Difference between revisions of "Team:Aix-Marseille/Project"

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[[File:T--Aix-Marseille--KX.png|500px|right|thumb|KILL XYL project.]]
 
[[File:T--Aix-Marseille--KX.png|500px|right|thumb|KILL XYL project.]]
  
Our project '''KILL XYL''' is a cure against the disease caused by the plant pathogen [[Team:Aix-Marseille/Xylella_fastidiosa|''Xylella fastidiosa'']]. This disease currently causes the loss of thousands of hectares of European crops and no extensive cure exists against it.
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Our project '''KILL XYL''' is to find a cure for the disease caused by the plant pathogen [[Team:Aix-Marseille/Xylella_fastidiosa|''Xylella fastidiosa'']]. This disease currently causes the loss of thousands of acres of European crops and currently there is no cure for it.
 
   
 
   
At Aix-Marseille University we thought about a solution that encloses many aspects of the cure. First, we wanted to improve the [[Team:Aix-Marseille/Hardware|detection]] of the disease. To do so we use a NDVI camera that will help us to see if the plant does photosynthesis or not. If the plant is heavily affected by [[Team:Aix-Marseille/Xylella_fastidiosa|''Xylella fastidiosa'']], it supports a hydric stress that stops the photosynthesis.
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At Aix-Marseille University we thought about developing a solution that multiple aspects. First, we wanted to improve [[Team:Aix-Marseille/Hardware|detection]] of the disease. To do this we use an NDVI camera that will help us to see if the plant is stressed or not. If the plant is infected by [[Team:Aix-Marseille/Xylella_fastidiosa|''Xylella fastidiosa'']], it suffers from a hydric stress that stops the photosynthesis.
  
Secondly, we want to get rid of the bacterium. Phages are natural predators of bacteria. They can also be used to transfect DNA into a bacterial cell. Phages have also the advantage of being specific to a strain and to be modulable. As we wanted to be eco-friendly, we create [[Team:Aix-Marseille/Bacteriophages|phage-like particles]] (PLPs), that aren't able to spread. Thus we have phage specific to [[Team:Aix-Marseille/Xylella_fastidiosa|''X. fastidiosa'']], capable to inject toxic genes into the bacterium.
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Secondly, we want to get rid of the bacteria.  
 +
Phages are natural predators of bacteria.  
 +
They can also be used to transfer DNA into bacteria.  
 +
Phages have the additional advantages of being strain specific and modulable.  
 +
As we wanted an eco-friendly treatment,  
 +
that might easilly obtain [[Team:Aix-Marseille/Xylella_fastidiosa|authorizations]] for marketing,
 +
we decided to create [[Team:Aix-Marseille/Bacteriophages|phage-like particles]] (PLPs), that aren't able to spread.  
 +
So we will construct phage specific to [[Team:Aix-Marseille/Xylella_fastidiosa|''X. fastidiosa'']],  
 +
capable of injecting toxic genes into the bacteria.
  
 
The main cause of the plants' death, is the hydric stress induced by the accumulation of biofilm into the xylem vessels. To disrupt the biofilm we thought about different solutions. The first one is to stop the bacterium producing any extra poly-saccharide. This could be achieved by [[Team:Aix-Marseille/QS|quenching the quorum sensing]] of the bacterium with the help of a little fatty acid called: 2-cis-decenoic acid. Secondly, we wanted to destroy the exo-polysaccharides. An [[Team:Aix-Marseille/DEPS|enzyme]] coming from a bacteriophage could fulfil the use by the hydrolysis of polysaccharides.  
 
The main cause of the plants' death, is the hydric stress induced by the accumulation of biofilm into the xylem vessels. To disrupt the biofilm we thought about different solutions. The first one is to stop the bacterium producing any extra poly-saccharide. This could be achieved by [[Team:Aix-Marseille/QS|quenching the quorum sensing]] of the bacterium with the help of a little fatty acid called: 2-cis-decenoic acid. Secondly, we wanted to destroy the exo-polysaccharides. An [[Team:Aix-Marseille/DEPS|enzyme]] coming from a bacteriophage could fulfil the use by the hydrolysis of polysaccharides.  

Revision as of 15:40, 31 October 2017

KILL XYL

KILL XYL project.

Our project KILL XYL is to find a cure for the disease caused by the plant pathogen Xylella fastidiosa. This disease currently causes the loss of thousands of acres of European crops and currently there is no cure for it.

At Aix-Marseille University we thought about developing a solution that multiple aspects. First, we wanted to improve detection of the disease. To do this we use an NDVI camera that will help us to see if the plant is stressed or not. If the plant is infected by Xylella fastidiosa, it suffers from a hydric stress that stops the photosynthesis.

Secondly, we want to get rid of the bacteria. Phages are natural predators of bacteria. They can also be used to transfer DNA into bacteria. Phages have the additional advantages of being strain specific and modulable. As we wanted an eco-friendly treatment, that might easilly obtain authorizations for marketing, we decided to create phage-like particles (PLPs), that aren't able to spread. So we will construct phage specific to X. fastidiosa, capable of injecting toxic genes into the bacteria.

The main cause of the plants' death, is the hydric stress induced by the accumulation of biofilm into the xylem vessels. To disrupt the biofilm we thought about different solutions. The first one is to stop the bacterium producing any extra poly-saccharide. This could be achieved by quenching the quorum sensing of the bacterium with the help of a little fatty acid called: 2-cis-decenoic acid. Secondly, we wanted to destroy the exo-polysaccharides. An enzyme coming from a bacteriophage could fulfil the use by the hydrolysis of polysaccharides.

Hence, KILL XYL simply detects, disrupt and kill Xylella fastidiosa.

  • T--Aix-Marseille--drone.pngDetection
  • T--Aix-Marseille--icon-phage.pngEngineering PLPs
  • T--Aix-Marseille--icon-QS.pngQuorum sensing
  • T--Aix-Marseille--icon-deps.pngDisrupting biofilm
  • T--Aix-Marseille--icon-model.pngModelling