Difference between revisions of "Team:Gaston Day School/Part"
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<h3 class="subtitle">Baseline Corrected of Control vs. GlmY</h3> | <h3 class="subtitle">Baseline Corrected of Control vs. GlmY</h3> | ||
<img class="pic" src="https://static.igem.org/mediawiki/2017/2/2c/Baseline-corrected_of_Control_vs._GlmY.jpg"> | <img class="pic" src="https://static.igem.org/mediawiki/2017/2/2c/Baseline-corrected_of_Control_vs._GlmY.jpg"> | ||
| + | <p style="text-align:center;">Figure 1 shows that the plasmid carrying GlmY gives a small but significant increase in the resistance to low concentrations of isobutanol even though no promoter is present. At 60 ul isobutanol, the control had 12% survival while the culture with the GlmY plasmid had 25% survival. This increased resistance to isobutanol did not persist at higher concentrations.</p> | ||
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| − | <h3 class="subtitle"> | + | <h3 class="subtitle">Construct 1 (GlmY with a constitutive promoter):<h3> |
| − | < | + | <h4 style="margin-left: 120px;">Increase regulation of GlmZ which activates glmS expression.</h4> |
| − | + | <img class="pic" style="height:240px ;width:480px;" src="https://static.igem.org/mediawiki/2017/7/7d/%E5%BE%AE%E4%BF%A1%E5%9B%BE%E7%89%87_20171101234321.jpg"> | |
| − | + | <p style="text-align: center;">Figure 2 shows a characterized construct of part number BBa_K1320100. J23102 is a constitutive promoter, while K2221000 is GlmY.</p> | |
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Latest revision as of 03:52, 2 November 2017
Part
Baseline Corrected of Control vs. GlmY
Figure 1 shows that the plasmid carrying GlmY gives a small but significant increase in the resistance to low concentrations of isobutanol even though no promoter is present. At 60 ul isobutanol, the control had 12% survival while the culture with the GlmY plasmid had 25% survival. This increased resistance to isobutanol did not persist at higher concentrations.
Construct 1 (GlmY with a constitutive promoter):
Increase regulation of GlmZ which activates glmS expression.
Increase regulation of GlmZ which activates glmS expression.
Figure 2 shows a characterized construct of part number BBa_K1320100. J23102 is a constitutive promoter, while K2221000 is GlmY.
