Difference between revisions of "Team:UIUC Illinois/Parts"

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<a class="pull-left" href="https://2017.igem.org/Team:UIUC_Illinois"><img id="navImage" src="https://static.igem.org/mediawiki/2017/2/2d/UIUC2017logo.png"></a>
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<div class="topnav collapse navbar-collapse" id="bs-example-navbar-collapse-1">
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<a id="centered" href="https://2017.igem.org/Team:UIUC_Illinois">Home</a>
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<a id="centered" href="https://2017.igem.org/Team:UIUC_Illinois/Project">Project</a>
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<a id="centered" href="https://2017.igem.org/Team:UIUC_Illinois/Team">Team</a>
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<a id="centered" href="https://2017.igem.org/Team:UIUC_Illinois/Parts">Parts</a>
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                                <a id="centered" href="https://2017.igem.org/Team:UIUC_Illinois/Wet_lab">Wet lab</a>                               
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                                                href="https://2017.igem.org/Team:UIUC_Illinois/Collaboration">Collaboration</a>
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<h1>Parts</h1>
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<h2 id="topHeader">Parts</h2>
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        <p>We used two parts for our experiments. Both constructs were assembled using NEB Gibson Assembly Master Mix E2611.</p>
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        <p>The part shown in the left panel consists of the Pyrococcus furiosus DNA polymerase insert gene in the JOE vector.  The DNA polymerase is codon-optimized. This part expresses the P. furiosus DNA polymerase enzyme when transformed in DH5α. The P. furiosus DNA polymerase enzyme is fused to a single- stranded binding protein. This gives the enzyme characteristics similar to the commercial Phusion DNA polymerase. The sequence for this part could not be verified and was not sent to iGEM HQ.</p>
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        <p>The part shown in the right panel consists of the Thermotoga maritima DNA ligase insert gene in the JOE vector. This part expresses the T. maritima DNA ligase enzyme when transformed in DH5α. The sequence for this part was successfully verified and was sent to iGEM HQ. </p>
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        The registry link is as follows:<a href="http://parts.igem.org/Part:BBa_K2468000"> http://parts.igem.org/Part:BBa_K2468000</a>
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        <p>Both parts have indicated potential for behaving as expected, however, we are conducting more experiments to obtain concrete results regarding the behavior of each plasmid construct. One experiment has indicated the successful behavior of the P. furiosus DNA polymerase enzyme.</p>
  
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<h5>Note</h5>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<p><span>Made by UIUC_Illinois iGEM</span></p>
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<h5>Adding parts to the registry</h5>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<h5>What information do I need to start putting my parts on the Registry?</h5>
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<p>The information needed to initially create a part on the Registry is:</p>
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<li>Part Name</li>
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<li>Part type</li>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<h5>Inspiration</h5>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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<h5>Part Table </h5>
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
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<groupparts>iGEM17 UIUC_Illinois</groupparts>
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Latest revision as of 03:26, 2 November 2017

We used two parts for our experiments. Both constructs were assembled using NEB Gibson Assembly Master Mix E2611.

The part shown in the left panel consists of the Pyrococcus furiosus DNA polymerase insert gene in the JOE vector. The DNA polymerase is codon-optimized. This part expresses the P. furiosus DNA polymerase enzyme when transformed in DH5α. The P. furiosus DNA polymerase enzyme is fused to a single- stranded binding protein. This gives the enzyme characteristics similar to the commercial Phusion DNA polymerase. The sequence for this part could not be verified and was not sent to iGEM HQ.

The part shown in the right panel consists of the Thermotoga maritima DNA ligase insert gene in the JOE vector. This part expresses the T. maritima DNA ligase enzyme when transformed in DH5α. The sequence for this part was successfully verified and was sent to iGEM HQ.

The registry link is as follows: http://parts.igem.org/Part:BBa_K2468000

Both parts have indicated potential for behaving as expected, however, we are conducting more experiments to obtain concrete results regarding the behavior of each plasmid construct. One experiment has indicated the successful behavior of the P. furiosus DNA polymerase enzyme.

Made by UIUC_Illinois iGEM