Difference between revisions of "Team:SCU China"

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                                     <p class="scu_title">Rhythmic Production of Melatonin in <i>E.coli</i></p>
 
                                     <p class="scu_title">Rhythmic Production of Melatonin in <i>E.coli</i></p>
 
                                 </div>
 
                                 </div>
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                                 <p class="scu_vice_title">Nowadays, insomnia and circadian rhythm disorders are increasingly plaguing individuals' daily life.
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                                 <div class="scu_vice_title">SCU_China</div>
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                                    According to NIH reports, around 50-70 million U.S. adults are suffering from sleep insufficiency and poor-quality sleep.
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                                    The circadian rhythm in human is predominantly under the regulation of melatonin, a hormone produced by pineal gland
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                                    periodically. This year we want to use engineered <i>E.coli</i> to mimic this process in human. We propose to <strong>construct a melatonin
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                                        biosynthesis pathway in <i>E.coli</i> to produce melatonin</strong>, while simultaneously<strong> couple this process with periodically-optimized
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                                        repressilator</strong>, a synthetic genetic oscillator with higher precision, with the intention to <strong>render melatonin production
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                                        resemble mammalian periodicity in<i> E.coli</i>.</strong>  </p>
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                                 <div class="scu_down" id="scu_down">
 
                                 <div class="scu_down" id="scu_down">
 
                                     <p class="scu_down">learn more about us</p>
 
                                     <p class="scu_down">learn more about us</p>
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                                 <a href="https://2017.igem.org/Team:SCU_China">Home</a>
 
                                 <a href="https://2017.igem.org/Team:SCU_China">Home</a>
 
                             </li>
 
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                                 </a>
 
                                 </a>
 
                                 <ul class="dropdown-menu">
 
                                 <ul class="dropdown-menu">
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                                    <li><a href="https://2017.igem.org/Team:SCU_China/Malatonion">Melatonin</a>
 
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                                    </li>
 
 
                                     <li><a href="https://2017.igem.org/Team:SCU_China/Repressilator">Repressilator</a>
 
                                     <li><a href="https://2017.igem.org/Team:SCU_China/Repressilator">Repressilator</a>
 
                                     </li>
 
                                     </li>
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                                    <li><a href="https://2017.igem.org/Team:SCU_China/Malatonion">Melatonin</a>
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                                    </li>
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                                     <li><a href="https://2017.igem.org/Team:SCU_China/InterLab">InterLab</a></li>
 
                                     <li><a href="https://2017.igem.org/Team:SCU_China/InterLab">InterLab</a></li>
 
                                     <li><a href="https://2017.igem.org/Team:SCU_China/Parts">Parts</a></li>
 
                                     <li><a href="https://2017.igem.org/Team:SCU_China/Parts">Parts</a></li>
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                             </li>
 
                             </li>
 
                             <li class="dropdown lee_nav_modeling">
 
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                                 <a href="https://2017.igem.org/Team:SCU_China/Model" class="dropdown-toggle" data-toggle="dropdown">
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                                 <a href="https://2017.igem.org/Team:SCU_China/Model" class="dropdown-toggle"
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                                  data-toggle="dropdown">
 
                                     Modeling
 
                                     Modeling
 
                                 </a>
 
                                 </a>
 
                                 <ul class="dropdown-menu">
 
                                 <ul class="dropdown-menu">
 
                                     <li><a href="https://2017.igem.org/Team:SCU_China/Model">Overview</a>
 
                                     <li><a href="https://2017.igem.org/Team:SCU_China/Model">Overview</a>
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                                    </li>
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                                    <li>
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                                        <a href="https://2017.igem.org/Team:SCU_China/Model/Repressilator">Repressilator</a>
 
                                     </li>
 
                                     </li>
 
                                     <li><a href="https://2017.igem.org/Team:SCU_China/Model/Melatonin">Melatonin</a>
 
                                     <li><a href="https://2017.igem.org/Team:SCU_China/Model/Melatonin">Melatonin</a>
 
                                     </li>
 
                                     </li>
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                                    <li><a href="https://2017.igem.org/Team:SCU_China/Model/Repressilator">Repressilator</a>
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                                    </li>
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                                     <li><a href="https://2017.igem.org/Team:SCU_China/Model/RBSModification">RBS
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                                     <li><a href="https://2017.igem.org/Team:SCU_China/Model/RBSModification">RBS Modification</a>
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                                        Modification</a>
  
 
                                     </li>
 
                                     </li>
 
                                 </ul>
 
                                 </ul>
 
                             </li>
 
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                             <li class="dropdown lee_nav_modeling">
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                             <li class="dropdown lee_nav_human">
 
                                 <a href="https://2017.igem.org/Team:SCU_China/HP/Silver" class="dropdown-toggle"
 
                                 <a href="https://2017.igem.org/Team:SCU_China/HP/Silver" class="dropdown-toggle"
 
                                   data-toggle="dropdown">Human Practices
 
                                   data-toggle="dropdown">Human Practices
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                                     <li><a href="https://2017.igem.org/Team:SCU_China/HP/Silver/Meetup">Meet-up</a>
 
                                     <li><a href="https://2017.igem.org/Team:SCU_China/HP/Silver/Meetup">Meet-up</a>
 
                                     </li>
 
                                     </li>
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                                     <li><a href="https://2017.igem.org/Team:SCU_China/HP/Silver/Engagement">Engagement</a>
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                                     <li>
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                                        <a href="https://2017.igem.org/Team:SCU_China/HP/Silver/Engagement">Engagement</a>
 
                                     </li>
 
                                     </li>
 
                                     <li><a href="https://2017.igem.org/Team:SCU_China/Collaborations">Collaboration</a>
 
                                     <li><a href="https://2017.igem.org/Team:SCU_China/Collaborations">Collaboration</a>
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                                 <a href="https://2017.igem.org/Team:SCU_China/Attributions">
 
                                     Attribution
 
                                     Attribution
 
                                 </a>
 
                                 </a>
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                                    <li><a href="https://2017.igem.org/Team:SCU_China/Attributions#Team">Team</a></li>
 
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                                    <li><a href="https://2017.igem.org/Team:SCU_China/Attributions#Acknowledgement">Acknowledgement</a></li>
 
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                             </li>
 
                             </li>
 
                             <li class="dropdown lee_nav_gold">
 
                             <li class="dropdown lee_nav_gold">
  
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                                 <a href="https://2017.igem.org/Team:SCU_China/HP/Gold_Integrated" class="dropdown-toggle" data-toggle="dropdown">
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                                 <a href="https://2017.igem.org/Team:SCU_China/HP/Gold_Integrated" class="dropdown-toggle"
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                                  data-toggle="dropdown">
 
                                     Gold Medal
 
                                     Gold Medal
 
                                 </a>
 
                                 </a>
 
                                 <ul class="dropdown-menu">
 
                                 <ul class="dropdown-menu">
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                                     <li><a href="https://2017.igem.org/Team:SCU_China/HP/Gold_Integrated">Integrated HP</a></li>
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                                     <li><a href="https://2017.igem.org/Team:SCU_China/HP/Gold_Integrated">Integrated
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                                        HP</a></li>
 
                                     <li><a href="https://2017.igem.org/Team:SCU_China/HP/Improve">Improve</a></li>
 
                                     <li><a href="https://2017.igem.org/Team:SCU_China/HP/Improve">Improve</a></li>
 
                                     <li><a href="https://2017.igem.org/Team:SCU_China/Model">Model</a></li>
 
                                     <li><a href="https://2017.igem.org/Team:SCU_China/Model">Model</a></li>
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                             <li class="lee_nav_note">
 
                             <li class="lee_nav_note">
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                                 <a href="https://2017.igem.org/Team:SCU_China/Notebook" class="">
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                                     NoteBook
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                                     Notebook
 
                                 </a>
 
                                 </a>
 
                             </li>
 
                             </li>
 
                         </div>
 
                         </div>
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                        <div class="scu_main_page_wrapper">
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                            <div class="scu_main_page_title">
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                                <div class="scu_main_page_title2">Rhythmic Production of Melatonin in <i>E.coli</i>
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                                </div>
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                                <div class="overview-sub-title">SCU_China</div>
 +
                                <p class="scu_main_page_content_p2">
 +
                                    &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Nowadays, insomnia and circadian rhythm disorders are increasingly plaguing
 +
                                    individuals' daily life. The circadian rhythm in human is predominantly under the
 +
                                    regulation of melatonin, a hormone produced by pineal gland periodically in
 +
                                    accordance with day-and-night photoperiod. This year we wanted to use engineered
 +
                                    <i>E.coli</i> to mimic this process in human as a promising dosing machine. We proposed to
 +
                                    <strong>construct the melatonin biosynthesis pathway in <i>E.coli</i> to produce melatonin</strong>, and
 +
                                    simultaneously <strong>couple this process with optimized repressilator</strong>, a synthetic genetic
 +
                                    oscillator with higher precision and stability, to magnify the function of
 +
                                    repressilator and <strong>render melatonin production resemble mammalian periodicity in
 +
                                    <i>E.coli</i></strong>.
 +
                                </p>
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                            </div>
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                            <div class="scu_hp_image_single_main">
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                                <img src="https://static.igem.org/mediawiki/2017/7/7d/T-SCU_China-2017_Team_fig1.jpg">
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                            </div>
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                            <div class="col-lg-4 col-md-6 col-xs-12 scu_main_page_content">
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                                <div class="scu_main_page_gold">
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                                    <img src="https://static.igem.org/mediawiki/2017/3/3c/T-SCU_China-2017_%E9%87%91_copy.png">
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                                </div>
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                                <div class="scu_main_page_content_wrapper" id="left_wrapper">
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                                    <p class="scu_main_page_content_p" id="left">
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                                        1. Our human practices were integrated to the whole process, which made us
 +
                                        complete
 +
                                        the project better.<br>
 +
                                        2. We improved two previous part BBa_I13521 and BBa_E2050 and our BioBrick work
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                                        well
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                                        in lab condition.<br>
 +
                                        3. Our experimental design based on modelling results, such as the optimal
 +
                                        proportioning of synthetase.<br>
 +
                                        4. Engineered E.coli is cultured with artificial gastrointestinal juices and gut
 +
                                        flora to validate the substantial integration and survival condition based on
 +
                                        the
 +
                                        consideration for better safety and higher efficiency.
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                                    </p>
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                                </div>
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                            </div>
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                            <div class="col-lg-4 col-md-6 col-xs-12 scu_main_page_content">
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                                <div class="scu_main_page_gold">
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                                    <img src="https://static.igem.org/mediawiki/2017/7/7b/T-SCU_China-2017_silver_copy.png">
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                                <div class="scu_main_page_content_wrapper" id="middle_wrapper">
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                                    <p class="scu_main_page_content_p" id="middle">
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                                        1. There are 4 new BioBrick parts in our project as follow, BBa_K2276001,
 +
                                        BBa_K2276003, BBa_K2276004 and BBa_K2206005.<br>
 +
                                        2. We collaborated with SCU-WestChina and SiCAU-China in experiments and human
 +
                                        practice, received key assists from Peking University and offered wiki design
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                                        suggestions to team Purdue.<br>
 +
                                        3. We consulted experts of China Food and Drug Administration and West China
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                                        Sleeping Centre for both experimental designs and safety problems. Also, we
 +
                                        collected and analyzed convincing datas from our survey.
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                                    </p>
 +
                                </div>
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                            </div>
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Latest revision as of 03:44, 16 November 2017

Team:SCU China - 2017.igem.org

Rhythmic Production of Melatonin in E.coli

SCU_China

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Rhythmic Production of Melatonin in E.coli
SCU_China

      Nowadays, insomnia and circadian rhythm disorders are increasingly plaguing individuals' daily life. The circadian rhythm in human is predominantly under the regulation of melatonin, a hormone produced by pineal gland periodically in accordance with day-and-night photoperiod. This year we wanted to use engineered E.coli to mimic this process in human as a promising dosing machine. We proposed to construct the melatonin biosynthesis pathway in E.coli to produce melatonin, and simultaneously couple this process with optimized repressilator, a synthetic genetic oscillator with higher precision and stability, to magnify the function of repressilator and render melatonin production resemble mammalian periodicity in E.coli.

1. Our human practices were integrated to the whole process, which made us complete the project better.
2. We improved two previous part BBa_I13521 and BBa_E2050 and our BioBrick work well in lab condition.
3. Our experimental design based on modelling results, such as the optimal proportioning of synthetase.
4. Engineered E.coli is cultured with artificial gastrointestinal juices and gut flora to validate the substantial integration and survival condition based on the consideration for better safety and higher efficiency.

1. There are 4 new BioBrick parts in our project as follow, BBa_K2276001, BBa_K2276003, BBa_K2276004 and BBa_K2206005.
2. We collaborated with SCU-WestChina and SiCAU-China in experiments and human practice, received key assists from Peking University and offered wiki design suggestions to team Purdue.
3. We consulted experts of China Food and Drug Administration and West China Sleeping Centre for both experimental designs and safety problems. Also, we collected and analyzed convincing datas from our survey.

1. We constructed a team successfully, sharing not only academic knowledge but also life experiences with each other.
2.We received many kindly help from surroundings, including our college, instructor, experts in various fields, friendly fraternal teams and so on.We have clearly acknowledged their works.
3. We managed to meet all the Deliverables standards.
4. We participated in the Interlab Measurement Study.


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