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Revision as of 10:26, 29 October 2017

  1. BioBrick Transformations
  2. Plasmid Isolation
  3. PCRs
  4. Restriction digests
  5. Ligations
  6. Screening

BioBrick Transformations

Plasmid Isolation

PCRs: Adding Restriction Sites and Linkers

Restriction Digests

Multi-Ligation: Coming Full Circle

Screening Transformants: Colony PCR with VF2/VR