|
|
| Line 5: |
Line 5: |
| | <!-- The content of this page --> | | <!-- The content of this page --> |
| | <div class="container"> | | <div class="container"> |
| | + | <div id="title" style="background-image: url(https://static.igem.org/mediawiki/2017/a/ae/T--Bielefeld-CeBiTec--title-img-cebitec.jpg);"> |
| | + | <img src="https://static.igem.org/mediawiki/2017/a/ae/T--Bielefeld-CeBiTec--title-img-cebitec.jpg"> |
| | + | <div id="title-bg"> |
| | + | <div id="title-text"> |
| | + | # |
| | + | </div> |
| | + | </div> |
| | + | </div> |
| | <div class="contentbox"> | | <div class="contentbox"> |
| | <div class="bevel tr"></div> | | <div class="bevel tr"></div> |
| | <div class="content"> | | <div class="content"> |
| − | <h2> Main Title </h2>
| |
| | <h4> Subtitle 1 </h4> | | <h4> Subtitle 1 </h4> |
| | <p class="test"> Test </p> | | <p class="test"> Test </p> |
| Line 14: |
Line 21: |
| | Lorem ipsum dolor sit amet, <a href="#link-jump">consetetur sadipscing</a> elitr, sed diam nonumy <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Notebook/Methods">eirmod tempor</a> invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. | | Lorem ipsum dolor sit amet, <a href="#link-jump">consetetur sadipscing</a> elitr, sed diam nonumy <a href="https://2017.igem.org/Team:Bielefeld-CeBiTec/Notebook/Methods">eirmod tempor</a> invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. |
| | </article> | | </article> |
| | + | <object data="https://static.igem.org/mediawiki/2017/9/9a/T--Bielefeld-CeBiTec--hp-overview-code-sun.svg" type="image/svg+xml"> |
| | + | <!---Fallback---> |
| | + | Browser cannot display svg images! |
| | + | </object> |
| | + | <table class=" pgrouptable tablesorter" style="width: 100%;" cellspacing="0" cellpadding="0"> |
| | + | <thead> |
| | + | <tr><th style="width:8px" class="header headerSortDown"></th><th style="width:8px" class="header"></th><th style="width:8px" class="header"></th><th style="width: 87px;" class="header">Name</th><th style="width: 80px" colspan="" class="header">Type</th><th style="width: auto;" colspan="" class="header">Description</th><th style="width: 150px" colspan="" class="header">Designer</th><th style="width: 50px" colspan="" class="header">Length</th> |
| | + | </tr></thead><tbody> |
| | + | <tr> |
| | + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2201200">BBa_K2201200</a></td><td>Coding</td><td>tRNA synthetase/tRNA needed for incorporating 2-nitrophenylalanine </td><td width="100px">Yannic Kerkhoff</td><td align="right">1738</td> |
| | + | </tr><tr> |
| | + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2201220">BBa_K2201220</a></td><td>Coding</td><td>CDS of GFP-streptavidin fusion protein with medium gly-gly-ser linker</td><td width="100px">Yannic Kerkhoff</td><td align="right">1131</td> |
| | + | </tr><tr> |
| | + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2201221">BBa_K2201221</a></td><td>Coding</td><td>CDS of GFP-streptavidin fusion protein with medium gly-gly-ser linker containing an amber-codon</td><td width="100px">Yannic Kerkhoff</td><td align="right">1131</td> |
| | + | </tr><tr> |
| | + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2201320">BBa_K2201320</a></td><td>Composite</td><td>functional GFP-streptavidin fusion protein with medium gly-gly-ser linker and T7-promoter and RBS</td><td width="100px">Yannic Kerkhoff</td><td align="right">1169</td> |
| | + | </tr><tr> |
| | + | <td class="status_cell "></td><td class="status_cell cell_white"> </td><td class="status_cell cell_white"> </td><td><a class="noul_link part_link" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2201321">BBa_K2201321</a></td><td>Composite</td><td>functional GFP-streptavidin fusion protein with medium gly-gly-ser linker containing an amber codon </td><td width="100px">Yannic Kerkhoff</td><td align="right">1169</td> |
| | + | </tr></tbody> |
| | + | </table> |
| | + | <div class="contentline"> |
| | + | <div class="half left"> |
| | + | <article> |
| | + | CL is an amino acid consisting of cysteine and lysine. The cysteine was coupled to the side |
| | + | chain of lysine so that CL contains a free 1,2-aminothiol group |
| | + | (Nguyen <i>et al.</i>, 2011). This is an important characteristic for the |
| | + | specific binding between the CL and the CBT‑Asp. |
| | + | <ul> |
| | + | <li>Name: N<sup>ε</sup>-L-cysteinyl-L-lysine</li> |
| | + | <li>Short: CL |
| | + | <li>Molecular Weight: 249.33 g mol<sup>-1</sup></li> |
| | + | <li>Storage: -20 – 4 °C</li> |
| | + | <li>Function: Terminus independent binding system |
| | + | </ul> |
| | + | </article> |
| | + | |
| | + | </div> |
| | + | <div class="half right"> |
| | + | <div class="figure large"> |
| | + | <img class="figure image" src="https://static.igem.org/mediawiki/2017/2/22/T--Bielefeld-CeBiTec--27-08-17-CL_structure.png"> |
| | + | <p class="figure subtitle"> |
| | + | <b>Figure 3: Structure of CL.</b> |
| | + | </p> |
| | + | </div> |
| | + | </div> |
| | + | </div> |
| | + | <div class="figure medium"> |
| | + | <img class="figure image" src="expand_black_bg.svg"> |
| | + | <p class="figure subtitle"><b>Figure 6: Repression values dependent on inter‑operator distances between O1 and O<sub>id</sub>.</b><br>The repression values refer to the repression of the chromosomal <i>lacZ</i> gene under the control of O1 at its natural positon and O<sub>id</sub> at the indicated position. With 50 tetrameric Lac repressors per cell the repression value is calculated by the specific activity of β‑galactosidase in absence of active Lac repressor devided by the specific activity of β‑galactosidase in the presence of active Lac repressor. The dashed line shows the repression value for a single natural O1 operator (Mueller <i>et al</i>., 1996).</p> |
| | + | </div> |
| | + | <div class="figure medium"> |
| | + | <img class="figure image" src="https://static.igem.org/mediawiki/2017/c/c5/T--Bielefeld-CeBiTec--Oid_o1_bp_distance.png"> |
| | + | <p class="figure subtitle"><b>Figure 6: Repression values dependent on inter‑operator distances between O1 and O<sub>id</sub>.</b><br>The repression values refer to the repression of the chromosomal <i>lacZ</i> gene under the control of O1 at its natural positon and O<sub>id</sub> at the indicated position. With 50 tetrameric Lac repressors per cell the repression value is calculated by the specific activity of β‑galactosidase in absence of active Lac repressor devided by the specific activity of β‑galactosidase in the presence of active Lac repressor. The dashed line shows the repression value for a single natural O1 operator (Mueller <i>et al</i>., 1996).</p> |
| | + | </div> |
| | <div class="figure small"> | | <div class="figure small"> |
| | <img class="figure image" src="Svenjas_Zeichnung.png"> | | <img class="figure image" src="Svenjas_Zeichnung.png"> |
| Line 161: |
Line 222: |
| | Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. | | Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. |
| | </article> | | </article> |
| | + | <div class="contentline"> |
| | + | <div class="half left"> |
| | + | <article> |
| | + | CL is an amino acid consisting of cysteine and lysine. The cysteine was coupled to the side |
| | + | chain of lysine so that CL contains a free 1,2-aminothiol group |
| | + | (Nguyen <i>et al.</i>, 2011). This is an important characteristic for the |
| | + | specific binding between the CL and the CBT‑Asp. |
| | + | <ul> |
| | + | <li>Name: N<sup>ε</sup>-L-cysteinyl-L-lysine</li> |
| | + | <li>Short: CL |
| | + | <li>Molecular Weight: 249.33 g mol<sup>-1</sup></li> |
| | + | <li>Storage: -20 – 4 °C</li> |
| | + | <li>Function: Terminus independent binding system |
| | + | </ul> |
| | + | </article> |
| | + | |
| | + | </div> |
| | + | <div class="half right"> |
| | + | <div class="figure large"> |
| | + | <img class="figure image" src="https://static.igem.org/mediawiki/2017/2/22/T--Bielefeld-CeBiTec--27-08-17-CL_structure.png"> |
| | + | <p class="figure subtitle"> |
| | + | <b>Figure 3: Structure of CL.</b> |
| | + | </p> |
| | + | </div> |
| | + | </div> |
| | + | </div> |
| | </div> | | </div> |
| | <div class="bevel bl"></div> | | <div class="bevel bl"></div> |
| Line 173: |
Line 260: |
| | </div> | | </div> |
| | <div class="bevel bl"></div> | | <div class="bevel bl"></div> |
| − | </div> | + | </div> |
| | </body> | | </body> |
| | </html> | | </html> |
| | {{Team:Bielefeld-CeBiTec/Footer}} | | {{Team:Bielefeld-CeBiTec/Footer}} |
