Difference between revisions of "Team:UChicago/Results"

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<h1>Results</h1>
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<img src="https://static.igem.org/mediawiki/2017/1/13/T--UChicago--results.png"  id="title">
  
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<p>Here you can describe the results of your project and your future plans. </p>
 
  
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<h5>What should this page contain?</h5>
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<li> Clearly and objectively describe the results of your work.</li>
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<li> Future plans for the project. </li>
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<li> Considerations for replicating the experiments. </li>
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<h5>You should also describe what your results mean: </h5>
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
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<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
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<h1>Results</h1>
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Our team has already made significant strides towards creating the proposed centromeric plasmid vector. We have transformed psB1C3mut into competent E. coli and grown the cultures in LB + CAM plates and liquid media. We similarly transformed psD000 into E. coli as well, along with <i>Pichia pastoris</i> (which we grew up in drop-out arginine plates). <br><br>
  
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<h5> Project Achievements </h5>
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Additionally, we have amplified five potential centromeric regions in total from the chromosomal DNA of <i>Pichia pastoris</i>.<br><br>
  
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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Our team has already made significant strides towards creating the proposed centromeric plasmid vector. We have grown psB1C3mut in LB+CAM (chloramphenicol), psD000 in LB+CAM and drop-out arginine plates and psD001 in LB+CAM. Additionally, we have amplified five potential centromeric regions in total from the chromosomal DNA of <i>Pichia pastoris</i>.<br><br>
  
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We PCRd yEmRFP from a plasmid in the Glick lab (pOW1-yEmRFP) and ligated it into psD000. In the future, we must sequence psD001 and attempt to grow it in yeast on -Arg plates.
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<li>A list of linked bullet points of the successful results during your project</li>
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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<h1>Future</h1>
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Although GeneHackers has made significant progress in our experiment, we still have some parts of our experiment that have not been achieved yet. We need to Gibson assembly various chromosomal sequences into psD001and perform assays of this plasmid in <i>Pichia pastoris</i>. Furthermore, if we are successful in creating the centromeric plasmid, we hope to incorporate the plasmid within the bioengineering and the industrial fields. Here, we want to test the efficiency and practicality of our plasmid within human environments. After these rounds of experimentation, we want to come back to the laboratory and improve our research with this plasmid. Hopefully, we will be able to work toward a final centromeric plasmid that will improve the course of scientific research.
 
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<h5>Inspiration</h5>
 
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<p>See how other teams presented their results.</p>
 
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
 
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
 
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
 
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<a href="mailto:genehackers.uchicago@gmail.com">GeneHackers 2017</a>
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Latest revision as of 03:31, 1 November 2017

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Results

Our team has already made significant strides towards creating the proposed centromeric plasmid vector. We have transformed psB1C3mut into competent E. coli and grown the cultures in LB + CAM plates and liquid media. We similarly transformed psD000 into E. coli as well, along with Pichia pastoris (which we grew up in drop-out arginine plates).

Additionally, we have amplified five potential centromeric regions in total from the chromosomal DNA of Pichia pastoris.

Our team has already made significant strides towards creating the proposed centromeric plasmid vector. We have grown psB1C3mut in LB+CAM (chloramphenicol), psD000 in LB+CAM and drop-out arginine plates and psD001 in LB+CAM. Additionally, we have amplified five potential centromeric regions in total from the chromosomal DNA of Pichia pastoris.

We PCRd yEmRFP from a plasmid in the Glick lab (pOW1-yEmRFP) and ligated it into psD000. In the future, we must sequence psD001 and attempt to grow it in yeast on -Arg plates.

Future

Although GeneHackers has made significant progress in our experiment, we still have some parts of our experiment that have not been achieved yet. We need to Gibson assembly various chromosomal sequences into psD001and perform assays of this plasmid in Pichia pastoris. Furthermore, if we are successful in creating the centromeric plasmid, we hope to incorporate the plasmid within the bioengineering and the industrial fields. Here, we want to test the efficiency and practicality of our plasmid within human environments. After these rounds of experimentation, we want to come back to the laboratory and improve our research with this plasmid. Hopefully, we will be able to work toward a final centromeric plasmid that will improve the course of scientific research.
GeneHackers 2017