Difference between revisions of "Team:Stony Brook/Notebook/October"

Weeks 13-17

8/21:

    • Prep for testing

8/22: MRSA Testing day 1

    • Hydrate MRSA and Staphylococcus Aureus

    • Create liquid cultures in preparation for testing

    • Gel Extraction of two samples each of lnqZE, lnqZA, lnqZQ, lnq Z

8/23: MRSA testing day 2

    • Performed zone of inhibition test

8/24: MRSA testing day 3

    • Analyzed results of the zone of inhibition test

    • Performed test to see if any protein would kill a liquid culture of the cells

    • Gel Extraction of two samples each of Z+E, Z+A, ZQ, Lnq Z

8/25:

    • Analyzed the results

    • Killed all MRSA and Staphylococcus aureus samples

    • Disinfected lab

9/01:

    • Transform DH5ɑ cells with iGEM plasmids and plate.

9/02:

    • Make small liquid cultures of both plasmid 1 and plasmid 2

9/03:

    • MiniPrep iGEM plasmids

    • RE Digest of the iGEM plasmids

9/05:

    • Ran digests on gel and gel extracted

    • Make small liquid cultures of GST Epi, GST A53, GST Lnq Z, MBP Z+A, and MBP Z+Q

9/06:

    • Nanodrop of gel extract

    • Miniprep small liquid cultures of constructs

9/11:

    • BioBrick MiniPrep Nanodrop

10/13:

    • Gel

10/18:

    • Restriction Enzyme Digest of Aureocin A53 and Epi

    • Prep BioBricks

10/22:

    • Dot Blot

10/23:

    • Dot Blot