Difference between revisions of "Team:ETH Zurich/Circuit"

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     <figure class="fig-nonfloat">
 
     <figure class="fig-nonfloat">
 
         <img alt="CATE's Genetic Circuit"  
 
         <img alt="CATE's Genetic Circuit"  
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         src="https://static.igem.org/mediawiki/2017/0/02/T--ETH_Zurich--the-circuit.png"/>
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         src="https://static.igem.org/mediawiki/2017/7/7f/T--ETH_Zurich--TC.png"/>
 
         <figcaption>CATE's Genetic Circuit</figcaption>
 
         <figcaption>CATE's Genetic Circuit</figcaption>
 
     </figure>
 
     </figure>
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    <h2>FIXME with hover over</h2>
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<img alt="Fa"
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        src="https://static.igem.org/mediawiki/2017/a/a2/T--ETH_Zurich--Fa.png"/>
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<img alt="CATE's Genetic Circuit"
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        src="https://static.igem.org/mediawiki/2017/2/25/T--ETH_Zurich--Fb.png"/>
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<img alt="CATE's Genetic Circuit"
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        src="https://static.igem.org/mediawiki/2017/1/18/T--ETH_Zurich--Fc.png"/>
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<img alt="CATE's Genetic Circuit"
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        src="https://static.igem.org/mediawiki/2017/9/99/T--ETH_Zurich--Fd.png"/>
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<img alt="CATE's Genetic Circuit"
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        src="https://static.igem.org/mediawiki/2017/d/d8/T--ETH_Zurich--Fe.png"/>
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     <p>The genetic parts are located on two plasmids, the actuator plasmid and the regulator plasmid. On the regulator plasmid, genes used for control of the behavior are located. These include LuxR, lldP and lldR (all part of <a href="https://2017.igem.org/Team:ETH_Zurich/Circuit/Fa_Tumor_Sensor">Tumor Sensor</a>) and TlpA (<a href="https://2017.igem.org/Team:ETH_Zurich/Circuit/Fd_Heat_Sensor">Heat Sensor</a>). Being combined in an operon on a single plasmid, it makes it easy to manipulate coding regions and fine-tune the gene expression level. The actuator plasmid contains genes for the actions CATE can take. The genes for bacterioferritin (<a href="https://2017.igem.org/Team:ETH_Zurich/Circuit/Fb_MRI_Contrast_Agent">MRI Contrast Agent</a>), azurin (<a href="https://2017.igem.org/Team:ETH_Zurich/Circuit/Fc_Anti_Cancer_Toxin">Anti-Cancer Toxin</a>) and protein E (<a href="https://2017.igem.org/Team:ETH_Zurich/Circuit/Fe_Cell_Lysis">Cell Lysis</a>) are located on this plasmid. Additionally, due to the function of the quorum sensing (part of <a href="https://2017.igem.org/Team:ETH_Zurich/Circuit/Fa_Tumor_Sensor">Tumor Sensor</a>), also the gene for LuxI is on the actuator plasmid. In general, the regulator plasmid contains the sensors to integrate inputs from the outside world, whereas the actuator plasmid produces molecules that act on the environment.</p>
 
     <p>The genetic parts are located on two plasmids, the actuator plasmid and the regulator plasmid. On the regulator plasmid, genes used for control of the behavior are located. These include LuxR, lldP and lldR (all part of <a href="https://2017.igem.org/Team:ETH_Zurich/Circuit/Fa_Tumor_Sensor">Tumor Sensor</a>) and TlpA (<a href="https://2017.igem.org/Team:ETH_Zurich/Circuit/Fd_Heat_Sensor">Heat Sensor</a>). Being combined in an operon on a single plasmid, it makes it easy to manipulate coding regions and fine-tune the gene expression level. The actuator plasmid contains genes for the actions CATE can take. The genes for bacterioferritin (<a href="https://2017.igem.org/Team:ETH_Zurich/Circuit/Fb_MRI_Contrast_Agent">MRI Contrast Agent</a>), azurin (<a href="https://2017.igem.org/Team:ETH_Zurich/Circuit/Fc_Anti_Cancer_Toxin">Anti-Cancer Toxin</a>) and protein E (<a href="https://2017.igem.org/Team:ETH_Zurich/Circuit/Fe_Cell_Lysis">Cell Lysis</a>) are located on this plasmid. Additionally, due to the function of the quorum sensing (part of <a href="https://2017.igem.org/Team:ETH_Zurich/Circuit/Fa_Tumor_Sensor">Tumor Sensor</a>), also the gene for LuxI is on the actuator plasmid. In general, the regulator plasmid contains the sensors to integrate inputs from the outside world, whereas the actuator plasmid produces molecules that act on the environment.</p>

Revision as of 14:05, 30 October 2017

Circuit

Overview

In synthetic biology, everything is about genetic circuits and biological information processing. For CATE we specifically designed a circuit, able to integrate signals from the outside and the inside. It features two safety checkpoints to ensure superiority in terms of off-target damage and controllability if compared to conventional cancer treatment strategies.

CATE's Genetic Circuit
CATE's Genetic Circuit

FIXME with hover over

Fa CATE's Genetic Circuit CATE's Genetic Circuit CATE's Genetic Circuit CATE's Genetic Circuit

The genetic parts are located on two plasmids, the actuator plasmid and the regulator plasmid. On the regulator plasmid, genes used for control of the behavior are located. These include LuxR, lldP and lldR (all part of Tumor Sensor) and TlpA (Heat Sensor). Being combined in an operon on a single plasmid, it makes it easy to manipulate coding regions and fine-tune the gene expression level. The actuator plasmid contains genes for the actions CATE can take. The genes for bacterioferritin (MRI Contrast Agent), azurin (Anti-Cancer Toxin) and protein E (Cell Lysis) are located on this plasmid. Additionally, due to the function of the quorum sensing (part of Tumor Sensor), also the gene for LuxI is on the actuator plasmid. In general, the regulator plasmid contains the sensors to integrate inputs from the outside world, whereas the actuator plasmid produces molecules that act on the environment.

Illustration

Description of the genetic procedure