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class="img-responsive"> | class="img-responsive"> | ||
− | <h4>Fig XX Purified | + | <h4>Fig XX Purified ceaS2 after Ni-NTA affinity chromatography |
<br> (M, protein marker (from top to bottom is 25、35、48、63、75、100、135、180 kDa);Lane1, precipitation samples | <br> (M, protein marker (from top to bottom is 25、35、48、63、75、100、135、180 kDa);Lane1, precipitation samples | ||
in the cell lysates; 2, supernatant samples in the cell lysates; 3, supernatant flow through Ni-NTA | in the cell lysates; 2, supernatant samples in the cell lysates; 3, supernatant flow through Ni-NTA | ||
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<h4> | <h4> | ||
− | Fig XX Identification function of | + | Fig XX Identification function of ceaS2 by liquid chromatogram |
<br> (The black line is acrylic acid standard, green line is control, blue line is reaction of D-G3P | <br> (The black line is acrylic acid standard, green line is control, blue line is reaction of D-G3P | ||
as substrate, red line is reaction DHAP as substrate) | as substrate, red line is reaction DHAP as substrate) | ||
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minutes presenting a single and satisfying peak shape. The samples in the control group did not correspond | minutes presenting a single and satisfying peak shape. The samples in the control group did not correspond | ||
with the treatment group. D-G3P and DHAP reaction group samples both had peaks, in line with the | with the treatment group. D-G3P and DHAP reaction group samples both had peaks, in line with the | ||
− | standard sample of acrylic acid. Therefore, we can initially determine that | + | standard sample of acrylic acid. Therefore, we can initially determine that ceas2 is able to catalyze |
D-G3P and DHAP to generate acrylic acid. | D-G3P and DHAP to generate acrylic acid. | ||
</h4> | </h4> | ||
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<h4> | <h4> | ||
− | Fig XX LC-MS of | + | Fig XX LC-MS of ceaS2 generated acrylic acid |
− | <br> (From top to bottom is acrylic acid standard, control, | + | <br> (From top to bottom is acrylic acid standard, control, ceas2 reaction by DHAP as substrate, secondary |
mass spectrum of acrylic acid) | mass spectrum of acrylic acid) | ||
<br> According to the liquid chromatogram XX, the control group had no peaks, and the retention time | <br> According to the liquid chromatogram XX, the control group had no peaks, and the retention time | ||
and molecular weight of the sample group were consistent with the standard sample of acrylic acid. | and molecular weight of the sample group were consistent with the standard sample of acrylic acid. | ||
Also, the molecular ion peaks and fragment ion peaks in the secondary mass spectrum of the sample | Also, the molecular ion peaks and fragment ion peaks in the secondary mass spectrum of the sample | ||
− | also fully demonstrated that | + | also fully demonstrated that ceas2 catalyzed DHAP or ceaS2 to produce the acrylic acid. |
<br> | <br> | ||
</h4> | </h4> |
Revision as of 15:16, 1 November 2017