Difference between revisions of "Team:Stony Brook/Notebook/Week12"
| Line 89: | Line 89: | ||
<h4>8/15:</h4> | <h4>8/15:</h4> | ||
<p> • Nanodrop of MBP lnqZE Elutions</p> | <p> • Nanodrop of MBP lnqZE Elutions</p> | ||
| − | <a href="#"><img src="https://static.igem.org/mediawiki/2017/b/b5/T--Stony_Brook--12.1.jpg" style="text-align: center;width: | + | <a href="#"><img src="https://static.igem.org/mediawiki/2017/b/b5/T--Stony_Brook--12.1.jpg" style="text-align: center;width:700px;height:500px;"/></a> |
<p> • Nanodrop of MBP lnqZA Elutions</p> | <p> • Nanodrop of MBP lnqZA Elutions</p> | ||
| − | <a href="#"><img src="https://static.igem.org/mediawiki/2017/9/91/T--Stony_Brook--12.2.jpg" style="text-align: center;width: | + | <a href="#"><img src="https://static.igem.org/mediawiki/2017/9/91/T--Stony_Brook--12.2.jpg" style="text-align: center;width:700px;height:500px;"/></a> |
<p> • Nanodrop of MBP lnqZQ Elutions</p> | <p> • Nanodrop of MBP lnqZQ Elutions</p> | ||
| − | <a href="#"><img src="https://static.igem.org/mediawiki/2017/a/ac/T--Stony_Brook--12.3.jpg" style="text-align: center;width: | + | <a href="#"><img src="https://static.igem.org/mediawiki/2017/a/ac/T--Stony_Brook--12.3.jpg" style="text-align: center;width:700px;height:500px;"/></a> |
<h4>8/16:</h4> | <h4>8/16:</h4> | ||
<p> • Nanodrop of GST A53 Elutions</p> | <p> • Nanodrop of GST A53 Elutions</p> | ||
| − | <a href="#"><img src="https://static.igem.org/mediawiki/2017/e/e1/T--Stony_Brook--12.4.jpg" style="text-align: center;width: | + | <a href="#"><img src="https://static.igem.org/mediawiki/2017/e/e1/T--Stony_Brook--12.4.jpg" style="text-align: center;width:700px;height:500px;"/></a> |
<p> • Nanodrop of GST Elutions</p> | <p> • Nanodrop of GST Elutions</p> | ||
| − | <a href="#"><img src="https://static.igem.org/mediawiki/2017/2/21/T--Stony_Brook--12.5.jpg" style="text-align: center;width: | + | <a href="#"><img src="https://static.igem.org/mediawiki/2017/2/21/T--Stony_Brook--12.5.jpg" style="text-align: center;width:700px;height:500px;"/></a> |
<p> • Nanodrop of MBP lnqZA hybrid Elutions</p> | <p> • Nanodrop of MBP lnqZA hybrid Elutions</p> | ||
| − | <a href="#"><img src="https://static.igem.org/mediawiki/2017/b/b4/T--Stony_Brook--12.6.jpg" style="text-align: center;width: | + | <a href="#"><img src="https://static.igem.org/mediawiki/2017/b/b4/T--Stony_Brook--12.6.jpg" style="text-align: center;width:700px;height:500px;"/></a> |
<p> • Ran an SDS Page gel to determine whether or not the protein was being synthesized/ where in the purification process it was being collected</p> | <p> • Ran an SDS Page gel to determine whether or not the protein was being synthesized/ where in the purification process it was being collected</p> | ||
<p> • Tested elutions of GST Epi and MBP lnqZA</p> | <p> • Tested elutions of GST Epi and MBP lnqZA</p> | ||
| − | <a href="#"><img src="https://static.igem.org/mediawiki/2017/a/ac/T--Stony_Brook--12.7.jpg" style="text-align: center;width: | + | <a href="#"><img src="https://static.igem.org/mediawiki/2017/a/ac/T--Stony_Brook--12.7.jpg" style="text-align: center;width:600px;height:700px;"/></a> |
<h4>8/17:</h4> | <h4>8/17:</h4> | ||
| Line 114: | Line 114: | ||
<h4>8/18:</h4> | <h4>8/18:</h4> | ||
<p> • Nandodrop of MBP lnqZE Elutions</p> | <p> • Nandodrop of MBP lnqZE Elutions</p> | ||
| − | <a href="#"><img src="https://static.igem.org/mediawiki/2017/e/e8/T--Stony_Brook--12.8.jpg" style="text-align: center;width: | + | <a href="#"><img src="https://static.igem.org/mediawiki/2017/e/e8/T--Stony_Brook--12.8.jpg" style="text-align: center;width:700px;height:500px;"/></a> |
<p> • Nanodrop of sample 1 of MBP lnqZA</p> | <p> • Nanodrop of sample 1 of MBP lnqZA</p> | ||
| − | <a href="#"><img src="https://static.igem.org/mediawiki/2017/6/68/T--Stony_Brook--12.9.jpg" style="text-align: center;width: | + | <a href="#"><img src="https://static.igem.org/mediawiki/2017/6/68/T--Stony_Brook--12.9.jpg" style="text-align: center;width:700px;height:500px;"/></a> |
<p> • Nanodrop of sample 2 of MBP lnqZA</p> | <p> • Nanodrop of sample 2 of MBP lnqZA</p> | ||
| − | <a href="#"><img src="https://static.igem.org/mediawiki/2017/b/bb/T--Stony_Brook--12.10.jpg" style="text-align: center;width: | + | <a href="#"><img src="https://static.igem.org/mediawiki/2017/b/bb/T--Stony_Brook--12.10.jpg" style="text-align: center;width:700px;height:500px;"/></a> |
Latest revision as of 02:16, 1 November 2017
Week 12
8/14:
• Continued expression and purification of our proteins.
8/15:
• Nanodrop of MBP lnqZE Elutions
• Nanodrop of MBP lnqZA Elutions
• Nanodrop of MBP lnqZQ Elutions
8/16:
• Nanodrop of GST A53 Elutions
• Nanodrop of GST Elutions
• Nanodrop of MBP lnqZA hybrid Elutions
• Ran an SDS Page gel to determine whether or not the protein was being synthesized/ where in the purification process it was being collected
• Tested elutions of GST Epi and MBP lnqZA
8/17:
• Protein purified MBP lnqZE hybrid, two different samples of MBP lnqZA hybrid, and MBP Lnq ZQ hybrid
8/18:
• Nandodrop of MBP lnqZE Elutions
• Nanodrop of sample 1 of MBP lnqZA
• Nanodrop of sample 2 of MBP lnqZA
