Difference between revisions of "Team:NPU-China"

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{{NPU-China}}
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{{NPU-China/mmp}}
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<html>
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<html lang="en">
  
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<head>
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    <!-- Bootstrap Core CSS -->
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    <link href="https://cdn.bootcss.com/bootstrap/3.3.7/css/bootstrap.min.css" rel="stylesheet">
  
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<div class="column full_size" >
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    <!-- Custom CSS -->
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<img src="http://placehold.it/2000x300/d3d3d3/f2f2f2">
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    <link href="https://2017.igem.org/Template:NPU-China/css?action=raw&ctype=text/css" rel="stylesheet">
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</div>
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 +
    <!-- Custom Fonts -->
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    <link href="https://cdn.bootcss.com/font-awesome/4.7.0/css/font-awesome.min.css" rel="stylesheet">
  
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    <!-- jQuery -->
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    <script src="https://cdn.bootcss.com/jquery/3.2.1/jquery.min.js"></script>
  
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<div class="column full_size" >
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    <!-- Bootstrap Core JavaScript -->
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<h1> Welcome to iGEM 2017! </h1>
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    <script src="https://cdn.bootcss.com/bootstrap/3.3.7/js/bootstrap.min.js"></script>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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</div>  
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<div class="clear"></div>
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    <!-- HTML5 Shim and Respond.js IE8 support of HTML5 elements and media queries -->
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    <!-- WARNING: Respond.js doesn't work if you view the page via file:// -->
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    <!--[if lt IE 9]>
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        <script src="https://oss.maxcdn.com/libs/html5shiv/3.7.0/html5shiv.js"></script>
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        <script src="https://oss.maxcdn.com/libs/respond.js/1.4.2/respond.min.js"></script>
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    <![endif]-->
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    <style>
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    </style>
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</head>
  
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<div class="column half_size" >
 
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<h5>Before you start: </h5>
 
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<p> Please read the following pages:</p>
 
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<ul>
 
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<li>  <a href="https://2017.igem.org/Competition">Competition Hub</a> </li>
 
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<li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
 
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<li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
 
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</ul>
 
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</div>
 
  
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<div class="column half_size" >
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<body>
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<div class="highlight">
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<h5> Styling your wiki </h5>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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</div>
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</div>
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<div class="column full_size" >
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    <!-- Navigation -->
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<h5> Wiki template information </h5>
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    <nav class="navbar navbar-inverse navbar-fixed-top" role="navigation">
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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        <div class="container">
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            <div class="navbar-header">
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                <button type="button" class="navbar-toggle" data-toggle="collapse" data-target="#bs-example-navbar-collapse-1"> <span class="sr-only">Toggle navigation</span> <span class="icon-bar"></span> <span class="icon-bar"></span> <span class="icon-bar"></span> </button>
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                <a class="navbar-brand" href="https://2017.igem.org/Team:NPU-China">
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                  <img src="https://static.igem.org/mediawiki/2017/2/29/NPU-logo.png" style="max-width:50px;margin-top:-10px;">
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                </a>
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            </div>
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            <div class="collapse navbar-collapse" id="bs-example-navbar-collapse-1">
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                <ul class="nav navbar-nav navbar-right">
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                    <li class="active"> <a href="https://2017.igem.org/Team:NPU-China">Home</a> </li>
 +
                    <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Team<b class="caret"></b></a>
 +
                        <ul class="dropdown-menu">
 +
                            <li> <a href="https://2017.igem.org/Team:NPU-China/Aboutus">About us</a> </li>
 +
                            <li> <a href="https://2017.igem.org/Team:NPU-China/Attributions">Attributions</a> </li>
 +
                        </ul>
 +
                    </li>
 +
                    <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Project<b class="caret"></b></a>
 +
                        <ul class="dropdown-menu">
 +
                            <li> <a href="https://2017.igem.org/Team:NPU-China/Background">Background</a> </li>
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                            <li> <a href="https://2017.igem.org/Team:NPU-China/Description">Description</a> </li>
 +
                            <li> <a href="https://2017.igem.org/Team:NPU-China/Design">Design</a> </li>
 +
                            <li> <a href="https://2017.igem.org/Team:NPU-China/Model">Model</a> </li>
 +
                            <li> <a href="https://2017.igem.org/Team:NPU-China/Proofofconcept">Proof of concept</a> </li>
 +
                            <li> <a href="https://2017.igem.org/Team:NPU-China/Demonstrate">Demonstrate</a> </li>
 +
                        </ul>
 +
                    </li>
 +
                    <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Parts<b class="caret"></b></a>
 +
                        <ul class="dropdown-menu">
 +
                            <li> <a href="https://2017.igem.org/Team:NPU-China/BasicParts">Basic Parts</a> </li>
 +
                            <li> <a href="https://2017.igem.org/Team:NPU-China/CompositeParts">Composite Parts</a> </li>
 +
                        </ul>
 +
                    </li>
 +
                    <li> <a href="https://2017.igem.org/Team:NPU-China/Hardware">Hardware</a> </li>
 +
                    <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">HP<b class="caret"></b></a>
 +
                        <ul class="dropdown-menu">
 +
                            <li> <a href="https://2017.igem.org/Team:NPU-China/HP/Silver">Silver</a> </li>
 +
                            <li> <a href="https://2017.igem.org/Team:NPU-China/HP/Gold_Integrated">Gold</a> </li>
 +
                        </ul>
 +
                    </li>
 +
                    <li> <a href="https://2017.igem.org/Team:NPU-China/Collaborations">Collaborations</a> </li>
 +
                    <li> <a href="https://2017.igem.org/Team:NPU-China/Achievements">Achievements</a> </li>
 +
                    <li> <a href="https://2017.igem.org/Team:NPU-China/InterLab">InterLab</a> </li>
  
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</div>  
+
                    <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook<b class="caret"></b></a>
 +
                        <ul class="dropdown-menu">
 +
                            <li> <a href="https://2017.igem.org/Team:NPU-China/Labnotes">Labnotes</a> </li>
 +
                            <li> <a href="https://2017.igem.org/Team:NPU-China/Protocols">Protocols</a> </li>
 +
                        </ul>
 +
                    </li>
 +
                </ul>
 +
            </div>
 +
        </div>
 +
    </nav>
  
 +
    <!-- Header Carousel -->
 +
    <header id="myCarousel" class="carousel slide">
 +
        <!-- Indicators -->
 +
        <div class="carousel-indicators">
 +
            <li data-target="#myCarousel" data-slide-to="0" class="active"></li>
 +
            <li data-target="#myCarousel" data-slide-to="1"></li>
 +
            <li data-target="#myCarousel" data-slide-to="2"></li>
 +
        </div>
  
 +
        <!-- Wrapper for slides -->
 +
        <div class="carousel-inner">
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            <div class="item active">
 +
                <img src="https://static.igem.org/mediawiki/2017/1/1f/Npu-banner1.jpg">
  
 +
            </div>
 +
            <div class="item">
 +
                <img src="https://static.igem.org/mediawiki/2017/0/06/Npu-banner3.jpg">
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            </div>
 +
            <div class="item">
 +
                <img src="https://static.igem.org/mediawiki/2017/2/28/Npu-banner2.jpg">
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            </div>
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        </div>
 +
        <a class="left carousel-control" href="#myCarousel" data-slide="prev">
 +
            <span class="icon-prev"></span>
 +
        </a>
 +
        <a class="right carousel-control" href="#myCarousel" data-slide="next">
 +
            <span class="icon-next"></span>
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        </a>
 +
        <!-- Controls -->
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    </header>
  
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<div class="column half_size" >
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    <div class="batu" style="background: url('https://static.igem.org/mediawiki/2017/f/fe/Npu-background.png') no-repeat fixed; overflow: hidden;">
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<h5> Editing your wiki </h5>
+
        <!-- Page Content -->
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
+
        <div class="container">
−
<p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
+
  
−
</div>
+
            <div class="row" style=" padding-top:70px">
 +
                <div class="col-md-12">
 +
                    <h2 class="page-header" align="center" >Abstract</h2>
 +
                    <h4>Acrylic acid is a bulk chemical raw material, which is widely used in many fields because of its excellent
 +
                        polymerization capacity, such as paint, glue, and even mobile phone screen protective film. The average
 +
                        annual market demand of acrylic acid is up to 8 million tons, and the market value is nearly 10 billion
 +
                        US dollars, which shows a promising market prospect. At present, acrylic acid is made from propylene (which
 +
                        is obtained by petroleum cracking) after multi-step treatment, resulting in environmental pollution,
 +
                        high energy consumption and a lack of sustainablility.<br><br> This year, we aim to use a greener and more environmentally-
 +
                        friendly carbon source, glycerol, to achieve all green production of acrylic acid. Compared to traditional
 +
                        chemical synthesis methods, Synbio is relatively greener and more sustainable. Also, glycerol costs less than propene.
 +
                    </h4>
 +
                 
 +
                </div>
 +
            </div>
 +
       
 +
            <!-- Marketing Icons Section -->
 +
            <div class="row" style="padding-top:70px">
 +
                <div class="col-md-12">
 +
                    <h2 class="page-header" align="center">We constructed our cell factory based on 4 levels, which are—</h2>
 +
                    <br>
 +
                </div>
 +
            </div>
  
  
−
<div class="column half_size" >
 
−
<h5>Tips</h5>
 
−
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
 
−
<ul>
 
−
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
 
−
<li>Be clear about what you are doing and how you plan to do this.</li>
 
−
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
 
−
<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
 
−
<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
 
−
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2017.igem.org/Calendar">iGEM 2017 calendar</a> </li>
 
−
<li>Have lots of fun! </li>
 
−
</ul>
 
−
</div>
 
  
  
−
<div class="column half_size" >
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            <div class="row">
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<h5>Inspiration</h5>
+
                <div class="col-md-6 img-portfolio">
−
<p> You can also view other team wikis for inspiration! Here are some examples:</p>
+
                    <a href="https://2017.igem.org/Team:NPU-China/Design#COREPART">
−
<ul>
+
                        <img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/a/ac/Ceas2.png">
−
<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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                    </a>
−
<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
+
                    <h3 align="center">
−
<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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                        <a href="https://2017.igem.org/Team:NPU-China/Design#COREPART">Core Part</a>
−
<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
+
                    </h3>
−
<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
+
                    <h4>We use ceaS2 enzyme as the core part, but acrylic acid is a by-product of ceaS2 enzyme, whose catalytic effect of wild type
−
<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
+
                        is very weak with acrylic acid production only 1mg/L. Hence, we hope to improve the catalytic
−
</ul>
+
                        effect of ceaS2 enzyme.<br><br> We designed ceaS2 enzyme mutants via the AEMD(Auto Enzyme Mutation Design)
−
</div>
+
                        platform and screened for ceaS2 mutants that own better acrylic acid yield by HPLC(High Performance Liquid Chromatography)
−
 
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                        and HTS(High throughput screening).
−
<div class="column half_size" >
+
                    </h4>
−
<h5> Uploading pictures and files </h5>
+
                </div>
−
<p> You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
+
                <div class="col-md-6 img-portfolio">
−
When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br>
+
                    <a href="https://2017.igem.org/Team:NPU-China/Design#SYSTEM">
−
 
+
                        <img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/8/85/System.png" alt="">
−
<a href="https://2017.igem.org/Special:Upload">
+
                    </a>
−
UPLOAD FILES
+
                    <h3 align="center">
−
</a>
+
                        <a href="https://2017.igem.org/Team:NPU-China/Design#SYSTEM">System</a>
−
</p>
+
                    </h3>
−
</div>
+
                    <h4>Respectively, E. coli and S. cerevisiae are the two sorts of model organisms that are most convenient
 +
                        to operate in the prokaryote and eukaryote. Therefore, in terms of our choice of the chassis organisms,
 +
                        we have E. coli MG1655 and S. cerevisiae BY4741 tested individually.
 +
                    </h4>
 +
                </div>
 +
            </div>
 +
            <!-- /.row -->
  
 +
            <!-- Projects Row -->
 +
            <div class="row"  style="padding-top:50px">
 +
                <div class="col-md-6 img-portfolio">
 +
                    <a href="https://2017.igem.org/Team:NPU-China/Design#PATHWAY">
 +
                        <img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/e/ec/Pathway.png" alt="">
 +
                    </a>
 +
                    <h3 align="center">
 +
                        <a href="https://2017.igem.org/Team:NPU-China/Design#PATHWAY">Pathway</a>
 +
                    </h3>
 +
                    <h4>We need to design two different metabolic pathways for two different chassis organisms and propose different
 +
                        optimization schemes. We introduced the ceaS2 enzyme exogenously on the basis of the glycerol
 +
                        metabolism of the two organisms, so that they could produce the target product acrylic acid using the
 +
                        intermediates G3P and DHAP.<br><br> Besides the construction of the pathways, we also reconstructed
 +
                        and optimized the original metabolic pathway to increase the carbon flux rate of the designed pathway
 +
                        and reduced the loss of by-pass carbon flux.</h4>
 +
                </div>
 +
                <div class="col-md-6 img-portfolio">
 +
                    <a href="https://2017.igem.org/Team:NPU-China/Design#PRODUCTION">
 +
                        <img class="img-responsive img-hover" src="https://static.igem.org/mediawiki/2017/6/67/Production2.png" alt="">
 +
                    </a>
 +
                    <h3 align="center">
 +
                        <a href="https://2017.igem.org/Team:NPU-China/Design#PRODUCTION" >Production</a>
 +
                    </h3>
 +
                    <h4>All of the previous processes were applied in building the engineered microorganism strains which have a high production of acrylic
 +
                        acid. In the subsequent fermentation, we also determined the best conditions
 +
                        of the engineered microorganism strains.<br><br> Therefore, we selected to control the carbon source, buffer, temperature, pH
 +
                        and other conditions to optimize the cell production process.</h4>
 +
                </div>
 +
            </div>
 +
        </div>
 +
        <!-- /.container -->
 +
        <img src="https://static.igem.org/mediawiki/2017/0/0c/Jz.png" class="img-responsive">
  
 +
    </div>
 +
    <!-- /.batu -->
  
  
  
  
 +
    <!-- Script to Activate the Carousel -->
 +
    <script>
 +
        $('.carousel').carousel({
 +
            interval: 5000 //changes the speed
 +
        })
 +
    </script>
 +
</body>
  
 
</html>
 
</html>

Latest revision as of 12:52, 1 November 2017

Abstract

Acrylic acid is a bulk chemical raw material, which is widely used in many fields because of its excellent polymerization capacity, such as paint, glue, and even mobile phone screen protective film. The average annual market demand of acrylic acid is up to 8 million tons, and the market value is nearly 10 billion US dollars, which shows a promising market prospect. At present, acrylic acid is made from propylene (which is obtained by petroleum cracking) after multi-step treatment, resulting in environmental pollution, high energy consumption and a lack of sustainablility.

This year, we aim to use a greener and more environmentally- friendly carbon source, glycerol, to achieve all green production of acrylic acid. Compared to traditional chemical synthesis methods, Synbio is relatively greener and more sustainable. Also, glycerol costs less than propene.

We constructed our cell factory based on 4 levels, which are—


Core Part

We use ceaS2 enzyme as the core part, but acrylic acid is a by-product of ceaS2 enzyme, whose catalytic effect of wild type is very weak with acrylic acid production only 1mg/L. Hence, we hope to improve the catalytic effect of ceaS2 enzyme.

We designed ceaS2 enzyme mutants via the AEMD(Auto Enzyme Mutation Design) platform and screened for ceaS2 mutants that own better acrylic acid yield by HPLC(High Performance Liquid Chromatography) and HTS(High throughput screening).

System

Respectively, E. coli and S. cerevisiae are the two sorts of model organisms that are most convenient to operate in the prokaryote and eukaryote. Therefore, in terms of our choice of the chassis organisms, we have E. coli MG1655 and S. cerevisiae BY4741 tested individually.

Pathway

We need to design two different metabolic pathways for two different chassis organisms and propose different optimization schemes. We introduced the ceaS2 enzyme exogenously on the basis of the glycerol metabolism of the two organisms, so that they could produce the target product acrylic acid using the intermediates G3P and DHAP.

Besides the construction of the pathways, we also reconstructed and optimized the original metabolic pathway to increase the carbon flux rate of the designed pathway and reduced the loss of by-pass carbon flux.

Production

All of the previous processes were applied in building the engineered microorganism strains which have a high production of acrylic acid. In the subsequent fermentation, we also determined the best conditions of the engineered microorganism strains.

Therefore, we selected to control the carbon source, buffer, temperature, pH and other conditions to optimize the cell production process.