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Revision as of 12:53, 1 November 2017

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Parts

Full Part table

 Part Number Type Designer Length
Nor Pyochelin Synthesis Cluster BBa_K2401000 Composite A. Nicolai, S. Ricke 10760 bp
Yersiniabactin inner Membrane Transporter BBa_K2401005 Composite A. Nicolai, S. Ricke 3956 bp
Siderophore outer Membrane Transporter BBa_K2401011 Composite A. Nicolai, S. Ricke 5724 bp
HMWP1 Yersiniabactin Synthetase part 2 BBa_K2401012 Composite A. Nicolai, S. Ricke 10731 bp
HMWP2 Yersiniabactin Synthetase Part 1 BBa_K2401001 Basic A. Nicolai, S. Ricke 6107 bp
2,3-Dihydroxybenzoate-AMP ligase BBa_K2401002 Basic A. Nicolai, S. Ricke 1578 bp
Thiazolinyl imide reductase BBa_K2401003 Basic A. Nicolai, S. Ricke 1101 bp
Surfactin imide reductase BBa_K2401004 Basic A. Nicolai, S. Ricke 498 bp
ABC-Siderophore-Transporter BBa_K2401006 Basic A. Nicolai, S. Ricke 1789 bp
ATP-Siderophore-Bindingpermease BBa_K2401007 Basic A. Nicolai, S. Ricke 1801 bp
HMWP1 Yersiniabactin Synthetase Part 2 BBa_K2401008 Basic A. Nicolai, S. Ricke 9492 bp
FyuA TonB Yersiniabactin outermembrane Receptor BBa_K2401010 Basic A. Nicolai, S. Ricke 2022 bp
FptA Pyochelin outermembrane Receptor BBa_K2401009 Basic A. Nicolai, S. Ricke 2160 bp
PchR Pyochelin regulator BBa_K2401013 Regulatory A. Nicolai, S. Ricke 960 bp
AraC like Yersiniabactin regulator BBa_K2401014 Regulatory A. Nicolai, S. Ricke 891 bp

Basic Parts

AraC family transcriptional regulator [AraCftr]
Origin: Escherichia coli
Data: 891 bp, 296 AS, 32,3 kDa
Part No.: BBa_K2401014
Function: Yersiniabactin-Complex Regulated Promotor, most likely a leaky promoter that induces the Yersiniabactin synthesis if Yersiniabactin-iron3+-complex binds to the receptor. Most siderophore receptor complexes need to be leaky since siderophores are only necessary under certain circumstances and self-induced. So, in the context of siderophores, leakiness is no flaw but a feature, including a biosensor for iron3+-ions in the expression system which makes sure that unnecessary metabolic load is exhibited. Therefore, the iron acquisition method is highly efficient and only expressed when the circumstances demand it.
Plasmid: Singular on a plasmid (pSB1C3) with cAMP-resistance.
Regulatory protein PchR [PchR]
Origin: Pseudomonas aeruginosa PAO1 (strain: PAO1)
Data: 960 bp, 319 AS, 35.9 kDa
Part No.: BBa_K2401013
Function: Pyochelin-Complex Regulated Promotor, most likely a leaky promoter that induces the Pyochelin synthesis if Pyochelin-iron3+-di-complex binds to the receptor. Most siderophore receptor complexes need to be leaky since siderophores are only necessary under certain circumstances and self-induced. So, in the context of siderophores, leakiness is no flaw but a feature, including a biosensor for iron3+-ions in the expression system which makes sure that unnecessary metabolic load is exhibited. Therefore, the iron acquisition method is highly efficient and only expressed when the circumstances demand it. Our goal was to characterize the promoter and measure if pyochelin-gallium3+ and Nor-pyochelin-gallium3+ complexes induce the native promoter to estimate the metabolic excess overload that our antibiotic-compound induces on native Pseudomonas aeruginosa.
Plasmid: Singular: pSB1C3 with cAMP-resistance. Combination: With HMWP2 , tired, sfp and dhBe to mimic the native expression system for pyochelin to measure additional metabolic load.

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Composite Parts


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