Difference between revisions of "PASantiago Chile/notebook"

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<u>5-Enzyme                      0,5 ul</u>
 
<u>5-Enzyme                      0,5 ul</u>
 
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</pre>  
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<pre>            Total: 20ul</pre>
  
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</p>
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1-H2O Mq  16,3 ul
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<br>
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<pre>2-Buffer MC                            2 ul </pre>
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Revision as of 05:03, 1 November 2017

Notebook

March 2016 to March 2017:

1. We work with BioBricks of previous years (kit iGEM 2015. 2016).
2. We re-suspend genetic material and start to do all the standard methods, like
3. The results are not what expected. We cannot see the material in the gel, doing impossible to prove our biological circuit.

April 2017-July 2017

1.We received the distribution kit 2017.
2.We prove again with all the procedure, but we cannot have the results that expected.

July 11th-13th

1.Review and later shipment of parts to IDT to synthesize (glocks Gene Fragments)
2. The parts were:

  • LuxR + LuxI (Lux)
  • AmilCp + Lemon scent (Module 2)
  • cI repressor + Prm + Or + Pr + Cro (Complete sensor)
  • cI repressor + Prm (Part 1, module 1)
  • Or + Pr + Cro (Part 2, module 1)

    • Saturday 19th, August

    1.Re-suspension of the gBlocks (20ng/ul)
    2.Digestion of parts. (1 hour for 37°C)

  • Complete sensor (SpeI)
  • Lux (XbaI)
  • Plasmid 2 (EcoRI-PstI)
  • Backbone pSB1C3 (EcoRI-PstI)

    • Digestion Protocol: (One enzyme) 

    1-H2O Mq                      16,3 ul
2-Buffer MC                   2    ul 
3-BSA                             0,2 ul 
4-DNA                             1   ul 
5-Enzyme                      0,5 ul

                 Total: 20ul