Parts

Name	Type	Description	Designer	Length
BBa_K2384000	DNA	T7 RNA polymerase	Yi Cai	2649
BBa_K2384001	DNA	Lpp-OmpA-MBP(Pb)	Yi Cai	939
BBa_K2384002	DNA	Lpp-OmpA-MBP(Hg)	Size Cai	939
BBa_K2384003	DNA	DsbA+MBP(Pb)	Size Cai	963
BBa_K2384004	DNA	DsbA+MBP(Hg)	Size Cai	963
BBa_K2384005	DNA	ACC deaminase	Size Cai	469
BBa_K2384006	Composite	Pveg+RBS+CspC+6*HIS+T1 terminator+T7 terminator	Haiyang Chen	469
BBa_K2384007	DNA	GST-CRS5	Size Cai	932
BBa_K2384008	Signalling	McpB	Size Cai	1986
BBa_K2384009	Regulatory	TetR	Size Cai	847
BBa_K2384010	Regulatory	LacI	Size Cai	1427
BBa_K2384011	DNA	mf-Lon	Size Cai	2619
BBa_K2384013	Composite	Repressor gene xylR+Strong PxylA promoter+RBS+GST-CRS5+His-tag	Fangfei Niu	2467
BBa_K2384014	Composite	Safeguard-toggle switch	Fangfei Niu	5004
BBa_K2384015	Composite	Repressor gene xylR+Strong PxylA promoter+RBS+Lpp-Omp-MBP(Pb)+His-tag	Fangfei Niu	1912
BBa_K2384017	DNA	CspC(For Bacillus megaterium)	Haiyang Chen	210
BBa_K2384018	Composite	Repressor gene xylR+Strong PxylA promoter+RBS+DsbA-MBP(Pb)+His-tag	Haiyang Chen	2420

Improvement

Pveg+RBS+CspC+T1 terminator+T7 terminator

This is an improve part.This gene is a cold resistant gene from the Arctic cryophile Polaribacter irgensii.It can largely increase the cold resistance of bacterias.CDS sequence optimization in (BBa_K538004) is better suited for expression in Bacillus megaterium and we add a 6*His tag at the C end.After that, the promoter (BBa_K143012) and RBS (BBa_K090505) were combined.

Optimization Report

1.Codon Used Adjustment The best value is 1 for sequence optimization.