Difference between revisions of "Team:Bielefeld-CeBiTec/Notebook/Methods"
Dbergen195 (Talk | contribs) |
Dbergen195 (Talk | contribs) |
||
| Line 41: | Line 41: | ||
<li><a href="https://static.igem.org/mediawiki/2017/a/a7/T--Bielefeld-CeBiTec--protocol_LB.pdf" target="blank">Lysogeny Broth (LB)</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/a/a7/T--Bielefeld-CeBiTec--protocol_LB.pdf" target="blank">Lysogeny Broth (LB)</a></li> | ||
<li><a href="https://static.igem.org/mediawiki/2017/8/8a/T--Bielefeld-CeBiTec--protocol_Monarch_Miniprep_kit_NEB.pdf" target="blank">Monarch® Plasmid Miniprep Kit (NEB)</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/8/8a/T--Bielefeld-CeBiTec--protocol_Monarch_Miniprep_kit_NEB.pdf" target="blank">Monarch® Plasmid Miniprep Kit (NEB)</a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2017/0/0e/T--Bielefeld-CeBiTec--protocol_Monarch_PCR_and_DNA_clean_up_kit_NEB.pdf" target="blank">Monarch® PCR & DNA Cleanup Kit (NEB)</a></li> | ||
| + | <li><a href="https://static.igem.org/mediawiki/2017/7/7d/T--Bielefeld-CeBiTec--protocol_MOPS.pdf" target="blank">MOPS Minimal Medium</a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
<div class="half right"> | <div class="half right"> | ||
<ul style="list-style-type: none"> | <ul style="list-style-type: none"> | ||
| − | |||
| − | |||
<li><a href="https://static.igem.org/mediawiki/2017/e/e6/T--Bielefeld-CeBiTec--protocol_native_PAGE.pdf" target="blank">Native DNA PAGE</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/e/e6/T--Bielefeld-CeBiTec--protocol_native_PAGE.pdf" target="blank">Native DNA PAGE</a></li> | ||
<li><a href="https://static.igem.org/mediawiki/2017/6/63/T--Bielefeld-CeBiTec--protocol_PBJR_buffer.pdf" target="blank">PBJR buffer</a></li> | <li><a href="https://static.igem.org/mediawiki/2017/6/63/T--Bielefeld-CeBiTec--protocol_PBJR_buffer.pdf" target="blank">PBJR buffer</a></li> | ||
Revision as of 19:58, 1 November 2017
Methods
Protocols
This page lists all regular methods used during our project. Special methods, which are not listed here, are described or linked on the corresponding result pages.
- 50xTAE Stock Solution
- Acetone Precipitation
- Biotinylation of glass slides
- Blunt end ligation
- Bradford estimation using RotiNanoquant
- Cloning Strategy with Restriction Enzymes
- Colony PCR GoTaq® G2 DNA Polymerase
- Fast cell lysis sample buffer
- Fast cell lysis for SDS-PAGE
- GeneJET Genomic DNA Purification Kit (Thermo Fisher Scientific)
- GeneJET Genomic DNA Purification Kit (Thermo Fisher Scientific)
- Generating chemocompetent cells
- Generating electrocompetent cells
- Genome editing via the CRISP-Cas9 system
- Gibson Assembly
- innuPREP Gel Extraktion Kit (Analytik Jena)
- Lycopene extraction
- Lysogeny Broth (LB)
- Monarch® Plasmid Miniprep Kit (NEB)
- Monarch® PCR & DNA Cleanup Kit (NEB)
- MOPS Minimal Medium
- Native DNA PAGE
- PBJR buffer
- PCR with Phusion High Fidelity Master Mix with HC enhancer
- PCR with Q5 High-Fidelity Polymerase
- Plasmid Assembly Protocol with Golden Gate Assembly
- Protein Purification Impact Protocol
- PureYieldTM Plasmid Miniprep System (Promega)
- SDS-PAGE
- SiO2 Column Chromatography with Gradient
- SDS-PAGE running buffer
- SOC-medium
- ssDNA Annealing
- Standard BioBrick Assembly
- Synthesis of CBT-asparagine
- TAE buffer
- TLC analysis
- Transformation via electroporation
- Transformation via heat shock
- "Typical" Restriction Digest
- Wizard® SV Gel and PCR Clean-Up System (Promega)
- ZymoPURETM Plasmid Miniprep Kit (Zymo Research)
Enzymes
Digestion enzymes
| Enzyme | Producer |
|---|---|
| AgeI-HF | NEB |
| BsaI | NEB |
| DpnI | NEB |
| DpnI | Jena Bioscience |
| EciI | NEB |
| EcoRV | Fermentas |
| MnlI | NEB |
| NotI | Jena Bioscience |
| PstI | NEB |
| PstI | Thermo Fisher Scientific |
| PstI-HF | NEB |
| SapI | NEB |
| SpeI | NEB |
| XbaI | NEB |
| XbaI | Jena Bioscience |
Polymerases
| Enzyme | Producer |
|---|---|
| Go Taq DNA polymerase | Promega |
| Klenow Fragment | Thermo Fisher Scientific |
| Phusion High-Fidelity DNA polymerase | NEB |
| Q5 High-Fidelity DNA polymerase | NEB |
| Taq Polymerase | Jena Bioscience |
Other Enzymes
| Enzyme | Producer |
|---|---|
| Shrimp Alkaline Phosphatase | Jena Bioscience |
| T4 DNA Ligase | NEB |
| T4 DNA Ligase | Thermo Fisher Scientific |
| T4 Polynucleotide Kinase | NEB |
