Difference between revisions of "Team:Bulgaria/improve"

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         <h1 style="color:#1AA55E; font-family: Comic sans MS;"> Improve </h1>
 
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           <b>Bronze medal contribution</b>
 
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             For teams seeking to improve upon a previous part or project, you should document all of your work on this page. Please remember to include all part measurement and characterization data on the part page on the Regisrty. Please include a link to your improved part on this page.
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             We improved Part Part:BBa_K145201 (INPUT TetR generator). Initially we designed an expression construct, composed by parts BBa_K145201 and BBa_K081012(GFP Generator). When we expressed this construct in the high copy number vector pSB1C3, we found that a significant basal level of GFP expression can be detected without adding an inducer. This is present on the picture given below (left tube - no induction, right tube - induction with tetracycline).<br /><br />
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  <a href="https://imgur.com/3shbtjA"><img src="https://i.imgur.com/3shbtjA.jpg" title="source: imgur.com" /></a>
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Revision as of 23:47, 1 November 2017

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Improve


Bronze medal contribution


We improved Part Part:BBa_K145201 (INPUT TetR generator). Initially we designed an expression construct, composed by parts BBa_K145201 and BBa_K081012(GFP Generator). When we expressed this construct in the high copy number vector pSB1C3, we found that a significant basal level of GFP expression can be detected without adding an inducer. This is present on the picture given below (left tube - no induction, right tube - induction with tetracycline).



Gold Medal Criterion #2

Standard Tracks: Improve the function of an existing BioBrick Part. The original part must NOT be from your 2017 part number range. If you change the original part sequence, you must submit a new part. In addition, both the new and original part pages must reference each other. This working part must be different from the part documented in bronze #4 and silver #1.

Special Tracks: Improve the function of an existing iGEM project (that your current team did not originally create) and display your achievement on your wiki.