Difference between revisions of "Team:Uppsala/Description"

 
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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<li> A clear and concise description of your project.</li>
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<h5>Advice on writing your Project Description</h5>
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
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      <div style="padding-bottom: 2%;"> This year iGEM Uppsala is Crafting Crocin. Crocin is an apocarotenoid (organic pigment) found in saffron, extracted from <i>Crocus sativus</i>. Crocin, crocetin, zeaxanthin and other organic compounds in the crocin pathway, are responsible for saffron's  beautiful crimson color, delicious taste and characteristic aroma. Recent studies suggest that crocin has several medicinal properties like helping with inflammation (1), neurodegenerative diseases (2) and many more. We have worked on the pathway from zeaxanthin to crocin – extended from the pathway of farnesyl pyrophosphate (FPP) to zeaxanthin.</div>
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        <figcaption class="figure-caption figtext" style="padding-bottom: 2%; text-align:center;"> Figure 1. The pathway with enzymes of farnesyl pyrophospate to crocin. </figcaption>
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      <div style="padding-bottom: 2%;">Due to their color, the pathway compounds have great potential as organic dyes for industrial applications. However, the labour-intensive production of saffron makes the product very expensive and the crocin pathway is poorly characterized. By using synthetic biology for recombinant expression in <i>E. coli</i> we hope to reduce the price of the compounds within the pathway and open up the possibility for industrial and medicinal applications to be further explored.
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          <div style="padding-bottom: 2%;"> Previous work has been done with the five genes from FPP to zeaxanthin, but this operon is very large and unstable. We used chromosomal integration of these genes to ensure stable expression of zeaxanthin and allow for a decrease in antibiotics usage. The iGEM Uppsala 2013 Team tried to express the compounds after zeaxanthin in the biosynthetic pathway, but failed. To extend the pathway with the three-steps leading from zeaxanthin to crocin we successfully identified, assembled and characterized three enzymes in order to explore the possibility of using bacterial production of the color intense compounds.</div>
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      <div style="padding-bottom: 2%;"> To learn more about our zeaxanthin producing strain, <a href="https://2017.igem.org/Team:Uppsala/Zea-Strain">click here</a>!</div>
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      <div style="padding-bottom: 2%;"> To learn more about our crocin pathway, <a href="https://2017.igem.org/Team:Uppsala/CrocinPathway">click here</a>!</div>
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          <div style="padding-bottom: 1%;"> <b>References </b></div>
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          <div> (1) Papandreou MA, Kanakis CD, Polissiou MG, Efthimiopoulos S, Cordopatis P, Margarity M, et al. Inhibitory Activity on Amyloid-β Aggregation and Antioxidant Properties of Crocus sativus Stigmas Extract and Its Crocin Constituents. J Agric Food Chem. 2006 Nov 1;54(23):8762–8.
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          <div style="padding-bottom: 2%;"> (2) Chen L, Qi Y, Yang X. Neuroprotective effects of crocin against oxidative stress induced by ischemia/reperfusion injury in rat retina. Ophthalmic Res. 2015;54(3):157–68.
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<p>See how other teams have described and presented their projects: </p>
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<li><a href="https://2016.igem.org/Team:Imperial_College/Description">2016 Imperial College</a></li>
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<li><a href="https://2016.igem.org/Team:Wageningen_UR/Description">2016 Wageningen UR</a></li>
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> 2014 UC Davis</a></li>
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">2014 SYSU Software</a></li>
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Latest revision as of 23:24, 1 November 2017

<!DOCTYPE html> Description Page

What

This year iGEM Uppsala is Crafting Crocin. Crocin is an apocarotenoid (organic pigment) found in saffron, extracted from Crocus sativus. Crocin, crocetin, zeaxanthin and other organic compounds in the crocin pathway, are responsible for saffron's beautiful crimson color, delicious taste and characteristic aroma. Recent studies suggest that crocin has several medicinal properties like helping with inflammation (1), neurodegenerative diseases (2) and many more. We have worked on the pathway from zeaxanthin to crocin – extended from the pathway of farnesyl pyrophosphate (FPP) to zeaxanthin.
Figure 1. The pathway with enzymes of farnesyl pyrophospate to crocin.

Why

Due to their color, the pathway compounds have great potential as organic dyes for industrial applications. However, the labour-intensive production of saffron makes the product very expensive and the crocin pathway is poorly characterized. By using synthetic biology for recombinant expression in E. coli we hope to reduce the price of the compounds within the pathway and open up the possibility for industrial and medicinal applications to be further explored.

How

Previous work has been done with the five genes from FPP to zeaxanthin, but this operon is very large and unstable. We used chromosomal integration of these genes to ensure stable expression of zeaxanthin and allow for a decrease in antibiotics usage. The iGEM Uppsala 2013 Team tried to express the compounds after zeaxanthin in the biosynthetic pathway, but failed. To extend the pathway with the three-steps leading from zeaxanthin to crocin we successfully identified, assembled and characterized three enzymes in order to explore the possibility of using bacterial production of the color intense compounds.
To learn more about our zeaxanthin producing strain, click here!
To learn more about our crocin pathway, click here!
References
(1) Papandreou MA, Kanakis CD, Polissiou MG, Efthimiopoulos S, Cordopatis P, Margarity M, et al. Inhibitory Activity on Amyloid-β Aggregation and Antioxidant Properties of Crocus sativus Stigmas Extract and Its Crocin Constituents. J Agric Food Chem. 2006 Nov 1;54(23):8762–8.
(2) Chen L, Qi Y, Yang X. Neuroprotective effects of crocin against oxidative stress induced by ischemia/reperfusion injury in rat retina. Ophthalmic Res. 2015;54(3):157–68.