Difference between revisions of "Team:NWU-CHINA/InterLab"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<h1>Interlab Study</h1>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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        <h3>Overview</h3>
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          <p>As GFP is an useful indicator, we are delighted to participate in the Interlab Measurement Study to help HQ researching the differences of results from all participated IGEM team all over the world.</p>
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          <p>This year, we measured 6 devices and two controls. There are totally 3/5 promoters, which were combined differently in different device. Therefore, we can measure different result caused by different combination. The three promoters, namely J23101, J23106, J23117, were cloned into pSB1C3  vector and submitted by Igem Team Berkley in 2006. The two controls, I20270 and R0040, were used as reference device.</p>
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        <h3>Experiment </h3>
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          <p>Firstly, We test our competent cells as HQ requirement. We followed protocol on the competent cells test page. Their competent met requirement of competent cells. </p>
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<h1>InterLab</h1>
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    <img src="#"/>01
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<h3>Bronze Medal Criterion #4</h3>
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  <p> Detail data is shown as follows: </p>
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<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or improve the characterization of an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2017 part number range.
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  <p>Then we transferred devices into our competent cells. At the beginning, because our chlor lost efficiency, our cell couldn’t be selected. </p>
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For teams participating in the <a href="https://2017.igem.org/Competition/InterLab_Study">InterLab study</a>, all work must be shown on this page.
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<img src="#"/>03
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  <p>After changing antibiotic, we conducted our Interlab Study fomally.</p>
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<h3>Results</h3>
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  <p>We did this experiment twice. For the first time, we didn’t get ideal data. Our cells’ fluorescence reading didn’t increase with time passing. It means that our cell didn’t express the device.</p>
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  <p>For the second time, we got data relevant with time. However, our reading value is really low. We used agarose gel electrophoresis to detect our gene expression condition.</p>
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  <p>As the picture shows, every device have transferred into chassis successfully. Therefore, we think that the reason our reading value low is because of our measuring setting. Our gain setting is 35, which leads to a low reading value. We also agree that this setting influence results a lot. We found that blank well of plate also have reading. Our low reading setting may let these blank well reading influence our result.</p>
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  <p>Our first experiment result is not good enough, so we won’t show it off. Our second experiment results are as follows: </p>
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  <br>Although the fluorescence reading is low, it still shows a obvious relevance with time.
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  <img src="#">06
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  <img src="#">07
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  <img src="#">08
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  <p> During our experiment, we found that device 1, device 3, device 6 didn’t express well. However, when putting cells under UV, Device 1 shows obvious fluorescence, while device 3 and device 6 still have no phenomenon. </p>
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  <img src="#">09
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  <br>Our Abs reading and cell culture results are as follows:
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  <img src="#">11
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  <img src="#">12
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  <img src="#">13
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Revision as of 09:06, 14 October 2017

Interlab Study

Overview

As GFP is an useful indicator, we are delighted to participate in the Interlab Measurement Study to help HQ researching the differences of results from all participated IGEM team all over the world.

This year, we measured 6 devices and two controls. There are totally 3/5 promoters, which were combined differently in different device. Therefore, we can measure different result caused by different combination. The three promoters, namely J23101, J23106, J23117, were cloned into pSB1C3 vector and submitted by Igem Team Berkley in 2006. The two controls, I20270 and R0040, were used as reference device.

Experiment

Firstly, We test our competent cells as HQ requirement. We followed protocol on the competent cells test page. Their competent met requirement of competent cells.

01

Detail data is shown as follows:

02

Then we transferred devices into our competent cells. At the beginning, because our chlor lost efficiency, our cell couldn’t be selected.

03

After changing antibiotic, we conducted our Interlab Study fomally.

Results

We did this experiment twice. For the first time, we didn’t get ideal data. Our cells’ fluorescence reading didn’t increase with time passing. It means that our cell didn’t express the device.

04

For the second time, we got data relevant with time. However, our reading value is really low. We used agarose gel electrophoresis to detect our gene expression condition.

05

As the picture shows, every device have transferred into chassis successfully. Therefore, we think that the reason our reading value low is because of our measuring setting. Our gain setting is 35, which leads to a low reading value. We also agree that this setting influence results a lot. We found that blank well of plate also have reading. Our low reading setting may let these blank well reading influence our result.

Our first experiment result is not good enough, so we won’t show it off. Our second experiment results are as follows:


Although the fluorescence reading is low, it still shows a obvious relevance with time. 06 07 08

During our experiment, we found that device 1, device 3, device 6 didn’t express well. However, when putting cells under UV, Device 1 shows obvious fluorescence, while device 3 and device 6 still have no phenomenon.

09 10
Our Abs reading and cell culture results are as follows: 11 12 13