Difference between revisions of "Team:Arizona State/Parts"

 
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<p> Both receivers described below were designed to be inserted into a modular receiver vector pSB1C3 with an inducible promoter, a constitutive promoter, a green fluorescent protein (GFP) 2 ribosomal binding sites (RBS)- one for the respective regulator protein and one for a Green Fluorescent Protein </p>
  
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<h2>2017 New Receiver Systems </h2>
  
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<table style="width:100%" align="center">
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<tr>
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    <th>Part Name</th>
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    <th>Part Number</th>
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    <th>Part Type</th>
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  </tr>
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  <tr>
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    <td> TraR </td>
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    <td> <a href="http://parts.igem.org/Part:BBa_K2357028">BBa_B2357028</a> </td>
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    <td>Reciever</td>
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  </tr>
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    <td> LasR </td>
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    <td> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2357000">BBa_K2357000</a> </td>
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    <td>Receiver</td>
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</table>
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<h2>Receiver Design Flowchart</h2>
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<center><img src="https://static.igem.org/mediawiki/2017/8/87/R_flowchart.PNG" alt="Design Flowchart" style="max-width: 800px; width: 100%"></center>
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<h2>Receiver Design Flowchart for Cloning</h2>
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<center><img src="https://static.igem.org/mediawiki/2017/e/e4/Gblock_flowchart.PNG" alt="Design Flowchart 2" style="max-width: 900px; width: 100%"></center>
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<center><img src="https://static.igem.org/mediawiki/2017/4/4a/Cloning_process_design.PNG" alt="Design Flowchart 3" style="max-width:900px; width: 100%"></center>
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<h2>Receiver Design</h2>
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<center><img src="https://static.igem.org/mediawiki/2017/9/92/Receiver_design_with_sbol.PNG" alt="Design Flowchart 2" style="max-width: 900px; width: 100%"></center>
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<p> In our receiver design, there a multitude of parts attained that are already Bio-Brick verified. The table below shows the various parts from the BioBrick database used in our design. Promoters, ribosomal binding sites, terminators and green fluorescent protein were chosen. In addition to this information, the reason and description of how each works in our system is described.
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<tr>
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    <th>Receiver Part Names</th>
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    <th>Part Number</th>
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    <th>Description</th>
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  </tr>
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  <tr>
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    <td> Ribosomal binding site for GFP </td>
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    <td><a href="http://parts.igem.org/Part:BBa_B0032">BBa_B0032</a> </td>
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    <td> Stronger relative strength compared to other Ribosomal Binding Sites</td>
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  </tr>
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  <tr>
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    <td> Green Fluorescent Protein </td>
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    <td> <a href="http://parts.igem.org/Part:BBa_E0040">BBa_E0040</a>  </td>
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    <td> Green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP </td>
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  </tr>
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  <tr>
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    <td> Terminator for Green Fluorescent Protein </td>
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    <td> <a href="http://parts.igem.org/Part:BBa_J61048:Design">BBa_J61048</a> </td>
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    <td> Terminates the expression of GFP</td>
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  </tr>
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  <tr>
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    <td> Promoter for Receiver Protein </td>
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    <td> <a href="http://parts.igem.org/Part:BBa_J23100">BBa_J23100</a> </td>
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    <td> Stronger promoter relative to the GFP promoter for stronger transcription </td>
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</td>
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  </tr>
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  <tr>
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    <td> Ribosomal Binding Site for Receiver Protein </td>
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    <td> <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a> </td>
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    <td>Ribosomal binding site for receiver protein </td>
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  </tr>
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  <tr>
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    <td> Terminator for Receiver Protein </td>
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    <td> <a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a> </td>
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    <td> Strong terminator to end transcription of receiver promoter; A combination of RBS BBa_B0010 and BBa_B0012</td>
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  </tr>
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</table>
  
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<h1>Parts</h1>
 
  
  
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<h5>What information do I need to start putting my parts on the Registry?</h5>
 
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<p>The information needed to initially create a part on the Registry is:</p>
 
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<li>Part Name</li>
 
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<li>Part type</li>
 
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<li>Creator</li>
 
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<li>Sequence</li>
 
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<li>Short Description (60 characters on what the DNA does)</li>
 
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<li>Long Description (Longer description of what the DNA does)</li>
 
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<li>Design considerations</li>
 
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</ul>
 
  
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
 
  
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<h5>Part Table </h5>
 
  
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
 
  
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<groupparts>iGEM17 Arizona_State</groupparts>
 
<groupparts>iGEM17 Arizona_State</groupparts>
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Latest revision as of 02:54, 2 November 2017

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