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Revision as of 19:34, 24 October 2017
Notebook
On this page, Gaston Day School iGEM team would like to share with you our notes during the experiments from 09/01/2017 to 10/23/2017. The team leader Heena Saqib records the data patiently.
MeCl:
| ??? | ??? | ??? |
|---|---|---|
| 14.3 | H2O | 114.4 |
| 2.5 | Buffer | 20 |
| 1 | MgCl2 | 8 |
| 1 | dNTP | 8 |
tube:
| 2ul | H2O |
| -5ul | F |
| -5ul | R |
Lable:
| 0 | Control |
| 1 | AceF |
| 2 | EutG BB |
| 3 | YiaY BB |
| 4 | Adhp BB |
| 5 | Lpol |
| 6 | AceE |
Maeten Mix
| gDNA | 3 | 24 |
| Buffer | 2.5 | 20 |
| ??? | 1 | 8 |
| dH2O | 13.3 | 106.4 |
| ??? | 0.2 | 1.6 |
| / | dH2O | For | Rev | / |
|---|---|---|---|---|
| 1 | 4 | 0.5 | 0.5 | AceF |
| 2 | 4 | 0.5 | 0.5 | EutG |
| 3 | 4 | 0.5 | 0.5 | YiaY |
| 4 | 4 | 0.5 | 0.5 | AdnP |
| 5 | 4 | 0.5 | 0.5 | Lpd |
| 6 | 4 | 0.5 | 0.5 | AceE |
| 7 | 5 | - | - | - |
Maeten Mix
| gDNA | 3 | 24 |
| Buffer | 2.5 | 20 |
| dNTP | 1 | 8 |
| dH2O | 12.3 | 98.4 |
| taq | 0.2 | 1.6 |
| MgCl2 | 1 | 8 |
| / | dH2O | For | Rev | / |
|---|---|---|---|---|
| 0 | 5 | - | - | - |
| 1 | 4 | 0.5 | 0.5 | ACE F |
| 2 | 4 | 0.5 | 0.5 | EutG |
| 3 | 4 | 0.5 | 0.5 | YiaY |
| 4 | 4 | 0.5 | 0.5 | AdhP |
| 5 | 4 | 0.5 | 0.5 | Lpd |
| 6 | 4 | 0.5 | 0.5 | AceE |
