Difference between revisions of "Team:Worldshaper-XSHS"
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| − | + | HUMAN PRACTICES | |
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<a href="https://2017.igem.org/Team:Worldshaper-XSHS/HP/Gold_Integrated">Integrated and Gold</a> | <a href="https://2017.igem.org/Team:Worldshaper-XSHS/HP/Gold_Integrated">Integrated and Gold</a> | ||
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| + | <a href="https://2017.igem.org/Team:Worldshaper-XSHS/Engagement">Engagement</a> | ||
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<section class="visual wow fadeInLeft animated" data-wow-duration="500ms" data-wow-delay="200ms" > | <section class="visual wow fadeInLeft animated" data-wow-duration="500ms" data-wow-delay="200ms" > | ||
| − | <img src="https://static.igem.org/mediawiki/2017/d/d2/T-worldshaper-XSHS-00001.jpeg" alt="" class="bg-stretch"></section> | + | <img src="https://static.igem.org/mediawiki/2017/d/d2/T-worldshaper-XSHS-00001.jpeg" alt="" class="bg-stretch" /> |
| + | </section> | ||
<section id="about"> | <section id="about"> | ||
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| − | <h2> | + | <h2>Abstract</h2> |
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| − | + | Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating and low-cost tool for public to monitor water quality around. | |
</p> | </p> | ||
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| − | + | We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a GFP reporter constitute Oxygen detector 1.0. To enhance the expression of GFP, version 2.0 contains a vgb promoter, a T7 RNA polymerase gene, a T7 promoter and a GFP gene. For nitrogen, the PyeaR promoter was used to response to different concentrations of nitrate, nitrite and nitric, with a BFP reporter gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’ to sense the phosphate concentration and a RFP gene to report. | |
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| − | <img src="https://static.igem.org/mediawiki/2017/ | + | <img src="https://static.igem.org/mediawiki/2017/f/f1/T-worldshaper-XSHS-k010.png" alt="" /> |
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Latest revision as of 11:38, 1 November 2017
Hometown Hangzhou is widely known by water- related UNESCO World Heritages – West Lake and Grand Canal, however, severe water pollution problem caused by many reasons also exist. Hence, we hope to provide an easy-operating and low-cost tool for public to monitor water quality around.
We designed a portable water quality bio-detector prototype based on E.coli strains which were constructed to detect dissolved oxygen, phosphorus or nitrogen in water respectively. The oxygen sensitive vgb promoter and a GFP reporter constitute Oxygen detector 1.0. To enhance the expression of GFP, version 2.0 contains a vgb promoter, a T7 RNA polymerase gene, a T7 promoter and a GFP gene. For nitrogen, the PyeaR promoter was used to response to different concentrations of nitrate, nitrite and nitric, with a BFP reporter gene. For phosphate, a plasmid consisting of an ‘external phosphate sensing promoter’ to sense the phosphate concentration and a RFP gene to report.
Abstract
