Difference between revisions of "Team:UIUC Illinois/Collaboration"
| (37 intermediate revisions by the same user not shown) | |||
| Line 58: | Line 58: | ||
<body> | <body> | ||
| + | |||
| + | <!-- sidebar --> | ||
| + | <nav id="sidenavbar"> | ||
| + | <ul style="list-style: none;"> | ||
| + | <li> | ||
| + | <a href="#COLLABORATION" data-number="1"> | ||
| + | <span class="cd-dot"></span> | ||
| + | <span class="cd-label">COLLABORATION</span> | ||
| + | </a> | ||
| + | </li> | ||
| + | <li> | ||
| + | <a href="#SPONSORS" data-number="2"> | ||
| + | |||
| + | <span class="cd-dot"></span> | ||
| + | <span class="cd-label">SPONSORS</span> | ||
| + | </a> | ||
| + | </li> | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | </ul> | ||
| + | </nav> | ||
| + | |||
| + | |||
<aside id="fh5co-hero"> | <aside id="fh5co-hero"> | ||
<div class="fh5co-cta" style="background-image: url(https://static.igem.org/mediawiki/2017/d/d9/UIUC-collaboration.jpeg);"> | <div class="fh5co-cta" style="background-image: url(https://static.igem.org/mediawiki/2017/d/d9/UIUC-collaboration.jpeg);"> | ||
| Line 68: | Line 94: | ||
</div> | </div> | ||
</aside> | </aside> | ||
| − | + | ||
<div id="fh5co-work-section" class="fh5co-light-grey-section"> | <div id="fh5co-work-section" class="fh5co-light-grey-section"> | ||
<div class="row"> | <div class="row"> | ||
| Line 76: | Line 102: | ||
<div class="text-center" style="padding-left: 2em; height: 200px; color:black"> | <div class="text-center" style="padding-left: 2em; height: 200px; color:black"> | ||
<div id="start" class="col-md-10 col-md-offset-1 fh5co-heading animate-box"> | <div id="start" class="col-md-10 col-md-offset-1 fh5co-heading animate-box"> | ||
| − | <h1 id="homeH">Collaboration</h1> | + | <h1 id="homeH" >Collaboration</h1> |
| − | <br> | + | <br id="COLLABORATION"> |
<p id="homeP"> | <p id="homeP"> | ||
We reached out to Team Northwestern to establish a collaboration that would allow us to test our homemade Gibson Assembly recipe on Northwestern’s construct. We received the backbone BBa_J32015, the PelB insert, and the associated PCR primers to test out our method and assemble the PelB-saCas9-His construct. The expected workflow involved designing Gibson assembly primers specific to the vector and insert, assembling the construct, transforming the assembled construct into DH5α cells, and verifying the assembly success via colony PCR and sequencing. </p> | We reached out to Team Northwestern to establish a collaboration that would allow us to test our homemade Gibson Assembly recipe on Northwestern’s construct. We received the backbone BBa_J32015, the PelB insert, and the associated PCR primers to test out our method and assemble the PelB-saCas9-His construct. The expected workflow involved designing Gibson assembly primers specific to the vector and insert, assembling the construct, transforming the assembled construct into DH5α cells, and verifying the assembly success via colony PCR and sequencing. </p> | ||
| Line 97: | Line 123: | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| Line 149: | Line 129: | ||
<div class="text-center" style="padding-left: 2em; height: 200px; color:black"> | <div class="text-center" style="padding-left: 2em; height: 200px; color:black"> | ||
<div id="start" class="col-md-10 col-md-offset-1 fh5co-heading animate-box"> | <div id="start" class="col-md-10 col-md-offset-1 fh5co-heading animate-box"> | ||
| − | <h1 id="homeH">Sponsors</h1> | + | <h1 id="homeH">Sponsors</h1><br id="SPONSORS"> |
</div> | </div> | ||
| Line 158: | Line 138: | ||
</div> | </div> | ||
| − | <div class="col-md-3"> | + | <div class="col-md-3" > |
| − | <a href="http://illinois.edu/"><img src="https://static.igem.org/mediawiki/ | + | <a href="http://illinois.edu/"><img src="https://static.igem.org/mediawiki/2017/4/43/UIUC-logo%2A.svg" alt="UIUC" style="width:150px;height:150px;" align="right"/></a> |
</div> | </div> | ||
| − | <div class="col-md-3"> | + | <div class="col-md-3" style="left:5px;"> |
<a href="http://www.igb.illinois.edu/"><br> | <a href="http://www.igb.illinois.edu/"><br> | ||
| − | <img src="https://static.igem.org/mediawiki/ | + | <img src="https://static.igem.org/mediawiki/2017/6/68/UIUC-igb.jpeg" alt="IGB" style="width:270px;height:100px;"/></a> |
</div> | </div> | ||
<div class="col-md-3"> | <div class="col-md-3"> | ||
| − | <a href="https:// | + | <a href="https://2017.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2017/5/54/UIUC-igem.png" alt="iGEM" style="width:170px;height:170px;" align="left"/></a> |
</div> | </div> | ||
</div> | </div> | ||
| Line 173: | Line 153: | ||
<div class="row"> | <div class="row"> | ||
| − | <div class="col-md-2" style="left: | + | <div class="col-md-2" style="left:960px;top:-37px;"> |
| − | <a href="https:// | + | <a href="https://www.neb.com/"><img src="https://static.igem.org/mediawiki/2017/1/11/UIUC-bio.png" alt="New England Biolabs" style="height:140px" align="right"/> </a> |
</div> | </div> | ||
<br> | <br> | ||
| − | <div class="col-md-3" style="position:absolute; left: | + | <div class="col-md-3" style="position:absolute; left:180px;"> |
| − | <a href="http://www. | + | <a href="http://www.agilent.com/home"><img src="https://static.igem.org/mediawiki/2017/7/7c/UIUC-agilent.jpeg" style="height:140px;" align="center"/> </a> |
</div> | </div> | ||
| − | <div class="col-md-3" style="position:absolute; left:220px;top: | + | <div class="col-md-3" style="position:absolute; left:220px;top:2400px;"> |
| − | <a href="http://www.idtdna.com/site"><img src="https://static.igem.org/mediawiki/ | + | <a href="http://www.idtdna.com/site"><img src="https://static.igem.org/mediawiki/2017/c/cd/UIUC-idna.png" alt="IDT" style="height:80px;" align="right"/> </a> |
</div> | </div> | ||
</div> | </div> | ||
Latest revision as of 03:44, 2 November 2017
Collaboration
We reached out to Team Northwestern to establish a collaboration that would allow us to test our homemade Gibson Assembly recipe on Northwestern’s construct. We received the backbone BBa_J32015, the PelB insert, and the associated PCR primers to test out our method and assemble the PelB-saCas9-His construct. The expected workflow involved designing Gibson assembly primers specific to the vector and insert, assembling the construct, transforming the assembled construct into DH5α cells, and verifying the assembly success via colony PCR and sequencing.
Once testing proved successful, Northwestern would have received the DNA polymerase and DNA ligase lysate to assemble their PelB-saCas9-His construct using optimal ratios that we established. The success of the assembly would be verified via colony PCR and sequencing.
Unfortunately, we faced unforeseen delays in the verification of our construct and in successful assembly of our test devices. Although we were in communication throughout the project, we were not able to pursue this plan completely.
Sponsors
