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                        worldshaper- <b>XSHS</b>
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    <h5 class="tagline">E.coli:oxygen is delicious!</h5>
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  <h5 class="HUANGNAN">BACKGROUND</h5>
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                                        <a href="https://2017.igem.org/Team:Worldshaper-XSHS/aboutus.html">about us</a>
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                                        <a href="https://2017.igem.org/Team:Worldshaper-XSHS/Background.html">Background</a>
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                                        <a href="https://2017.igem.org/Team:Worldshaper-XSHS/design.html">Design</a>
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    <h5 class="tagline">HP</h5>
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                                        <a href="https://2017.igem.org/Team:Worldshaper-XSHS/Protocols-methods.html">Protocols and methods</a>
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                                        <a href="https://2017.igem.org/Team:Worldshaper-XSHS/result.html">Results</a>
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  <div class="copyright">&copy;2017 - <strong>IGEM worldshaperxshs</strong></div>
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                                        <a href="https://2017.igem.org/Team:Worldshaper-XSHS/Future-plans.html">Future plans</a>
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                                        <a href="https://2017.igem.org/Team:Worldshaper-XSHS/Attributions">Attributions</a>
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                                        <a href="https://2017.igem.org/Team:Worldshaper-XSHS/InterLab">Interlab</a>
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                                        <a href="https://2017.igem.org/Team:Worldshaper-XSHS/Model">Model</a>
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                                        <a href="https://2017.igem.org/Team:Worldshaper-XSHS/Demonstrate">Demonstrate</a>
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                                HUMAN PRACTICES
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                                        <a href="https://2017.igem.org/Team:Worldshaper-XSHS/HP/Silver">Silver</a>
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                                        <a href="https://2017.igem.org/Team:Worldshaper-XSHS/HP/Gold_Integrated">Integrated and Gold</a>
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                                        <a href="https://2017.igem.org/Team:Worldshaper-XSHS/Engagement">Engagement</a>
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                            <a href="https://2017.igem.org/Team:Worldshaper-XSHS/Applied_Design">Applied Design</a>
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                                        <a href="https://2017.igem.org/Team:Worldshaper-XSHS/Medal-requirements.html">Medal requirements</a>
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                            <a href="https://2017.igem.org/Team:Worldshaper-XSHS/safety.html">Safety</a>
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                        <h2>Abstract</h2>
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                        <p>
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                          Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating and low-cost tool for public to monitor water quality around.
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                            We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a GFP reporter constitute Oxygen detector 1.0. To enhance the expression of GFP, version 2.0 contains a vgb promoter, a T7 RNA polymerase gene, a T7 promoter and a GFP gene. For nitrogen, the PyeaR promoter was used to response to different concentrations of nitrate, nitrite and nitric, with a BFP reporter gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’ to sense the phosphate concentration and a RFP gene to report.
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                        <p>Worldshaper-XSHS, Xiaoshan High School</p>
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                        <p>
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                            Adress: No.538,Gongxiu Road,Xiaoshan District,Hangzhou,Zhejiang Province,China
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Latest revision as of 11:38, 1 November 2017

Abstract

Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating and low-cost tool for public to monitor water quality around.

We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a GFP reporter constitute Oxygen detector 1.0. To enhance the expression of GFP, version 2.0 contains a vgb promoter, a T7 RNA polymerase gene, a T7 promoter and a GFP gene. For nitrogen, the PyeaR promoter was used to response to different concentrations of nitrate, nitrite and nitric, with a BFP reporter gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’ to sense the phosphate concentration and a RFP gene to report.

  • Worldshaper-XSHS, Xiaoshan High School

    Adress: No.538,Gongxiu Road,Xiaoshan District,Hangzhou,Zhejiang Province,China