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− | <!-----------Thank you to the 2016 WashU iGEM team for the | + | <!-----------Thank you to the 2016 WashU iGEM team for the some of the code and the set-up for our lab notebook!------------------> |
<style> | <style> | ||
+ | |||
+ | * { | ||
+ | font-family:"Trebuchet MS"; | ||
+ | } | ||
/* Slideshow container */ | /* Slideshow container */ | ||
− | .slideshow-container { | + | .slideshow-container {au |
//max-width: 1000px; | //max-width: 1000px; | ||
position: relative; | position: relative; | ||
− | + | margin-left:10vw; | |
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/* Next & previous buttons */ | /* Next & previous buttons */ | ||
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.month-calendar { | .month-calendar { | ||
− | width: | + | width: 80vw; |
//float:left; | //float:left; | ||
padding: 5px 5px 5px 5px; | padding: 5px 5px 5px 5px; | ||
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margin-right: auto; | margin-right: auto; | ||
display:block; | display:block; | ||
− | background: | + | background:white; |
padding: 30px 50px 30px 50px; | padding: 30px 50px 30px 50px; | ||
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− | < | + | <p style="text-align:center; font-size:4vw">Notebook</p> |
− | <p style="text-align: center"><em>Click on a date to see what we did on that day! | + | <p style="text-align: center"><em>Click on a date to see what we did on that day!</em></p> |
− | <div class = "calendar-wrapper"> | + | <div class = "calendar-wrapper" style="font-size:1.5vw"> |
− | <div class="month-calendar june" style = "display:block;"> | + | <div class="month-calendar june" style = "display:block;"> |
<table> | <table> | ||
<tr> | <tr> | ||
− | <th colspan=7>June 2017</th> | + | <th colspan=7 style="background-color:#D5FDD5; border: 0.2vw solid #8CFA8C; color:#3c643c">June 2017</th> |
+ | </tr> | ||
+ | <tr> | ||
+ | <th>Sunday</th> | ||
+ | <th>Monday</th> | ||
+ | <th>Tuesday</th> | ||
+ | <th>Wednesday</th> | ||
+ | <th>Thursday</th> | ||
+ | <th>Friday</th> | ||
+ | <th>Saturday</th> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
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<table> | <table> | ||
<tr> | <tr> | ||
− | <th colspan=7>July 2017</th> | + | <th colspan=7 style="border:0.2vw solid #93efea;background-color:#d6f9f7; color:#597A8A">July 2017</th> |
+ | </tr> | ||
+ | <tr> | ||
+ | <th>Sunday</th> | ||
+ | <th>Monday</th> | ||
+ | <th>Tuesday</th> | ||
+ | <th>Wednesday</th> | ||
+ | <th>Thursday</th> | ||
+ | <th>Friday</th> | ||
+ | <th>Saturday</th> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
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<table> | <table> | ||
<tr> | <tr> | ||
− | <th colspan=7>August 2017</th> | + | <th colspan=7 style="background-color:#D5FDD5; border: 0.2vw solid #8CFA8C; color:#3c643c">August 2017</th> |
+ | </tr> | ||
+ | <tr> | ||
+ | <th>Sunday</th> | ||
+ | <th>Monday</th> | ||
+ | <th>Tuesday</th> | ||
+ | <th>Wednesday</th> | ||
+ | <th>Thursday</th> | ||
+ | <th>Friday</th> | ||
+ | <th>Saturday</th> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
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<table> | <table> | ||
<tr> | <tr> | ||
− | <th colspan=7>September 2017</th> | + | <th colspan=7 style="border:0.2vw solid #93efea;background-color:#d6f9f7; color:#597A8A">September 2017</th> |
+ | </tr> | ||
+ | <tr> | ||
+ | <th>Sunday</th> | ||
+ | <th>Monday</th> | ||
+ | <th>Tuesday</th> | ||
+ | <th>Wednesday</th> | ||
+ | <th>Thursday</th> | ||
+ | <th>Friday</th> | ||
+ | <th>Saturday</th> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
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<table> | <table> | ||
<tr> | <tr> | ||
− | <th colspan=7>October 2017</th> | + | <th colspan=7 style="background-color:#D5FDD5; border: 0.2vw solid #8CFA8C; color:#3c643c">October 2017</th> |
+ | </tr> | ||
+ | <tr> | ||
+ | <th>Sunday</th> | ||
+ | <th>Monday</th> | ||
+ | <th>Tuesday</th> | ||
+ | <th>Wednesday</th> | ||
+ | <th>Thursday</th> | ||
+ | <th>Friday</th> | ||
+ | <th>Saturday</th> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
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</div> | </div> | ||
− | <!-- ************************* | + | <!-- ************************* Notebook Content ************************* --> |
<div class="slideshow-container"> | <div class="slideshow-container"> | ||
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<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Thursday, June 1</u></strong></p> | ||
<p><em>Present: Micah, Maddie, Alex</em></p> | <p><em>Present: Micah, Maddie, Alex</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
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<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Friday, June 2</u></strong></p> | ||
<p><em>Present: Micah, Maddie</em></p> | <p><em>Present: Micah, Maddie</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
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<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
− | <p>No | + | <p><strong><u>Saturday, June 3</u></strong></p> |
+ | <p>No work done today!</p> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
− | <p>No | + | <p><strong><u>Sunday, June 4</u></strong></p> |
+ | <p>No work done today!</p> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Monday, June 5</u></strong></p> | ||
<p><em>Present: Micah, Maddie, Alex, Mark, Collin</em></p> | <p><em>Present: Micah, Maddie, Alex, Mark, Collin</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
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<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Tuesday, June 6</u></strong></p> | ||
<p><em>Present: Micah, Maddie, Alex, Mark, Collin</em></p> | <p><em>Present: Micah, Maddie, Alex, Mark, Collin</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
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<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Wednesday, June 7</u></strong></p> | ||
<p><em>Present: Micah, Maddie, Mark, Collin, Alex</em></p> | <p><em>Present: Micah, Maddie, Mark, Collin, Alex</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
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<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Thursday, June 8</u></strong></p> | ||
<p><em> Present: Alex, Maddie, Micah, Collin, Mark, Zoe </em></p> | <p><em> Present: Alex, Maddie, Micah, Collin, Mark, Zoe </em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
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<ul> | <ul> | ||
<li> | <li> | ||
− | <img src="https://static.igem.org/mediawiki/2017/3/33/T--WashU_StLouis--2017-06-08_BBa_B0034_in_MHD42_Transformation.jpg" style="height: | + | <img src="https://static.igem.org/mediawiki/2017/3/33/T--WashU_StLouis--2017-06-08_BBa_B0034_in_MHD42_Transformation.jpg" style="height:11vw;width:11vw"> |
− | <img src="https://static.igem.org/mediawiki/2017/6/65/T--WashU_StLouis--2017-06-08_BBa_R0010_in_MHD42_Transformation.jpg" style="height: | + | <img src="https://static.igem.org/mediawiki/2017/6/65/T--WashU_StLouis--2017-06-08_BBa_R0010_in_MHD42_Transformation.jpg" style="height:11vw;width:11vw"> |
− | <img src="https://static.igem.org/mediawiki/2017/1/14/T--WashU_StLouis--2017-06-08_BBa_K1499200_in_MHD42_Transformation.jpg" style="height: | + | <img src="https://static.igem.org/mediawiki/2017/1/14/T--WashU_StLouis--2017-06-08_BBa_K1499200_in_MHD42_Transformation.jpg" style="height:11vw;width:11vw"> |
</li> | </li> | ||
</ul> | </ul> | ||
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<li> Attended Energy, Environmental, and Chemical Engineering (EECE) summer interns orientation <em> [Collin, Mark, | <li> Attended Energy, Environmental, and Chemical Engineering (EECE) summer interns orientation <em> [Collin, Mark, | ||
Maddie, Zoe, Alex, Micah] </em> </li> | Maddie, Zoe, Alex, Micah] </em> </li> | ||
− | <li> Discussed each member's designated role and plans for the future </li> | + | <li> Discussed each member's designated role and plans for the future <em> [Collin, Mark, Maddie, Zoe, Alex, Micah] </em> </li> |
− | <li> Made a team calendar </li> | + | <li> Made a team calendar <em> [Collin, Mark, Maddie, Zoe, Alex, Micah] </em> </li> |
</ol> | </ol> | ||
</div> | </div> | ||
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<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
− | <p><em></em></p> | + | <p><strong><u>Friday, June 9</u></strong></p> |
+ | <p><em> Present: Maddie, Zoe, Micah, Mark, Collin, Alex</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li> Checked results for competency of DH5α cells <em> [Alex, Mark, Micah, Maddie, Zoe, Collin} </em> </li> | |
+ | <ul> | ||
+ | <li> Test was successful! The cells had a moderate transformation efficiency. </li> | ||
+ | <li> <img src = "https://static.igem.org/mediawiki/2017/8/88/T--WashU_StLouis--2017-06-09_DH5alpha_Comp_Cells_Test_Results.jpg"/> </li> | ||
+ | </ul> | ||
+ | <li> Began the Interlab study by transforming the parts into DH5α cells <em>[Maddie, Zoe, Mark]</em> </li> | ||
+ | <ul> | ||
+ | <li> Parts: Postive control (BBa_I20270), Negative control (BBa_R0040), Test device 1 (BBa_J364000), Test device 2 (BBa_J364001), Test device 3 (BBa_J364002), Test device 4 (BBa_J364003), Test device 5 (BBa_J364004), Test device 6 (BBa_J364005)</li> | ||
+ | </ul> | ||
+ | <li> Made 400 mL of LB Agar <em> [Zoe] </em> </li> | ||
+ | <li> Made 11 Chloramphenicol (CM) + LB plates <em> [Collin, Mark, Alex] </em> </li> | ||
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li> Researched modelling ideas <em> [Micah] </em> </li> | ||
+ | </ol> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
− | <p><em></em></p> | + | <p><strong><u>Saturday, June 10</u></strong></p> |
+ | <p><em>Present: Zoe</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li> Checked on the transformations for the interlab study <em>[Zoe]</em></li> | |
+ | <ul> | ||
+ | <li>Colonies existed on all plates. Only a few colonies appeared on the plate with Test Device 1 (BBa_J364000)</li> | ||
+ | <li><img src="https://static.igem.org/mediawiki/2017/f/f5/T--WashU_StLouis--2017-06-10_Transformations_for_Interlab.jpg"></li> | ||
+ | <li> <strong>Top (from left to right):</strong> Postive control (BBa_I20270), Negative control (BBa_R0040), Test device 1 (BBa_J364000), Test device 2 (BBa_J364001) <strong>Bottom (from left to right):</strong> Test device 3 (BBa_J364002), Test device 4 (BBa_J364003), Test device 5 (BBa_J364004), Test device 6 (BBa_J364005)</li> | ||
+ | </ul> | ||
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
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<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
− | <p><em></em></p> | + | <p><strong><u>Sunday, June 11</u></strong></p> |
+ | <p><em>Present: Alex</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li>Prepared inoculated overnight cultures of the DH5α cells with BBa_B0034, BBa_R0010, and BBa_K1499200 in chloramphenicol <em>[Alex]</em> </li> | |
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
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<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
− | <p><em></em></p> | + | <p><strong><u>Monday, June 12</u></strong></p> |
+ | <p><em>Present: Micah, Zoe, Mark, Collin, Maddie, Alex</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li>Transformed part BBa_E004 (GFP) into MHD42 competent cells <em>[Zoe, Micah]</em></li> | |
+ | <li>Miniprepped the 3 separate cultures of MHD42 cells that contained BBa_R0010, BBa_B0034, and BBa_K1499200 <em>[Alex, Collin]</em></li> | ||
+ | <li>Measured concentration of DNA using the NanoDrop <em>[Alex, Collin]</em></li> | ||
+ | <li>Performed the Calibration Protocol for the Interlab study by measuring the absorbances of distilled water and Ludox HS-40 <em>[Maddie, Mark]</em></li> | ||
+ | <li>Measured the fluorescence of fluorescein serial dilutions for the Interlab study <em>[Mark, Maddie]</em></li> | ||
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li>Filled out Forms 1 and 2 for the Interlab study <em>[Maddie, Mark]</em></li> | ||
+ | <li>Studied BioBrick assembly methods <em> [Alex, Micah] </em></li> | ||
+ | <li>Met with Dr. Brennan and discussed our project thus far with her <em>[Micah, Collin, Alex, Maddie, Mark, Zoe]</em></li> | ||
+ | </ol> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
− | <p><em></em></p> | + | <p><strong><u>Tuesday, June 13</u></strong></p> |
+ | <p><em>Present: Zoe, Mark, Collin, Alex, Maddie, Micah</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li>Checked for the transformation of part BBa_E0040 (GFP) into MHD42 competent cells <em>[Micah]</em></li> | |
+ | <ul> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/f/f7/T--WashU_StLouis--2017-06-13_Transformations_of_BBa_E0040_%28GFP%29.jpg" style="height:11vw;width:11vw"/> | ||
+ | </ul> | ||
+ | <li>Miniprepped the DNA for parts BBa_R0010 and BBa_B0034 from the MHD42 cells that were cultured overnight <em>[Zoe, Micah]</em></li> | ||
+ | <li>Measured concentration of DNA using the NanoDrop <em>[Micah, Zoe]</em></li> | ||
+ | <li>Performed cell measurements for the Interlab study (Day 3) <em>[Maddie, Mark]</em></li> | ||
+ | <ul> | ||
+ | <li>Measured the OD of the each of the 16 cultures (2 colonies per control and test device) and diluted each so that the OD of each would be approximately 0.02 </li> | ||
+ | <li>Measured the OD of samples of each diluted culture after 0, 2, 4, and 6 hours </li> | ||
+ | </ul> | ||
+ | <li>Prepared overnight culture of the MHD42 cells with BBa_E0040 (GFP) <em>[Micah, Zoe]</em></li> | ||
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li>Discussed how perform restriction digest and ligation using the BioBrick kit with Dr. Brennan <em>[Alex, Collin]</em></li> | ||
+ | </ol> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
− | <p><em></em></p> | + | <p><strong><u>Wednesday, June 14</u></strong></p> |
+ | <p><em>Present: Zoe, Collin, Micah, Alex, Mark, Maddie</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li>Miniprepped 4 tubes of part BBa_E0040 (GFP) <em>[Zoe, Micah]</em></li> | |
+ | <li>Measured concentration of DNA using the NanoDrop <em>[Micah, Mark, Zoe]</em></li> | ||
+ | <li>Performed restriction digest of parts BBa_B0034 and BBa_R0010 <em>[Alex, Collin]</em></li> | ||
+ | <li>Performed gel electrophoresis of digested BBa_B0034 and BBa_R0010 <em>[Alex, Collin]</em></li> | ||
+ | <li>Prepared LB + Ampicillin plates <em>[Zoe, Micah, Mark]</em></li> | ||
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
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<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
− | <p><em></em></p> | + | <p><strong><u>Thursday, June 15</u></strong></p> |
+ | <p><em>Present: Mark, Zoe, Alex, Maddie, Collin, Micah</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li>Purified the digested BBa_R0010 and BBa_B0034 from the agarose gel <em>[Collin, Alex, Zoe]</em></li> | |
+ | <li>Ligated the lac promoter from BBa_R0010 to the RBS and pSB1A2 backbone of BBa_B0034 <em>[Alex]</em></li> | ||
+ | <li>Performed restriction digest of parts BBa_B0034 and BBa_R0010 <em>[Zoe, Collin]</em></li> | ||
+ | <li>Prepared LB Agar <em>[Zoe]</em></li> | ||
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li>Researched modelling ideas <em>[Micah, Maddie]</em></li> | ||
+ | <li>Researched possible parts in the registry to improve <em>[Mark]</em></li> | ||
+ | <li>Met with Dr. Brennan to discuss progress done so far <em>[Mark, Zoe, Maddie, Alex, Collin, Micah]</em></li> | ||
+ | </ol> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
− | <p><em></em></p> | + | <p><strong><u>Friday, June 16</u></strong></p> |
+ | <p><em>Present: Zoe, Maddie, Micah, Alex, Mark</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li> Checked on the transformation of the MHD42 E. coli cells with ligated plasmid consisting of RBS (from Part BBa_B0034) and the lac promoter (from Part BBa_R0010) <em>[Micah]</em></li> | |
+ | <ul> | ||
+ | <li><img src="https://static.igem.org/mediawiki/2017/1/12/T--WashU_StLouis--2017-06-16_Transformation_of_ligated_BBa_B0034_and_BBa_R0010.jpg" style="height:11vw;width:11vw"/></li> | ||
+ | <li> Prepared lab materials (reagents, E. coli strains) for our lesson to the students part of WashU's Pre-Engineering Institute <em>[Zoe, Mark, Maddie, Micah, Alex]</em> </li> | ||
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li> Identified composite part in registry (BBa_J04500) that already consists of BBa_B0034 <em>[Micah, Alex]</em></li> | ||
+ | <li> Planned out presentation and lab for the Pre-Engineering Institute students on June 20 <em>[Micah, Mark, Alex, | ||
+ | Zoe, Maddie]</em></li> | ||
+ | <ul> | ||
+ | <li>Set up lab stations with vortex machines, pipet tips, and micropipettors </li> | ||
+ | </ul> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
− | <p><em></em></p> | + | <p><strong><u>Saturday, June 17</u></strong></p> |
+ | <p><em>Present: Micah</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li>Transformed composite part BBa_J04500 into MHD42 E. coli cells <em>[Micah]</em></li> | |
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
Line 780: | Line 902: | ||
<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
− | <p><em></em></p> | + | <p><strong><u>Sunday, June 18</u></strong></p> |
+ | <p><em>Present: Micah, Mark</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li>Checked the plate of MHD42 cells transformed with BBa_J04500 <em>[Micah]</em></li> | |
+ | <ul> | ||
+ | <li><img src="https://static.igem.org/mediawiki/2017/9/9a/T--WashU_StLouis--2017-06-18_Transformation_of_BBa_J04500_into_MHD42.jpg" style="height:11vw;width:11vw"/></li> | ||
+ | <li>Prepared an overnight culture of the cells transformed with BBa_J04500 <em>[Mark]</em></li> | ||
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
Line 792: | Line 918: | ||
<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
− | <p><em></em></p> | + | <p><strong><u>Monday, June 19</u></strong></p> |
+ | <p><em>Present: Zoe, Mark, Micah, Alex, Maddie, Collin</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li>Miniprepped part BBa_J04500 (lac promoter + RBS) <em>[Maddie,Zoe]</em> </li> | |
+ | <li>Performed restriction digest on parts BBa_J04500 and BBa_E0040 <em>[Maddie, Zoe]</em></li> | ||
+ | <li>Performed gel electrophoresis on parts BBa_J04500 and BBa_E0040 <em>[Maddie, Zoe, Collin]</em></li> | ||
+ | <li>Prepared overnight cultures of 9 E. Coli strains for the Pre-Engineering Institute workshop <em>[Mark, Collin, Micah]</em></li> | ||
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li>Discussed how to build an apparatus to expose cell cultures to UV-B radiation <em>[Alex, Micah, Mark, Collin, | ||
+ | Maddie, Zoe]</em></li> | ||
+ | <li>Researched cyanobacteria transformations <em>[Alex, Micah, Mark, Collin, Maddie, Zoe]</em></li> | ||
+ | <li>Met with Dr. Brennan to give her updates and prepare for the Pre-Engineering Institute event <em>[Alex, Micah, Mark, Collin, Maddie, Zoe]</em></li> | ||
+ | </ol> | ||
</div> | </div> | ||
</div> | </div> | ||
Line 804: | Line 940: | ||
<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
− | <p><em></em></p> | + | <p><strong><u>Tuesday, June 20</u></strong></p> |
+ | <p><em>Present: Micah, Alex, Collin, Zoe, Maddie, Mark</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li>Performed gel purification on parts BBa_J04500 and BBa_E0040 </li> | |
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li>Led a three hour synthetic biology workshop for the WashU Pre-Engineering Institute summer program <em>[Micah, | ||
+ | Mark, Zoe, Maddie, Collin, Alex]</em></li> | ||
+ | <ul> | ||
+ | <li> Presented on the field of synthetic biology and the iTune BioBuilder lab activity </li> | ||
+ | <li> Led the iTune BioBuilder lab activity </li> | ||
+ | </ul> | ||
+ | </ol> | ||
</div> | </div> | ||
</div> | </div> | ||
Line 816: | Line 961: | ||
<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
− | <p><em></em></p> | + | <p><strong><u>Wednesday, June 21</u></strong></p> |
+ | <p><em>Present: Alex, Zoe, Maddie, Collin, Micah, Mark</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li> Ligated BBa_J04500 and BBa_E0040 and transformed the composite plasmid into DH5α E. coli cells <em>[Maddie, Zoe]</em></li> | |
+ | <li> Transformed BBa_I13500 into DH5α E. coli cells <em>[Alex]</em></li> | ||
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li>Purchased materials for the UV-B radiation box <em>[Micah, Collin, Mark]</em></li> | ||
</div> | </div> | ||
</div> | </div> | ||
Line 828: | Line 977: | ||
<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
− | <p><em></em></p> | + | <p><strong><u>Thursday, June 22</u></strong></p> |
+ | <p><em>Present: Alex, Zoe, Maddie, Mark, Micah, Collin</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li>Prepared 50% glycerol stock <em>[Alex, Zoe]</em></li> | |
+ | <li>Prepared six overnight cultures for the DH5α cells transformed with ligated parts BBa_J04500 and BBa_E0040 <em>[Alex, Mark]</em></li> | ||
+ | <li>Prepared overnight culture for the DH5α cells transformed with part BBa_I13500 <em>[Alex, Mark]</em></li> | ||
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li>Purchased more materials for the UV-B radiation box <em>[Micah, Collin, Mark]</em></li> | ||
+ | </ol> | ||
</div> | </div> | ||
</div> | </div> | ||
Line 840: | Line 995: | ||
<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
− | <p><em></em></p> | + | <p><strong><u>Friday, June 23</u></strong></p> |
+ | <p><em>Present: Zoe, Mark, Alex, Maddie, Collin, Micah</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li> Miniprepped the DNA from the six DH5α cultures with BBa_J04500 and BBa_E0040 ligated together <em>[Mark, Zoe]</em></li> | |
+ | <li> Miniprepped the DNA from the DH5α culture with BBa_I13500 <em>[Zoe, Mark]</em></li> | ||
+ | <li> Performed restriction digest on the plasmids consisting of BBa_J04500 and BBa_E0040 ligated together. <em>[Alex, Mark]</em></li> | ||
+ | <li> Performed gel electrophoresis to separate the backbone and the ligated insert (BBa_J04500 and BBa_BBa_E0040) in order to check if the parts were ligated correctly <em>[Mark, Alex]</em></li> | ||
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
Line 852: | Line 1,011: | ||
<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Saturday, June 24</u></strong></p> | ||
+ | <p><em>Present: Micah, Collin, Zoe, Mark</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li>Purchased more materials for the UV exposure box <em>[Micah, Collin, Zoe, Mark]</em></li> | ||
+ | <li>Began the construction of the UV exposure box <em>[Micah, Collin, Zoe, Mark]</em></li> | ||
+ | </ol> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--June 25--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Sunday, June 25</u></strong></p> | ||
<p><em></em></p> | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--June 26--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Monday, June 26</u></strong></p> | ||
+ | <p><em>Present: Zoe, Micah, Collin, Alex, Maddie, Mark</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li>Resuspended the Dsup DNA from IDT <em>[Collin, Micah]</em></li> | |
+ | <li>Digested the Dsup DNA <em>[Collin, Micah]</em></li> | ||
+ | <li>Digested the pSB1A2 plasmids with ligated BBa_J04500 (lac promoter + RBS) and BBa_E0040 (GFP) <em>[Alex, Mark]</em></li> | ||
+ | <li>Performed gel electrophoresis on the digested pSB1A2 plasmids with ligated BBa_J04500 and BBa_E0040 <em>[Alex, Mark]</em></li>. | ||
+ | <ul> | ||
+ | <li><img src="https://static.igem.org/mediawiki/2017/1/1c/T--WashU_StLouis--2017-06-26_Ligation_of_E0040_and_J04500_Test.jpg" style="height:15vw; width:10vw"/></li> | ||
+ | </ul> | ||
+ | <li>Performed gel electrophoresis on the digested Dsup<em>[Collin, Micah]</em></li>. | ||
+ | <ul> | ||
+ | <li><img src="https://static.igem.org/mediawiki/2017/8/8c/T--WashU_StLouis--2017-06-26_Dsup_and_backbone_digestion.jpg" style="height:15vw;width:10vw"/></li> | ||
+ | </ul> | ||
+ | <li>Transformed the pSB1A2 with BBa_J04500 and BBa_E0040 into DH5α E. coli cells <em>[Alex, Maddie]</em></li> | ||
+ | <li>Transformed the pSB3C5 plasmid into DH5α E. coli cells <em>[Maddie]</em></li> | ||
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li>Finished constructing the UV exposure box <em>[Zoe, Collin, Micah]</em></li> | ||
+ | </ol> | ||
</div> | </div> | ||
</div> | </div> | ||
− | <!--June | + | <!--June 27--> |
<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Tuesday, June 27</u></strong></p> | ||
+ | <p><em>Present: Zoe, Mark, Micah, Maddie, Collin, Alex</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Checked for the transformation of DH5α cells with expression plasmid pSB3C5 <em>[Alex]</em></li> | ||
+ | <ul> | ||
+ | <li><img src="https://static.igem.org/mediawiki/2017/8/89/T--WashU_StLouis--2017-06-27_Transormation_of_ligated_E0040_and_J04500.jpg" style="height:11vw;width:11vw"/></li> | ||
+ | </ul> | ||
+ | <li>Checked for the transformation of DH5α cells with the ligated parts, BBa_J04500 (lac promoter + RBS) and BBa_E0040 (GFP) <em>[Alex}</em></li> | ||
+ | <ul> | ||
+ | <li><img src="https://static.igem.org/mediawiki/2017/c/cd/T--WashU_StLouis--2017-06-27_Transormation_of_pSB3C5.jpg" style="height:11vw;width:11vw"/></li> | ||
+ | </ul> | ||
+ | <li>Purified Dsup from its gel <em>[Collin, Micah]</em></li> | ||
+ | <li>Digested the phrA (from Tardigrade species, Ramazzottius varieornatus), phrA (from cyanobacteria), and BBa_R0010 (lac promoter) <em>[Zoe, Maddie]</em></li> | ||
+ | <ul> | ||
+ | <li>We digested BBa_R0010 to obtain its pSB1C3 backbone</li> | ||
+ | </ul> | ||
+ | <li>Performed gel electrophoresis on the digested phrA from tardigrades, phrA from cyanobacteria, and BBa_R0010 (lac promoter) <em>[Mark, Zoe]</em></li> | ||
+ | <ul> | ||
+ | <li><img src="https://static.igem.org/mediawiki/2017/c/c5/T--WashU_StLouis--2017-06-27_phrAT%2C_phrAC%2C_and_pSB1C3_Digestion.jpg" style="height:10vw;width:15vw"/></li> | ||
+ | </ul> | ||
+ | <li>Performed a PCR gradient test on Dsup, phrA (from tardigrades), and phrA (from cyanobacteria) <em>[Maddie, Zoe]</em></li> | ||
+ | <li>Performed gel electrophoresis for the PCR gradient test <em>[Maddie, Zoe]</em></li> | ||
+ | <ul> | ||
+ | <li> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/1/18/T--WashU_StLouis--2017-06-27_DSUP_PCR_gradient.jpg" style="height:10vw;width:15vw"/> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/1/18/T--WashU_StLouis--2017-06-27_phrAT_PCR_gradient.jpg" style="height:10vw;width:15vw"/> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/0/05/T--WashU_StLouis--2017-06-27_phrAC_PCR_gradient.jpg" style="height:10vw;width:15vw"/> | ||
+ | <p><em>left to right:Dsup, phrA (from tardigrades), phrA (from cyanobacteria)</em></p> | ||
+ | </li> | ||
+ | <li>The gel pattern shows that the gradient test failed and that we need to design better primers</li> | ||
+ | </ul> | ||
+ | <li>Evaluated the efficacy of the UV exposure box <em>[Collin, Micah]</em></li> | ||
+ | <ul> | ||
+ | <li>Transformed pSB1C3 with RFP into MHD42 E. coli cells</li> | ||
+ | <li>Plated the transformation on two plates and incubated one in the UV box and the other outside of the box overnight</li> | ||
+ | </ul> | ||
+ | <li>Transformed pSB4C5 into DH5α E. coli cells <em>[Alex]</em></li> | ||
+ | <li>Transformed the ligated BBa_E0040 (GFP), BBa_J04500 (lac promoter + RBS), and pSB1C3 plasmid into DH5α E. coli cells <em>[Alex]</em></li> | ||
+ | <li>Prepared an inoculated overnight culture of DH5α cells with pSB3C5 expression plasmids <em>[Alex]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--June 28--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Wednesday, June 28</u></strong></p> | ||
+ | <p><em>Present: Mark, Alex, Maddie, Zoe, Micah, Collin</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Checked for the transformation of pSB4C5 into DH5α cells <em>[Alex]</em></li> | ||
+ | <ul> | ||
+ | <li><img src="https://static.igem.org/mediawiki/2017/1/1e/T--WashU_StLouis--2017-06-28_Transformation_of_pSB4C5_into_DH5alpha.jpg" style="height:11vw;width:11vw"/></li> | ||
+ | </ul> | ||
+ | <li>Checked for the transformation of the ligated parts, BBa_J04500, BBa_E0040, and pSB1C3, into DH5α cells <em>[Alex]</em></li> | ||
+ | <ul> | ||
+ | <li><img src="https://static.igem.org/mediawiki/2017/9/9f/T--WashU_StLouis--2017-06-28_Transformations_of_ligated_BBa_E0040_and_BBa_J04500.jpg" style="height:11vw;width:11vw"/></li> | ||
+ | </ul> | ||
+ | <li>Miniprepped the pSB3C5 expression plasmids <em>[Zoe]</em></li> | ||
+ | <li>Transformed MHD42 and DH5α E. coli cells with BBa_J04450 (RFP plasmid) to test the efficacy of the UV box <em>[Collin, Micah]</em></li> | ||
+ | <ul> | ||
+ | <li>Placed two plates of the transformation inside the UV box and two plates outside of the box</li> | ||
+ | </ul> | ||
+ | <li>Purified pSB1C3, phrA (from tardigrades), and phrA (from cyanobacteria) from their respective gels <em>[Mark]</em></li> | ||
+ | <li>Ligated Dsup, phrA (from tardigrades), and phrA (from cyanobacteria) into pSB1C3 backbones <em>[Mark, Zoe]</em></li> | ||
+ | <li>Transformed each of the ligations into DH5α E. coli cells <em>[Zoe, Mark]</em></li> | ||
+ | <li>Prepared LB + Chloramphenicol (CM) plates <em>[Mark]</em></li> | ||
+ | |||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li>Worked on modeling growth curves <em>[Micah]</em></li> | ||
+ | </ol> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--June 29--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Thursday, June 29</u></strong></p> | ||
+ | <p><em>Present: Collin, Micah, Maddie, Zoe, Mark, Alex</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Checked on the transformations of Dsup, phrA (from tardigrades), and phrA (from cyanobacteria), all of which were ligated into pSB1C3 plamids previously <em>[Alex]</em></li> | ||
+ | <ul> | ||
+ | <li><img src="https://static.igem.org/mediawiki/2017/5/58/T--WashU_StLouis--2017-06-29_Ligated_Dsup_%2B_pSB1C3.jpg" style="height:11vw;width:11vw"/> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/d/d8/T--WashU_StLouis--2017-06-29_Ligated_phrAT_%2B_pSB1C3.jpg" style="height:11vw;width:11vw"/> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/e/ed/T--WashU_StLouis--2017-06-29_Ligated_phrAC_%2B_pSB1C3.jpg" style="height:11vw;width:11vw"/> | ||
+ | </li> | ||
+ | </ul> | ||
+ | <li>Compared the plates of cells transformed with BBa_J04450 (RFP) that were stored in the UV box with the plates that were stored outside of the box <em>[Micah]</em></li> | ||
+ | <li>Prepared LB Agar <em>[Mark]</em></li> | ||
+ | <li>Prepared overnight cultures of the cells with Dsup, phrA (from tardigrades), phrA (from cyanobacteria), pSB4C5, and the ligated BBa_J04500 and BBa_E0040 (which is lac promoter + RBS + GFP altogether) <em>[Zoe, Alex]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li>Met with Sarah Rommelfanger to discuss our plans of transforming cyanobacteria in the near future <em>[Zoe, Alex, Maddie, Mark, Micah, Collin]</em></li> | ||
+ | <li>Met with Dr. Brennan to update her on our progress <em>[Alex, Mark, Zoe, Maddie, Collin, Micah]</em></li> | ||
+ | </ol> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--June 30--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Friday, June 30</u></strong></p> | ||
<p><em></em></p> | <p><em></em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li>Miniprepped 4 cultures with phrA (from tardigrades), 3 cultures with phrA (from cyanobacteria), 3 cultures with Dsup, 1 culture with the ligated BBa_j04500 and BBa_E0040 (which is lac promoter + RBS + GFP altogether), and 4 cultures with pSB4C5 <em>[Zoe]</em> </li> | |
+ | <li>Digested phrA (from tardigrades), phrA (from cyanobacteria), and Dsup to remove the genes from their pSB1C3 backbones <em>[Zoe]</em></li> | ||
+ | <li>Performed gel electrophoresis on these three digestions <em>[Alex, Collin, Mark]</em></li> | ||
+ | <ul> | ||
+ | <li> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/e/eb/T--WashU_StLouis--2017-06-30_phrAT_digestion.jpg" style="height:10vw;width:15vw"/> | ||
+ | <p><em>phrA from tardigrades (aka phrAT)</em></p> | ||
+ | </li> | ||
+ | <li> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/2/2d/T--WashU_StLouis--2017-06-30_phrAC_%28left_3%29_and_Dsup_%28right_3%29_digestion.jpg" style="height:10vw;width:15vw"/> | ||
+ | <p><em>left 3 lanes: phrA from cyanobacteria (aka phrAC) ; right 3 lanes: Dsup</em></p> | ||
+ | </li> | ||
+ | </ul> | ||
+ | <li>Performed a PCR gradient test on Dsup, phrA (from tardigrades), and phrA (from cyanobacteria) <em>[Maddie, Zoe]</em></li> | ||
+ | <li>Performed gel electrophoresis for the PCR gradient test <em>[Maddie, Zoe, Mark]</em></li> | ||
+ | <ul> | ||
+ | <li> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/a/a1/T--WashU_StLouis--2017-06-30_phrAT_%28top%29_phrAC_%28bot%29_PCR_gradient_%282nd_try%29.jpg" style="height:10vw;width:15vw"/> | ||
+ | <p><em>Top: phrAT ; Bottom: phrAC</em></li> | ||
+ | </li> | ||
+ | <li> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/2/25/T--WashU_StLouis--2017-06-30_Dsup_PCR_gradient_%282nd_try%29.jpg" style="height:10vw;width:15vw"/> | ||
+ | <p><em>Dsup</em></li> | ||
+ | </li> | ||
+ | </ul> | ||
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li>Skyped with Purdue's iGEM team <em>[Micah, Maddie, Mark, Collin, Alex]</em></li> | ||
+ | <li>Worked on the Arduino for the UV exposure box <em>[Micah]</em></li> | ||
+ | <ul> | ||
+ | <li>Configured the temperature and humidity sensing mechanism</li> | ||
+ | </ul> | ||
+ | <li>Worked on the style of the wiki's menu and updated several pages <em>[Mark]</em></li> | ||
</div> | </div> | ||
</div> | </div> | ||
− | <!-- | + | <!--July 1--> |
<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Saturday, July 1</u></strong></p> | ||
<p><em></em></p> | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <!--July 2--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Sunday, July 2</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--July 3--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Monday, July 3</u></strong></p> | ||
+ | <p><em>Present: Micah, Mark, Zoe, Alex</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
+ | <li>Transformed UV promoter on an Amp plate into DH5α cells <em>[Zoe]</em></li> | ||
+ | <li>Transformed Dsup #3, phrAC #1, and phrAT #4 (pSB1C3) into DH5α cells <em>[Zoe}</em></li> | ||
+ | <li>Made LB+CM plates <em>[Mark]</em></li> | ||
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li>Started working on Arduino and designing code for the "To-Grow Box" <em>[Micah, Zoe]</em></li> | ||
+ | </ol> | ||
</div> | </div> | ||
</div> | </div> | ||
− | <!-- | + | <!--July 4--> |
<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
− | <p><em></em></p> | + | <p><strong><u>Tuesday, July 4</u></strong></p> |
+ | <p><em>Present: Alex</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
+ | <li>Checked the transformations of Dsup, phrAT, phrAC, and the UV promoter and moved them to the cold room <em>[Alex]</em></li> | ||
</ol> | </ol> | ||
Line 897: | Line 1,262: | ||
</div> | </div> | ||
− | <!-- | + | <!--July 5--> |
<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
− | <p><em></em></p> | + | <p><strong><u>Wednesday, July 5</u></strong></p> |
+ | <p><em>Present: Mark, Micah, Alex, Maddie, Zoe</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
+ | <li>Transformation of Lac+RBS+GFP (pSB1A2) on Amp plates into DH5α cells <em>[Alex]</em></li> | ||
+ | <li>Prepared 5mL liquid cultures from transformations done on Monday: Dsup#3, phrAC #1, phrAT #4 (all with Cm), and UV promoter (with Amp) <em>[Zoe]</em></li> | ||
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li>Started working on our presentation for our July 10th Monsanto tour <em>[Micah, Maddie, Alex, Mark, Zoe]</em></li> | ||
+ | </ol> | ||
</div> | </div> | ||
</div> | </div> | ||
− | <!-- | + | <!--July 6--> |
<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
− | <p><em></em></p> | + | <p><strong><u>Thursday, July 6</u></strong></p> |
+ | <p><em>Present: Micah, Maddie, Collin, Zoe, Alex</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
+ | <li>Made glycerol stocks of Dsup, phrAT, and phrAC in pSB1C3 from liquid cultures <em>[Alex]</em></li> | ||
+ | <li>Preparation of 21 Amp plates <em>[Alex]</em></li> | ||
+ | <li>Miniprepped UV promoter <em>[Maddie]</em></li> | ||
+ | <li>Transformation of Lac+RBS+GFP (pSB1A2) on Amp plates into DH5α cells <em>[Zoe]</em></li> | ||
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li>Went to hardware store and bought more materials for "To-Grow Box" <em>[Collin, Micah]</em></li> | ||
+ | <li>Designed and started 3D printing parts for the mini shaker for the "To-Grow Box" <em>[Collin, Micah]</em></li> | ||
+ | </ol> | ||
</div> | </div> | ||
</div> | </div> | ||
− | <!-- | + | <!--July 7--> |
<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Friday, July 7</u></strong></p> | ||
+ | <p><em>Present: Micah, Maddie, Collin, Zoe, Alex</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Transformed the UV promoter (BBa_J22106) into DH5α cells and plated them onto a LB + Amp plate <em>[Collin]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li> Talked with the University of Iowa iGEM team over Skype </li> | ||
+ | <li> Worked on the Monsanto presentation </li> | ||
+ | </ol> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--July 8--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Saturday, July 8</u></strong></p> | ||
+ | <p><em>Present: Collin</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Checked on the transformation of the UV promoter (BBa_J22106) <em>[Collin]</em> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--July 9--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Sunday, July 9</u></strong></p> | ||
<p><em></em></p> | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--July 10--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Monday, July 10</u></strong></p> | ||
+ | <p><em>Present: Maddie, Mark, Zoe, Alex, Micah, Collin</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li>Sent Dsup, phrAC, phrAC, and lac promoter+RBS+GFP (all in pSB1C3 plasmids) for sequencing <em>[Alex]</em></li> | |
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li>Visited the Monsanto and Pfizer campus in Chesterfield, MO <em>[Zoe, Collin, Mark, Maddie, Micah, Alex]</em></li> | ||
+ | </ol> | ||
</div> | </div> | ||
</div> | </div> | ||
− | <!--July | + | <!--July 11--> |
<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Tuesday, July 11</u></strong></p> | ||
+ | <p><em>Present: Maddie, Zoe, Alex, Collin, Mark</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Prepared 5 mL liquid culture of BBa_J22106 (UV promoter) and incubated at 37°C <em>[Maddie]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--July 12--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Wednesday, July 12</u></strong></p> | ||
+ | <p><em>Present: Collin, Zoe, Maddie, Alex</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Miniprepped the overnight culture of BBa_J22106 <em>[Maddie]</em></li> | ||
+ | <li>Prepared BG-11 cyanobacteria media <em>[Maddie, Zoe]</em></li> | ||
+ | <li>Measured OD for an undiluted cyanobacteria (S-6803) solution <em>[Maddie, Zoe]</em> | ||
+ | <ul> | ||
+ | <li>The OD was 0.4519</li> | ||
+ | </ul> | ||
+ | <li>Diluted the cyanobacteria culture to an OD of 0.02 in two 250 mL flasks <em>[Zoe, Maddie]</em></li> | ||
+ | <ul> | ||
+ | <li>The actual OD's after the dilution were 0.0207 (#1) and 0.0209 (#2)</li> | ||
+ | <li> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/3/35/T--WashU_StLouis--2017-07-12_Cyano_-1.jpg" style="width:10vw"/> <img src="https://static.igem.org/mediawiki/2017/0/0f/T--WashU_StLouis--2017-07-12_Cyano_-2.jpg" style="width:10vw"/> | ||
+ | <p><em>From left to right: #1, #2</em></p> | ||
+ | </li> | ||
+ | </ul> | ||
+ | <li>Transformed BBa_K592009 (Blue chromoprotein) into DH5α E. coli cells <em>[Alex]</em></li> | ||
+ | <li>Prepared a 20 mL liquid culture of DH5α cells with BBa_J04500 in pSB1C3 <em>[Zoe]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li>Skyped with the Peshawar iGEM team <em>[Maddie, Alex, Collin]</em></li> | ||
+ | </ol> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--July 13--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Thursday, July 13</u></strong></p> | ||
+ | <p><em>Present: Zoe, Mark, Collin, Alex, Maddie</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Prepared 1600 mL of LB Agar <em>[Maddie, Zoe]</em></li> | ||
+ | <li>Prepared 112 small LB+CM plates <em>[Maddie, Mark, Zoe]</em></li> | ||
+ | <li>Measured the OD of the two cyanobacteria cultures <em>[Zoe]</em></li> | ||
+ | <ul> | ||
+ | <li> 0.0443 (#1), 0.1135 (#2)</li> | ||
+ | <li> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/7/71/T--WashU_StLouis--2017-07-13_Cyano_-1.jpg" style="width:10vw"/> <img src="https://static.igem.org/mediawiki/2017/a/a3/T--WashU_StLouis--2017-07-13_Cyano_-2.jpg"style="width:10vw"/> | ||
+ | <p><em>From left to right: #1, #2</em></p> | ||
+ | </li> | ||
+ | </ul> | ||
+ | <li>Transformed BBa_J04500 (Lac promoter + RBS) in DH5α cells <em>[Collin]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--July 14--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Friday, July 14</u></strong></p> | ||
+ | <p><em>Present: Zoe, Collin, Alex, Maddie, Mark</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Prepared 800 mL of LB Agar <em>[Mark]</em></li> | ||
+ | <li>Prepared 59 small LB+CM plates <em>[Maddie, Mark, Zoe]</em></li> | ||
+ | <li>Measured the OD of the two cyanobacteria cultures <em>[Zoe]</em></li> | ||
+ | <ul> | ||
+ | <li> 0.0734 (#1), 0.4439 (#2)</li> | ||
+ | <li> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/5/54/T--WashU_StLouis--2017-07-14_Cyano_-1.jpg" style="width:10vw"/> <img src="https://static.igem.org/mediawiki/2017/8/8b/T--WashU_StLouis--2017-07-14_Cyano_-2.jpg" style="width:10vw"/> | ||
+ | <p><em>From left to right: #1, #2</em></p> | ||
+ | </li> | ||
+ | </ul> | ||
+ | <li>Prepared two more 50 mL cultures of Synechocystis S-6803 and incubated them in the 30°C room <em>[Maddie, Zoe]</em></li> | ||
+ | <ul> | ||
+ | <li>Starting OD's: 0.0213 <#3), 0.0213 (#4)</li> | ||
+ | </ul> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--July 15--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Saturday, July 15</u></strong></p> | ||
+ | <p><em>Present:Zoe</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Measured the OD of all four cyanobacteria cultures <em>[Zoe]</em></li> | ||
+ | <ul> | ||
+ | <li> 0.1179 (#1), 0.9077 (#2), 0.0592 (#3), 0.0436 (#4)</li> | ||
+ | <li> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/e/ef/T--WashU_StLouis--2017-07-15_Cyano_-3.jpg" style="width:10vw"/><img src="https://static.igem.org/mediawiki/2017/7/71/T--WashU_StLouis--2017-07-15_Cyano_-4.jpg" style="width:10vw"/> | ||
+ | <p><em>From left to right: #1, #2 #3, #4</em></p> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--July 16--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Sunday, July 16</u></strong></p> | ||
+ | <p><em>Present:Zoe</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Measured the OD of the four cyanobacteria cultures <em>[Zoe]</em></li> | ||
+ | <ul> | ||
+ | <li> 0.1791 (#1), 1.3581 (#2), 0.1312 (#3), 0.0799 (#4)</li> | ||
+ | </ul> | ||
+ | <li>Prepared liquid cultures of lac promoter + RBS (BBa_J04500) and Blue chromoprotein (BBa_K592009) <em>[Zoe]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--July 17--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Monday, July 17</u></strong></p> | ||
+ | <p><em>Present: Mark, Zoe, Micah, Collin, Alex, Maddie</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Miniprepped 4 E. coli cultures with lac promoter + RBS (BBa_J04500) <em>[Zoe]</em></li> | ||
+ | <li>Miniprepped 1 E. coli culture with blue chromoprotein (BBa_K592009) <em>[Zoe]</em></li> | ||
+ | <li>Digested Dsup, phrAC, phrAT, uvsE, RBS (BBa_B0034), Lac promoter + RBS (BBa_J04500), and Blue Chromoprotein (BBa_K592009) <em>[Collin]</em></li> | ||
+ | <li>Performed gel electrophoresis on the digested Dsup, phrAC, phrAT, uvsE, RBS, Lac promoter + RBS, and blue chromoprotein <em>[Mark, Collin]</em></li> | ||
+ | <li>Measured the OD730 of the 4 cyanobacteria cultures <em>[Zoe]</em></li> | ||
+ | <ul> | ||
+ | <li> 0.2854 (#1), 2.4509 (#2), 0.2725 (#3), 0.1262 (#4)</li> | ||
+ | </ul> | ||
+ | <li>Streaked the 9 strains of E. coli from the BioBuilder iTUNE activity for the Pre-Engineering Institute workshop <em>[Mark, Collin]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li>Skyped with Austin Burns, the Regulatory Manager at Monsanto <em>[Alex]</em></li> | ||
+ | <li>Worked on the code for the Environmental Simulation System (ESS) <em>[Micah]</em></li> | ||
+ | </ol> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--July 18--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Tuesday, July 18</u></strong></p> | ||
+ | <p><em>Present: Maddie, Zoe, Mark, Collin, Alex, Micah</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Purified uvsE, phrAT, phrAC, Dsup, Lac promoter + RBS, RBS, and blue chromoprotein from their respective gels <em>[Maddie]</em></li> | ||
+ | <li>Ligated uvsE to lac + RBS, phrAT to lac + RBS, phrAC to lac + RBS, Dsup to lac + RBS, and RBS to blue chromoprotein <em>[Maddie]</em></li> | ||
+ | <li>Transformed the ligated plasmids into DH5α E. coli cells <em>[Maddie]</em></li> | ||
+ | <li>Measured the OD730 of the 4 cyanobacteria cultures <em>[Zoe, Mark]</em></li> | ||
+ | <ul> | ||
+ | <li>0.4047 (#1), 3.2044 (#2), 0.4633 (#3), 0.1951 (#4)</li> | ||
+ | </ul> | ||
+ | <li>Prepared overnight cultures of the 9 strains of E. coli from the Biobuilder iTune activity (for the Pre-Engineering Institute workshop <em>[Alex]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li>Worked on the Environmental Simulation System (ESS) <em>[Micah, Collin]</em></li> | ||
+ | <li>Prepared materials for the Pre-Engineering Institute workshop <em>[Mark, Maddie, Alex, Micah]</em></li> | ||
+ | </ol> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--July 19--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Wednesday, July 19</u></strong></p> | ||
+ | <p><em>Present: Micah, Collin, Maddie, Mark, Zoe, Alex</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Checked the transformations of lac+RBS+Dsup, lac+RBS+phrAT, lac+RBS+phrAC, lac+RBS+uvsE, and RBS+blue chromoprotein <em>[Alex]</em></li> | ||
+ | <li>Measured the OD730 of the 4 cyanobacteria cultures <em>[Zoe]</em></li> | ||
+ | <ul> | ||
+ | <li> 0.5425 (#1), 3.6448 (#2), 0.7760 (#3), 0.3022 (#4)</li> | ||
+ | </ul> | ||
+ | <li>Transforomed lac+RBS+phrAT into DH5α E. coli cells (again) <em>[Maddie]</em></li> | ||
+ | <li>Prepared overnight cultures for 3 colonies each of lac+RBS+phrAT, lac+RBS+phrAC, lac+RBS+Dsup, lac+RBS+uvsE, and RBS+Blue chromoprotein <em>[Zoe]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li>Led a synthetic biology workshop for high school students in the Pre-Engineering Institute Program <em>[Mark, Collin, Zoe, Alex, Maddie, Micah]</em></li> | ||
+ | <li>Worked on the Environmental Simulation System <em>[Micah]</em></li> | ||
+ | </ol> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--July 20--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Thursday, July 20</u></strong></p> | ||
+ | <p><em>Present:Micah, Alex, Collin, Maddie, Mark, Zoe</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Prepared LB+CM plates <em>[Mark]</em></li> | ||
+ | <li>Prepared glycerol stocks of lac+RBS+phrAT, lac+RBS+phrAC, lac+RBS+Dsup, lac+RBS+uvsE, and RBS+blue chromoprotein <em>[Alex]</em></li> | ||
+ | <li>Miniprepped each of the overnight cultures of DH5α E. coli cells with lac+RBS+phrAT, lac+RBS+phrAC, lac+RBS+Dsup, lac+RBS+uvsE, and RBS+blue chromoprotein <em>[Alex]</em></li> | ||
+ | <li>Digested the miniprepped lac+RBS+phrAT, lac+RBS+phrAC, lac+RBS+Dsup, lac+RBS+uvsE, and RBS+blue chromoprotein <em>[Mark, Collin]</em></li> | ||
+ | <li>Measured the OD730 of the 4 cyanobacteria cultures <em>[Collin, Mark]</em></li> | ||
+ | <ul> | ||
+ | <li> 0.7062 (#1), 3.9385 (#2), 1.0866 (#3), 0.7098 (#4)</li> | ||
+ | </ul> | ||
+ | <li>Performed gel electrophoresis on the digested lac+RBS+phrAT, lac+RBS+phrAC, lac+RBS+Dsup, lac+RBS+uvsE, and RBS+blue chromoprotein to test if the ligation was correct <em>[Maddie, Zoe]</em></li> | ||
+ | <li>Prepared 3 overnight cultures of DH5α E. coli cells with lac+RBS+phrAT <em>[Alex]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li>Presented on our project and the iGEM competition as a whole to high school teachers attending the BioBuilder Professional Development Workshop at Monsanto <em>[Maddie, Zoe, Mark, Collin, Micah]</em></li> | ||
+ | </ol> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--July 21--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Friday, July 21</u></strong></p> | ||
+ | <p><em>Present: Alex, Collin, Zoe, Maddie, Micah, Mark</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Miniprepped the three cultures with lac+RBS+phrAT <em>Alex</em></li> | ||
+ | <li>Measured the OD730 of the four cyanobacteria cultures <em>[Maddie]</em></li> | ||
+ | <ul> | ||
+ | <li>0.9366 (#1), 3.989 (#2), 1.584 (#3), 0.6518 (#4)</li> | ||
+ | </ul> | ||
+ | <li>Measured the amount of light that each cyanobacteria is receiving on average (in micro-molars per meter squared per second) using a FieldScout Quantum Light Meter <em>[Maddie, Zoe]</em></li> | ||
+ | <ul> | ||
+ | <li> 8.4 (#1), 23 (#2), 16.6 (#3), 9.8 (#4)</li> | ||
+ | </ul> | ||
+ | <li>Digested lac+RBS+uvsE, lac+RBS+phrAC, lac+RBS+Dsup, and RBS+blue chromoprotein to determine if the ligations were correct <em>[Alex, Mark]</em></li> | ||
+ | <li>Performed gel electrophoresis on the 3 miniprepped samples (#4, #5, #6) of phrAT and the digested lac+RBS+uvsE, lac+RBS+phrAC, lac+RBS+Dsup, and RBS+blue chromoprotein to test if the ligations were correct <em>[Alex]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li>Skyped with Cardiff iGEM to explore potential collaboration scenarios <em>[Alex, Micah, Mark, Zoe, Collin]</em></li> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--July 22--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Saturday, July 22</u></strong></p> | ||
<p><em></em></p> | <p><em></em></p> | ||
+ | <p>No lab work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--July 23--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Sunday, July 23</u></strong></p> | ||
+ | <p><em>Present: Maddie</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li>Measured the OD730 of the four cyanobacteria cultures <em>[Maddie]</em></li> | |
+ | <ul> | ||
+ | <li> 1.4122 (#1), 3.965 (#2), 2.3244 (#3), 0.9878 (#4)</li> | ||
+ | </ul> | ||
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
Line 945: | Line 1,633: | ||
</div> | </div> | ||
− | <!--July | + | <!--July 24--> |
<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Monday, July 24</u></strong></p> | ||
+ | <p><em>Present: Maddie, Micah, Mark, Zoe, Collin, Alex</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Digested lac+RBS+uvsE, lac+RBS+phrAC, lac+RBS+Dsup, phrAT, lac+RBS and RBS+blue chromoprotein <em>[Alex]</em></li> | ||
+ | <li>Performed gel electrophoresis for the digested lac+RBS+uvsE, lac+RBS+phrAC, lac+RBS+Dsup, phrAT, lac+RBS and RBS+blue chromoprotein <em>[Alex]</em></li> | ||
+ | <li>Measured the OD730 of the four cyanobacteria cultures <em>[Mark, Micah]</em></li> | ||
+ | <ul> | ||
+ | <li> 1.7252 (#1), 3.884 (#2), 2.8400 (#3), 1.218 (#4)</li> | ||
+ | </ul> | ||
+ | <li>Digested uvsE, phrAC, Dsup, and lac+RBS <em>[Collin, Mark]</em></li> | ||
+ | <li>Performed gel electrophoresis on digested uvsE, phrAC, Dsup, and lac+RBS <em>[Mark, Collin]</em></li> | ||
+ | <li>Performed a PCR gradient test on phrAT, phrAC, Dsup, and uvsE with Golden Gate compatible primers <em>[Maddie, Zoe]</em></li> | ||
+ | <ul> | ||
+ | <li>uvsE and Dsup were successful</li> | ||
+ | </ul> | ||
+ | <li>Prepared three overnight cultures of lac+RBS+phrAC, three cultures of lac+RBS+uvsE, and two cultures of lac+RBS+Dsup <em>[Zoe]</em></li> | ||
+ | |||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li>Practiced our presentation for the EECE poster session <em>[Maddie, Alex, Collin]</em></li> | ||
+ | <li>Met with Dr. Brennan to discuss our progress <em>[Mark, Maddie, Alex, Collin, Zoe, Micah]</em></li> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--July 25--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Tuesday, July 25</u></strong></p> | ||
+ | <p><em>Present: Micah, Alex, Collin, Zoe, Mark, Maddie</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Made glycerol stocks of three cultures with lac+RBS+phrAC (#4, #5, #6), three cultures with lac+RBS+uvsE (#4, #5, #6), and two cultures with lac+RBS+Dsup (#4, #5) <em>[Alex]</em></li> | ||
+ | <li>Performed gel purification on the Golden Gate (GG) compatible uvsE and Dsup PCR products <em>[Maddie, Mark]</em></li> | ||
+ | <li>Performed gel purification on lac+RBS, phrAC, Dscup, and uvsE <em>[Mark, Maddie]</em></li> | ||
+ | <li>Miniprepped three cultures with lac+RBS+phrAC (#4, #5, #6), three cultures with lac+RBS+uvsE (#4, #5, #6), and two cultures with lac+RBS+Dsup (#4, #5) <em>[Zoe]</em></li> | ||
+ | <li>Measured the OD730 of the four cyanobacteria colonies <em>[Mark, Collin]</em></li> | ||
+ | <ul> | ||
+ | <li> 2.126 (#1), 3.881 (#2), 3.667 (#3), 1.5272 (#4)</li> | ||
+ | </ul> | ||
+ | <li>Transformed BBa_I13521 (RFP with a constitutive promoter) into DH5α E. coli cells <em>[Maddie]</em></li> | ||
+ | <li>Ligated lac+RBS (BBa_J04500) with phrAC, Dsup, phrAT, and uvsE individually in pSB1C3 <em>[Alex, Mark, Collin]</em> | ||
+ | <li>Transformed the ligated lac+RBS+Dsup, lac+RBS+phrAT, lac+RBS+phrAC, and lac+RBS+uvsE <em>[Mark, Collin]</em></li> | ||
+ | |||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li>Presented at the EECE summer poster session <em>[Zoe, Maddie, Collin, Alex, Micah, Mark]</em></li> | ||
+ | </ol> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--July 26--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Wednesday, July 26</u></strong></p> | ||
+ | <p><em>Present: Maddie, Mark Wang, Collin, Alex, Zoe, Micah</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Measured the OD730 of the four cyanobacteria cultures <em>[Collin, Mark]</em></li> | ||
+ | <ul> | ||
+ | <li> 2.2204 (#1), 3.742 (#2), 3.786 (#3), 1.7132 (#4) | ||
+ | </ul> | ||
+ | <li>Performed gradient PCR on PCV0020 <em>[Zoe, Maddie]</em></li> | ||
+ | <ul> | ||
+ | <li> Did not work </li> | ||
+ | </ul> | ||
+ | <li>Made liquid cultures for 3 colonies each of lac+RBS+uvsE, lac+RBS+Dsup, lac+RBS+phrAC, lac+RBS+phrAT (#7, #8, #9) <em>[Alex]</em></li> | ||
+ | <li>Tested the efficacy of the UV box by exposing plates of DH5α E. coli cells with RFP to UV for different time intervals <em>[Micah, Mark]</em></li> | ||
+ | <li>Plated pDeg+PCPC360 and pDeg+pTrc on Kan plates and PCV0020 on Spec plates <em>[Collin, Mark]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li>Met with Dr. Larry Gilbertson, a molecular biologist at Monsanto, and discussed methods of transforming plant cells <em>[Alex, Micah, Mark, Collin, Zoe]</em></li> | ||
+ | </ol> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--July 27--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Thursday, July 27</u></strong></p> | ||
+ | <p><em>Present: Zoe, Micah, Alex, Collin, Mark</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Checked the UV-exposed RFP plates <em>[Micah]</em></li> | ||
+ | <li>Made glycerol stocks for three cultures each of lac+RBS+phrAC, lac+RBS+phrAT, lac+RBS+uvsE, and lac+RBS+Dsup (#7, #8, #9) <em>[Alex]</em></li> | ||
+ | <li>Miniprepped the three cultures each of lac+RBS+phrAC, lac+RBS+phrAT, lac+RBS+uvsE, and lac+RBS+Dsup (#7, #8, #9) <em>[Zoe, Mark]</em> </li> | ||
+ | <li>Prepared Kanamycin (Kan) and Spectinomycin (Spec) solutions <em>[Mark, Collin]</em></li> | ||
+ | <li>Measured the OD730 of the four cyanobacteria cultures <em>[Mark]</em></li> | ||
+ | <ul> | ||
+ | <li> 2.4388 (#1), 0.3345 (#2), 0.3613 (#3), 1.9760 (#4)</li> | ||
+ | </ul> | ||
+ | <li>Tested the UV box and shaker with two cultures of DH5α E. coli cells with RFP (One inside the box and the other outside the box) <em>[Micah]</em></li> | ||
+ | <li>Prepared three overnight cultures of DH5α E. coli cells with pDeg+PCPC360, pDeg+pTrc, and PCV0020 <em>[Collin]</em></li> | ||
+ | <li>Digested lac+RBS+Dsup #7, lac+RBS+uvsE #7, lac+RBS+phrAC #8, lac+RBS+phrAT #9, and RBS+blue chromoprotein #1 <em>[Alex]</em></li> | ||
+ | <li>Performed gel electrophoresis for the digested lac+RBS+Dsup #7, lac+RBS+uvsE #7, lac+RBS+phrAC #8, lac+RBS+phrAT #9, and RBS+blue chromoprotein #1 <em>[Alex]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--July 28--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Friday, July 28</u></strong></p> | ||
+ | <p><em>Present: Collin, Mark, Zoe, Alex, Micah</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Checked the OD600 of the two cultures used to test the UV box and shaker <em>[Micah]</em></li> | ||
+ | <li>Prepared glycerol stocks of DH5α E. coli cells with pDeg+PCPC360, pDeg+pTrc, and PCV0020 <em>[Alex]</em></li> | ||
+ | <li>Miniprepped the DH5α E. coli cells with pDeg+PCPC360, pDeg+pTrc, and PCV0020 <em>[Zoe]</em></li> | ||
+ | <li>Measured the OD730 of the cyanobacteria cultures <em>[Mark, Collin]</em></li> | ||
+ | <ul> | ||
+ | <li>2.8328 (#1), 3.682 (#2), 3.652 (#3), 2.2800 (#4)</li> | ||
+ | </ul> | ||
+ | <li>Performed a PCR gradient for phrAT and phrAC with GG compatible primers <em>[Alex]</em></li> | ||
+ | <li>Performed gel electrophoresis for the PCR gradient for phrAT and phrAC with GG compatible primers <em>[Alex]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--July 29--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Saturday, July 29</u></strong></p> | ||
+ | <p><em>Present: Micah, Collin</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Measured the OD600 of the DH5α E. coli cultures (one inside the box and one in the culture room) for the UV box test <em>[Micah]</em></li> | ||
+ | <li>Measured the OD730 of the four cyanobacteria cultures <em>[Collin]</em></li> | ||
+ | <ul> | ||
+ | <li>3.0176 (#1), 3.622 (#2), 3.468 (#3), 2.2704 (#4)</li> | ||
+ | </ul> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--July 30--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Sunday, July 30</u></strong></p> | ||
<p><em></em></p> | <p><em></em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Measured the OD730 of the four cyanobacteria cultures <em>[Mark]</em></li> | ||
+ | <ul> | ||
+ | <li>3.3316 (#1), 3.489 (#2), 3.314 (#3), 2.7836 (#4)</li> | ||
+ | </ul> | ||
+ | <li>Prepared overnight cultures of DH5α E. coli cells with lac+RBS+Dsup, lac+RBS+phrAT, and RFP individually <em>[Mark]</em></li> | ||
+ | <li>Digested uvsE, phrAT, phrAC, and Dsup from pSB1C3 and digested lac+RBS+psB1A2 to linearize the plasmid <em>[Alex]</em></li> | ||
+ | <li>Performed gel electrophoresis for uvsE, phrAT, phrAC, Dsup, and lac+RBS+psB1A2 <em>[Alex]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--July 31--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Monday, July 31</u></strong></p> | ||
+ | <p><em>Present: Collin, Micah, Alex, Mark, Zoe</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Performed a PCR gradient for phrAT and phrAC with GG compatible primers <em>[Mark, Collin]</em></li> | ||
+ | <li>Performed gel electrophoresis for the PCR gradient of phrAT and phrAC (with GG compatible primers) <em>[Mark]</em></li> | ||
+ | <li>Measured the OD730 of the four cyanobacteria cultures <em>[Mark]</em></li> | ||
+ | <ul> | ||
+ | <li>3.913 (#1), 3.371 (#2), 3.357 (#3), 3.273 (#4)</li> | ||
+ | </ul> | ||
+ | <li>Prepared a liquid culture of DH5α cells wih lac+RBS+phrAT in pSB1C3 <em>[Zoe]</em></li> | ||
+ | <li>Ligated phrAT, Dsup, phrAC, and uvsE into a pSB1A2 backbone with lac+RBS <em>[Zoe]</em></li> | ||
+ | <li>Performed another PCR gradient for phrAT with GG compatible ends <em>[Collin]</em></li> | ||
+ | <li>Performed gel electrophoresis for the PCR gradient of phrAT with GG compatible ends <em>[Collin, Mark]</em></li> | ||
+ | <li>Digested lac+RBS+Dsup+pSB1C3 and lac+RBS+phrAT+pSB1C3 to linearize the plasmids <em>[Micah]</em></li> | ||
+ | <li>Performed gel electrophoresis on the digested lac+RBS+Dsup+pSB1C3 and lac+RBS+phrAT+pSB1C3 plasmids <em>[Mark]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 1--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Tuesday, August 1</u></strong></p> | ||
+ | <p><em>Present: Zoe, Mark, Micah, Collin, Alex</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Miniprepped lac+RBS+phrAT+pSB1C3 <em>[Zoe]</em></li> | ||
+ | <li>Purified RBS+blue chromoprotein and the linearized lac+RBS+Dsup+pSB1C3 from their respective gels <em>[Mark]</em></li> | ||
+ | <li>Digested PCPC360 and pTrc to linearize both plasmids <em>[Micah]</em></li> | ||
+ | <li>Performed gel electrophoresis on the digested PCPC360 and pTrc <em>[Micah, Mark]</em></li> | ||
+ | <li>Digested lac+RBS+phrAT+pSB1C3 <em>[Micah]</em></li> | ||
+ | <li>Performed gel electrophoresis on the digested lac+RBS+phrAT+pSB1C3 along with undigested PCPC360 and pTrc <em>[Mark]</em></li> | ||
+ | <li>Measured the OD730 of the four cyanobacteria cultures <em>[Micah]</em></li> | ||
+ | <ul> | ||
+ | <li>4.078 (#1), 3.298 (#2), 3.163 (#3), 3.422 (#4)</li> | ||
+ | </ul> | ||
+ | <li>Ligated RBS+Blue chromoprotein into lac+RBS+Dsup+pSB1C3 <em>[Mark]</em></li> | ||
+ | <li>Transformed the ligated lac+RBS+Dsup+RBS+Blue chromoprotein+psB1C3 into DH5α E. coli cells <em>[Mark, Zoe]</em></li> | ||
+ | <li>Prepared three cultures each of DH5α E. coli cells with lac+RBS+uvsE+psB1A2, lac+RBS+Dsup+psB1A2, lac+RBS+phrAT+pSB1A2, and lac+RBS+phrAC+pSB1A2 (numbered #1, #2, #3) <em>[Micah]</em></li> | ||
+ | <li>Prepared an overnight cultures of DH5α E. coli cells <em>[Collin]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 2--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Wednesday, August 2</u></strong></p> | ||
+ | <p><em>Present: Zoe, Mark, Micah, Collin, Alex</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Prepared glycerol stocks for each culture of DH5α E. coli cells with lac+RBS+uvsE+psB1A2, lac+RBS+Dsup+psB1A2, lac+RBS+phrAT+pSB1A2, and lac+RBS+phrAC+pSB1A2 (numbered #1, #2, #3) <em>[Mark]</em></li> | ||
+ | <li>Miniprepped each culture of DH5α E. coli cells with lac+RBS+uvsE+psB1A2, lac+RBS+Dsup+psB1A2, lac+RBS+phrAT+pSB1A2, and lac+RBS+phrAC+pSB1A2 (numbered #1, #2, #3) <em>[Zoe]</em></li> | ||
+ | <li>Measured the OD730 of the four cyanobacteria cultures <em>[Mark]</em></li> | ||
+ | <ul> | ||
+ | <li>4.783 (#1), 3.230 (#2), 3.110 (#3), 4.019 (#4)</li> | ||
+ | </ul> | ||
+ | <li>Prepared an overnight culture of a DH5α E. coli colony with ligated lac+RBS+Dsup+RBS+blue chromoprotein+pSB1C3 <em>[Micah]</em></li> | ||
+ | <li>Digested the previously ligated lac+RBS+phrAT+pSB1A2, lac+RBS+Dsup+pSB1A2, lac+RBS+phrAC+pSB1A2, and lac+RBS+uvsE+pSB1A2 to test if the ligations were correct <em>[Mark, Micah]</em></li> | ||
+ | <li>Performed gel electrophoresis on the digested lac+RBS+phrAT+pSB1A2, lac+RBS+Dsup+pSB1A2, lac+RBS+phrAC+pSB1A2, and lac+RBS+uvsE+pSB1A2 <em>[Mark]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 3--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Thursday, August 3</u></strong></p> | ||
+ | <p><em>Present: Zoe, Mark, Micah, Collin, Alex</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Miniprepped the lac+RBS+Dsup+RBS+blue chromoprotein+pSB1C3 <em>[Zoe]</em></li> | ||
+ | <li>Digested the pCB3C5 backbone, lac+RBS+Dsup+pSB1A2, lac+RBS+phrAT+pSB1A2, lac+RBS+phrAC+pSB1A2, lac+RBS+uvsE+pSB1A2, and RBS+Blue chromoprotein <em>[Collin]</em></li> | ||
+ | <li>Measured the OD730 of the four cyanobacteria cultures <em>[Mark]</em></li> | ||
+ | <ul> | ||
+ | <li>5.052 (#1), 3.208 (#2), 3.156 (#3), 4.308 (#4)</li> | ||
+ | </ul> | ||
+ | <li>Performed gel electrophoresis on the digested pCB3C5 backbone, lac+RBS+Dsup+pSB1A2, lac+RBS+phrAT+pSB1A2, lac+RBS+phrAC+pSB1A2, lac+RBS+uvsE+pSB1A2, and RBS+Blue chromoprotein <em>[Mark, Collin]</em></li> | ||
+ | <li>Digested the previously ligated lac+RBS+Dsup+RBS+blue chromoprotein+pSB1C3 to test if the ligation was correct<em>[Alex]</em></li> | ||
+ | <li>Performed gel electrophoresis on the digested lac+RBS+Dsup+RBS+blue chromoprotein+pSB1C3 <em>[Alex]</em></li> | ||
+ | <li>Prepared three overnight cultures of DH5α E. coli cells with lac+RBS+Dsup+RBS+blue chromoprotein+pSB1C3 and with lac+RBS+phrAT+pSB1A2 <em>[Micah]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 4--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Friday, August 4</u></strong></p> | ||
+ | <p><em>Present: Zoe, Mark, Micah, Collin, Alex</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Miniprepped three cultures of lac+RBS+Dsup+RBS+blue chromoprotein+pSB1C3 (#2, #3, #4) and three cultures of lac+RBS+phrAT+pSB1A2 (#1, #2, #3) <em>[Zoe]</em></li> | ||
+ | <li>Measured the OD730 of the four cyanobacteria cultures <em>[Mark]</em></li> | ||
+ | <ul> | ||
+ | <li>5.207 (#1), 3.122 (#2), 3.024 (#3), 4.709 (#4)</li> | ||
+ | </ul> | ||
+ | <li>Prepared overnight cultures of DH5α E. coli cells with lac+RBS+uvsE+pSB1A2 and lac+RBS+phrAC+pSB1A2 <em>[Mark]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 5--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Saturday, August 5</u></strong></p> | ||
+ | <p><em>Present: Zoe, Mark, Micah, Collin, Alex</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Measured the OD730 of the four cyanobacteria cultures <em>[Collin]</em></li> | ||
+ | <ul> | ||
+ | <li>7.436 (#1), 3.031 (#2), 2.914 (#3), 4.865 (#4)</li> | ||
+ | </ul> | ||
+ | <li>Miniprepped lac+RBS+uvsE+pSB1A2 and lac+RBS+phrAC+pSB1A2 <em>[Collin]</em></li> | ||
+ | <li>Plated DH5α E. coli cells with lac+RBS+Dsup+RBS+Blue chromoprotein+pSB1C3 from its glycerol stock <em>[Collin]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 6--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Sunday, August 6</u></strong></p> | ||
+ | <p><em>Present: Zoe, Mark, Micah, Collin, Alex</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Measured the OD730 of the four cyanobacteria cultures <em>[Mark]</em></li> | ||
+ | <ul> | ||
+ | <li>5.473 (#1), 3.029 (#2), 2.650 (#3), 4.676 (#4)</li> | ||
+ | </ul> | ||
+ | <li>Prepared an overnight cultures from the glycerol stock of DH5α E. coli cells with lac+RBS+Dsup+RBS+blue chromoprotein+pSB1C3 <em>[Mark]</em></li> | ||
+ | <li>Digested lac+RBS+phrAC+pSB1A2 (3 samples: #4, #5, and #6), lac+RBS+uvsE+pSB1A2 (3 samples: #4, #5, and #6), lac+RBS+pSB1A2, phrAT+pSB1C3, phrAC+pSB1C3, uvsE+psB1C3, lac+RBS+Dsup+RBS+blue chromoprotein+pSB1C3, pSB4C5, and pSB3C5 <em>[Collin, Mark]</em></li> | ||
+ | <li>Performed gel electrophoresis on the digested lac+RBS+phrAC+pSB1A2 (3 samples: #4, #5, and #6), lac+RBS+uvsE+pSB1A2 (3 samples: #4, #5, and #6), lac+RBS+pSB1A2, phrAT+pSB1C3, phrAC+pSB1C3, uvsE+psB1C3, lac+RBS+Dsup+RBS+blue chromoprotein+pSB1C3, pSB4C5, and pSB3C5 <em>[Collin]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 7--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Monday, August 7</u></strong></p> | ||
+ | <p><em>Present: Zoe, Mark, Micah, Maddie, Collin, Alex</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Measured the OD730 of the four cyanobacteria cultures <em>[Maddie]</em></li> | ||
+ | <ul> | ||
+ | <li>5.820 (#1), 2.935 (#2), 2.564 (#3), 4.845 (#4)</li> | ||
+ | </ul> | ||
+ | <li>Purified the digested uvsE, phrAC, lac+RBS+psB1A2, pSB4C5, and phrAT from their respective agarose gels <em>[Collin]</em></li> | ||
+ | <li>Miniprepped lac+RBS+Dsup+RBS+blue chromoprotein+pSB1C3 <em>[Micah]</em></li> | ||
+ | <li>Prepared competent DH5α E. coli cells <em>[Micah, Maddie]</em></li> | ||
+ | <li>Transformed DH5α E. coli cells with RFP (BBa_J00450) <em>[Maddie, Micah]</em></li> | ||
+ | <li>Ligated uvsE to lac+RBS+pSB1A2, phrAC to lac+RBS+pSB1A2, and lac+RBS+Dsup+RBS+blue chromoprotein to pSB4C5 <em>[Collin]</em></li> | ||
+ | <li>Transformed DH5α E. coli cells with the ligated lac+RBS+pSB1A2, lac+RBS+phrAC+pSB1A2, and lac+RBS+Dsup+RBS+blue chromoprotein+pSB4C5 <em>[Collin]</em></li> | ||
+ | <li>Digested phrAT+pSB1C3, lac+RBS+uvsE+pSB1A2 #5, pSB1C3+RBS+blue chromoprotein, and lac+pSB1C3 <em>[Mark]</em></li> | ||
+ | <li>Performed gel electrophoresis on the digested phrAT+pSB1C3, lac+RBS+uvsE+pSB1A2 #5, pSB1C3+RBS+blue chromoprotein, and lac+pSB1C3 <em>[Mark]</em></li> | ||
+ | <li>Prepared LB Media <em>[Maddie]</em></li> | ||
+ | <li>Performed the UV irradiation test on DH5α cells with Dsup (with time intervals of 0, 2, 5, and 15 minutes) <em>[Micah, Alex]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 8--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Tuesday, August 8</u></strong></p> | ||
+ | <p><em>Present: Zoe, Mark, Micah, Maddie, Collin, Alex</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Purified lac+RBS+uvsE+pSB1A2, RBS+blue chromoprotein, lac+pSB1C3, and lac+RBS+uvsE from their respective agarose gels <em>[Collin]</em></li> | ||
+ | <li>Performed PCR on phrAT with Golden Gate (GG) compatible primers (Step 1) <em>[Maddie]</em></li> | ||
+ | <li>Performed gel electrophoresis on the GG compatible phrAT PCR product <em>[Maddie]</em></li> | ||
+ | <li>Purified the GG compatible phrAT PCR product from its agarose gel <em>[Maddie]</em></li> | ||
+ | <li>Measured the OD730 of two cyanobacteria cultures <em>[Mark]</em></li> | ||
+ | <ul> | ||
+ | <li>6.465 (#1), 4.660 (#4)</li> | ||
+ | <li>We decided to only continue measuring the the OD730 of growing cultures. Cultures #2 and #3 seem to have already plateaued</li> | ||
+ | </ul> | ||
+ | <li>Performed Golden Gate assembly to ligate Dsup to pDeg_pTrc, uvsE to pDeg_pTrc, Dsup to pDeg_PCPC, and uvsE to pDeg_PCPC <em>[Alex]</em> </li> | ||
+ | <li>Ligated phrAT to lac+RBS+pSB1A2, RBS+blue chromoprotein to lac+RBS+Dsup+pSB1A2, RBS+blue chromoprotein to pSB1C3+lac+RBS+uvsE, and lac+RBS+uvsE to pSB4C5 <em>[Mark]</em></li> | ||
+ | <li>Transformed DH5α E. coli cells with the ligated lac+RBS+phrAT+pSB1A2, lac+RBS+Dsup+RBS+blue chromoprotein+pSB1A2, lac+RBS+uvsE+RBS+blue chromoprotein+pSB1C3, and lac+RBS+uvsE+pSB4C5<em>[Mark, Zoe]</em> | ||
+ | <li>Performed the UV irradiation test on DH5α cells with Dsup (with time intervals of 0, 15, 30, and 60 minutes) <em>[Micah, Mark]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 9--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Wednesday, August 9</u></strong></p> | ||
+ | <p><em>Present: Zoe, Mark, Micah, Maddie, Collin, Alex</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Measured the OD730 of two cyanobacteria cultures <em>[Collin]</em></li> | ||
+ | <ul> | ||
+ | <li>6.434 (#1), 4.676 (#4)</li> | ||
+ | </ul> | ||
+ | <li>Ligated phrAT to lac+RBS+pSB1A2, RBS+blue chromoprotein to lac+RBS+uvsE+pSB1A2, phrAC to lac+RBS+pSB1A2, and uvsE to lac+RBS+pSB1A2 <em>[Collin]</em></li> | ||
+ | <li>Transformed lac+RBS+phrAT+pSB1A2, lac+RBS+uvsE+RBS+blue chromoprotein+pSB1A2, lac+RBS+phrAC+pSB1A2, and lac+RBS+uvsE+pSB1A2 into DH5α E. coli cells <em>[Collin, Zoe]</em></li> | ||
+ | <li>Performed the UV irradiation test on DH5α cells with Dsup (with time intervals of 0, 30, 60, 90, and 120 minutes) <em>[Micah, Mark, Collin, Zoe, Maddie]</em></li> | ||
+ | |||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 10--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Thursday, August 10</u></strong></p> | ||
+ | <p><em>Present: Mark, Micah, Maddie, Collin, Alex</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Measured the OD730 of two cyanobacteria cultures <em>[Maddie]</em></li> | ||
+ | <ul> | ||
+ | <li>6.254 (#1), 4.047 (#4)</li> | ||
+ | </ul> | ||
+ | <li>Performed gel electrophoresis on lac+RBS+uvsE+RBS+blue chromoprotein+pSB1C3, lac+RBS+Dsup+pSB4C5, Trc+Dsup, PCP+Dsup, Trc+uvsE, and PCP+uvsE <em>[Mark, Collin]</em> | ||
+ | <li>Performed PCR to add golden gate ends onto phrAT <em>[Alex]</em></li> | ||
+ | <li>Prepared overnight cultures of lac+RBS+pSB1C3, lac+RBS+uvsE+RBS+blue chromoprotein+pSB1A2 (3 cultures; #1, #2, #3), and lac+RBS+Dsup+RBS+blue chromoprotein+pSB1C3 <em>[Alex]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 11--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Friday, August 11</u></strong></p> | ||
+ | <p><em>Present: Micah, Maddie</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Miniprepped the 3 cultures with lac+RBS+uvsE+RBS+blue chromoprotein+pSB1A2 <em>[Micah]</em></li> | ||
+ | <li>Digested lac+RBS+pSB1C3, uvsE+pSB1C3, phrAT+pSB1C3, phrAC+pSB1C3, Trc+Dsup, and PCP+uvsE <em>[Maddie]</em></li> | ||
+ | <li>Performed gel electrophoresis on the digested lac+RBS+pSB1C3, uvsE+pSB1C3, phrAT+pSB1C3, phrAC+pSB1C3, Trc+Dsup, and PCP+uvsE <em>[Maddie]</em></li> | ||
+ | <li>Tranformed plac Or2-62 (BBa_I12210) into DH5α E. Coli cells <em>[Micah]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 12--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Saturday, August 12</u></strong></p> | ||
+ | <p><em>Present: Mark, Micah, Collin, Alex</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
Line 954: | Line 2,060: | ||
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li>Met up with the Missouri S&T iGEM Team in Rolla Missouri <em>[Alex, Collin, Mark, Micah]</em></li> | ||
</div> | </div> | ||
</div> | </div> | ||
− | <!-- | + | <!--August 13--> |
<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Sunday, August 13</u></strong></p> | ||
+ | <p><em>Present: Maddie, Alex</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Gel purified lac+RBS+pSB1C3,lac+RBS+phrAC+pSB1C3, and lac+RBS+uvsE+pSB1C3 <em>[Maddie]</em></li> | ||
+ | <li>Ligated lac+RBS+uvsE and lac+RBS+phrAC <em>[Alex]</em></li> | ||
+ | <li>Transformed lac+RBS+uvsE and lac+RBS+phrAC into DH5α E. Coli cells <em>[Alex]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 14--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Monday, August 14</u></strong></p> | ||
+ | <p><em>Present: Mark, Micah, Maddie, Alex</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Digested phrAT+pSB1C3 <em>[Maddie, Mark]</em></li> | ||
+ | <li>Prepared liquid cultures of lac+RBS+uvsE+pSB1C3 and lac+RBS+phrAC+pSB1C3 (3 cultures of each; #20, #21, #22) <em>[Alex, Mark]</em></li> | ||
+ | <li>Performed the UV irradiation test on DH5α cells with Dsup (with time intervals of 0, 1, 2, 3, and 4 hours) <em>[Micah, Mark, Maddie]</em></li> | ||
+ | <li>Performed PCR to add Golden Braid ends to Dsup <em>[Alex]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 15--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Tuesday, August 15</u></strong></p> | ||
+ | <p><em>Present: Mark, Micah, Maddie, Alex</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Counted the colonies on the plates from the previous day's UV irradiation test <em>[Micah, Mark, Alex]</em></li> | ||
+ | <li>Miniprepped the overnight cultures of lac+RBS+uvsE+pSB1C3 and lac+RBS+phrAC+pSB1C3 <em>[Maddie]</em></li> | ||
+ | <li>Digested phrAT+pSB1C3, lac+RBS+phrAC+pSB1C3, and lac+RBS+uvsE <em>[Mark]</em></li> | ||
+ | <li>Performed gel electrophoresis on the digested phrAT+pSB1C3, lac+RBS+phrAC+pSB1C3, and lac+RBS+uvsE <em>[Mark]</em></li> | ||
+ | <li>Prepared liquid cultures of induced lac+RBS+Dsup+RBS+Blue chromoprotein+pSB1C3, non-induced lac+RBS+Dsup+RBS+Blue chromoprotein+pSB1C3, control DH5α E. Coli cells and lac+RBS+uvsE <em>[Alex]</em></li> | ||
+ | <li>Performed PCR to add golden gate compatible ends to phrAT <em>[Maddie]</em></li> | ||
+ | <li>Prepared LB+CM mini plates <em>[Mark]</em></li> | ||
+ | |||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 16--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Wednesday, August 16</u></strong></p> | ||
+ | <p><em>Present: Zoe, Mark, Micah, Maddie, Alex</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Performed gel purification on phrAT+pSB1C3 <em>[Maddie]</em></li> | ||
+ | <li>Prepared 1200 mL of LB+Agar <em>[Maddie]</em></li> | ||
+ | <li>Performed the UV irradiation test on Dsup and the control <em>[Micah, Mark]</em></li> | ||
+ | <li>Performed restriction digestion on lac+RBS+uvsE+pSB1C3, lac+RBS+phrAC+pSB1C3, and lac+RBS+pSB1C3 <em>[Maddie, Mark]</em></li> | ||
+ | <li>Performed gel electrophoresis on the digested DNA <em>[Mark]</em></li> | ||
+ | <li>Ligated lac+RBS+uvSE+pSB1C3 with lac+RBS+blue chromoprotein, lac+RBS+pSB1C3 with blue chromoprotein, and lac+RBS+pSB1C3 with phrAT <em>[Zoe, Alex]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 17--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Thursday, August 17</u></strong></p> | ||
+ | <p><em>Present: Zoe, Mark, Micah, Maddie, Alex</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Digested lac+RBS+phrAC+pSB1C3 <em>[Maddie]</em></li> | ||
+ | <li>Counted the colonies on the plates from the previous day's UV irradiation test <em>[Micah, Mark, Alex, Zoe]</em></li> | ||
+ | <li>Performed a PCR gradient of uvsE for GoldenBraid <em>[Maddie]</em></li> | ||
+ | <li>Prepared overnight cultures of Dsup+BCP, lac+RBS+phrAT+pSB1C3, and RBS+BCP+pSB1C3 <em>[Mark]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 18--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Friday, August 18</u></strong></p> | ||
+ | <p><em>Present: Zoe, Mark, Micah, Maddie, Alex</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Prepared glycerol stocks of DH5α E. coli cells that contained lac+RBS+phrAT+pSB1C3, lac+RBS+BCP+pSB1C3, and lac+RBS+Dsup+RBS+BCP+pSB1C3 <em>[Mark]</em></li> | ||
+ | <li>Miniprepped lac+RBS+phrAT+pSB1C3 <em>[Zoe]</em></li> | ||
+ | <li>Performed a UV irradiation test for Dsup and the Blue chromoprotein control <em>[Micah, Mark]</em></li> | ||
+ | <li>Performed PCR for uvsE <em>[Maddie, Zoe]</em></li> | ||
+ | <li>Digested lac+RBS+phrAT+pSB1C3 <em>[Alex]</em></li> | ||
+ | <li>Ran a gel electrophoresis for the digested lac+RBS+phrAT+pSB1C3 <em>[Mark]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 19--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Saturday, August 19</u></strong></p> | ||
+ | <p><em>Present: Zoe, Maddie, Mark</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Counted colonies on the plates from the UV irradiation test on Dsup <em>[Zoe, Maddie, Mark]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 20--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Sunday, August 20</u></strong></p> | ||
+ | <p><em>Present: Micah</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Prepared overnight cultures of DH5α E. coli cells with lac+RBS+blue chromoprotein+pSB1C3, lac+RBS+Dsup+RBS+blue chromoprotein+pSB1C3, and lac+RBS+uvsE+RBS+blue chromoprotein+pSB1C3 (#1) <em>[Micah]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 21--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Monday, August 21</u></strong></p> | ||
+ | <p><em>Present:Mark, Micah</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Prepared overnight cultures of DH5α E. coli cells with pSB1C3+lac+RBS+phrAC+RBS+blue chromoprotein (2 cultures, #1 and #2), pSB1C3+lac+RBS+blue chromoprotein, and pSB1C3+lac+RBS+Dsup+RBS+blue chromoprotein <em>[Mark]</em></li> | ||
+ | <li>Miniprepped DH5α E. coli cell cultures with lac+RBS+blue chromoprotein+pSB1C3, lac+RBS+Dsup+RBS+blue chromoprotein+pSB1C3, and lac+RBS+uvsE+RBS+blue chromoprotein+pSB1C3 (#1) <em>[Micah]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | <ol> | ||
+ | <li>Watched the Great American Eclipse of 2017 <em>[Micah, Mark, Zoe, Maddie, Alex, Collin]</em></li> | ||
+ | </ol> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 22--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Tuesday, August 22</u></strong></p> | ||
+ | <p><em>Present: Mark, Zoe, Micah, Alex, Maddie</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Miniprepped lac+RBS+phrAC+RBS+BCP+pSB1C3 <em>[Zoe]</em></li> | ||
+ | <li>Gel purified lac+RBS+phrAT+pSB1C3 <em>[Maddie]</em></li> | ||
+ | <li>Prepared LB+CM plates <em>[Mark]</em></li> | ||
+ | <li>Performed the UV irradiation test on uvsE, phrAC, and the blue chromoprotein control <em>[Micah, Mark]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 23--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Wednesday, August 23</u></strong></p> | ||
+ | <p><em>Present: Zoe, Maddie, Mark, Alex, Micah</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Counted the colonies on the plates from the previous day's UV irradiation test <em>[Micah, Alex, Mark, Zoe, Maddie]</em></li> | ||
+ | <li>Streaked lac+RBS+uvsE+RBS+BCP+pSB1C3 and lac+RBS+phrAC+RBS+BCP+pSB1C3 onto LB+CM plates <em>[Micah]</em></li> | ||
+ | <li>Ligated lac+RBS+phrAT+pSB1C3 with RBS+BCP <em>[Micah]</em></li> | ||
+ | <li>Transformed DH5α E. coli cells with lac+RBS+phrAT+RBS+BCP+pSB1C3 <em>[Mark]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 24--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Thursday, August 24</u></strong></p> | ||
+ | <p><em>Present:Micah</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Prepared overnight cultures of DH5α E. coli cells with lac+RBS+phrAT+RBS+BCP+pSB1C3 <em>{Micah]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 25--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Friday, August 25</u></strong></p> | ||
+ | <p><em>Present: Zoe</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Miniprepped lac+RBS+phrAT+RBS+BCP+pSB1C3 <em>[Zoe]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 26--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Saturday, August 26</u></strong></p> | ||
<p><em></em></p> | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 27--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Sunday, August 27</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 28--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Monday, August 28</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 29--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Tuesday, August 29</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 30--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Wednesday, August 30</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--August 31--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Thursday, August 31</u></strong></p> | ||
+ | <p><em>Present: Collin</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li>Prepared overnight cultures from glycerol stock of lac+RBS+BCP+pSB1C3 <em>[Collin]</em></li> | |
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
Line 969: | Line 2,327: | ||
</div> | </div> | ||
− | <!-- | + | <!--September 1--> |
<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Friday, September 1</u></strong></p> | ||
<p><em></em></p> | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--September 2--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Saturday, September 2</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--September 3--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Sunday, September 3</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--September 4--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Monday, September 4</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--September 5--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Tuesday, September 5</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--September 6--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Wednesday, September 6</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--September 7--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Thursday, September 7</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--September 8--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Friday, September 8</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--September 9--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Saturday, September 9</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--September 10--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Sunday, September 10</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--September 11--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Monday, September 11</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--September 12--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Tuesday, September 12</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--September 13--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Wednesday, September 13</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--September 14--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Thursday, September 14</u></strong></p> | ||
+ | <p><em>Present:Mark</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li>Transformed Lac+RBS+BCP+pSB1C3 into E.coli cells <em>[Mark]</em></li> | |
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
Line 981: | Line 2,457: | ||
</div> | </div> | ||
− | <!-- | + | <!--September 15--> |
<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Friday, September 15</u></strong></p> | ||
<p><em></em></p> | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--September 16--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Saturday, September 16</u></strong></p> | ||
+ | <p><em>Present:Maddie</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
− | + | <li>Started a cyanobacteria culture <em>[Maddie]</em></li> | |
+ | <li>Performed GoldenBraid PCR for uvsE, phrAC, phrAT, and Dsup <em>[Maddie]</em></li> | ||
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
</div> | </div> | ||
</div> | </div> | ||
+ | |||
+ | <!--September 17--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Sunday, September 17</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--September 18--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Monday, September 18</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--September 19--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Tuesday, September 19</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--September 20--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Wednesday, September 20</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--September 21--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Thursday, September 21</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--September 22--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Friday, September 22</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--September 23--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Saturday, September 23</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--September 24--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Sunday, September 24</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--September 25--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Monday, September 25</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--September 26--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Tuesday, September 26</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--September 27--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Wednesday, September 27</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--September 28--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Thursday, September 28</u></strong></p> | ||
+ | <p><em>Present: Collin</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Refreshed Cyanobacteria culture <em>[Collin]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--September 29--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Friday, September 29</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--September 30--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Saturday, September 30</u></strong></p> | ||
+ | <p><em>Present:Collin, Micah, Mark</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Performed PCR on Dsup, uvsE, phrAC, and phrAT for the GoldenBraid assembly <em>[Collin]</em></li> | ||
+ | <li>Performed the UV irradiaton test for uvsE, phrAC, and the control <em>[Micah, Mark, Collin]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 1--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Sunday, October 1</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 2--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Monday, October 2</u></strong></p> | ||
+ | <p><em>Present: Collin</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Measured the ODs of the Cyanobacteria culture <em>[Collin]</em></li> | ||
+ | <ul> | ||
+ | <li> 3.413 (#1), 0.1370 (#2)</li> | ||
+ | </ul> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 3--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Tuesday, October 3</u></strong></p> | ||
+ | <p><em>Present:Maddie</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li> Measured the OD of the cyanobacteria culture <em>[Maddie]</em></li> | ||
+ | <ul> | ||
+ | <li> 0.1452 (#2)</li> | ||
+ | </ul> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 4--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Wednesday, October 4</u></strong></p> | ||
+ | <p><em>Present:Maddie</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li> Measured the OD of the cyanobacteria culture <em>[Maddie]</em></li> | ||
+ | <ul> | ||
+ | <li> 0.3725 (#2)</li> | ||
+ | </ul> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 5--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Thursday, October 5</u></strong></p> | ||
+ | <p><em>Present:Maddie</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li> Measured the OD of the cyanobacteria culture <em>[Maddie]</em></li> | ||
+ | <ul> | ||
+ | <li> 0.6746 (#2)</li> | ||
+ | </ul> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 6--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Friday, October 6</u></strong></p> | ||
+ | <p><em>Present:Mark</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Prepared LB Media <em>[Mark]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 7--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Saturday, October 7</u></strong></p> | ||
+ | <p><em>Present:Mark, Maddie</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Prepared LB Agar <em>[Mark]</em></li> | ||
+ | <li>Prepared overnight cultures of BCP, uvsE, and phrAC <em>[Maddie]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 8--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Sunday, October 8</u></strong></p> | ||
+ | <p><em>Present:Mark, Micah</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Prepared for a UV irradiation test for uvsE, phrAC, and BCP control <em>[Mark, Micah]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 9--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Monday, October 9</u></strong></p> | ||
+ | <p><em>Present: Collin</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li> Measured the OD of the cyanobacteria culture <em>[Maddie]</em></li> | ||
+ | <ul> | ||
+ | <li> 3.1760 (#2)</li> | ||
+ | </ul> | ||
+ | </ol> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 10--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Tuesday, October 10</u></strong></p> | ||
+ | <p><em>Present: Collin</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Digested neomycin phosphotransferase (nptII) and pSB1C3 <em>[Colin]</em></li> | ||
+ | <li>Ran a gel electrophoresis for the digested nptII and pSB1C3 <em>[Collin]</em></li> | ||
+ | <li>Transformed uvsE+BCP into E. coli <em>[Collin]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 11--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Wednesday, October 11</u></strong></p> | ||
+ | <p><em>Present: Collin</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Made Kanamycin plates <em>[Collin]</em></li> | ||
+ | <li>Prepared bacterial stabs of Dsup, phrAC, and phrAT for the UChicago team <em>[Collin]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 12--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Thursday, October 12</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 13--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Friday, October 13</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 14--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Saturday, October 14</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 15--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Sunday, October 15</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 16--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Monday, October 16</u></strong></p> | ||
+ | <p><em>Present: Mark</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Gel purified pSB1C3 and nptII <em>[Mark]</em></li> | ||
+ | <li>Ligated pSB1C3 and nptII <em>[Mark]</em></li> | ||
+ | <li>Transformed pSB1C3+nptII <em>[Mark]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 17--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Tuesday, October 17</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 18--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Wednesday, October 18</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 19--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Thursday, October 19</u></strong></p> | ||
+ | <p><em>Present: Collin</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Streaked phrAT, phrAC, BCP, uvsE, and BCP <em>[Collin]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 20--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Friday, October 20</u></strong></p> | ||
+ | <p><em>Present: Collin</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Transformed lac+RBS+Dsup+BCP into E.coli cells <em>[Collin]</em></li> | ||
+ | <li>Prepared cultures of lac+RBS+Dsup, lac+RBS+BCP, and lac+RBS+uvsE <em>[Collin]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 21--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Saturday, October 21</u></strong></p> | ||
+ | <p><em>Present: Micah</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Transformed medium promoter+medium RBS (BBa_K608006) into E. coli <em>[Micah]</em></li> | ||
+ | <li>Prepared cultures from previous transformation <em>[Micah]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 22--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Sunday, October 22</u></strong></p> | ||
+ | <p><em>Present:Collin</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Made glycerol stocks of lac+RBS+Dsup+RBS+BCP+pSB1C3 <em>[Collin]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 23--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Monday, October 23</u></strong></p> | ||
+ | <p><em>Present: Collin</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Miniprepped lac+RBS+BCP+pSB1C3 <em>[Collin]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 24--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Tuesday, October 24</u></strong></p> | ||
+ | <p><em>Present: Collin, Mark</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Prepared two overnights of Dsup and one of BCP <em>[Collin]</em></li> | ||
+ | <li>Digested nptII and med promoter + med RBS + pSB1C3 <em>[Mark]</em></li> | ||
+ | <li>Ran gel electrophoresis on the digested DNA <em>[Mark]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 25--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Wednesday, October 25</u></strong></p> | ||
+ | <p><em>Present:Mark, Collin, Micah, Maddie, Zoe</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Performed the UV irradiation test on Dsup and the control BCP <em>[Micah, Collin, Maddie, Zoe]</em></li> | ||
+ | <li>Gel purified nptII and med promoter + med RBS + pSB1C3 <em>[Mark]</em></li> | ||
+ | <li>Ligated nptII to the med promoter + med RBS + pSB1C3 <em>[Mark, Collin]</em></li> | ||
+ | <li>Transformed the ligated DNA into E. coli cells <em>[Collin, Mark]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 26--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Thursday, October 26</u></strong></p> | ||
+ | <p><em>Present: Micah</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Prepared an overnight culture of med promoter + med RBS + nptII <em>[Micah]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 27--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Friday, October 27</u></strong></p> | ||
+ | <p><em>Present: Micah, Alex</em></p> | ||
+ | <p><strong> Lab Work </strong></p> | ||
+ | <ol> | ||
+ | <li>Miniprepped the med promoter + med RBS + nptII <em>[Micah]</em></li> | ||
+ | <li>Prepared the DNA to be sent to the registry <em>[Alex, Micah]</em></li> | ||
+ | </ol> | ||
+ | <p><strong> Outside Work / Discussion </strong><p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 28--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Saturday, October 28</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 29--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Sunday, October 29</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 30--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Monday, October 30</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--October 31--> | ||
+ | <div class="mySlides fade"> | ||
+ | <div style="height: 200px; width: 100%;"> | ||
+ | <p><strong><u>Tuesday, October 31</u></strong></p> | ||
+ | <p><em></em></p> | ||
+ | <p>No work done today!</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
<script> | <script> |
Latest revision as of 03:17, 2 November 2017
Notebook
Click on a date to see what we did on that day!
June 2017 | ||||||
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Sunday | Monday | Tuesday | Wednesday | Thursday | Friday | Saturday |
1 | 2 | 3 | ||||
4 | 5 | 6 | 7 | 8 | 9 | 10 |
11 | 12 | 13 | 14 | 15 | 16 | 17 |
18 | 19 | 20 | 21 | 22 | 23 | 24 |
25 | 26 | 27 | 28 | 29 | 30 | |