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− | <div class="content-section-a">
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− | <div class="container">
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− | <div>
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− | <div class="clearfix"></div>
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− | <h2 class="section-heading">Cell measurements </h2>
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− | <p class="lead">Two colonies from each transformation were picked, and grown in foil-covered 50 ml falcon tubes over night (18 hours). </p>
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− | <p class="lead"><strong>Preparation</strong> OD was measured, and a dilution was calculated to achieve an OD of 0.02. Dilution calculations can be found in the table next to this. Here we used the calculated correction factor from our initial abs/OD calibration. From the absorption measurements taken at 0 hours, we see indications of pipetting errors, as the OD<sub>600</sub> (average) ranges from 0.015 to 0.6 (table 1). </p>
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− | <button onclick="javascript:showhide('InterLab-table')">Show table 1</button> | + | <div class="content-section-b" id="top"> |
− | <div id="InterLab-table" style="display:none;">
| + | <div class="container"> |
− | <div class="container"> | + | |
− | <table class="responsive-table">
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− | <caption><b>Table 1</b> Starting OD.</caption>
| + | |
− | <thead>
| + | |
− | <tr>
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− | <th scope="col"></th>
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− | <th scope="col">Abs<sub>600</sub></th>
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− | <th scope="col">OD<sub>600</sub> before</th>
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− | <th scope="col">Volume (ml) added to 12 ml media</th>
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− | <th scope="col">OD<sub>600</sub> after dilution (0 hours)</th>
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− | </tr>
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− | </thead>
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− | <tfoot>
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− | </tfoot>
| + | |
− | <tbody>
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− | <tr>
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− | <th scope="row">Negative Control (Colony 1)</th>
| + | |
− | <td data-title="Abs600">0,461</td>
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− | <td data-title="OD600 before">1,435</td>
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− | <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,167</td>
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− | <td data-title="OD600 after dilution (0 hours)" data-type="currency">0,06483974</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <th scope="row">Negative Control (Colony 2)</th>
| + | |
− | <td data-title="Abs600">0,463</td>
| + | |
− | <td data-title="OD600 before">1,439</td>
| + | |
− | <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,167</td>
| + | |
− | <td data-title="OD600 after dilution (0 hours)" data-type="currency">0,02584249</td>
| + | |
− | </tr>
| + | |
− | <tr>
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− | <th scope="row">Positive Control (Colony 1)</th>
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− | <td data-title="Abs<sub>600</sub>">0,450</td>
| + | |
− | <td data-title="OD<sub>600</sub> before">1,400</td>
| + | |
− | <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,171</td>
| + | |
− | <td data-title="OD<sub>600</sub> after dilution (0 hours)" data-type="currency">0,04467949</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <th scope="row">Positive Control (Colony 2)</th>
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− | <td data-title="Abs<sub>600</sub>">0,474</td>
| + | |
− | <td data-title="OD<sub>600</sub> before">1,475</td>
| + | |
− | <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,163</td>
| + | |
− | <td data-title="OD<sub>600</sub> after dilution (0 hours)" data-type="currency">0,01689103</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <th scope="row">Test Device 1: J23101+I13504 (Colony 1)</th>
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− | <td data-title="Abs<sub>600</sub>">0,462</td>
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− | <td data-title="OD<sub>600</sub> before">1,437</td>
| + | |
− | <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,167</td>
| + | |
− | <td data-title="OD<sub>600</sub> after dilution (0 hours)" data-type="currency">0,03043498</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <th scope="row">Test Device 1: J23101+I13504 (Colony 2)</th>
| + | |
− | <td data-title="Abs<sub>600</sub>">0,472</td>
| + | |
− | <td data-title="OD<sub>600</sub> before">1,469</td>
| + | |
− | <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,163</td>
| + | |
− | <td data-title="OD<sub>600</sub> after dilution (0 hours)" data-type="currency">0,01977106</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <th scope="row">Test Device 2: J23106+I13504 (Colony 1)</th>
| + | |
− | <td data-title="Abs<sub>600</sub>">0,477</td>
| + | |
− | <td data-title="OD<sub>600</sub> before">1,483</td>
| + | |
− | <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,162</td>
| + | |
− | <td data-title="OD<sub>600</sub> after dilution (0 hours)" data-type="currency">0,03175824</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <th scope="row">Test Device 2: J23106+I13504 (Colony 2)</th>
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− | <td data-title="Abs<sub>600</sub>">0,454</td>
| + | |
− | <td data-title="OD<sub>600</sub> before">1,410</td>
| + | |
− | <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,170</td>
| + | |
− | <td data-title="OD<sub>600</sub> after dilution (0 hours)" data-type="currency">0,018837</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <th scope="row">Test Device 3: J23117+I13504 (Colony 1)</th>
| + | |
− | <td data-title="Abs<sub>600</sub>">0,507</td>
| + | |
− | <td data-title="OD<sub>600</sub> before">1,577</td>
| + | |
− | <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,152</td>
| + | |
− | <td data-title="OD<sub>600</sub> after dilution (0 hours)" data-type="currency">0,03261447</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <th scope="row">Test Device 3: J23117+I13504 (Colony 2)</th>
| + | |
− | <td data-title="Abs<sub>600</sub>">0,492</td>
| + | |
− | <td data-title="OD<sub>600</sub> before">1,529</td>
| + | |
− | <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,157</td>
| + | |
− | <td data-title="OD<sub>600</sub> after dilution (0 hours)" data-type="currency">0,01471154</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <th scope="row">Test Device 4: J23101.BCD2.E0040.B0015 (Colony 1)</th>
| + | |
− | <td data-title="Abs<sub>600</sub>">0,420</td>
| + | |
− | <td data-title="OD<sub>600</sub> before">1,307</td>
| + | |
− | <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,184</td>
| + | |
− | <td data-title="OD<sub>600</sub> after dilution (0 hours)" data-type="currency">0,03759615</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <th scope="row">Test Device 4: J23101.BCD2.E0040.B0015 (Colony 2)</th>
| + | |
− | <td data-title="Abs600">0,472</td>
| + | |
− | <td data-title="OD600 before">1,468</td>
| + | |
− | <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,163</td>
| + | |
− | <td data-title="OD600 after dilution (0 hours)" data-type="currency">0,01860348</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <th scope="row">Test Device 5: J23106.BCD2.E0040.B0015 (Colony 1)</th>
| + | |
− | <td data-title="Abs600">0,513</td>
| + | |
− | <td data-title="OD600 before">1,595</td>
| + | |
− | <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,150</td>
| + | |
− | <td data-title="OD600 after dilution (0 hours)" data-type="currency">0,04234432</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <th scope="row">Test Device 5: J23106.BCD2.E0040.B0015 (Colony 2)</th>
| + | |
− | <td data-title="Abs600">0,467</td>
| + | |
− | <td data-title="OD600 before">1,451</td>
| + | |
− | <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,165</td>
| + | |
− | <td data-title="OD600 after dilution (0 hours)" data-type="currency">0,01494505</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <th scope="row">Test Device 6: J23117.BCD2.E0040.B0015 (Colony 1)</th>
| + | |
− | <td data-title="Abs600">0,482</td>
| + | |
− | <td data-title="OD600 before">1,498</td>
| + | |
− | <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,160</td>
| + | |
− | <td data-title="OD600 after dilution (0 hours)" data-type="currency">0,04452381</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <th scope="row">Test Device 6: J23117.BCD2.E0040.B0015 (Colony 2)</th>
| + | |
− | <td data-title="Abs600">0,455</td>
| + | |
− | <td data-title="OD600 before">1,416</td>
| + | |
− | <td data-title="Volume (ml) added to 12 ml media" data-type="currency">0,170</td>
| + | |
− | <td data-title="OD600 after dilution (0 hours)" data-type="currency">0,02008242</td>
| + | |
− | </tr>
| + | |
− | </tbody>
| + | |
− | </table>
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− | </div>
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− | </div>
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− | </div> | + | <div class="col-lg-5 col-sm-6"> |
− | </div> | + | <hr class="section-heading-spacer"> |
| + | <div class="clearfix"></div> |
| + | <h2 class="section-heading">Laboratory work</h2> |
| + | <p class="lead"> All team members have completed laboratory safety training and are acquainted with regulations relevant to our work. We are confident in our ability to prevent and respond to safety risks and adhere to all hygiene, clothing and behaviour requirements specific to the context of our work. |
| + | <br><br> |
| + | </p> |
| + | </div> |
| + | <div class="col-lg-5 col-lg-offset-2 col-sm-6"> |
| + | <figure> |
| + | <br><br> |
| + | <br><br> |
| + | <img class="img-responsive" src="https://static.igem.org/mediawiki/2017/4/49/Labwaste.jpg" alt="" width="250" height="200"> |
| + | |
| + | <figcaption><b>Image 2 </b>Disposal of plastic waste handled by university.</figcaption> |
| + | </figure> |
| + | </div> |
| + | </div> |
| + | |
| + | </div> |
| + | <!-- /.container --> |
| </div> | | </div> |
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