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| − | <img src="https://static.igem.org/mediawiki/2017/a/a3/T--UChicago--titletransparent.png" id="title"> | + | <img src="https://static.igem.org/mediawiki/2017/2/2d/T--UChicago--ppp.png" id="title"> |
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| − | <a data-scroll href="#p1"> | + | |
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| + | <a data-scroll href="#p1" class="arrow"></ a> |
| − | </a>
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| | </div> | | </div> |
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| − | <div class="paragraph"id="p1"> | + | <h1>About GeneHackers</h1> |
| − | <p>Plasmid vectors are invaluable tools for synthetic biologists and the broader scientific community. For those who work with yeast, one particularly useful vector is the centromeric plasmid: a vector that, by essentially mimicking a yeast chromosome, is able to provide both the flexibility of replicating plasmids and the stability of integrating plasmids.</p>
| + | <div class="paragraph" id="p1"> |
| | + | Genehackers is a recognized undergraduate student association with The University of Chicago. Founded in 2013, our organization aims to raise awareness about synthetic biology through community and research. We have competed in the iGem competition since the founding of our group. Our team has created innovative projects that bring synthetic biology to the center of the laboratory experience. |
| | </div> | | </div> |
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| − | <img src="https://static.igem.org/mediawiki/2017/7/7c/T--UChicago--circles.jpg" class="circlepics">
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| − | <div class="paragraph"id="p2"> | + | <div class="paragraph" id="top"> |
| − | <p>Organisms often do not retain plasmids once they have been transformed. Plasmid loss occurs when cells undergo replication. During mitosis, microtubules attach to the centromere of DNA and distribute it evenly among the daughter cells. If we were to insert a centromeric sequence into a plasmid, the microtubules would attach to this region of DNA. As a result, the plasmid would be distributed much more equally (and without loss) among the daughter cells in mitosis.
| + | This year, UChicago Genehackers has set out to design the first yeast centromeric plasmid for <i>Pichia pastoris</i>. |
| − | </p> | + | |
| | </div> | | </div> |
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| − | <img src="https://static.igem.org/mediawiki/2017/7/7c/T--UChicago--circles.jpg" class="circlepics">
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| − | <div class="paragraph"id="p3"> | + | <h1>iGem 2017</h1> |
| − | <p>While there are multiple centromere plasmid options for <i>Saccharomyces cerevisiae</i>, the scientific community has yet to design a yeast centromere series for the other widely-used yeast strain, <i>Pichia pastoris</i>. In response, <b>UChicago Genehackers has set out to design the first yeast centromeric plasmid for <i>Pichia pastoris</i></b>.
| + | <div class="paragraph"> |
| − | </p>
| + | Our team has already made significant strides towards creating the proposed centromeric plasmid vector. We have grown psB1C3mut in LB+CAM (chloramphenicol), psD000 in LB+CAM and drop-out arginine plates and psD001 in LB+CAM. Additionally, we have amplified five potential centromeric regions in total from the chromosomal DNA of Pichia pastors. |
| | </div> | | </div> |
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| − | <img src="https://static.igem.org/mediawiki/2017/7/7c/T--UChicago--circles.jpg" class="circlepics">
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| − | <div class="paragraph"id="p3">
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| − | <p>We will identify the minimal, essential region of the Pichia centromere that, when incorporated into a plasmid backbone, will allow the plasmid to mimic a Pichia chromosome during replication. This region can then be incorporated into Pichia backbones to convert them into centromere plasmids, which transform with high efficiency, have low and constant copy numbers, and are stably maintained without integration. Ultimately, we will contribute an important foundational tool for flexible, creative, and impactful cloning in yeast. If successful, the centromeric plasmids will serve a multitude of purposes in the bioengineering and industrial fields.
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| | + | #bodyContent h1{ |
| | + | text-align: center; |
| | + | font-family: 'Open Sans', sans-serif; |
| | + | font-size: 30px; |
| | + | color: rgb(0,109,171); |
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| | + | margin: auto; |
| | + | width: 50%; |
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| | + | #top{ |
| | + | width: 650px; |
| | + | font-size: 50px; |
| | + | margin: auto; |
| | + | margin-top: 50px; |
| | + | color: white; |
| | + | background-color: grey; |
| | + | height: 400px; |
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| | + | #top:hover |
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| | + | background-color: rgba(0,109,171, .4);} |
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| − | font-size: 40px; | + | padding-top: 15px; |
| | + | padding-bottom: 15px; |
| | + | padding-right: 30px; |
| | + | text-align: left; |
| | + | font-size: 20px; |
| | margin-right: 30px; | | margin-right: 30px; |
| − | margin-left: 30px; | + | margin-left: 20px; |
| | + | padding-left: 20px; |
| | + | line-height: 1.5; |
| | + | text-align: center; |
| | } | | } |
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| | + | #bodyContent{ |
| | + | padding-right: 15%; |
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| | </style> | | </style> |
| − |
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| | + | <div class="circles"> |
| | + | <div> |
| | + | <a class="btn" id="pencil" href="https://2017.igem.org/Team:UChicago/Description"><i class="ion-iso-arrow-down"></i></a> |
| | + | </div> |
| | | | |
| − | <div class="column half_size" > | + | <div> |
| − | <h5>Before you start: </h5>
| + | <a class="btn" id="gavel" href="https://2017.igem.org/Team:UChicago/Parts"><i class="ion-ios-arrow-down"></i></a> |
| − | <p> Please read the following pages:</p>
| + | |
| − | <ul>
| + | |
| − | <li> <a href=" https://2017.igem.org/Competition" >Competition Hub</a> </li> | + | |
| − | <li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
| + | |
| − | <li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
| + | |
| − | </ul>
| + | |
| | </div> | | </div> |
| | | | |
| − | <div class="column half_size" >
| |
| − | <div class="highlight">
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| − | <h5> Styling your wiki </h5>
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| − | <p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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| − | <p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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| − | </div>
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| | </div> | | </div> |
| | | | |
| − | <div class="column full_size" > | + | <style> |
| − | <h5> Wiki template information </h5>
| + | .circles{ |
| − | <p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
| + | display: flex; |
| | + | margin: 0 auto; |
| | + | padding-top: 50px; |
| | + | width: 450px; |
| | + | } |
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| − | </div>
| + | .btn { |
| | + | display: block; |
| | + | width:200px; |
| | + | margin-left: 10px; |
| | + | margin-right: 10px; |
| | + | height: 200px; |
| | + | border-radius: 50%; |
| | + | border: 5px solid rgb(0,109,171);; |
| | + | } |
| | | | |
| | + | #pencil{ |
| | + | background-image: url('https://static.igem.org/mediawiki/2017/e/e3/T--UChicago--project.png'); |
| | + | opacity: .5; |
| | + | } |
| | | | |
| | + | #gavel{ |
| | + | background-image: url('https://static.igem.org/mediawiki/2017/7/77/T--UChicago--partstras.png'); |
| | + | opacity: .5; |
| | + | } |
| | | | |
| | + | #gavel:hover, #pencil:hover{ |
| | + | opacity: 1; |
| | + | } |
| | | | |
| − | <div class="column half_size" >
| + | .footer2 a{ |
| − | <h5> Editing your wiki </h5>
| + | color: white; |
| − | <p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
| + | text-decoration:none; |
| − | <p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
| + | } |
| | | | |
| − | </div>
| + | .footer2{ |
| | + | background-color: grey; |
| | + | width: auto; |
| | + | height: 50px; |
| | + | margin-top: 45px; |
| | + | text-align: center; |
| | + | color: white; |
| | + | font-family: 'Open Sans', sans-serif; |
| | + | padding-top: 28px; |
| | + | } |
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| | | | |
| − | <div class="column half_size" >
| |
| − | <h5>Tips</h5>
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| − | <p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
| |
| − | <ul>
| |
| − | <li>State your accomplishments! Tell people what you have achieved from the start. </li>
| |
| − | <li>Be clear about what you are doing and how you plan to do this.</li>
| |
| − | <li>You have a global audience! Consider the different backgrounds that your users come from.</li>
| |
| − | <li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
| |
| − | <li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
| |
| − | <li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2017.igem.org/Calendar">iGEM 2017 calendar</a> </li>
| |
| − | <li>Have lots of fun! </li>
| |
| − | </ul>
| |
| − | </div>
| |
| | | | |
| | | | |
| − | <div class="column half_size" >
| + | </style> |
| − | <h5>Inspiration</h5>
| + | <div class="footer2"> |
| − | <p> You can also view other team wikis for inspiration! Here are some examples:</p> | + | <a href="mailto:genehackers.uchicago@gmail.com">GeneHackers 2017</a> |
| − | <ul>
| + | <div> |
| − | <li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
| + | |
| − | <li> <a href=" https: //2014. igem. org/Team:Aalto-Helsinki"> 2014 Aalto-Helsinki</a > </li> | + | |
| − | <li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li> | + | |
| − | <li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
| + | |
| − | <li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
| + | |
| − | <li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
| + | |
| − | </ul>
| + | |
| − | </div>
| + | |
| | | | |
| − | <div class="column half_size" >
| |
| − | <h5> Uploading pictures and files </h5>
| |
| − | <p> You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
| |
| − | When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br>
| |
| | | | |
| − | <a href="https://2017.igem.org/Special:Upload">
| |
| − | UPLOAD FILES
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| − | </a>
| |
| − | </p>
| |
| − | </div>
| |
| | | | |
| | | | |
| | </html> | | </html> |
About GeneHackers
Genehackers is a recognized undergraduate student association with The University of Chicago. Founded in 2013, our organization aims to raise awareness about synthetic biology through community and research. We have competed in the iGem competition since the founding of our group. Our team has created innovative projects that bring synthetic biology to the center of the laboratory experience.
This year, UChicago Genehackers has set out to design the first yeast centromeric plasmid for Pichia pastoris.
iGem 2017
Our team has already made significant strides towards creating the proposed centromeric plasmid vector. We have grown psB1C3mut in LB+CAM (chloramphenicol), psD000 in LB+CAM and drop-out arginine plates and psD001 in LB+CAM. Additionally, we have amplified five potential centromeric regions in total from the chromosomal DNA of Pichia pastors.
