Difference between revisions of "Team:Freiburg"

 
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           <div class="cool_content cool_text_blink link_trigger" href='' title="">
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           <div class="cool_content cool_text_blink link_trigger" href="https://2017.igem.org/Team:Freiburg/Achievements" title="">
 
           <div class="text">
 
           <div class="text">
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             Besides learning a lot and having tremendous fun, we achieved something great this year!
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             Besides learning <br> a lot and having fun, we achieved something great this year!
 
Check it out!
 
Check it out!
  
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           </div>
 
           </div>
 
           </div>
 
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<div class="cool_header"><a href=''>Achievement</a></div>
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<div class="cool_header"><a href="https://2017.igem.org/Team:Freiburg/Achievements">Achievements</a></div>
 
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           <div class="cool_content cool_text_blink link_trigger" href="https://2017.igem.org/Team:Freiburg/Team" title="">
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            Find out more about the Freiburg 2017 Team!
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            <br>Find out more <br> about the Freiburg 2017 team and our members!
 
           </div>
 
           </div>
 
           </div>
 
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<div class="cool_header"><a href=''>Team</a></div>
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<div class="cool_header"><a href='https://2017.igem.org/Team:Freiburg/Team'>Team</a></div>
 
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           <div class="cool_content cool_text_blink link_trigger" href="https://2017.igem.org/Team:Freiburg/Design" title="AND gate">
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            In our project, we used a genetic AND gate to improve the safety of CAR therapy! Learn more about it here...
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            To make our approach safer, we are integrating two inputs into one output. Have a look!
  
 
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<div class="cool_header"><a href=''>AND gate</a></div>
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<div class="cool_header"><a href="https://2017.igem.org/Team:Freiburg/Design">AND Gate</a></div>
 
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               <div class="cool_content cool_text_blink link_trigger" href='https://2017.igem.org/Team:Freiburg/Results' title="">
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                   We generated stable cell lines, a knockout in two cell lines, characterized x promoters and much more! Check out our results page!
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                   We generated <br> stable cell lines, a gene knockdown, characterized several promoters and much more!
 
               </div>
 
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<div class="cool_header"><a href=''>Results</a></div>
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<div class="cool_header"><a href="https://2017.igem.org/Team:Freiburg/Results">Results</a></div>
 
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             <div class="cool_content cool_text_blink link_trigger" href='' title="">
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             <div class="cool_content cool_text_blink link_trigger" href='https://2017.igem.org/Team:Freiburg/Model' title="">
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               To better understand our project, we modeled gene expression within our genetic circuit and T cell migration. Learn more here...
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               To better <br> understand our AND gate, we modeled gene expression within our genetic circuit.
 
             </div>
 
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<div class="cool_header"><a href=''>Modeling</a></div>
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<div class="cool_header"><a href='https://2017.igem.org/Team:Freiburg/Model'>Modeling</a></div>
 
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             <div class="cool_content cool_text_blink link_trigger" href='https://2017.igem.org/Team:Freiburg/HP/Silver' title="">
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                 In human practice, we wanted to hear the thoughts and concerns about our project. Check out our survey and interviews with experts working on the field!
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                 To hear thoughts about our project, check out our survey and interviews with experts working on the topic!
 
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<div class="cool_header"><a href=''>Human Practice</a></div>
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<div class="cool_header"><a href='https://2017.igem.org/Team:Freiburg/HP/Silver'>Human Practice</a></div>
 
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        <a href="https://2017.igem.org/Team:Freiburg/Introduction" title="Start guided tour"> <img  src="https://static.igem.org/mediawiki/2017/7/74/T-FREIBURG-cartel.png" height="150px" width="340px"></a>
 
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<h1 align="center" style="font-size: 120%">CARTEL<sup style="font-size: 60%">TM</sup><br><br>Chimeric Antigen Receptor on T Cells Expressed Locally in the Tumor Microenvironment</h1>
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<h2 style="font-size:40px; padding-top: 50px" align="center">Abstract</h2>
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<p>A new approach to cancer treatment is the chimeric antigen receptor (CAR) T cell therapy, which shows promising results fighting tumors in clinical trials. It consists of autologous isolated T cells modified with a chimeric receptor based on the T-cell receptor combined with the recognition domain of an antibody. Upon reinjection, CAR T cells exhibit cytotoxicity with high affinity towards cells displaying the antigen. However, clinical trials have shown that as tumor antigens are not solely expressed on tumor cells, but also on healthy tissues, grave off-target effects like the Graft-versus-Host-Disease may occur. In order to avoid such side-effects, we engineer CAR T cell lines specifically activated by factors of the tumor microenvironment. Controlled by a genetic AND gate system the T cells need two input signals in order to express CAR. This would allow highly localized cytotoxic activity of T cells and provide safer cell-based cancer immunotherapy especially for solid tumors.</p>
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<p>A new approach to cancer treatment is the chimeric antigen receptor (CAR) therapy, which shows promising results fighting tumors in clinical trials. It consists of autologous isolated T cells modified with a chimeric receptor based on the T-cell receptor combined with the recognition domain of an antibody. Upon reinjection, CAR T cells exhibit cytotoxicity with high affinity towards cells displaying the antigen. However, clinical trials have shown that as tumor antigens are not solely expressed on tumor cells, but also on healthy tissues, grave off-target effects like the Graft-versus-Host-Disease may occur. In order to avoid such side-effects, we engineer CAR T cell lines specifically activated by factors of the tumor microenvironment. Controlled by a genetic AND gate system the T cells need two input signals in order to express CAR. This would allow highly localized cytotoxic activity of T cells and provide safer cell-based cancer immunotherapy especially for solid tumors.</p>
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{{Freiburg/Footer}}
 
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Latest revision as of 01:52, 2 November 2017

Logo
Overview Motivation CAR T Cells Tumor Microenvironment AND Gate Outlook Achievements References
Members Attributions Partners Contact
Main Project Modeling Applied Design Proof of Concept BioBricks Basic Part BioBrick Improvement Interlab Study
Safety Cloning HIF1A Knockdown Cell Culture Lab Book
Human Practice Integrated Human Practice Collaborations
  • Besides learning
    a lot and having fun, we achieved something great this year! Check it out!
    Achievements

  • Find out more
    about the Freiburg 2017 team and our members!
    Team
  • To make our approach safer, we are integrating two inputs into one output. Have a look!
    AND Gate
  • We generated
    stable cell lines, a gene knockdown, characterized several promoters and much more!
    Results
  • To better
    understand our AND gate, we modeled gene expression within our genetic circuit.
    Modeling
  • To hear thoughts about our project, check out our survey and interviews with experts working on the topic!
    Human Practice

Abstract

A new approach to cancer treatment is the chimeric antigen receptor (CAR) T cell therapy, which shows promising results fighting tumors in clinical trials. It consists of autologous isolated T cells modified with a chimeric receptor based on the T-cell receptor combined with the recognition domain of an antibody. Upon reinjection, CAR T cells exhibit cytotoxicity with high affinity towards cells displaying the antigen. However, clinical trials have shown that as tumor antigens are not solely expressed on tumor cells, but also on healthy tissues, grave off-target effects like the Graft-versus-Host-Disease may occur. In order to avoid such side-effects, we engineer CAR T cell lines specifically activated by factors of the tumor microenvironment. Controlled by a genetic AND gate system the T cells need two input signals in order to express CAR. This would allow highly localized cytotoxic activity of T cells and provide safer cell-based cancer immunotherapy especially for solid tumors.