Difference between revisions of "Team:Stony Brook/Notebook/Week11"

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<h4>8/06:</h4>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&#8226;&nbsp;Prepared samples of GST A53 and GST LnqZ to run SDS-PAGE</p>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&#8226;&nbsp;Ran SDS-PAGE</p>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&#8226;&nbsp;Viewed SDS-PAGE→ faint protein bands in elution 1 for both A53 and LnqZ, but not in any other elutions</p>
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<h4>8/07:</h4>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&#8226;&nbsp;Nanodropped our elutions of MBP protein purification products</p>
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<a href="#"><img src="https://static.igem.org/mediawiki/2017/f/f3/T--Stony_Brook--11.1.jpg" style="text-align: center;width:250px;height:250px;"/></a>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&#8226;&nbsp;Nanodropped our elutions of GST protein purification products</p>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&#8226;&nbsp;Freeze-Thaw of SUMO LnqZ and Epi</p>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&#8226;&nbsp;Made liquid cultures of</p>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&#8226;&nbsp;MBP lnqZE</p>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&#8226;&nbsp;MBP lnqZA</p>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&#8226;&nbsp;MBP lnqZQ</p>
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<h4>8/08:</h4>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&#8226;&nbsp;Nanodrop elutions of SUMO Epi</p>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&#8226;&nbsp;Nanodrop elutions of SUMO Lnq Z</p>
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<a href="#"><img src="https://static.igem.org/mediawiki/2017/9/9f/T--Stony_Brook--11.5.jpg" style="text-align: center;width:250px;height:250px;"/></a>
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<h4>8/09:</h4>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&#8226;&nbsp;Continued expression and purification of our proteins.</p>
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<h4>8/09:</h4>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&#8226;&nbsp;Continued expression and purification of our proteins.</p>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&#8226;&nbsp;Discussed improvements on our purification protocol with our advisor Dr. Steven Glynn.
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</p>
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<h4>8/09:</h4>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&#8226;&nbsp;Continued expression and purification of our proteins.</p>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&#8226;&nbsp;Applied new improvements to our purification protocol, such as the use of a sonicator </p>
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</section>
  
  

Revision as of 00:28, 1 November 2017

Stony Brook 2017