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With their broad chemical and functional diversity ncAAs provide a variety of promising applications including proteinlabeling, photocaging, structure analysis and specific protein interactions. Therefore, the development of a new iGEM toolkit for the translational incorporation of non-canonical amino acids (ncAAs) in E. coli would be a great contribution to the iGEM community. The site-specific incorporation of ncAAs requires the directed evolution of tRNA and aminoacyl tRNA synthetase pairs, which in turn mediate the introduction of a ncAA for a certain codon. We are especially exploring the application of unnatural base pairs (UBPs) as an expansion of the genetic code. This approach promises an optimal orthogonality to the autologous translation apparatus and a high flexibility concerning the incorporation of multiple ncAAs. We will utilize several systems to achieve a high retention efficiency. As examples for the successful incorporation of non-canonical amino acids we will develop a rapid test for prions and a new chromatography method for a mild elution of proteins.

@@ Line 3: / Line 3: @@
 {{Team:Bielefeld-CeBiTec/CSS}}
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+<article> With their broad chemical and functional diversity ncAAs provide a variety of promising applications including proteinlabeling, photocaging, structure analysis and specific protein interactions. Therefore, the development of a new iGEM toolkit for the translational incorporation of non-canonical amino acids (ncAAs) in E. coli would be a great contribution to the iGEM community. The site-specific incorporation of ncAAs requires the directed evolution of tRNA and aminoacyl tRNA synthetase pairs, which in turn mediate the introduction of a ncAA for a certain codon. We are especially exploring the application of unnatural base pairs (UBPs) as an expansion of the genetic code. This approach promises an optimal orthogonality to the autologous translation apparatus and a high flexibility concerning the incorporation of multiple ncAAs. We will utilize several systems to achieve a high retention efficiency. As examples for the successful incorporation of non-canonical amino acids we will develop a rapid test for prions and a new chromatography method for a mild elution of proteins. </article>
-<div class="column full_size">
-<h1>Description</h1>
-<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
-<h5>What should this page contain?</h5>
-<ul>
-<li> A clear and concise description of your project.</li>
-<li>A detailed explanation of why your team chose to work on this particular project.</li>
-<li>References and sources to document your research.</li>
-<li>Use illustrations and other visual resources to explain your project.</li>
-</ul>
-</div>
-<div class="column full_size" >
-<h5>Advice on writing your Project Description</h5>
-<p>
-We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.
-</p>
-<p>
-Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
-</p>
-</div>
-<div class="column half_size" >
-<h5>References</h5>
-<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
-</div>
-<div class="column half_size" >
-<h5>Inspiration</h5>
-<p>See how other teams have described and presented their projects: </p>
-<ul>
-<li><a href="https://2016.igem.org/Team:Imperial_College/Description">2016 Imperial College</a></li>
-<li><a href="https://2016.igem.org/Team:Wageningen_UR/Description">2016 Wageningen UR</a></li>
-<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> 2014 UC Davis</a></li>
-<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">2014 SYSU Software</a></li>
-</ul>
-</div>
 </html>
-{{Team:Bielefeld-CeBiTec/Footer}}

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Revision as of 09:10, 23 June 2017

Bielefeld-CeBiTec