienced wet lab scientists can provide advice and feedback on safe lab practices. Graduate student advisors are also available during lab hours to provide immediate assistance.


All proteins expressed in this project are relatively harmless and nontoxic. We used well-known, safe lab protocols for our assays.
GeneHackers 2017