Difference between revisions of "Team:TP-CC San Diego/InterLab"

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Similar to chromosomal DNA, ecDNA is composed by double strands of nucleic acid but form a circular structure. More importantly, ecDNA does not have a centromere for spindle fiber binding during mitosis. This unique feature allows rapid DNA multiplication  and random segregation to create high heterogeneity in daughter cells during cell proliferation, implying a possible correlative relationship between the development of tumors and a faster resistance to existing treatments.
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<h3 class = "subtitle">Data</h3>
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   <img src="https://static.igem.org/mediawiki/2017/7/77/T--TP-CC_San_Diego--Fluorescence_Raw_Readings.png" width="450">
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In 1965, extrachromosomal DNA (ecDNA) was discovered; DNA free from its traditional homes in the nucleus was documented. One study taking a look at ecDNA by means of fluorescence in situ hybridization proposed the ecDNA’s unusual number of oncogenes, but it didn’t catch enough attention because it was considered to be a rare event. Not until recently has the importance of ecDNA been revisited. The most recent study revealed that nearly 40% of oncogenes reside on ecDNA rather than the widely accepted notion that all DNA resided only on chromosomes.
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   <img src="https://static.igem.org/mediawiki/2017/9/9e/T--TP-CC_San_Diego--Abs600_Raw_Readings.png" width="450">
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   <img src="https://static.igem.org/mediawiki/2017/f/f8/T--TP-CC_San_Diego--platepattern.png" width="450">
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<h3 class = "subtitle">Results</h3>
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   <img class="cardleft" src="https://static.igem.org/mediawiki/2017/7/77/T--TP-CC_San_Diego--Fluorescence_Raw_Readings.png" width="450">
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   <img src="https://static.igem.org/mediawiki/2017/3/3e/T--TP-CC_San_Diego--Unit_Scaling_Factors.png" width="450">
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Similar to chromosomal DNA, ecDNA is composed by double strands of nucleic acid but form a circular structure. More importantly, ecDNA does not have a centromere for spindle fiber binding during mitosis. This unique feature allows rapid DNA multiplication  and random segregation to create high heterogeneity in daughter cells during cell proliferation, implying a possible correlative relationship between the development of tumors and a faster resistance to existing treatments.
+
   <img src="https://static.igem.org/mediawiki/2017/f/f6/T--TP-CC_San_Diego--Experimental_Values-_Raw_Abs600.png" width="450">
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   </p>
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   <img src="https://static.igem.org/mediawiki/2017/6/61/T--TP-CC_San_Diego--Experimental_Values-_Raw_Fluorescence.png" width="450">
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<div class="img-row">
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<h3 class = "subtitle">Data</h3>
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   <img src="https://static.igem.org/mediawiki/2017/e/ea/T--TP-CC_San_Diego--Experimental_Values-_OD_-_Background.png" width="450">
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  <p class="cardleft">
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In 1965, extrachromosomal DNA (ecDNA) was discovered; DNA free from its traditional homes in the nucleus was documented. One study taking a look at ecDNA by means of fluorescence in situ hybridization proposed the ecDNA’s unusual number of oncogenes, but it didn’t catch enough attention because it was considered to be a rare event. Not until recently has the importance of ecDNA been revisited. The most recent study revealed that nearly 40% of oncogenes reside on ecDNA rather than the widely accepted notion that all DNA resided only on chromosomes.
+
   <img src="https://static.igem.org/mediawiki/2017/e/ee/T--TP-CC_San_Diego--Experimental_Values-_Fluorescence_-_Background_-_Background.png" width="450">
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  </p>
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  <img class="cardright" src="https://static.igem.org/mediawiki/2017/9/9e/T--TP-CC_San_Diego--Abs600_Raw_Readings.png" width="450">
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   <img src="https://static.igem.org/mediawiki/2017/e/ee/OD600.png" width="450">
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</div>
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<div class="img-row">
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   <img src="https://static.igem.org/mediawiki/2017/3/3b/T--TP-CC_San_Diego--Experimental_Values-_Experimental_Values--Summary_Statistics.png">
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<h3 class = "subtitle">Results</h3>
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   <img class="cardleft" src="https://static.igem.org/mediawiki/2017/f/f8/T--TP-CC_San_Diego--platepattern.png" width="450">
+
−
<p class="cardright">
+
−
Similar to chromosomal DNA, ecDNA is composed by double strands of nucleic acid but form a circular structure. More importantly, ecDNA does not have a centromere for spindle fiber binding during mitosis. This unique feature allows rapid DNA multiplication  and random segregation to create high heterogeneity in daughter cells during cell proliferation, implying a possible correlative relationship between the development of tumors and a faster resistance to existing treatments.
+
−
  </p>
+
−
</div>
+
−
<h3 class = "subtitle">Data</h3>
+
−
  <p class="cardleft">
+
−
In 1965, extrachromosomal DNA (ecDNA) was discovered; DNA free from its traditional homes in the nucleus was documented. One study taking a look at ecDNA by means of fluorescence in situ hybridization proposed the ecDNA’s unusual number of oncogenes, but it didn’t catch enough attention because it was considered to be a rare event. Not until recently has the importance of ecDNA been revisited. The most recent study revealed that nearly 40% of oncogenes reside on ecDNA rather than the widely accepted notion that all DNA resided only on chromosomes.
+
−
  </p>
+
−
   <img class="cardright" src="https://static.igem.org/mediawiki/2017/3/3e/T--TP-CC_San_Diego--Unit_Scaling_Factors.png" width="450">
+
−
</div>
+
−
<div class="img-row">
+
−
<h3 class = "subtitle">Results</h3>
+
−
   <img class="cardleft" src="https://static.igem.org/mediawiki/2017/f/f6/T--TP-CC_San_Diego--Experimental_Values-_Raw_Abs600.png" width="450">
+
−
<p class="cardright">
+
−
Similar to chromosomal DNA, ecDNA is composed by double strands of nucleic acid but form a circular structure. More importantly, ecDNA does not have a centromere for spindle fiber binding during mitosis. This unique feature allows rapid DNA multiplication  and random segregation to create high heterogeneity in daughter cells during cell proliferation, implying a possible correlative relationship between the development of tumors and a faster resistance to existing treatments.
+
−
  </p>
+
−
</div>
+
−
<h3 class = "subtitle">Data</h3>
+
−
  <p class="cardleft">
+
−
In 1965, extrachromosomal DNA (ecDNA) was discovered; DNA free from its traditional homes in the nucleus was documented. One study taking a look at ecDNA by means of fluorescence in situ hybridization proposed the ecDNA’s unusual number of oncogenes, but it didn’t catch enough attention because it was considered to be a rare event. Not until recently has the importance of ecDNA been revisited. The most recent study revealed that nearly 40% of oncogenes reside on ecDNA rather than the widely accepted notion that all DNA resided only on chromosomes.
+
−
  </p>
+
−
   <img class="cardright" src="https://static.igem.org/mediawiki/2017/6/61/T--TP-CC_San_Diego--Experimental_Values-_Raw_Fluorescence.png" width="450">
+
−
</div>
+
−
<h3 class = "subtitle">Results</h3>
+
−
   <img class="cardleft" src="https://static.igem.org/mediawiki/2017/e/ea/T--TP-CC_San_Diego--Experimental_Values-_OD_-_Background.png" width="450">
+
−
<p class="cardright">
+
−
Similar to chromosomal DNA, ecDNA is composed by double strands of nucleic acid but form a circular structure. More importantly, ecDNA does not have a centromere for spindle fiber binding during mitosis. This unique feature allows rapid DNA multiplication  and random segregation to create high heterogeneity in daughter cells during cell proliferation, implying a possible correlative relationship between the development of tumors and a faster resistance to existing treatments.
+
−
  </p>
+
−
</div>
+
−
<h3 class = "subtitle">Data</h3>
+
−
  <p class="cardleft">
+
−
In 1965, extrachromosomal DNA (ecDNA) was discovered; DNA free from its traditional homes in the nucleus was documented. One study taking a look at ecDNA by means of fluorescence in situ hybridization proposed the ecDNA’s unusual number of oncogenes, but it didn’t catch enough attention because it was considered to be a rare event. Not until recently has the importance of ecDNA been revisited. The most recent study revealed that nearly 40% of oncogenes reside on ecDNA rather than the widely accepted notion that all DNA resided only on chromosomes.
+
−
  </p>
+
−
   <img class="cardright" src="https://static.igem.org/mediawiki/2017/e/ee/T--TP-CC_San_Diego--Experimental_Values-_Fluorescence_-_Background_-_Background.png" width="450">
+
−
</div>
+
−
<h3 class = "subtitle">Results</h3>
+
−
   <img class="cardleft" src="https://static.igem.org/mediawiki/2017/e/ee/OD600.png" width="450">
+
−
<p class="cardright">
+
−
Similar to chromosomal DNA, ecDNA is composed by double strands of nucleic acid but form a circular structure. More importantly, ecDNA does not have a centromere for spindle fiber binding during mitosis. This unique feature allows rapid DNA multiplication  and random segregation to create high heterogeneity in daughter cells during cell proliferation, implying a possible correlative relationship between the development of tumors and a faster resistance to existing treatments.
+
−
  </p>
+
−
</div>
+
−
<h3 class = "subtitle">Data</h3>
+
−
  <p class="cardleft">
+
−
In 1965, extrachromosomal DNA (ecDNA) was discovered; DNA free from its traditional homes in the nucleus was documented. One study taking a look at ecDNA by means of fluorescence in situ hybridization proposed the ecDNA’s unusual number of oncogenes, but it didn’t catch enough attention because it was considered to be a rare event. Not until recently has the importance of ecDNA been revisited. The most recent study revealed that nearly 40% of oncogenes reside on ecDNA rather than the widely accepted notion that all DNA resided only on chromosomes.
+
−
  </p>
+
−
   <img class="cardright" src="https://static.igem.org/mediawiki/2017/3/3b/T--TP-CC_San_Diego--Experimental_Values-_Experimental_Values--Summary_Statistics.png">
+
 
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Revision as of 04:56, 22 October 2017


InterLab

Interlab

Introduction

This year’s interlab study is intended to answer the main question of the relativity of fluorescence when measured at different parts of the world. To make this data more reliable, iGem has asked to test some RBS devices to make gene expression reliable and precise. For this year’s interlab study, TP-CC San Diego has decided to do the plate reader protocol along with transforming the plasmids from Kit 7.

Method

Data

Results