Both receivers described below were designed to be inserted into a modular receiver vector pSB1C3 with an inducible promoter, a constitutive promoter, a green fluorescent protein (GFP) 2 ribosomal binding sites (RBS)- one for the respective regulator protein and one for a Green Fluorescent Protein
| Part Name | Part Number | Part Type |
|---|---|---|
| TraR | BBa_B2357028 | Reciever |
| LasR | BBa_K2357000 | Receiver |
In our receiver design, there a multitude of parts attained that are already Bio-Brick verified. The table below shows the various parts from the BioBrick database used in our design. Promoters, ribosomal binding sites, terminators and green fluorescent protein were chosen. In addition to this information, the reason and description of how each works in our system is described.
| Receiver Part Names | Part Number | Description |
|---|---|---|
| Ribosomal binding site for GFP | BBa_B0032 | Stronger relative strength compared to other Ribosomal Binding Sites |
| Green Fluorescent Protein | BBa_E0040 | Green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP |
| Terminator for Green Fluorescent Protein | BBa_J61048 | Terminates the expression of GFP |
| Promoter for Receiver Protein | BBa_J23100 | Stronger promoter relative to the GFP promoter for stronger transcription |
| Ribosomal Binding Site for Receiver Protein | BBa_B0034 | Ribosomal binding site for receiver protein |
| Terminator for Receiver Protein | BBa_B0015 | Strong terminator to end transcription of receiver promoter; A combination of RBS BBa_B0010 and BBa_B0012 |
