Difference between revisions of "Team:NPU-China"

 
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             <div class="navbar-header">
 
             <div class="navbar-header">
 
                 <button type="button" class="navbar-toggle" data-toggle="collapse" data-target="#bs-example-navbar-collapse-1"> <span class="sr-only">Toggle navigation</span> <span class="icon-bar"></span> <span class="icon-bar"></span> <span class="icon-bar"></span> </button>
 
                 <button type="button" class="navbar-toggle" data-toggle="collapse" data-target="#bs-example-navbar-collapse-1"> <span class="sr-only">Toggle navigation</span> <span class="icon-bar"></span> <span class="icon-bar"></span> <span class="icon-bar"></span> </button>
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                 <a class="navbar-brand" href="https://2017.igem.org/Team:NPU-China">NPU-China</a>
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                 <a class="navbar-brand" href="https://2017.igem.org/Team:NPU-China">
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                  <img src="https://static.igem.org/mediawiki/2017/2/29/NPU-logo.png" style="max-width:50px;margin-top:-10px;">
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                </a>
 
             </div>
 
             </div>
 
             <div class="collapse navbar-collapse" id="bs-example-navbar-collapse-1">
 
             <div class="collapse navbar-collapse" id="bs-example-navbar-collapse-1">
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                     <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Team<b class="caret"></b></a>
 
                     <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Team<b class="caret"></b></a>
 
                         <ul class="dropdown-menu">
 
                         <ul class="dropdown-menu">
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                             <li> <a href="https://2017.igem.org/Template:NPU-China/Aboutus">About us</a> </li>
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                             <li> <a href="https://2017.igem.org/Team:NPU-China/Aboutus">About us</a> </li>
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                             <li> <a href="https://2017.igem.org/Template:NPU-China/Attribution">Attribution</a> </li>
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                             <li> <a href="https://2017.igem.org/Team:NPU-China/Attributions">Attributions</a> </li>
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
 
                     <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Project<b class="caret"></b></a>
 
                     <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Project<b class="caret"></b></a>
 
                         <ul class="dropdown-menu">
 
                         <ul class="dropdown-menu">
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                             <li> <a href="https://2017.igem.org/Template:NPU-China/Background">Background</a> </li>
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                             <li> <a href="https://2017.igem.org/Team:NPU-China/Background">Background</a> </li>
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                             <li> <a href="https://2017.igem.org/Template:NPU-China/Description">Description</a> </li>
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                             <li> <a href="https://2017.igem.org/Team:NPU-China/Description">Description</a> </li>
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                             <li> <a href="https://2017.igem.org/Template:NPU-China/Design">Design</a> </li>
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                             <li> <a href="https://2017.igem.org/Team:NPU-China/Design">Design</a> </li>
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                             <li> <a href="https://2017.igem.org/Template:NPU-China/Model">Model</a> </li>
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                             <li> <a href="https://2017.igem.org/Team:NPU-China/Model">Model</a> </li>
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                             <li> <a href="https://2017.igem.org/Template:NPU-China/Proofofconcept">Proof of concept</a> </li>
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                             <li> <a href="https://2017.igem.org/Team:NPU-China/Proofofconcept">Proof of concept</a> </li>
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                             <li> <a href="https://2017.igem.org/Template:NPU-China/Demonstrate">Demonstrate</a> </li>
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                             <li> <a href="https://2017.igem.org/Team:NPU-China/Demonstrate">Demonstrate</a> </li>
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
 
                     <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Parts<b class="caret"></b></a>
 
                     <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Parts<b class="caret"></b></a>
 
                         <ul class="dropdown-menu">
 
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                             <li> <a href="https://2017.igem.org/Template:NPU-China/BasicParts">Basic Parts</a> </li>
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                             <li> <a href="https://2017.igem.org/Team:NPU-China/BasicParts">Basic Parts</a> </li>
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                             <li> <a href="https://2017.igem.org/Template:NPU-China/CompositeParts">Composite Parts</a> </li>
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                             <li> <a href="https://2017.igem.org/Team:NPU-China/CompositeParts">Composite Parts</a> </li>
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
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                     <li> <a href="https://2017.igem.org/Template:NPU-China/Hardware">Hardware</a> </li>
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                     <li> <a href="https://2017.igem.org/Team:NPU-China/Hardware">Hardware</a> </li>
 
                     <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">HP<b class="caret"></b></a>
 
                     <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">HP<b class="caret"></b></a>
 
                         <ul class="dropdown-menu">
 
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                             <li> <a href="https://2017.igem.org/Template:NPU-China/Silver">Silver</a> </li>
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                             <li> <a href="https://2017.igem.org/Team:NPU-China/HP/Silver">Silver</a> </li>
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                             <li> <a href="https://2017.igem.org/Template:NPU-China/Gold">Gold</a> </li>
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                             <li> <a href="https://2017.igem.org/Team:NPU-China/HP/Gold_Integrated">Gold</a> </li>
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
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                     <li> <a href="https://2017.igem.org/Template:NPU-China/Collaboration">Collaboration</a> </li>
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                     <li> <a href="https://2017.igem.org/Team:NPU-China/Collaborations">Collaborations</a> </li>
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                     <li> <a href="https://2017.igem.org/Template:NPU-China/Achievements">Achievements</a> </li>
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                     <li> <a href="https://2017.igem.org/Team:NPU-China/Achievements">Achievements</a> </li>
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                     <li> <a href="https://2017.igem.org/Template:NPU-China/Interlab">Interlab</a> </li>
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                     <li> <a href="https://2017.igem.org/Team:NPU-China/InterLab">InterLab</a> </li>
  
 
                     <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook<b class="caret"></b></a>
 
                     <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook<b class="caret"></b></a>
 
                         <ul class="dropdown-menu">
 
                         <ul class="dropdown-menu">
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                             <li> <a href="https://2017.igem.org/Template:NPU-China/Labnotes">Labnotes</a> </li>
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                             <li> <a href="https://2017.igem.org/Team:NPU-China/Labnotes">Labnotes</a> </li>
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                             <li> <a href="https://2017.igem.org/Template:NPU-China/Protocols">Protocols</a> </li>
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                             <li> <a href="https://2017.igem.org/Team:NPU-China/Protocols">Protocols</a> </li>
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
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             </div>
 
             </div>
 
             <div class="item">
 
             <div class="item">
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                <img src="https://static.igem.org/mediawiki/2017/2/28/Npu-banner2.jpg">
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                <img src="https://static.igem.org/mediawiki/2017/2/28/Npu-banner2.jpg">
 
             </div>
 
             </div>
 
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         <!-- Controls -->
 
         <!-- Controls -->
 
     </header>
 
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     <div class="batu" style="background: url('https://static.igem.org/mediawiki/2017/f/fe/Npu-background.png') no-repeat fixed;">
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     <div class="batu" style="background: url('https://static.igem.org/mediawiki/2017/f/fe/Npu-background.png') no-repeat fixed; overflow: hidden;">
 
         <!-- Page Content -->
 
         <!-- Page Content -->
 
         <div class="container">
 
         <div class="container">
  
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                 <div class="col-md-12">
 
                 <div class="col-md-12">
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                     <h2 class="page-header">Abstract</h2>
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                     <h2 class="page-header" align="center" >Abstract</h2>
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                     <h3>Acrylic acid is a bulk chemical raw material, which is widely used in many fields because of its excellent
+
                     <h4>Acrylic acid is a bulk chemical raw material, which is widely used in many fields because of its excellent
 
                         polymerization capacity, such as paint, glue, and even mobile phone screen protective film. The average
 
                         polymerization capacity, such as paint, glue, and even mobile phone screen protective film. The average
 
                         annual market demand of acrylic acid is up to 8 million tons, and the market value is nearly 10 billion
 
                         annual market demand of acrylic acid is up to 8 million tons, and the market value is nearly 10 billion
−
                         US dollars. It has broad market prospect. At present, acrylic acid is made from propylene (which
+
                         US dollars, which shows a promising market prospect. At present, acrylic acid is made from propylene (which
−
                         is obtained by petroleum cracking) after multi-step treatment. The production process causes pollution,
+
                         is obtained by petroleum cracking) after multi-step treatment, resulting in environmental pollution,
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                         high energy consumption and it is unsustainable.<br> This year, we aim to use a green and environmentally
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                         high energy consumption and a lack of sustainablility.<br><br> This year, we aim to use a greener and more environmentally-
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                         friendly carbon source, glycerol to achieve all green production of acrylic acid. Compared to traditional
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                         friendly carbon source, glycerol, to achieve all green production of acrylic acid. Compared to traditional
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                         chemical synthesis methods, Synbio is green and sustainable, and glycerol is cheaper than ethylene.
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                         chemical synthesis methods, Synbio is relatively greener and more sustainable. Also, glycerol costs less than propene.
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                     </h3>
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                     </h4>
 +
                 
 
                 </div>
 
                 </div>
 
             </div>
 
             </div>
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             <!-- Marketing Icons Section -->
 
             <!-- Marketing Icons Section -->
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                 <div class="col-md-12">
 
                 <div class="col-md-12">
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                     <h2 class="page-header">Some fk</h2>
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                     <h2 class="page-header" align="center">We constructed our cell factory based on 4 levels, which are—</h2>
 +
                    <br>
 
                 </div>
 
                 </div>
 
             </div>
 
             </div>
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             <div class="row">
 
             <div class="row">
 
                 <div class="col-md-6 img-portfolio">
 
                 <div class="col-md-6 img-portfolio">
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                     <a href="portfolio-item.html">
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                     <a href="https://2017.igem.org/Team:NPU-China/Design#COREPART">
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                         <img class="img-responsive img-hover" src="http://placehold.it/700x400" alt="">
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                         <img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/a/ac/Ceas2.png">
 
                     </a>
 
                     </a>
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                     <h3>
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                     <h3 align="center">
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                         <a href="portfolio-item.html">Project One</a>
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                         <a href="https://2017.igem.org/Team:NPU-China/Design#COREPART">Core Part</a>
 
                     </h3>
 
                     </h3>
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                     <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nam viverra euismod odio, gravida pellentesque
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                     <h4>We use ceaS2 enzyme as the core part, but acrylic acid is a by-product of ceaS2 enzyme, whose catalytic effect of wild type
−
                         urna varius vitae.</p>
+
                        is very weak with acrylic acid production only 1mg/L. Hence, we hope to improve the catalytic
 +
                         effect of ceaS2 enzyme.<br><br> We designed ceaS2 enzyme mutants via the AEMD(Auto Enzyme Mutation Design)
 +
                        platform and screened for ceaS2 mutants that own better acrylic acid yield by HPLC(High Performance Liquid Chromatography)
 +
                        and HTS(High throughput screening).
 +
                    </h4>
 
                 </div>
 
                 </div>
 
                 <div class="col-md-6 img-portfolio">
 
                 <div class="col-md-6 img-portfolio">
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                     <a href="portfolio-item.html">
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                     <a href="https://2017.igem.org/Team:NPU-China/Design#SYSTEM">
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                         <img class="img-responsive img-hover" src="http://placehold.it/700x400" alt="">
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                         <img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/8/85/System.png" alt="">
 
                     </a>
 
                     </a>
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                     <h3>
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                     <h3 align="center">
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                         <a href="portfolio-item.html">Project Two</a>
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                         <a href="https://2017.igem.org/Team:NPU-China/Design#SYSTEM">System</a>
 
                     </h3>
 
                     </h3>
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                     <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nam viverra euismod odio, gravida pellentesque
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                     <h4>Respectively, E. coli and S. cerevisiae are the two sorts of model organisms that are most convenient
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                         urna varius vitae.</p>
+
                        to operate in the prokaryote and eukaryote. Therefore, in terms of our choice of the chassis organisms,
 +
                         we have E. coli MG1655 and S. cerevisiae BY4741 tested individually.
 +
                    </h4>
 
                 </div>
 
                 </div>
 
             </div>
 
             </div>
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             <!-- Projects Row -->
 
             <!-- Projects Row -->
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             <div class="row">
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             <div class="row"  style="padding-top:50px">
 
                 <div class="col-md-6 img-portfolio">
 
                 <div class="col-md-6 img-portfolio">
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                     <a href="portfolio-item.html">
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                     <a href="https://2017.igem.org/Team:NPU-China/Design#PATHWAY">
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                         <img class="img-responsive img-hover" src="http://placehold.it/700x400" alt="">
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                         <img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/e/ec/Pathway.png" alt="">
 
                     </a>
 
                     </a>
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                     <h3>
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                     <h3 align="center">
−
                         <a href="portfolio-item.html">Project Three</a>
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                         <a href="https://2017.igem.org/Team:NPU-China/Design#PATHWAY">Pathway</a>
 
                     </h3>
 
                     </h3>
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                     <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nam viverra euismod odio, gravida pellentesque
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                     <h4>We need to design two different metabolic pathways for two different chassis organisms and propose different
−
                         urna varius vitae.</p>
+
                        optimization schemes. We introduced the ceaS2 enzyme exogenously on the basis of the glycerol
 +
                        metabolism of the two organisms, so that they could produce the target product acrylic acid using the
 +
                        intermediates G3P and DHAP.<br><br> Besides the construction of the pathways, we also reconstructed
 +
                        and optimized the original metabolic pathway to increase the carbon flux rate of the designed pathway
 +
                         and reduced the loss of by-pass carbon flux.</h4>
 
                 </div>
 
                 </div>
 
                 <div class="col-md-6 img-portfolio">
 
                 <div class="col-md-6 img-portfolio">
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                     <a href="portfolio-item.html">
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                     <a href="https://2017.igem.org/Team:NPU-China/Design#PRODUCTION">
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                         <img class="img-responsive img-hover" src="http://placehold.it/700x400" alt="">
+
                         <img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/6/67/Production2.png" alt="">
 
                     </a>
 
                     </a>
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                     <h3>
+
                     <h3 align="center">
−
                         <a href="portfolio-item.html">Project Four</a>
+
                         <a href="https://2017.igem.org/Team:NPU-China/Design#PRODUCTION" >Production</a>
 
                     </h3>
 
                     </h3>
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                     <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nam viverra euismod odio, gravida pellentesque
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                     <h4>All of the previous processes were applied in building the engineered microorganism strains which have a high production of acrylic
−
                         urna varius vitae.</p>
+
                        acid. In the subsequent fermentation, we also determined the best conditions
 +
                        of the engineered microorganism strains.<br><br> Therefore, we selected to control the carbon source, buffer, temperature, pH
 +
                         and other conditions to optimize the cell production process.</h4>
 
                 </div>
 
                 </div>
 
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             </div>
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        <img src="https://static.igem.org/mediawiki/2017/0/0c/Jz.png" class="img-responsive">
  
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            <!-- Footer -->
 
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            <footer>
 
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                <div class="row">
 
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                    <div class="col-md-12">
 
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                        <p>Copyright &copy; NPU-CHINA 2017</p>
 
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Latest revision as of 12:52, 1 November 2017

Abstract

Acrylic acid is a bulk chemical raw material, which is widely used in many fields because of its excellent polymerization capacity, such as paint, glue, and even mobile phone screen protective film. The average annual market demand of acrylic acid is up to 8 million tons, and the market value is nearly 10 billion US dollars, which shows a promising market prospect. At present, acrylic acid is made from propylene (which is obtained by petroleum cracking) after multi-step treatment, resulting in environmental pollution, high energy consumption and a lack of sustainablility.

This year, we aim to use a greener and more environmentally- friendly carbon source, glycerol, to achieve all green production of acrylic acid. Compared to traditional chemical synthesis methods, Synbio is relatively greener and more sustainable. Also, glycerol costs less than propene.

We constructed our cell factory based on 4 levels, which are—


Core Part

We use ceaS2 enzyme as the core part, but acrylic acid is a by-product of ceaS2 enzyme, whose catalytic effect of wild type is very weak with acrylic acid production only 1mg/L. Hence, we hope to improve the catalytic effect of ceaS2 enzyme.

We designed ceaS2 enzyme mutants via the AEMD(Auto Enzyme Mutation Design) platform and screened for ceaS2 mutants that own better acrylic acid yield by HPLC(High Performance Liquid Chromatography) and HTS(High throughput screening).

System

Respectively, E. coli and S. cerevisiae are the two sorts of model organisms that are most convenient to operate in the prokaryote and eukaryote. Therefore, in terms of our choice of the chassis organisms, we have E. coli MG1655 and S. cerevisiae BY4741 tested individually.

Pathway

We need to design two different metabolic pathways for two different chassis organisms and propose different optimization schemes. We introduced the ceaS2 enzyme exogenously on the basis of the glycerol metabolism of the two organisms, so that they could produce the target product acrylic acid using the intermediates G3P and DHAP.

Besides the construction of the pathways, we also reconstructed and optimized the original metabolic pathway to increase the carbon flux rate of the designed pathway and reduced the loss of by-pass carbon flux.

Production

All of the previous processes were applied in building the engineered microorganism strains which have a high production of acrylic acid. In the subsequent fermentation, we also determined the best conditions of the engineered microorganism strains.

Therefore, we selected to control the carbon source, buffer, temperature, pH and other conditions to optimize the cell production process.