Difference between revisions of "Team:Stony Brook/Notebook/Week11"
| Line 92: | Line 92: | ||
<h4>8/07:</h4> | <h4>8/07:</h4> | ||
<p> • Nanodropped our elutions of MBP protein purification products</p> | <p> • Nanodropped our elutions of MBP protein purification products</p> | ||
| − | <a href="#"><img src="https://static.igem.org/mediawiki/2017/f/f3/T--Stony_Brook--11.1.jpg" style="text-align: center;width: | + | <a href="#"><img src="https://static.igem.org/mediawiki/2017/f/f3/T--Stony_Brook--11.1.jpg" style="text-align: center;width:600px;height:600px;"/></a> |
<p> • Nanodropped our elutions of GST protein purification products</p> | <p> • Nanodropped our elutions of GST protein purification products</p> | ||
<a href="#"><img src="https://static.igem.org/mediawiki/2017/3/3c/T--Stony_Brook--11.2.jpg" style="text-align: center;width:600px;height:500px;"/></a> | <a href="#"><img src="https://static.igem.org/mediawiki/2017/3/3c/T--Stony_Brook--11.2.jpg" style="text-align: center;width:600px;height:500px;"/></a> | ||
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<h4>8/08:</h4> | <h4>8/08:</h4> | ||
<p> • Nanodrop elutions of SUMO Epi</p> | <p> • Nanodrop elutions of SUMO Epi</p> | ||
| − | <a href="#"><img src="https://static.igem.org/mediawiki/2017/d/de/T--Stony_Brook--11.4.jpg" style="text-align: center;width: | + | <a href="#"><img src="https://static.igem.org/mediawiki/2017/d/de/T--Stony_Brook--11.4.jpg" style="text-align: center;width:600px;height:600px;"/></a> |
<p> • Nanodrop elutions of SUMO Lnq Z</p> | <p> • Nanodrop elutions of SUMO Lnq Z</p> | ||
| − | <a href="#"><img src="https://static.igem.org/mediawiki/2017/9/9f/T--Stony_Brook--11.5.jpg" style="text-align: center;width: | + | <a href="#"><img src="https://static.igem.org/mediawiki/2017/9/9f/T--Stony_Brook--11.5.jpg" style="text-align: center;width:600px;height:600px;"/></a> |
<h4>8/09:</h4> | <h4>8/09:</h4> | ||
Latest revision as of 02:11, 1 November 2017
Week 11
8/06:
• Prepared samples of GST A53 and GST LnqZ to run SDS-PAGE
• Ran SDS-PAGE
• Viewed SDS-PAGE→ faint protein bands in elution 1 for both A53 and LnqZ, but not in any other elutions
8/07:
• Nanodropped our elutions of MBP protein purification products
• Nanodropped our elutions of GST protein purification products
• Freeze-Thaw of SUMO LnqZ and Epi
• Made liquid cultures of
• MBP lnqZE
• MBP lnqZA
• MBP lnqZQ
8/08:
• Nanodrop elutions of SUMO Epi
• Nanodrop elutions of SUMO Lnq Z
8/09:
• Continued expression and purification of our proteins.
8/10:
• Continued expression and purification of our proteins.
• Discussed improvements on our purification protocol with our advisor Dr. Steven Glynn.
8/11:
• Continued expression and purification of our proteins.
• Applied new improvements to our purification protocol, such as the use of a sonicator
