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<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
<li> <a href="https://2017.igem.org/Team:NPU-China/Aboutus">About us</a> </li> | <li> <a href="https://2017.igem.org/Team:NPU-China/Aboutus">About us</a> </li> | ||
− | <li> <a href="https://2017.igem.org/Team:NPU-China/ | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Attributions">Attributions</a> </li> |
</ul> | </ul> | ||
</li> | </li> | ||
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<li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">HP<b class="caret"></b></a> | <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">HP<b class="caret"></b></a> | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
− | <li> <a href="https://2017.igem.org/Team:NPU-China/Silver">Silver</a> </li> | + | <li> <a href="https://2017.igem.org/Team:NPU-China/HP/Silver">Silver</a> </li> |
− | <li> <a href="https://2017.igem.org/Team:NPU-China/ | + | <li> <a href="https://2017.igem.org/Team:NPU-China/HP/Gold_Integrated">Gold</a> </li> |
</ul> | </ul> | ||
</li> | </li> | ||
− | <li> <a href="https://2017.igem.org/Team:NPU-China/ | + | <li> <a href="https://2017.igem.org/Team:NPU-China/Collaborations">Collaborations</a> </li> |
<li> <a href="https://2017.igem.org/Team:NPU-China/Achievements">Achievements</a> </li> | <li> <a href="https://2017.igem.org/Team:NPU-China/Achievements">Achievements</a> </li> | ||
− | <li> <a href="https://2017.igem.org/Team:NPU-China/ | + | <li> <a href="https://2017.igem.org/Team:NPU-China/InterLab">InterLab</a> </li> |
<li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook<b class="caret"></b></a> | <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook<b class="caret"></b></a> | ||
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<div class="container"> | <div class="container"> | ||
− | <div class="row"> | + | <div class="row" style=" padding-top:70px"> |
<div class="col-md-12"> | <div class="col-md-12"> | ||
− | <h2 class="page-header">Abstract</h2> | + | <h2 class="page-header" align="center" >Abstract</h2> |
− | < | + | <h4>Acrylic acid is a bulk chemical raw material, which is widely used in many fields because of its excellent |
polymerization capacity, such as paint, glue, and even mobile phone screen protective film. The average | polymerization capacity, such as paint, glue, and even mobile phone screen protective film. The average | ||
annual market demand of acrylic acid is up to 8 million tons, and the market value is nearly 10 billion | annual market demand of acrylic acid is up to 8 million tons, and the market value is nearly 10 billion | ||
− | US dollars | + | US dollars, which shows a promising market prospect. At present, acrylic acid is made from propylene (which |
− | is obtained by petroleum cracking) after multi-step treatment | + | is obtained by petroleum cracking) after multi-step treatment, resulting in environmental pollution, |
− | high energy consumption and | + | high energy consumption and a lack of sustainablility.<br><br> This year, we aim to use a greener and more environmentally- |
− | friendly carbon source, glycerol to achieve all green production of acrylic acid. Compared to traditional | + | friendly carbon source, glycerol, to achieve all green production of acrylic acid. Compared to traditional |
− | chemical synthesis methods, Synbio is | + | chemical synthesis methods, Synbio is relatively greener and more sustainable. Also, glycerol costs less than propene. |
− | </ | + | </h4> |
+ | |||
</div> | </div> | ||
</div> | </div> | ||
− | + | ||
<!-- Marketing Icons Section --> | <!-- Marketing Icons Section --> | ||
− | <div class="row"> | + | <div class="row" style="padding-top:70px"> |
<div class="col-md-12"> | <div class="col-md-12"> | ||
− | <h2 class="page-header">We | + | <h2 class="page-header" align="center">We constructed our cell factory based on 4 levels, which are—</h2> |
<br> | <br> | ||
</div> | </div> | ||
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<div class="row"> | <div class="row"> | ||
<div class="col-md-6 img-portfolio"> | <div class="col-md-6 img-portfolio"> | ||
− | <a href=" | + | <a href="https://2017.igem.org/Team:NPU-China/Design#COREPART"> |
<img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/a/ac/Ceas2.png"> | <img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/a/ac/Ceas2.png"> | ||
</a> | </a> | ||
− | <h3> | + | <h3 align="center"> |
− | <a href=" | + | <a href="https://2017.igem.org/Team:NPU-China/Design#COREPART">Core Part</a> |
</h3> | </h3> | ||
− | <h4>We use ceaS2 enzyme as the core part, but acrylic acid is a | + | <h4>We use ceaS2 enzyme as the core part, but acrylic acid is a by-product of ceaS2 enzyme, whose catalytic effect of wild type |
− | + | is very weak with acrylic acid production only 1mg/L. Hence, we hope to improve the catalytic | |
− | effect of ceaS2 enzyme.<br> We designed ceaS2 enzyme mutants via the AEMD(Auto Enzyme Mutation Design) | + | effect of ceaS2 enzyme.<br><br> We designed ceaS2 enzyme mutants via the AEMD(Auto Enzyme Mutation Design) |
− | platform and screened for | + | platform and screened for ceaS2 mutants that own better acrylic acid yield by HPLC(High Performance Liquid Chromatography) |
and HTS(High throughput screening). | and HTS(High throughput screening). | ||
</h4> | </h4> | ||
</div> | </div> | ||
<div class="col-md-6 img-portfolio"> | <div class="col-md-6 img-portfolio"> | ||
− | <a href=" | + | <a href="https://2017.igem.org/Team:NPU-China/Design#SYSTEM"> |
<img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/8/85/System.png" alt=""> | <img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/8/85/System.png" alt=""> | ||
</a> | </a> | ||
− | <h3> | + | <h3 align="center"> |
− | <a href=" | + | <a href="https://2017.igem.org/Team:NPU-China/Design#SYSTEM">System</a> |
</h3> | </h3> | ||
<h4>Respectively, E. coli and S. cerevisiae are the two sorts of model organisms that are most convenient | <h4>Respectively, E. coli and S. cerevisiae are the two sorts of model organisms that are most convenient | ||
to operate in the prokaryote and eukaryote. Therefore, in terms of our choice of the chassis organisms, | to operate in the prokaryote and eukaryote. Therefore, in terms of our choice of the chassis organisms, | ||
− | we have | + | we have E. coli MG1655 and S. cerevisiae BY4741 tested individually. |
</h4> | </h4> | ||
</div> | </div> | ||
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<!-- Projects Row --> | <!-- Projects Row --> | ||
− | <div class="row"> | + | <div class="row" style="padding-top:50px"> |
<div class="col-md-6 img-portfolio"> | <div class="col-md-6 img-portfolio"> | ||
− | <a href=" | + | <a href="https://2017.igem.org/Team:NPU-China/Design#PATHWAY"> |
<img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/e/ec/Pathway.png" alt=""> | <img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/e/ec/Pathway.png" alt=""> | ||
</a> | </a> | ||
− | <h3> | + | <h3 align="center"> |
− | <a href=" | + | <a href="https://2017.igem.org/Team:NPU-China/Design#PATHWAY">Pathway</a> |
</h3> | </h3> | ||
<h4>We need to design two different metabolic pathways for two different chassis organisms and propose different | <h4>We need to design two different metabolic pathways for two different chassis organisms and propose different | ||
− | optimization schemes | + | optimization schemes. We introduced the ceaS2 enzyme exogenously on the basis of the glycerol |
− | metabolism of the two | + | metabolism of the two organisms, so that they could produce the target product acrylic acid using the |
− | intermediates G3P and DHAP.Besides | + | intermediates G3P and DHAP.<br><br> Besides the construction of the pathways, we also reconstructed |
and optimized the original metabolic pathway to increase the carbon flux rate of the designed pathway | and optimized the original metabolic pathway to increase the carbon flux rate of the designed pathway | ||
− | and | + | and reduced the loss of by-pass carbon flux.</h4> |
</div> | </div> | ||
<div class="col-md-6 img-portfolio"> | <div class="col-md-6 img-portfolio"> | ||
− | <a href=" | + | <a href="https://2017.igem.org/Team:NPU-China/Design#PRODUCTION"> |
− | <img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/ | + | <img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/6/67/Production2.png" alt=""> |
</a> | </a> | ||
− | <h3> | + | <h3 align="center"> |
− | <a href=" | + | <a href="https://2017.igem.org/Team:NPU-China/Design#PRODUCTION" >Production</a> |
</h3> | </h3> | ||
− | <h4>All of the previous processes | + | <h4>All of the previous processes were applied in building the engineered microorganism strains which have a high production of acrylic |
− | acid | + | acid. In the subsequent fermentation, we also determined the best conditions |
− | of the | + | of the engineered microorganism strains.<br><br> Therefore, we selected to control the carbon source, buffer, temperature, pH |
and other conditions to optimize the cell production process.</h4> | and other conditions to optimize the cell production process.</h4> | ||
</div> | </div> | ||
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</div> | </div> | ||
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− | <img src="https://static.igem.org/mediawiki/2017/0/0c/Jz.png"> | + | <img src="https://static.igem.org/mediawiki/2017/0/0c/Jz.png" class="img-responsive"> |
</div> | </div> |
Latest revision as of 12:52, 1 November 2017