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Revision as of 04:16, 13 October 2017

ecDNA

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PROJECT OVERVIEW



Cancer, a genetic disease resulting in uncontrollable cell growth, is mostly caused by somatic mutations acquired throughout an individual’s lifetime. Recently, it was discovered that some oncogenes resided on extrachromosomal DNA (ecDNA). Because ecDNA causes oncogene copy number to increase exponentially, utilizing CRISPR to create breaks in ecDNA decreases cancer cells’ replication speed.

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NOTEBOOK



Over several months in the lab, we've gone from concept to completion in creating an E.coli strain that synthesizes and secretes chitinase. Have an inside look at the processes and procedures that led to our success.

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