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<a href="#"><img src="https://static.igem.org/mediawiki/2017/b/bb/T--Stony_Brook--homepage-swords.png" style="text-align: center;width:250px;height:250px;"/></a> | <a href="#"><img src="https://static.igem.org/mediawiki/2017/b/bb/T--Stony_Brook--homepage-swords.png" style="text-align: center;width:250px;height:250px;"/></a> | ||
</header> | </header> | ||
+ | |||
+ | |||
+ | <h4>8/06:</h4> | ||
+ | <p> • Prepared samples of GST A53 and GST LnqZ to run SDS-PAGE</p> | ||
+ | <p> • Ran SDS-PAGE</p> | ||
+ | <p> • Viewed SDS-PAGE→ faint protein bands in elution 1 for both A53 and LnqZ, but not in any other elutions</p> | ||
+ | |||
+ | |||
+ | <h4>8/07:</h4> | ||
+ | <p> • Nanodropped our elutions of MBP protein purification products</p> | ||
+ | <a href="#"><img src="https://static.igem.org/mediawiki/2017/f/f3/T--Stony_Brook--11.1.jpg" style="text-align: center;width:250px;height:250px;"/></a> | ||
+ | <p> • Nanodropped our elutions of GST protein purification products</p> | ||
+ | <a href="#"><img src="https://static.igem.org/mediawiki/2017/3/3c/T--Stony_Brook--11.2.jpg" style="text-align: center;width:250px;height:250px;"/></a> | ||
+ | <a href="#"><img src="https://static.igem.org/mediawiki/2017/2/26/T--Stony_Brook--11.3.jpg" style="text-align: center;width:250px;height:250px;"/></a> | ||
+ | <p> • Freeze-Thaw of SUMO LnqZ and Epi</p> | ||
+ | <p> • Made liquid cultures of</p> | ||
+ | <p> • MBP lnqZE</p> | ||
+ | <p> • MBP lnqZA</p> | ||
+ | <p> • MBP lnqZQ</p> | ||
+ | |||
+ | <h4>8/08:</h4> | ||
+ | <p> • Nanodrop elutions of SUMO Epi</p> | ||
+ | <a href="#"><img src="https://static.igem.org/mediawiki/2017/d/de/T--Stony_Brook--11.4.jpg" style="text-align: center;width:250px;height:250px;"/></a> | ||
+ | <p> • Nanodrop elutions of SUMO Lnq Z</p> | ||
+ | <a href="#"><img src="https://static.igem.org/mediawiki/2017/9/9f/T--Stony_Brook--11.5.jpg" style="text-align: center;width:250px;height:250px;"/></a> | ||
+ | |||
+ | <h4>8/09:</h4> | ||
+ | <p> • Continued expression and purification of our proteins.</p> | ||
+ | |||
+ | <h4>8/09:</h4> | ||
+ | <p> • Continued expression and purification of our proteins.</p> | ||
+ | <p> • Discussed improvements on our purification protocol with our advisor Dr. Steven Glynn. | ||
+ | </p> | ||
+ | |||
+ | <h4>8/09:</h4> | ||
+ | <p> • Continued expression and purification of our proteins.</p> | ||
+ | <p> • Applied new improvements to our purification protocol, such as the use of a sonicator </p> | ||
+ | |||
+ | </section> | ||
Revision as of 00:28, 1 November 2017