Difference between revisions of "Team:FAFU-CHINA"
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<head> | <head> | ||
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<title>iGEM 2017_FAFU-CHINA</title> | <title>iGEM 2017_FAFU-CHINA</title> | ||
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| − | + | </head> | |
| − | <style | + | <body> |
| − | body { | + | <style> |
| − | background | + | body{ |
| − | + | margin: 0 auto; | |
| + | height:100%; | ||
| + | background:url(https://static.igem.org/mediawiki/2017/d/d0/T--FAFU-CHINA--12.1.png) scroll center; | ||
background-repeat:no-repeat; | background-repeat:no-repeat; | ||
| − | + | background-size:100%; | |
| − | + | } | |
| − | + | .maxx{ | |
| − | + | height:auto; | |
| − | + | width:60%; | |
| − | + | margin:0 auto; | |
| − | + | ||
} | } | ||
</style> | </style> | ||
| + | <div class="maxx"> | ||
| + | |||
| + | <style> | ||
| + | @charset "utf-8"; | ||
| + | body{ | ||
| + | margin: 0; | ||
| + | padding: 0; | ||
| + | -webkit-text-size-adjust: none; | ||
| + | /*background-image: url("../img/hat.jpg");*/ | ||
| + | font: 12px "宋体"; | ||
| + | } | ||
| + | |||
| + | |||
| + | ul, ol, li { | ||
| + | list-style-type: none; | ||
| + | vertical-align: 0 | ||
| + | } | ||
| + | |||
| + | |||
| + | .nav { | ||
| + | width: 100%; | ||
| + | margin: 0 auto; | ||
| + | text-align: center; | ||
| + | } | ||
| + | |||
| + | .menu, .menu ul { | ||
| + | list-style: none; | ||
| + | padding: 0; | ||
| + | margin: 0; | ||
| + | } | ||
| + | |||
| + | .menu { | ||
| + | height: 58px; | ||
| + | margin-top: 20px; | ||
| + | } | ||
| + | |||
| + | .menu li { | ||
| + | background-color: #0C0000; | ||
| + | border-bottom: 2px solid #181818; | ||
| + | border-top: 2px solid #303030; | ||
| + | min-width:16.66%; | ||
| + | } | ||
| + | |||
| + | .menu > li { | ||
| + | display: block; | ||
| + | float: left; | ||
| + | position: relative; | ||
| + | } | ||
| + | |||
| + | |||
| + | |||
| + | .menu > li:first-child { | ||
| + | border-radius: 25px 0 0 0; | ||
| + | } | ||
| + | .menu > li:last-child { | ||
| + | border-radius: 0 25px 0 0; | ||
| + | } | ||
| + | |||
| + | |||
| + | |||
| + | .menu a { | ||
| + | border-left: 3px solid rgba(0, 0, 0, 0); | ||
| + | /*background: url("../img/back.jpg");*/ | ||
| + | color: #808080; | ||
| + | display: block; | ||
| + | font-size: 18px; | ||
| + | line-height: 54px; | ||
| + | padding: 0 25px; | ||
| + | text-decoration: none; | ||
| + | text-transform: uppercase; | ||
| + | } | ||
| + | |||
| + | .menu li:hover { | ||
| + | background-color: #1c1c1c; | ||
| + | background: -moz-linear-gradient(#1c1c1c, #1b1b1b); | ||
| + | background: -ms-linear-gradient(#1c1c1c, #1b1b1b); | ||
| + | background: -webkit-gradient(linear, left top, left bottom, color-stop(0%, #1c1c1c), color-stop(100%, #1b1b1b)); | ||
| + | background: -webkit-linear-gradient(#1c1c1c, #1b1b1b); | ||
| + | background: -o-linear-gradient(#1c1c1c, #1b1b1b); | ||
| + | filter: progid:DXImageTransform.Microsoft.gradient(startColorstr='#1c1c1c', endColorstr='#1b1b1b'); | ||
| + | -ms-filter: "progid:DXImageTransform.Microsoft.gradient(startColorstr='#1c1c1c', endColorstr='#1b1b1b')"; | ||
| + | background: linear-gradient(#1c1c1c, #1b1b1b); | ||
| + | border-bottom: 2px solid #222222; | ||
| + | border-top: 2px solid #1B1B1B; | ||
| + | /*改变加深父元素的背景*/ | ||
| + | } | ||
| + | |||
| + | |||
| + | .xyz:hover > a { | ||
| + | border-radius: 25px 0 0 0 !important; | ||
| + | border-left: 3px solid #C4302B; | ||
| + | color: #C4302B; | ||
| + | } | ||
| + | .menu li:hover > a { | ||
| + | border-radius: 5px 0 0 0; | ||
| + | border-left: 3px solid #C4302B; | ||
| + | color: #C4302B; | ||
| + | } | ||
| + | |||
| + | .submenu { | ||
| + | left: 0; | ||
| + | max-height: 0; | ||
| + | position: absolute; | ||
| + | top: 100%; | ||
| + | z-index: 0; | ||
| + | -webkit-perspective: 400px; | ||
| + | -moz-perspective: 400px; | ||
| + | -ms-perspective: 400px; | ||
| + | -o-perspective: 400px; | ||
| + | perspective: 400px; | ||
| + | } | ||
| + | |||
| + | .submenu li { | ||
| + | opacity: 0; | ||
| + | -webkit-transform: rotateY(90deg); | ||
| + | -moz-transform: rotateY(90deg); | ||
| + | -ms-transform: rotateY(90deg); | ||
| + | -o-transform: rotateY(90deg); | ||
| + | transform: rotateY(90deg); | ||
| + | -webkit-transition: opacity .4s, -webkit-transform .5s; | ||
| + | -moz-transition: opacity .4s, -moz-transform .5s; | ||
| + | -ms-transition: opacity .4s, -ms-transform .5s; | ||
| + | -o-transition: opacity .4s, -o-transform .5s; | ||
| + | transition: opacity .4s, transform .5s; | ||
| + | /*实现下拉菜单螺旋淡出*/ | ||
| + | } | ||
| + | |||
| + | .menu .submenu li:hover a { | ||
| + | border-left: 3px solid #454545; | ||
| + | border-radius: 0; | ||
| + | color: #ffffff; | ||
| + | } | ||
| + | |||
| + | .menu > li:hover .submenu, .menu > li:focus .submenu { | ||
| + | max-height: 2000px; | ||
| + | z-index: 10; | ||
| + | } | ||
| + | |||
| + | .menu > li:hover .submenu li, .menu > li:focus .submenu li { | ||
| + | opacity: 1; | ||
| + | -webkit-transform: none; | ||
| + | -moz-transform: none; | ||
| + | -ms-transform: none; | ||
| + | -o-transform: none; | ||
| + | transform: none; | ||
| + | } | ||
| + | /*动画递进的时间 实现一个个螺旋子菜单*/ | ||
| + | .menu li:hover .submenu li:nth-child(1) { | ||
| + | -webkit-transition-delay: 0s; | ||
| + | -moz-transition-delay: 0s; | ||
| + | -ms-transition-delay: 0s; | ||
| + | -o-transition-delay: 0s; | ||
| + | transition-delay: 0s; | ||
| + | } | ||
| + | |||
| + | .menu li:hover .submenu li:nth-child(2) { | ||
| + | -webkit-transition-delay: 50ms; | ||
| + | -moz-transition-delay: 50ms; | ||
| + | -ms-transition-delay: 50ms; | ||
| + | -o-transition-delay: 50ms; | ||
| + | transition-delay: 50ms; | ||
| + | } | ||
| + | |||
| + | .menu li:hover .submenu li:nth-child(3) { | ||
| + | -webkit-transition-delay: 100ms; | ||
| + | -moz-transition-delay: 100ms; | ||
| + | -ms-transition-delay: 100ms; | ||
| + | -o-transition-delay: 100ms; | ||
| + | transition-delay: 100ms; | ||
| + | } | ||
| + | |||
| + | .menu li:hover .submenu li:nth-child(4) { | ||
| + | -webkit-transition-delay: 150ms; | ||
| + | -moz-transition-delay: 150ms; | ||
| + | -ms-transition-delay: 150ms; | ||
| + | -o-transition-delay: 150ms; | ||
| + | transition-delay: 150ms; | ||
| + | } | ||
| + | |||
| + | .menu li:hover .submenu li:nth-child(5) { | ||
| + | -webkit-transition-delay: 200ms; | ||
| + | -moz-transition-delay: 200ms; | ||
| + | -ms-transition-delay: 200ms; | ||
| + | -o-transition-delay: 200ms; | ||
| + | transition-delay: 200ms; | ||
| + | } | ||
| + | |||
| + | .menu li:hover .submenu li:nth-child(6) { | ||
| + | -webkit-transition-delay: 250ms; | ||
| + | -moz-transition-delay: 250ms; | ||
| + | -ms-transition-delay: 250ms; | ||
| + | -o-transition-delay: 250ms; | ||
| + | transition-delay: 250ms; | ||
| + | } | ||
| + | |||
| + | .menu li:hover .submenu li:nth-child(7) { | ||
| + | -webkit-transition-delay: 300ms; | ||
| + | -moz-transition-delay: 300ms; | ||
| + | -ms-transition-delay: 300ms; | ||
| + | -o-transition-delay: 300ms; | ||
| + | transition-delay: 300ms; | ||
| + | } | ||
| + | |||
| + | .menu li:hover .submenu li:nth-child(8) { | ||
| + | -webkit-transition-delay: 350ms; | ||
| + | -moz-transition-delay: 350ms; | ||
| + | -ms-transition-delay: 350ms; | ||
| + | -o-transition-delay: 350ms; | ||
| + | transition-delay: 350ms; | ||
| + | } | ||
| + | |||
| + | .submenu li:nth-child(1) { | ||
| + | -webkit-transition-delay: 350ms; | ||
| + | -moz-transition-delay: 350ms; | ||
| + | -ms-transition-delay: 350ms; | ||
| + | -o-transition-delay: 350ms; | ||
| + | transition-delay: 350ms; | ||
| + | } | ||
| + | |||
| + | .submenu li:nth-child(2) { | ||
| + | -webkit-transition-delay: 300ms; | ||
| + | -moz-transition-delay: 300ms; | ||
| + | -ms-transition-delay: 300ms; | ||
| + | -o-transition-delay: 300ms; | ||
| + | transition-delay: 300ms; | ||
| + | } | ||
| + | |||
| + | .submenu li:nth-child(3) { | ||
| + | -webkit-transition-delay: 250ms; | ||
| + | -moz-transition-delay: 250ms; | ||
| + | -ms-transition-delay: 250ms; | ||
| + | -o-transition-delay: 250ms; | ||
| + | transition-delay: 250ms; | ||
| + | } | ||
| + | |||
| + | .submenu li:nth-child(4) { | ||
| + | -webkit-transition-delay: 200ms; | ||
| + | -moz-transition-delay: 200ms; | ||
| + | -ms-transition-delay: 200ms; | ||
| + | -o-transition-delay: 200ms; | ||
| + | transition-delay: 200ms; | ||
| + | } | ||
| + | |||
| + | .submenu li:nth-child(5) { | ||
| + | -webkit-transition-delay: 150ms; | ||
| + | -moz-transition-delay: 150ms; | ||
| + | -ms-transition-delay: 150ms; | ||
| + | -o-transition-delay: 150ms; | ||
| + | transition-delay: 150ms; | ||
| + | } | ||
| + | |||
| + | .submenu li:nth-child(6) { | ||
| + | -webkit-transition-delay: 100ms; | ||
| + | -moz-transition-delay: 100ms; | ||
| + | -ms-transition-delay: 100ms; | ||
| + | -o-transition-delay: 100ms; | ||
| + | transition-delay: 100ms; | ||
| + | } | ||
| + | |||
| + | .submenu li:nth-child(7) { | ||
| + | -webkit-transition-delay: 50ms; | ||
| + | -moz-transition-delay: 50ms; | ||
| + | -ms-transition-delay: 50ms; | ||
| + | -o-transition-delay: 50ms; | ||
| + | transition-delay: 50ms; | ||
| + | } | ||
| + | |||
| + | .submenu li:nth-child(8) { | ||
| + | -webkit-transition-delay: 0s; | ||
| + | -moz-transition-delay: 0s; | ||
| + | -ms-transition-delay: 0s; | ||
| + | -o-transition-delay: 0s; | ||
| + | transition-delay: 0s; | ||
| + | } | ||
| + | |||
| + | </style> | ||
| + | |||
| + | <div class="nav"> | ||
| + | <ul class="menu"> | ||
| + | <li class="xyz"><a href="https://2017.igem.org/Team:FAFU-CHINA">HOME</a></li> | ||
| + | <li><a href="javascript:;">PROJECT</a> | ||
| + | <ul class="submenu"> | ||
| + | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/Description">Description</a></li> | ||
| + | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/Experiments">Experiments</a></li> | ||
| + | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/Results">Results</a></li> | ||
| + | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/Model">Model</a></li> | ||
| + | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/Parts">Parts</a></li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/Human_Practices">Human Practices</a></li> | ||
| + | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/Safety">Safety</a></li> | ||
| + | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/Notebook">Notebook</a></li> | ||
| + | <li><a href="https://2017.igem.org/Team:FAFU-CHINA/Team">Team</a></li> | ||
| + | </ul> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
<style> | <style> | ||
| − | + | /*file has been compressed and all comments has been deleted for security reasons */ | |
| − | + | ||
| − | + | /* GLOBAL STYLES | |
| − | + | ===================================================================*/ | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | html,body{ | |
| − | + | margin:0; | |
| − | + | padding:0; | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
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} | } | ||
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| − | |||
| − | |||
| − | |||
| − | |||
| − | + | ||
| − | + | @font-face { | |
| − | + | font-family: 'OswaldLight'; | |
| − | + | font-weight: normal; | |
| − | + | font-style: normal; | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
} | } | ||
| − | . | + | .container{ position: relative; width: 960px; margin: 0 auto; padding: 0; } |
| − | + | </style> | |
| − | + | ||
| − | + | <style> | |
| − | + | ||
| − | + | .slides,.flex-control-nav,.flex-direction-nav{ | |
| − | + | margin:0; | |
| − | + | padding:0; | |
| − | + | list-style:none; | |
| − | + | ||
| − | + | ||
} | } | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | + | ||
| − | + | .slides:after{ | |
| − | + | content:"."; | |
| − | + | display:block; | |
| − | + | clear:both; | |
| − | + | visibility:hidden; | |
| − | + | line-height:0; | |
| + | height:0; | ||
} | } | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | + | ||
| − | + | .flex-container{ | |
| − | + | zoom:1; | |
| − | + | position:relative; | |
| − | + | ||
} | } | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | + | .flex-direction-nav li a{ | |
| − | + | margin:0; | |
| − | + | display:block; | |
| − | - | + | position:absolute; |
| − | + | top:45%; | |
| + | cursor:pointer; | ||
| + | z-index:150; | ||
| + | background-color: #2F343A; | ||
| + | height: 38px; | ||
| + | text-indent: -9999px; | ||
| + | width: 38px; | ||
| + | padding:8px; | ||
} | } | ||
| − | . | + | |
| − | + | .flex-direction-nav li a:hover { | |
| − | + | opacity:1; filter:alpha(opacity=100); | |
| − | + | background-color: #ffea00; | |
} | } | ||
| − | . | + | |
| − | + | .flex-direction-nav li .flex-next{ | |
| − | + | right:0; | |
| + | background-image: url("https://static.igem.org/mediawiki/2017/b/b1/T--FAFU-CHINA--next-small.png"); | ||
| + | background-position: center center; | ||
| + | background-repeat: no-repeat; | ||
} | } | ||
| − | |||
| − | |||
| − | + | .flex-direction-nav li .flex-prev{ | |
| − | + | left:0; | |
| − | + | background-image: url("https://static.igem.org/mediawiki/2017/5/5a/T--FAFU-CHINA--prev-small.png"); | |
| − | - | + | background-position: center center; |
| − | + | background-repeat: no-repeat; | |
} | } | ||
| − | . | + | .flex-direction-nav li .flex-next:hover{ |
| − | + | background-image: url("https://static.igem.org/mediawiki/2017/9/9e/T--FAFU-CHINA--next-small-black.png"); | |
| − | + | background-position: center center; | |
| − | + | background-repeat: no-repeat; | |
| − | - | + | |
| − | + | ||
} | } | ||
| − | . | + | |
| − | + | .flex-direction-nav li .flex-prev:hover{ | |
| − | + | background-image: url("https://static.igem.org/mediawiki/2017/9/9a/T--FAFU-CHINA--prev-small-black.png"); | |
| − | + | background-position: center center; | |
| − | - | + | background-repeat: no-repeat; |
| − | + | ||
} | } | ||
| − | . | + | |
| − | + | .flex-direction-nav li .disabled{ | |
| − | + | opacity:.3; | |
| − | + | filter:alpha(opacity=30); | |
| − | + | cursor:default; | |
| − | + | ||
} | } | ||
| − | . | + | |
| − | + | .slider-1 .flex-direction-nav li .flex-next{ | |
| − | + | position:absolute; | |
| − | + | right:-30px; | |
| − | + | opacity:0; filter:alpha(opacity=0); | |
| − | + | -webkit-transition: all .3s ease; | |
} | } | ||
| − | . | + | |
| − | + | .slider-1:hover .flex-direction-nav li .flex-next { | |
| − | + | opacity:1; filter:alpha(opacity=100); | |
| − | + | right:0; | |
| − | + | ||
| − | + | ||
} | } | ||
| − | . | + | |
| − | + | .slider-1 .flex-direction-nav li .flex-prev{ | |
| − | + | position:absolute; | |
| − | + | left:-30px; | |
| − | + | opacity:0; filter:alpha(opacity=0); | |
| − | + | -webkit-transition: all .3s ease; | |
} | } | ||
| − | . | + | |
| − | + | .slider-1:hover .flex-direction-nav li .flex-prev{ | |
| − | + | opacity:1; filter:alpha(opacity=100); | |
| − | + | left:0; | |
| − | + | ||
| − | + | ||
} | } | ||
| − | . | + | |
| − | + | .flex-control-nav{ | |
| − | + | width:100%; | |
| − | + | position:absolute; | |
| − | + | bottom:-32px; | |
| − | + | text-align:center; | |
| + | -webkit-transition: all .3s ease; | ||
} | } | ||
| − | . | + | .slider-1:hover .flex-control-nav { |
| − | + | bottom:18px; | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
} | } | ||
| − | . | + | |
| − | + | .flex-control-nav li{ | |
| − | + | margin:0 0 0 5px; | |
| − | + | display:inline-block; | |
| − | + | zoom:1; | |
| − | + | *display:inline; | |
} | } | ||
| − | . | + | |
| − | + | .flex-control-nav li:first-child{ | |
| − | + | margin:0; | |
| − | + | ||
| − | + | ||
| − | + | ||
} | } | ||
| − | . | + | |
| − | + | .slider-1 { | |
| − | + | overflow:hidden; | |
| − | + | ||
| − | + | ||
| − | + | ||
} | } | ||
| − | . | + | |
| − | + | .slider-1 .slides li .contain, .slider-1 .loading { | |
| − | + | position:relative; | |
| − | + | min-height:666px; | |
| − | + | ||
| − | + | ||
} | } | ||
| − | . | + | |
| − | + | .slider-1 .slides li h2, .slider-1 .slides li p, .slider-1 .slides li .links { | |
| − | + | max-width:480px; | |
| − | + | min-width:480px; | |
| − | + | ||
| − | + | ||
} | } | ||
| − | . | + | |
| − | + | .slider-1 .slides li h2, .slider-1 .slides li p, .slider-1 .slides li .links, .slider-1 .slides li .item { | |
| − | + | position:absolute; | |
| − | + | ||
| − | + | ||
| − | + | ||
} | } | ||
| − | . | + | |
| − | + | .slider-1 .slides li h2 { | |
| − | + | left:0; | |
| − | + | top:-100%; | |
| − | + | opacity:0; | |
| − | + | z-index:50; | |
| + | font-family: 'OswaldLight', Arial, Helvetica, sans-serif; | ||
| + | font-size:12px;/*黄色区域中的字体*/ | ||
| + | /* line-height:56px;*/ | ||
| + | min-width:50px; | ||
| + | color:#414146; | ||
| + | background-color:#ffea00; | ||
| + | display: inline-block !important; | ||
| + | padding: 5px 5px 5px; | ||
| + | margin:0; | ||
| + | |||
| + | } | ||
| + | .slider-1 .slides li p { | ||
| + | left:0; | ||
| + | bottom:-50%; | ||
| + | opacity:0; | ||
| + | z-index:50; | ||
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| − | + | .slider-1.iphone li img { | |
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} | } | ||
| − | . | + | |
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| − | + | </style> | |
| − | + | <!-- SLIDER --> | |
| − | + | <div class="slider-1 clearfix"> | |
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| − | } | + | <div class="flex-container"> |
| − | + | <div class="flexslider loading"> | |
| + | <ul class="slides"> | ||
| + | <li> | ||
| + | |||
| + | <div class="container"> | ||
| + | <div class="sixteen columns contain"> | ||
| + | |||
| + | <h2 data-toptitle="12%"> Continued worldwide industrialization has caused extensive environmental and human health problems. A wide variety of chemicals, e.g., heavy metals, pesticides, chlorinated solvents, etc., have been detected in different natural resources such as soil, water, and air. Among the pollutants, the heavy metals are of concern to human health due to their cytotoxicity,mutagenicity, and carcinogenicity.<br/> | ||
| + | Soil polluted by heavy metals represent an important environmental problem due to the toxic effects of metals, their accumulations throughout the food chain and the additional risk of groundwater contamination.<br/> | ||
| + | Metal remediation through common physico-chemical techniques is expensive and unsuitable in the case of extensive areas. Therefore biotechnological approaches have received a great deal of attention in recent years. Bioremediaton, as a way of natural attenuation, adds phytoremediation, the use of plants for reclamation and microbe(PSB) together as a strategy for either enhancing uptake(PSB-assisted | ||
| + | Phytoextraction) or reducing the bioavailability of metal contaminants(PSB-immobilized Phyto-promotion mechanism) in the soil, especially in the rhizosphere to accelerate plant biomass production and root enlongation.</h2> | ||
| + | <p data-bottomtext="32%">overvie</p> | ||
| + | <!-- <div class="links" data-bottomlinks="30%"> | ||
| + | <a class="button medium r-m-plus r-m-full" href="blog-single.html">READ MORE</a> | ||
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| + | |||
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| + | |||
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| + | |||
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| + | |||
| + | <div class="container"> | ||
| + | <div class="sixteen columns contain"> | ||
| + | |||
| + | <h2 data-toptitle="17%"> Phytoextraction is based on the use of hyperaccumulator plants, which can tolerate and accumulate high concentration of metals. Ideal hyperaccumulators require the characteristics of deep rooted, rapid growth and a high amount of biomass. In fact, many hyperaccumulators are slow in growth, produce low bow biomass and cannot grow in metal-stress soil. Further, the overproduction of ethylene in response to metal-stress leads to inhibition of root growth, root hair formation and consequently development of the plant as a whole. Therefore, in our project we took advantage of co-expression of ACC deaminase and iaaM genes in our chassis PSB(B. megaterium) to enhance plant growth and biomass by P solubilization and uptake particularly under stress condition by heavy metals. Meanwhile, we also exploited bacterial chemotaxis, McfS, a multiple chemoreceptor from strain P. putida F1 to detect response of organic acids secreting in the rhizosphere.</h2> | ||
| + | <p data-bottomtext="32%">PSB-assisted Phytoextraction(ex situ)</p> | ||
| + | <!-- <div class="links" data-bottomlinks="30%"> | ||
| + | <a class="button medium r-m-plus r-m-full" href="blog-single.html">READ MORE</a> | ||
| + | </div>--> | ||
| + | <img src="https://static.igem.org/mediawiki/2017/7/78/T--FAFU-CHINA--A.png" class="item" alt="slide-item" data-topimage="21%"/> | ||
| + | </div> | ||
| + | </div><!-- End Container --> | ||
| + | |||
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| + | |||
| + | |||
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| + | |||
| + | <div class="container"> | ||
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| + | |||
| + | <h2 data-toptitle="17%"> In this module, our team designed a pathway that the Pb ions will bind to PbR protein when activated ions are in the soil environment. It induces downstream gene expression, and results in the tetR protein inhibition promoter PltetO1 expression. At the same time, the strong promoter P43 of B.megaterium stimulated the expression of MBP in the downstream lead-producing peptide. It makes the chelating peptide of Pb metal, which reduced the total amount of heavy metals in the soil to be harmless level. When there is no Pb ions presented in the environment, the expression of tetR was blocked and the inhibition of PltetO1 was released. It results in the lacI repressor binding to the lacO sequence, and leads to blocking the expression of MBP. This mechanism is effective in preventing the infinite expression of MBP against the B. megaterium itself.</h2> | ||
| + | <p data-bottomtext="32%">PSB-immobilized Phyto-promotion</p> | ||
| + | <!-- <div class="links" data-bottomlinks="30%"> | ||
| + | <a class="button medium r-m-plus r-m-full" href="blog-single.html">READ MORE</a> | ||
| + | </div> | ||
| + | |||
| + | <img src="images/sliders/slider-1/slider-item-2.png" class="item" alt="slide-item" data-topimage="24%"/>--> | ||
| + | </div> | ||
| + | </div><!-- End Container --> | ||
| + | |||
| + | </li><!-- End item --> | ||
| + | |||
| + | |||
| + | </ul> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
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| + | |||
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e=="string"){t=e.split(y);for(n=0,r=this.length;n<r;n++){i=this[n];if(i.nodeType===1)if(!i.className&&t.length===1)i.className=e;else{s=" "+i.className+" ";for(o=0,u=t.length;o<u;o++)s.indexOf(" "+t[o]+" ")<0&&(s+=t[o]+" ");i.className=v.trim(s)}}}return this},removeClass:function(e){var n,r,i,s,o,u,a;if(v.isFunction(e))return this.each(function(t){v(this).removeClass(e.call(this,t,this.className))});if(e&&typeof e=="string"||e===t){n=(e||"").split(y);for(u=0,a=this.length;u<a;u++){i=this[u];if(i.nodeType===1&&i.className){r=(" "+i.className+" ").replace(q," ");for(s=0,o=n.length;s<o;s++)while(r.indexOf(" "+n[s]+" ")>=0)r=r.replace(" "+n[s]+" "," ");i.className=e?v.trim(r):""}}}return this},toggleClass:function(e,t){var n=typeof e,r=typeof t=="boolean";return v.isFunction(e)?this.each(function(n){v(this).toggleClass(e.call(this,n,this.className,t),t)}):this.each(function(){if(n==="string"){var 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n=="object"?n[v.expando]?n:new v.Event(y,n):new v.Event(y),n.type=y,n.isTrigger=!0,n.exclusive=a,n.namespace=b.join("."),n.namespace_re=n.namespace?new RegExp("(^|\\.)"+b.join("\\.(?:.*\\.|)")+"(\\.|$)"):null,h=y.indexOf(":")<0?"on"+y:"";if(!s){u=v.cache;for(f in u)u[f].events&&u[f].events[y]&&v.event.trigger(n,r,u[f].handle.elem,!0);return}n.result=t,n.target||(n.target=s),r=r!=null?v.makeArray(r):[],r.unshift(n),p=v.event.special[y]||{};if(p.trigger&&p.trigger.apply(s,r)===!1)return;m=[[s,p.bindType||y]];if(!o&&!p.noBubble&&!v.isWindow(s)){g=p.delegateType||y,l=Y.test(g+y)?s:s.parentNode;for(c=s;l;l=l.parentNode)m.push([l,g]),c=l;c===(s.ownerDocument||i)&&m.push([c.defaultView||c.parentWindow||e,g])}for(f=0;f<m.length&&!n.isPropagationStopped();f++)l=m[f][0],n.type=m[f][1],d=(v._data(l,"events")||{})[n.type]&&v._data(l,"handle"),d&&d.apply(l,r),d=h&&l[h],d&&v.acceptData(l)&&d.apply&&d.apply(l,r)===!1&&n.preventDefault();return n.type=y,!o&&!n.isDefaultPrevented()&&(!p._default||p._default.apply(s.ownerDocument,r)===!1)&&(y!=="click"||!v.nodeName(s,"a"))&&v.acceptData(s)&&h&&s[y]&&(y!=="focus"&&y!=="blur"||n.target.offsetWidth!==0)&&!v.isWindow(s)&&(c=s[h],c&&(s[h]=null),v.event.triggered=y,s[y](),v.event.triggered=t,c&&(s[h]=c)),n.result}return},dispatch:function(n){n=v.event.fix(n||e.event);var r,i,s,o,u,a,f,c,h,p,d=(v._data(this,"events")||{})[n.type]||[],m=d.delegateCount,g=l.call(arguments),y=!n.exclusive&&!n.namespace,b=v.event.special[n.type]||{},w=[];g[0]=n,n.delegateTarget=this;if(b.preDispatch&&b.preDispatch.call(this,n)===!1)return;if(m&&(!n.button||n.type!=="click"))for(s=n.target;s!=this;s=s.parentNode||this)if(s.disabled!==!0||n.type!=="click"){u={},f=[];for(r=0;r<m;r++)c=d[r],h=c.selector,u[h]===t&&(u[h]=c.needsContext?v(h,this).index(s)>=0:v.find(h,this,null,[s]).length),u[h]&&f.push(c);f.length&&w.push({elem:s,matches:f})}d.length>m&&w.push({elem:this,matches:d.slice(m)});for(r=0;r<w.length&&!n.isPropagationStopped();r++){a=w[r],n.currentTarget=a.elem;for(i=0;i<a.matches.length&&!n.isImmediatePropagationStopped();i++){c=a.matches[i];if(y||!n.namespace&&!c.namespace||n.namespace_re&&n.namespace_re.test(c.namespace))n.data=c.data,n.handleObj=c,o=((v.event.special[c.origType]||{}).handle||c.handler).apply(a.elem,g),o!==t&&(n.result=o,o===!1&&(n.preventDefault(),n.stopPropagation()))}}return 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i=v.extend(new v.Event,n,{type:e,isSimulated:!0,originalEvent:{}});r?v.event.trigger(i,null,t):v.event.dispatch.call(t,i),i.isDefaultPrevented()&&n.preventDefault()}},v.event.handle=v.event.dispatch,v.removeEvent=i.removeEventListener?function(e,t,n){e.removeEventListener&&e.removeEventListener(t,n,!1)}:function(e,t,n){var r="on"+t;e.detachEvent&&(typeof e[r]=="undefined"&&(e[r]=null),e.detachEvent(r,n))},v.Event=function(e,t){if(!(this instanceof v.Event))return new v.Event(e,t);e&&e.type?(this.originalEvent=e,this.type=e.type,this.isDefaultPrevented=e.defaultPrevented||e.returnValue===!1||e.getPreventDefault&&e.getPreventDefault()?tt:et):this.type=e,t&&v.extend(this,t),this.timeStamp=e&&e.timeStamp||v.now(),this[v.expando]=!0},v.Event.prototype={preventDefault:function(){this.isDefaultPrevented=tt;var e=this.originalEvent;if(!e)return;e.preventDefault?e.preventDefault():e.returnValue=!1},stopPropagation:function(){this.isPropagationStopped=tt;var e=this.originalEvent;if(!e)return;e.stopPropagation&&e.stopPropagation(),e.cancelBubble=!0},stopImmediatePropagation:function(){this.isImmediatePropagationStopped=tt,this.stopPropagation()},isDefaultPrevented:et,isPropagationStopped:et,isImmediatePropagationStopped:et},v.each({mouseenter:"mouseover",mouseleave:"mouseout"},function(e,t){v.event.special[e]={delegateType:t,bindType:t,handle:function(e){var n,r=this,i=e.relatedTarget,s=e.handleObj,o=s.selector;if(!i||i!==r&&!v.contains(r,i))e.type=s.origType,n=s.handler.apply(this,arguments),e.type=t;return n}}}),v.support.submitBubbles||(v.event.special.submit={setup:function(){if(v.nodeName(this,"form"))return!1;v.event.add(this,"click._submit keypress._submit",function(e){var n=e.target,r=v.nodeName(n,"input")||v.nodeName(n,"button")?n.form:t;r&&!v._data(r,"_submit_attached")&&(v.event.add(r,"submit._submit",function(e){e._submit_bubble=!0}),v._data(r,"_submit_attached",!0))})},postDispatch:function(e){e._submit_bubble&&(delete 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o=arguments.length&&(r||typeof i!="boolean"),u=r||(i===!0||s===!0?"margin":"border");return v.access(this,function(n,r,i){var s;return v.isWindow(n)?n.document.documentElement["client"+e]:n.nodeType===9?(s=n.documentElement,Math.max(n.body["scroll"+e],s["scroll"+e],n.body["offset"+e],s["offset"+e],s["client"+e])):i===t?v.css(n,r,i,u):v.style(n,r,i,u)},n,o?i:t,o,null)}})}),e.jQuery=e.$=v,typeof define=="function"&&define.amd&&define.amd.jQuery&&define("jquery",[],function(){return v})})(window); | ||
| + | |||
| + | var $j = jQuery.noConflict(); | ||
| + | </script> | ||
| + | |||
| + | <!-- Flex Slider --> | ||
| + | <script> | ||
| + | /* | ||
| + | * jQuery FlexSlider v2.2.0 | ||
| + | * Copyright 2012 WooThemes | ||
| + | * Contributing Author: Tyler Smith | ||
| + | */(function(e){e.flexslider=function(t,n){var r=e(t);r.vars=e.extend({},e.flexslider.defaults,n);var i=r.vars.namespace,s=window.navigator&&window.navigator.msPointerEnabled&&window.MSGesture,o=("ontouchstart"in window||s||window.DocumentTouch&&document instanceof DocumentTouch)&&r.vars.touch,u="click touchend MSPointerUp",a="",f,l=r.vars.direction==="vertical",c=r.vars.reverse,h=r.vars.itemWidth>0,p=r.vars.animation==="fade",d=r.vars.asNavFor!=="",v={},m=!0;e.data(t,"flexslider",r);v={init:function(){r.animating=!1;r.currentSlide=parseInt(r.vars.startAt?r.vars.startAt:0);isNaN(r.currentSlide)&&(r.currentSlide=0);r.animatingTo=r.currentSlide;r.atEnd=r.currentSlide===0||r.currentSlide===r.last;r.containerSelector=r.vars.selector.substr(0,r.vars.selector.search(" 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s=t<0?r.getTarget("next"):r.getTarget("prev");r.flexAnimate(s,r.vars.pauseOnAction)});r.vars.pausePlay&&v.pausePlay.setup();r.vars.slideshow&&r.vars.pauseInvisible&&v.pauseInvisible.init();if(r.vars.slideshow){r.vars.pauseOnHover&&r.hover(function(){!r.manualPlay&&!r.manualPause&&r.pause()},function(){!r.manualPause&&!r.manualPlay&&!r.stopped&&r.play()});if(!r.vars.pauseInvisible||!v.pauseInvisible.isHidden())r.vars.initDelay>0?r.startTimeout=setTimeout(r.play,r.vars.initDelay):r.play()}d&&v.asNav.setup();o&&r.vars.touch&&v.touch();(!p||p&&r.vars.smoothHeight)&&e(window).bind("resize orientationchange focus",v.resize);r.find("img").attr("draggable","false");setTimeout(function(){r.vars.start(r)},200)},asNav:{setup:function(){r.asNav=!0;r.animatingTo=Math.floor(r.currentSlide/r.move);r.currentItem=r.currentSlide;r.slides.removeClass(i+"active-slide").eq(r.currentItem).addClass(i+"active-slide");if(!s)r.slides.click(function(t){t.preventDefault();var n=e(this),s=n.index(),o=n.offset().left-e(r).scrollLeft();if(o<=0&&n.hasClass(i+"active-slide"))r.flexAnimate(r.getTarget("prev"),!0);else if(!e(r.vars.asNavFor).data("flexslider").animating&&!n.hasClass(i+"active-slide")){r.direction=r.currentItem<s?"next":"prev";r.flexAnimate(s,r.vars.pauseOnAction,!1,!0,!0)}});else{t._slider=r;r.slides.each(function(){var t=this;t._gesture=new MSGesture;t._gesture.target=t;t.addEventListener("MSPointerDown",function(e){e.preventDefault();e.currentTarget._gesture&&e.currentTarget._gesture.addPointer(e.pointerId)},!1);t.addEventListener("MSGestureTap",function(t){t.preventDefault();var n=e(this),i=n.index();if(!e(r.vars.asNavFor).data("flexslider").animating&&!n.hasClass("active")){r.direction=r.currentItem<i?"next":"prev";r.flexAnimate(i,r.vars.pauseOnAction,!1,!0,!0)}})})}}},controlNav:{setup:function(){r.manualControls?v.controlNav.setupManual():v.controlNav.setupPaging()},setupPaging:function(){var t=r.vars.controlNav==="thumbnails"?"control-thumbs":"control-paging",n=1,s,o;r.controlNavScaffold=e('<ol class="'+i+"control-nav "+i+t+'"></ol>');if(r.pagingCount>1)for(var f=0;f<r.pagingCount;f++){o=r.slides.eq(f);s=r.vars.controlNav==="thumbnails"?'<img src="'+o.attr("data-thumb")+'"/>':"<a>"+n+"</a>";if("thumbnails"===r.vars.controlNav&&!0===r.vars.thumbCaptions){var l=o.attr("data-thumbcaption");""!=l&&undefined!=l&&(s+='<span class="'+i+'caption">'+l+"</span>")}r.controlNavScaffold.append("<li>"+s+"</li>");n++}r.controlsContainer?e(r.controlsContainer).append(r.controlNavScaffold):r.append(r.controlNavScaffold);v.controlNav.set();v.controlNav.active();r.controlNavScaffold.delegate("a, img",u,function(t){t.preventDefault();if(a===""||a===t.type){var n=e(this),s=r.controlNav.index(n);if(!n.hasClass(i+"active")){r.direction=s>r.currentSlide?"next":"prev";r.flexAnimate(s,r.vars.pauseOnAction)}}a===""&&(a=t.type);v.setToClearWatchedEvent()})},setupManual:function(){r.controlNav=r.manualControls;v.controlNav.active();r.controlNav.bind(u,function(t){t.preventDefault();if(a===""||a===t.type){var n=e(this),s=r.controlNav.index(n);if(!n.hasClass(i+"active")){s>r.currentSlide?r.direction="next":r.direction="prev";r.flexAnimate(s,r.vars.pauseOnAction)}}a===""&&(a=t.type);v.setToClearWatchedEvent()})},set:function(){var t=r.vars.controlNav==="thumbnails"?"img":"a";r.controlNav=e("."+i+"control-nav li 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n;if(a===""||a===t.type){n=e(this).hasClass(i+"next")?r.getTarget("next"):r.getTarget("prev");r.flexAnimate(n,r.vars.pauseOnAction)}a===""&&(a=t.type);v.setToClearWatchedEvent()})},update:function(){var e=i+"disabled";r.pagingCount===1?r.directionNav.addClass(e).attr("tabindex","-1"):r.vars.animationLoop?r.directionNav.removeClass(e).removeAttr("tabindex"):r.animatingTo===0?r.directionNav.removeClass(e).filter("."+i+"prev").addClass(e).attr("tabindex","-1"):r.animatingTo===r.last?r.directionNav.removeClass(e).filter("."+i+"next").addClass(e).attr("tabindex","-1"):r.directionNav.removeClass(e).removeAttr("tabindex")}},pausePlay:{setup:function(){var t=e('<div class="'+i+'pauseplay"><a></a></div>');if(r.controlsContainer){r.controlsContainer.append(t);r.pausePlay=e("."+i+"pauseplay a",r.controlsContainer)}else{r.append(t);r.pausePlay=e("."+i+"pauseplay a",r)}v.pausePlay.update(r.vars.slideshow?i+"pause":i+"play");r.pausePlay.bind(u,function(t){t.preventDefault();if(a===""||a===t.type)if(e(this).hasClass(i+"pause")){r.manualPause=!0;r.manualPlay=!1;r.pause()}else{r.manualPause=!1;r.manualPlay=!0;r.play()}a===""&&(a=t.type);v.setToClearWatchedEvent()})},update:function(e){e==="play"?r.pausePlay.removeClass(i+"pause").addClass(i+"play").html(r.vars.playText):r.pausePlay.removeClass(i+"play").addClass(i+"pause").html(r.vars.pauseText)}},touch:function(){var e,n,i,o,u,a,f=!1,d=0,v=0,m=0;if(!s){t.addEventListener("touchstart",g,!1);function g(s){if(r.animating)s.preventDefault();else if(window.navigator.msPointerEnabled||s.touches.length===1){r.pause();o=l?r.h:r.w;a=Number(new Date);d=s.touches[0].pageX;v=s.touches[0].pageY;i=h&&c&&r.animatingTo===r.last?0:h&&c?r.limit-(r.itemW+r.vars.itemMargin)*r.move*r.animatingTo:h&&r.currentSlide===r.last?r.limit:h?(r.itemW+r.vars.itemMargin)*r.move*r.currentSlide:c?(r.last-r.currentSlide+r.cloneOffset)*o:(r.currentSlide+r.cloneOffset)*o;e=l?v:d;n=l?d:v;t.addEventListener("touchmove",y,!1);t.addEventListener("touchend",b,!1)}}function y(t){d=t.touches[0].pageX;v=t.touches[0].pageY;u=l?e-v:e-d;f=l?Math.abs(u)<Math.abs(d-n):Math.abs(u)<Math.abs(v-n);var s=500;if(!f||Number(new Date)-a>s){t.preventDefault();if(!p&&r.transitions){r.vars.animationLoop||(u/=r.currentSlide===0&&u<0||r.currentSlide===r.last&&u>0?Math.abs(u)/o+2:1);r.setProps(i+u,"setTouch")}}}function b(s){t.removeEventListener("touchmove",y,!1);if(r.animatingTo===r.currentSlide&&!f&&u!==null){var l=c?-u:u,h=l>0?r.getTarget("next"):r.getTarget("prev");r.canAdvance(h)&&(Number(new Date)-a<550&&Math.abs(l)>50||Math.abs(l)>o/2)?r.flexAnimate(h,r.vars.pauseOnAction):p||r.flexAnimate(r.currentSlide,r.vars.pauseOnAction,!0)}t.removeEventListener("touchend",b,!1);e=null;n=null;u=null;i=null}}else{t.style.msTouchAction="none";t._gesture=new MSGesture;t._gesture.target=t;t.addEventListener("MSPointerDown",w,!1);t._slider=r;t.addEventListener("MSGestureChange",E,!1);t.addEventListener("MSGestureEnd",S,!1);function w(e){e.stopPropagation();if(r.animating)e.preventDefault();else{r.pause();t._gesture.addPointer(e.pointerId);m=0;o=l?r.h:r.w;a=Number(new Date);i=h&&c&&r.animatingTo===r.last?0:h&&c?r.limit-(r.itemW+r.vars.itemMargin)*r.move*r.animatingTo:h&&r.currentSlide===r.last?r.limit:h?(r.itemW+r.vars.itemMargin)*r.move*r.currentSlide:c?(r.last-r.currentSlide+r.cloneOffset)*o:(r.currentSlide+r.cloneOffset)*o}}function E(e){e.stopPropagation();var n=e.target._slider;if(!n)return;var r=-e.translationX,s=-e.translationY;m+=l?s:r;u=m;f=l?Math.abs(m)<Math.abs(-r):Math.abs(m)<Math.abs(-s);if(e.detail===e.MSGESTURE_FLAG_INERTIA){setImmediate(function(){t._gesture.stop()});return}if(!f||Number(new Date)-a>500){e.preventDefault();if(!p&&n.transitions){n.vars.animationLoop||(u=m/(n.currentSlide===0&&m<0||n.currentSlide===n.last&&m>0?Math.abs(m)/o+2:1));n.setProps(i+u,"setTouch")}}}function S(t){t.stopPropagation();var r=t.target._slider;if(!r)return;if(r.animatingTo===r.currentSlide&&!f&&u!==null){var s=c?-u:u,l=s>0?r.getTarget("next"):r.getTarget("prev");r.canAdvance(l)&&(Number(new Date)-a<550&&Math.abs(s)>50||Math.abs(s)>o/2)?r.flexAnimate(l,r.vars.pauseOnAction):p||r.flexAnimate(r.currentSlide,r.vars.pauseOnAction,!0)}e=null;n=null;u=null;i=null;m=0}}},resize:function(){if(!r.animating&&r.is(":visible")){h||r.doMath();if(p)v.smoothHeight();else if(h){r.slides.width(r.computedW);r.update(r.pagingCount);r.setProps()}else 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e.flexslider(this,t)});var n=e(this).data("flexslider");switch(t){case"play":n.play();break;case"pause":n.pause();break;case"stop":n.stop();break;case"next":n.flexAnimate(n.getTarget("next"),!0);break;case"prev":case"previous":n.flexAnimate(n.getTarget("prev"),!0);break;default:typeof t=="number"&&n.flexAnimate(t,!0)}}})(jQuery); | ||
| + | |||
| + | var $j = jQuery.noConflict(); | ||
| + | </script> | ||
| + | <script> | ||
| + | |||
| + | $j(document).ready(function($j) { | ||
| + | |||
| + | |||
| + | |||
| + | // Flex Slider | ||
| + | $j(window).ready(function() { | ||
| + | |||
| + | $j('.flexslider').flexslider({ | ||
| + | animation: 'fade', | ||
| + | animationLoop: true, //Boolean: Should the animation loop? If false, directionNav will received "disable" classes at either end | ||
| + | slideshow: true, //Boolean: Animate slider automatically | ||
| + | slideshowSpeed: 5000, //Integer: Set the speed of the slideshow cycling, in milliseconds | ||
| + | animationSpeed: 800, //Boolean: Pause the slideshow when interacting with control elements, highly recommended. | ||
| + | pauseOnHover: true, | ||
| + | pauseOnAction:false, | ||
| + | controlNav: false, | ||
| + | directionNav: true, | ||
| + | controlsContainer: '.flex-container', | ||
| + | start: function(slider) { | ||
| + | var toptitle = $j('.slider-1 .flex-active-slide h2').data('toptitle'); | ||
| + | var topimage = $j('.slider-1 .flex-active-slide .item').data('topimage'); | ||
| + | var bottomtext = $j('.slider-1 .flex-active-slide p').data('bottomtext'); | ||
| + | var bottomlinks = $j('.slider-1 .flex-active-slide .links').data('bottomlinks'); | ||
| + | |||
| + | $j('.slider-1 .flex-active-slide').find('.item').css({ top: topimage}); | ||
| + | $j('.slider-1 .flex-active-slide').find('.item').animate({ right: '0', opacity: '1'}, 1000); | ||
| + | $j('.slider-1 .flex-active-slide').find('h2').animate({ left: '0', top: toptitle, opacity: '1'}, 1500); | ||
| + | $j('.slider-1 .flex-active-slide').find('p').animate({ left: '0', bottom: bottomtext, opacity: '1'}, 1500); | ||
| + | $j('.slider-1 .flex-active-slide').find('.links').css({ bottom: bottomlinks}); | ||
| + | $j('.slider-1 .flex-active-slide').find('.links').animate({ left: '0', opacity: '1'}, 1800); | ||
| + | |||
| + | // remove class loading after start | ||
| + | slider.removeClass('loading'); | ||
| + | }, | ||
| + | before: function(slider) { | ||
| + | $j('.slider-1 .flex-active-slide').find('.item').animate({ right: '-100%', opacity: '0'}, 1000); | ||
| + | $j('.slider-1 .flex-active-slide').find('h2').animate({ left: '0', top: '-100%', opacity: '0'}, 1500); | ||
| + | $j('.slider-1 .flex-active-slide').find('p').animate({ left: '0', bottom: '-50%', opacity: '0'}, 1500); | ||
| + | $j('.slider-1 .flex-active-slide').find('.links').animate({ left: '-100%', opacity: '0'}, 1800); | ||
| + | }, | ||
| + | after: function(slider) { | ||
| + | var toptitle = $j('.slider-1 .flex-active-slide h2').data('toptitle'); | ||
| + | var topimage = $j('.slider-1 .flex-active-slide .item').data('topimage'); | ||
| + | var bottomtext = $j('.slider-1 .flex-active-slide p').data('bottomtext'); | ||
| + | var bottomlinks = $j('.slider-1 .flex-active-slide .links').data('bottomlinks'); | ||
| + | |||
| + | $j('.slider-1 .flex-active-slide').find('.item').css({ top: topimage}); | ||
| + | $j('.slider-1 .flex-active-slide').find('.item').animate({ right: '0', opacity: '1'}, 1000); | ||
| + | $j('.slider-1 .flex-active-slide').find('h2').animate({ left: '0', top: toptitle, opacity: '1'}, 1500); | ||
| + | $j('.slider-1 .flex-active-slide').find('p').animate({ left: '0', bottom: bottomtext, opacity: '1'}, 1500); | ||
| + | $j('.slider-1 .flex-active-slide').find('.links').css({ bottom: bottomlinks}); | ||
| + | $j('.slider-1 .flex-active-slide').find('.links').animate({ left: '0', opacity: '1'}, 1800); | ||
| + | } | ||
| + | }); | ||
| + | |||
| + | $j('.flexslider4').flexslider({ | ||
| + | animation: 'fade', | ||
| + | animationLoop: true, //Boolean: Should the animation loop? If false, directionNav will received "disable" classes at either end | ||
| + | slideshow: true, //Boolean: Animate slider automatically | ||
| + | slideshowSpeed: 6000, //Integer: Set the speed of the slideshow cycling, in milliseconds | ||
| + | animationSpeed: 800, //Boolean: Pause the slideshow when interacting with control elements, highly recommended. | ||
| + | pauseOnHover: true, | ||
| + | pauseOnAction:true, | ||
| + | controlNav: false, | ||
| + | directionNav: true, | ||
| + | controlsContainer: '.flex-container', | ||
| + | start: function(slider) { | ||
| + | var bottomtitle = $j('.slider-2 .flex-active-slide h2').data('bottomtitle'); | ||
| + | var bottomtext = $j('.slider-2 .flex-active-slide p').data('bottomtext'); | ||
| + | var bottomlinks = $j('.slider-2 .flex-active-slide .links').data('bottomlinks'); | ||
| + | |||
| + | $j('.slider-2 .flex-active-slide').find('h2').animate({ bottom: bottomtitle, opacity: '1'}, 1500); | ||
| + | $j('.slider-2 .flex-active-slide').find('p').animate({ bottom: bottomtext, opacity: '1'}, 2000); | ||
| + | $j('.slider-2 .flex-active-slide').find('.links').animate({ bottom: bottomlinks, opacity: '1'}, 2200); | ||
| + | |||
| + | // remove class loading after start | ||
| + | slider.removeClass('loading'); | ||
| + | }, | ||
| + | before: function(slider) { | ||
| + | $j('.slider-2 .flex-active-slide').find('h2').animate({ bottom: '-20%', opacity: '0'}, 1500); | ||
| + | $j('.slider-2 .flex-active-slide').find('p').animate({ bottom: '-50%', opacity: '0'}, 2000); | ||
| + | $j('.slider-2 .flex-active-slide').find('.links').animate({ bottom: '-60%', opacity: '0'}, 2200); | ||
| + | }, | ||
| + | after: function(slider) { | ||
| + | var bottomtitle = $j('.slider-2 .flex-active-slide h2').data('bottomtitle'); | ||
| + | var bottomtext = $j('.slider-2 .flex-active-slide p').data('bottomtext'); | ||
| + | var bottomlinks = $j('.slider-2 .flex-active-slide .links').data('bottomlinks'); | ||
| + | |||
| + | $j('.slider-2 .flex-active-slide').find('h2').animate({ bottom: bottomtitle, opacity: '1'}, 1500); | ||
| + | $j('.slider-2 .flex-active-slide').find('p').animate({ bottom: bottomtext, opacity: '1'}, 2000); | ||
| + | $j('.slider-2 .flex-active-slide').find('.links').animate({ bottom: bottomlinks, opacity: '1'}, 2200); | ||
| + | } | ||
| + | }); | ||
| + | |||
| + | $j('.flexslider2').flexslider({ | ||
| + | animation: 'slide', | ||
| + | animationLoop: true, //Boolean: Should the animation loop? If false, directionNav will received "disable" classes at either end | ||
| + | slideshow: true, //Boolean: Animate slider automatically | ||
| + | slideshowSpeed: 4500, //Integer: Set the speed of the slideshow cycling, in milliseconds | ||
| + | animationSpeed: 700, //Boolean: Pause the slideshow when interacting with control elements, highly recommended. | ||
| + | pauseOnHover: true, | ||
| + | pauseOnAction:false, | ||
| + | controlNav: false, | ||
| + | directionNav: true, | ||
| + | controlsContainer: '.flex-container' | ||
| + | }); | ||
| + | |||
| + | $j('.flexslider3').flexslider({ | ||
| + | animation: 'slide', | ||
| + | animationLoop: true, //Boolean: Should the animation loop? If false, directionNav will received "disable" classes at either end | ||
| + | slideshow: false, //Boolean: Animate slider automatically | ||
| + | slideshowSpeed: 4500, //Integer: Set the speed of the slideshow cycling, in milliseconds | ||
| + | animationSpeed: 700, //Boolean: Pause the slideshow when interacting with control elements, highly recommended. | ||
| + | pauseOnHover: true, | ||
| + | pauseOnAction:false, | ||
| + | controlNav: false, | ||
| + | directionNav: true, | ||
| + | controlsContainer: '.flex-container' | ||
| + | }); | ||
| + | |||
| + | |||
| + | }); | ||
| + | |||
| + | |||
| + | |||
| + | }); | ||
| + | </script> | ||
| + | |||
</body> | </body> | ||
| + | |||
</html> | </html> | ||
Revision as of 06:27, 11 August 2017
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Continued worldwide industrialization has caused extensive environmental and human health problems. A wide variety of chemicals, e.g., heavy metals, pesticides, chlorinated solvents, etc., have been detected in different natural resources such as soil, water, and air. Among the pollutants, the heavy metals are of concern to human health due to their cytotoxicity,mutagenicity, and carcinogenicity.
Soil polluted by heavy metals represent an important environmental problem due to the toxic effects of metals, their accumulations throughout the food chain and the additional risk of groundwater contamination.
Metal remediation through common physico-chemical techniques is expensive and unsuitable in the case of extensive areas. Therefore biotechnological approaches have received a great deal of attention in recent years. Bioremediaton, as a way of natural attenuation, adds phytoremediation, the use of plants for reclamation and microbe(PSB) together as a strategy for either enhancing uptake(PSB-assisted Phytoextraction) or reducing the bioavailability of metal contaminants(PSB-immobilized Phyto-promotion mechanism) in the soil, especially in the rhizosphere to accelerate plant biomass production and root enlongation.overvie
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Phytoextraction is based on the use of hyperaccumulator plants, which can tolerate and accumulate high concentration of metals. Ideal hyperaccumulators require the characteristics of deep rooted, rapid growth and a high amount of biomass. In fact, many hyperaccumulators are slow in growth, produce low bow biomass and cannot grow in metal-stress soil. Further, the overproduction of ethylene in response to metal-stress leads to inhibition of root growth, root hair formation and consequently development of the plant as a whole. Therefore, in our project we took advantage of co-expression of ACC deaminase and iaaM genes in our chassis PSB(B. megaterium) to enhance plant growth and biomass by P solubilization and uptake particularly under stress condition by heavy metals. Meanwhile, we also exploited bacterial chemotaxis, McfS, a multiple chemoreceptor from strain P. putida F1 to detect response of organic acids secreting in the rhizosphere.
PSB-assisted Phytoextraction(ex situ)
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In this module, our team designed a pathway that the Pb ions will bind to PbR protein when activated ions are in the soil environment. It induces downstream gene expression, and results in the tetR protein inhibition promoter PltetO1 expression. At the same time, the strong promoter P43 of B.megaterium stimulated the expression of MBP in the downstream lead-producing peptide. It makes the chelating peptide of Pb metal, which reduced the total amount of heavy metals in the soil to be harmless level. When there is no Pb ions presented in the environment, the expression of tetR was blocked and the inhibition of PltetO1 was released. It results in the lacI repressor binding to the lacO sequence, and leads to blocking the expression of MBP. This mechanism is effective in preventing the infinite expression of MBP against the B. megaterium itself.
PSB-immobilized Phyto-promotion
