Difference between revisions of "Team:Tec-Chihuahua/timeline"

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                         <div class="col-md-6 col-md-offset-6 journal-right">
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                             <h3 class="quote-date"><b>April</b> / 28 / 2017</h3>
+
                             <h3 class="quote-date"><b>February</b> / 07 / 2017</h3>
 
                             <div class="col-md-12 in-quote">
 
                             <div class="col-md-12 in-quote">
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                                 <p>After a lot of investigation and discussion, we decided on our project for this year's competition, based on the bacteria “Erwinia amylovora”</p>     
+
                                 <p>The 2016 IGem team members made an introductory presentation for the new members.
 +
</p>     
 
                             </div>
 
                             </div>
 
                              
 
                              
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                             <h3 class="quote-date"><b>Mar</b> / 14 / 2015</h3>
+
                             <h3 class="quote-date"><b>February</b> / 14-27 / 2015</h3>
 
                             <div class="col-md-12 in-quote">
 
                             <div class="col-md-12 in-quote">
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                                 <p>We won the Entrepreneurship Contest  of the Nucleum Congress in Monterrey.</p>                               
+
                                 <p>Fundraising events: in order to pay the inscription fee.</p>                               
 
                             </div>
 
                             </div>
 
                              
 
                              
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                             <h3 class="quote-date"><b>Mar</b> / 19 / 2015</h3>
+
                             <h3 class="quote-date"><b>Mar</b> / 13 / 2017</h3>
 
                             <div class="col-md-12 in-quote">
 
                             <div class="col-md-12 in-quote">
−
                                 <p>Team Meeting.</p>     
+
                                 <p>Snapgene sponsorship confirmation.</p>     
 
                             </div>
 
                             </div>
 
                              
 
                              
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                             <h3 class="quote-date"><b>Mar</b> / 26 / 2015</h3>
+
                             <h3 class="quote-date"><b>April</b> / 01 / 2017</h3>
 
                             <div class="col-md-12 in-quote">
 
                             <div class="col-md-12 in-quote">
−
                                 <p>Team promotional photo.</p>
+
                                 <p>Tec Chihuahua is officially an iGem 2017 team.</p>
−
                                <img src="https://static.igem.org/mediawiki/2015/7/7c/Tec-chihuahua-notebook-march04.jpeg">
+
                           
 
                             </div>
 
                             </div>
 
                              
 
                              
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                             <h3 class="quote-date"><b>Abr</b> / 2 / 2015</h3>
+
                             <h3 class="quote-date"><b>April</b> / 28 / 2015</h3>
 
                             <div class="col-md-12 in-quote">
 
                             <div class="col-md-12 in-quote">
−
                                 <p>Team Meeting</p>     
+
                                 <p>After a lot of investigation and discussion, we decided on our project for this year's competition, based on the bacteria “Erwinia amylovora”</p>     
 
                             </div>
 
                             </div>
 
                              
 
                              
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                             <h3 class="quote-date"><b>Abr</b> / 9 / 2015</h3>
+
                             <h3 class="quote-date"><b>May</b> / 21 / 2017</h3>
 
                             <div class="col-md-12 in-quote">
 
                             <div class="col-md-12 in-quote">
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                                 <p>We presented our ideas to experts of the Centro de Investigación en Materiales Avanzados (CIMAV), they seem interested on the Project and gave us their support. </p>
+
                                 <p>After a lot of investigation we decided on the targets we wanted to inhibit in Erwinia Amylovora and the three genes that we will use for this purpose “aiiA, epsE and yhjH”. We also made the first draft of our biopart. </p>
−
                                <img src="https://static.igem.org/mediawiki/2015/f/fa/Tec-chihuahua-notebook-abril09.jpeg" >
+
                             
 
                             </div>
 
                             </div>
 
                              
 
                              
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                             <h3 class="quote-date"><b>Abr</b> / 17 / 2015</h3>
+
                             <h3 class="quote-date"><b>May</b> / 23 / 2017</h3>
 
                             <div class="col-md-12 in-quote">
 
                             <div class="col-md-12 in-quote">
−
                                 <p>We went to the local TV Show “Emma de la O” to talk about the biotechnology and our project.</p>     
+
                                 <p>We made the annuouncement for courses of synthetic biology and mathematical model for the new members of the team and the team supporters.</p>     
−
                                 <img src="https://static.igem.org/mediawiki/2015/d/d8/Tec-chihuahua-notebook-abril17.jpeg">
+
                                  
 
                             </div>
 
                             </div>
 
                              
 
                              
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                             <h3 class="quote-date"><b>Abr</b> / 20 / 2015</h3>
+
                             <h3 class="quote-date"><b>May</b> / 24 / 2017</h3>
 
                             <div class="col-md-12 in-quote">
 
                             <div class="col-md-12 in-quote">
−
                                 <p>Synthesis of the NP-CNT. We began the synthesis of the nanotubes at CIMAV.</p>
+
                                 <p>We started the  
 +
training on  synthetic biology with the first session: “Bacterial transformation”
 +
 
 +
The first meeting of the mathematical model and human practices.
 +
</p>
 
                                  
 
                                  
 
                             </div>
 
                             </div>
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                             <h3 class="quote-date"><b>May</b> / 12 - 15 / 2015</h3>
+
                             <h3 class="quote-date"><b>May</b> / 25 / 2017</h3>
 
                             <div class="col-md-12 in-quote">
 
                             <div class="col-md-12 in-quote">
−
                                 <p>Purification of the NP-CNT</p>     
+
                                 <p>Second day of training: “Laboratory material sterilization and preparation of culture media ”</p>     
−
                                 <img src="https://static.igem.org/mediawiki/2015/e/e4/Tec-chihuahua-notebook-may12.jpeg">
+
                                  
 
                             </div>
 
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                             <h3 class="quote-date"><b>May</b> / 20 / 2015</h3>
+
                             <h3 class="quote-date"><b>May</b> / 26 / 2017</h3>
 
                             <div class="col-md-12 in-quote">
 
                             <div class="col-md-12 in-quote">
−
                                 <p>Filtration of the P-CNT</p>
+
                                 <p>Third day of training: “General microbiology and PCR”</p>
 
                                  
 
                                  
 
                             </div>
 
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                             <h3 class="quote-date"><b>May</b> / 21 / 2015</h3>
+
                             <h3 class="quote-date"><b>May</b> / 29 / 2017</h3>
 
                             <div class="col-md-12 in-quote">
 
                             <div class="col-md-12 in-quote">
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                                 <p>Preparation of Luria Bertani Broth for <span class="bacteria">E. coli</span></p>     
+
                                 <p>Fourth day of training: “Plasmid DNA extraction”
 +
Also, was the first wet lab day!
 +
</p>     
 
                             </div>
 
                             </div>
 
                              
 
                              
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                             <h3 class="quote-date"><b>May</b> / 22 / 2015</h3>
+
                             <h3 class="quote-date"><b>May</b> / 30 / 2017</h3>
 
                             <div class="col-md-12 in-quote">
 
                             <div class="col-md-12 in-quote">
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                                 <p>We finished the filtration of the nanotubes</p>
+
                                 <p>Fifth day of training: “Ligation and digestion”</p>
−
                                <p>Plasmid extraction of <span class="bacteria">E. coli</span></p>
+
−
                                <img src="https://static.igem.org/mediawiki/2015/9/9d/Tec-chihuahua-notebook-may22.jpeg">
+
 
                                  
 
                                  
 
                             </div>
 
                             </div>
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                             <h3 class="quote-date"><b>May</b> / 25 / 2015</h3>
+
                             <h3 class="quote-date"><b>May</b> / 31 / 2017</h3>
 
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                                 <p>We measured the purity of plasmid using the NanoDrop 2000</p>     
+
                                 <p>We finished the training in synthetic biology with the session: “Electrophoresis”</p>     
−
                                <img src="https://static.igem.org/mediawiki/2015/8/8f/Tec-chihuahua-notebook-may25.jpeg">
+
                         
 
                             </div>
 
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                             <h3 class="quote-date"><b>May</b> / 27 / 2015</h3>
+
                             <h3 class="quote-date"><b>June</b> /03 / 2017</h3>
 
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                                 <p>Team meeting to discuss about the wiki</p>
 
                                 <p>Team meeting to discuss about the wiki</p>

Revision as of 23:19, 17 October 2017

Erwinions














Enero / 25 / 2017

First IGem 2017 team meeting.

February / 07 / 2017

The 2016 IGem team members made an introductory presentation for the new members.

February / 14-27 / 2015

Fundraising events: in order to pay the inscription fee.

Mar / 13 / 2017

Snapgene sponsorship confirmation.

April / 01 / 2017

Tec Chihuahua is officially an iGem 2017 team.

April / 28 / 2015

After a lot of investigation and discussion, we decided on our project for this year's competition, based on the bacteria “Erwinia amylovora”

May / 21 / 2017

After a lot of investigation we decided on the targets we wanted to inhibit in Erwinia Amylovora and the three genes that we will use for this purpose “aiiA, epsE and yhjH”. We also made the first draft of our biopart.

May / 23 / 2017

We made the annuouncement for courses of synthetic biology and mathematical model for the new members of the team and the team supporters.

May / 24 / 2017

We started the training on synthetic biology with the first session: “Bacterial transformation” The first meeting of the mathematical model and human practices.

May / 25 / 2017

Second day of training: “Laboratory material sterilization and preparation of culture media ”

May / 26 / 2017

Third day of training: “General microbiology and PCR”

May / 29 / 2017

Fourth day of training: “Plasmid DNA extraction” Also, was the first wet lab day!

May / 30 / 2017

Fifth day of training: “Ligation and digestion”

May / 31 / 2017

We finished the training in synthetic biology with the session: “Electrophoresis”

June /03 / 2017

Team meeting to discuss about the wiki

May / 28 / 2015

Inventory of team´s material and lab cleanup.

Jun / 1 / 2015

Preparation of Luria Bertani agar.

Team meeting for fund collection.

Sterilizing of work solutions.

We began to dry the P-CNT

Jun / 3 / 2015

We finished drying the nanotubes.

Preparation of PBS

Jun / 8 / 2015

We began the work with the bovine oocytes at the Facultad de Zootecnia (School of Animal Husbandry) of the Universidad Autónoma de Chihuahua (UACH).

Jun / 9 / 2015

Arriving of the Florescein Diacetate.

Oocytes extraction and selection.

The maduration of the viables oocytes began.

Jun / 10 / 2015

We began the CNT-Fluorescein functionalization.

Cleaning and picking of the oocytes.

Jun / 11 / 2015

We finished the CNT functionalization with fluorescein.

Jun / 15 / 2015

Pins Ceremony

Jun / 16 / 2015

F-NTC / Oocytes interaction. We failed

Jun / 18 / 2015

Purification of the Second batch of CNT.

Jun / 22 / 2015

Preparation of the stocks and the media for the handling and maduration of oocytes.

Jun / 24 / 2015

Oocytes extraction and selection.

The maduration of the viables oocytes began.

Jun/ 25 / 2015

Cleaning and picking of the oocytes.

F-NTC / Oocytes interaction.

Filtration of the second group of nanotubes

Jun / 26 / 2015

“About our lab” and “About our proyect” Questionnaires.

Jun / 29 / 2015

Team meeting.

Observation of functionalized oocytes with confocal microscope.

Jun / 30 / 2015

Filtration of the CNT.

Jul / 1 / 2015

We finished the filtration of the second batch of CNT.

Jul / 2 / 2015

Drying of the P-CNT.

Oocytes extraction and selection.

Preparation of work solutions.

Jul / 3 / 2015

Preparation of work solutions.

Jul / 4 / 2015

F-NTC / Oocytes interaction and staining of the oocytes with DAPI.

Jul / 6 /2015

Team meeting

Jul / 7 / 2015

Presentation to high school students about Synthetic Biology for the Human Practices.

Jul / 8 / 2015

Oocytes extraction and selection for maduration

We fabricated glass pipettes

Wiki meeting with Sammy.

Jul / 9 / 2015

Cleaning and picking of the oocytes.

Jul / 10 / 2015

F-NTC / Oocytes interaction and staining of the oocytes with DAPI.

Jul / 13 / 2015

We took a Biosafety Course for the Safety requirements.

Wiki meeting with Sammy

Jul / 15 / 2015

Team meeting.

Jul / 16 / 2015

Wiki meeting with Sammy.

Jul / 17 / 2015

Preparation of work solutions.

Jul / 20 / 2015

Meeting with Dr. Quintín Rascon to planning the work on N. tabacum.

Jul / 21 / 2015

We started Functionalization with Fluorescein of the second batch of P-CNT.

Oocytes extraction and selection for maduration.

Jul / 22 / 2015

We finished the CNT-functionalization.

Jul / 23 / 2015

Preparation of solutions.

Interaction of the oocytes with the F-CNT (both of the batches), previously fixed with paraformaldehyde.

Jul / 24 / 2015

Team meeting

Jul / 27 - 31 / 2015

Research and documentary work.

Working on the wiki content.

Aug / 03 / 2015

Wiki Content work

Team meating

Aug / 04 - 05 / 2015

Wiki meeting with Sammy

Aug / 06 / 2015

we grew E. coli TOP10 on LB brith and incubated it on shaker

Aug / 07 / 2015

Sterilizing solutions

Aliquots of E. coli and glycerol were prepared

Aug / 10 / 2015

Team meeting

Aug / 11 / 2015

Preparation of Solutions

Preparation of LB Broth

The optical density of TOP10 was measured

Aug / 12 - 14 / 2015

Biobricks cloning started: We failed on cell transformation

Sep / 04 - 18 / 2015

Lab work with the biobricks

Fast and Furiously Finishing the Wiki

Last details of presentation and poster

Making of promotional video.


Months of work finally come to end!