Team:Hamburg/Attributions

Attributions

Team Members

Team leader

Shanti Ricke

Shanti was one of this year’s team leaders and engaged with the development and execution of our project idea. He chose our target organisms Pseudomonas aeruginosa and Klebsiella pneumoniae and the corresponding siderophores and genes. Furthermore, he designed our BioBricks and spend most his time in both the S1 and S2 labs to supervise and perform the experiments. His responsibilities included the organisation of the chemistry labs and the S2 lab and the communication with our cooperation partners and guest speakers.
He arranged a lot of the preparations and execution of our Human Practices activities. He also had a part in sponsoring, writing our Wiki and established himself as contact person for everybody. If you have any questions or problem – he’s your man!

Vice-Team leader

Elisabeth Orlowski

Elli was also a team leader mostly in charge of sponsoring, Social Media and lend a hand with our wiki. In the lab she worked in the S1 and S2 life science labs and was responsible for the toxicology tests in the cell culture as well as for the growth inhibition assays with the pseudomonas. For sponsoring she mainly organized the crowd funding campaign and was involved in all legal matters and support for our sponsors.

Alina Nicolai

Alina was one of our two vice team leaders this year. She worked in the S1- and S2- lab, for which she was in charge of designing the parts and developing the project design. She was also involved in sponsoring, our wiki and human practices.

Head of Sponsoring

Valerie Heine

Valerie was our golden girl and sponsoring queen involved in everything organising. She spent every free minute in the labs where she proved herself (basically) irreplaceable. She took care of keeping everything in order and made sure we did not descend into chaos. Besides she found time to work on our wiki (and harassing everybody into keeping the lab books updated) and helped organising our Human Practices activities.

Head of Human Practices

Geoffrey N. Youett

Geoffrey is a true organisation talent and knows probably more people than all our team members together. Consequently, he took charge of our Human Practices, made contacts and wrote application letters for our events. Despite not having a background in science, he joined the lab team and helped in sponsoring and writing of our wiki. He also assisted with several cooperations.

Head of Finances

Ruth Rietow

Ruth has a rare gift for numbers and took care of all our finances and accounting, which she did formidable. She was involved in basically every part of our project and spend a considerable amount of time in the S1 and S2 labs, helped with sponsoring and wrote parts of our wiki. She also kept team spirit alive thanks to her amazing baking skills.

Biochemistry

Guillermo Molina

Guillermo worked in the S1 and S2 lab. He wrote on our wiki and generously shared the chocolate he always had in his coat and that helped to prevent the occasional nervous breakdown. Also without him we would have probably starved, as he always prepared and organized food for everyone.

Annika Soltau

Annika is a treasure in the lab. She kept the work in the S1 lab alive and moved in the S2 lab later. She also helped with the sponsoring too and worked on our wiki.

Desiree Brösamle

Desiree was a real help in the lab. She worked mainly in the S1 lab and took charge of the S2 lab every now and then. She was involved in sponsoring and the wiki and made sure we took a break to have lunch during lab hours.

Franziska Dörr

Franzi worked in the S1 lab and helped with the sponsoring.

Svenja Reich

Svenja was involved in the S1 lab work and helped with the sponsoring and our wiki.

Timo "Kutte" Zeimet

Kutte worked in the S1 and S2 lab and helped with sponsoring and the wiki.

Kamil Grzegorczyk

Kamil contributed the foundation of our project by bringing up the idea of using gallium siderophores as an antibacterial strategy.

Chemistry

Carla von Salisch

Carla performed a chemical synthesis of desferrithiocine, helped in the S1 lab, with sponsoring and managed the ordering of the chemicals.

Jan-Christian Raabe

Jan-Christian synthesised the siderophor nor-pyocheline and performed the complexation with gallium. He also helped in the S1 lab, with sponsoring and writing our wiki.

Martin Borowski

Martin worked on two ways to synthesise the siderophore pyocheline to determine how to achieve the best yield. He was involved in sponsoring and the wiki too.

Nanoscientists

Tim Erichlandwehr

Tim helped to develop the nano part of our project and established the contact to Prof. Trebbin, who supervised the nanoscience project. He also helped with sponsoring.

Nico Brkovic

Nico developed the PDMS chip, the membrane and the microfluidic device with Ramakrishnan, a doctorate student in our supervising group. He also helped in the S1 and S2 labs, with sponsoring and the wiki. He organised the system for the pump and the devices.

Sören Hecht

Sören worked in the S1 lab, helped with the sponsoring and the organising of the pump for the nano experiments. He also wrote part of our wiki and was involved in public relations.

Graphics

Jannes Ernsting

Jannes is part of the graphic design team and was responsible for our web design.

Leona Bruners

Leona is also part of the graphic design team and made the model for our logo, and our famous schokofroschkarten.

Julian Strunck

Julian is another graphic design student who developed the logo with Tom with Leona’s model as template.

Tom Böhl

Tom developed the logo with Julian after Leonas’s template.

Jannes, Leona, Julian and Tom worked together on the design and corporate design and helped us chose our team name. They also worked on our Social Media presentation, took our team photos and filmed the Crowd-founding video. To sum it up: everything that looks good is their work!

Psychology

Frank-Olaf Lohmann

Frank-Olaf was our person to talk to about every possible problem within the team. He is very experienced in the business environment and project planning. With his experience and psychological backround he provided us with many insights regarding team structure, workflow and communications. He also did a retrospective with us to work out what went well and where our project could have needed some improvement.

Our Instructors and Advisors

Primary PI

Zoya Ignatova

zoyaProfessor Zoya Ignatova is supervising iGEM Hamburg for the third time supervising as a primary Principal Investigator of our team. She is professor for biochemistry at the University of Hamburg and specified in RNA biochemistry, in particular on tRNAs. Besides offering us her advice, she provided us with a S1 lab space, the equipment and support of her group. She stood by our side for any questions and problems and gave us a free hand to enable us to develop and execute our own project.

Secondary PI

Andreas Czech

zoya Andreas is for the first time acting as secondary Principal Investigator for the iGEM Team Hamburg. He stood by our side the entire year to give advice, provide us with chemicals and materials and figure out the handling of the plate reader. Furthermore, he supervised our expression test and always helped with any seemingly unsolvable problem!

Frauke Adamla

zoya Frauke is for the second time participating as secondary Principal Investigator. She introduced us to the basics of lab work. Until she left the university in summer she was our first contact person and gave us advice even after she was gone. We are very sad about her leave which is a great loss for us and the following teams. Thank you for always being there for us!

Instructor

Peter Heisig

PHeisig

Peter Heisig was born in 1958 and studied pharmacy at the Free University of Berlin. After his PhD at the Max Planck Institute for Molecular Genetics, he qualified as a professor at the University of Bonn and was appointed professor for pharmaceutical biology and microbiology at the University of Hamburg in 2000. He and his group carry out research on the development, spreading and consequences of antibiotic resistances with a special interest in fluoroquinolones. In addition to being head of the group, he is a safety advisor for the S2 lab and also involved in teaching the pharmacy students.

We converted him to our project when we presented our ideas and won him over as an instructor for our team. He patiently discussed our experiments with us and offered lots of helpful hints and new approaches for the design as well as for troubleshooting. He regularly asked about the progress of our project and always made time for our issues. Furthermore, he invited us to his group and offered us some space in his S2 lab on short notice when our former lab suddenly was closed down and therefore saved us from interrupting our lab work and wasting a lot of time.

Anke Heisig

AHeisig

Anke Heisig, born in 1961, is a biologist. She studied at the Free University of Berlin before she did her PhD at the Max Planck Institute for Molecular Genetics and then moved on to the University of Bonn where she run the DNA sequencing service.

She started working at the University of Hamburg in 2004. Here, she is the head of the DNA analytic service (including next generation sequencing) and safety advisor. Her research focuses on antibiotic resistances.

She warmly welcomed us when we moved to the Heisig’s lab on the spur of the moment and did everything in her power to help us setting up our new working space which included finding us basic equipment that we could borrow until we were able to bring our own. As her husband, she was very interested in our project, became an instructor for our team and always had a sympathetic ear for our questions and problems. She advised us on the design of the experiments and troubleshooting and was of great help especially designing the toxicity tests.

Ignatova Lab

Ign

Ingrid Goebel

Ingrid is a technical assistant in Prof. Ignatova’s and a true treasure. She seems to know the answer to any question and always gives good advice – and most importantly: she knows where to find literally everything! She also took care of our orders and offered council in any situation. Her long experience in the lab and work with the iGEM teams of the last years enabled her to help with very specific problems and the handling of any machine. And even when she was busy she always took time for us and never let us down!

Maximilian Anders

max Max is amazing! He knows (according to everyone else) the most unconventional methods to try that always work. He was a great help with mutagenesis primer design, back-to-back PCR and ligation.

Robert Rauscher

robert Robert is master of the cell culture and keeper of the lung epithelial cells that he gave us for our toxicity tests and the lung-on-a-chip device. He also gave us advice whenever needed.

Christine Polte

Christine is the second technical assistant in Prof. Ignatova’s lab. She ordered lab material for us and made sure we got everything.

Katrin Kröger

Katrin is the secretary of Prof. Ignatova and helped us with the paper work and legal questions. Without her we would never have made it.
  • Suki Albers
  • Giovanni Bampi
  • Kaya Bittkau
  • Irina Chelysheva
  • Johannes Kaub
  • Andriy Kazantsev
  • Thorsten Mix
  • Priyanka Nair
  • Johannes Wagner
  • And all associated

Heisig Lab

Heisigs

Gudrun Melles

Gudrun Gudrun is one of the technical assistants of the Prof. Heisig’s lab and was a great help during our time in the S2 lab. She knows where everything is and the best methods. Her advice was especially valuable for the toxicity tests and the work with Klebsiella pneumoniae and Pseudomonas aeruginosas. She gave us different strains and everything else we needed to work with them. Gudrun also showed us how to identify and classify bacterial strains. For many of us it was the first time in a S2 lab and she helped us to work our way through it to get comfortable. She tried different DNA measurement techniques with us to see if it makes a difference (not really, but it was cool to try!). Not to forget she supported us during our rushed relocation to the pharmacy after the closure of our chemistry department and run some gels for us while we did not yet have security clearance and couldn’t work.

Philipp Groth

DerGrothe Philipp the second (seriously, why is every man in this lab called Philipp?) is also a technical assistant and a very cool guy. He helped us during our move to the pharmacy and with any questions and help with machines. He always found solutions and lightened our mood when nothing seemed to work out the way we wanted to. In the last stage of our experiments he supervised the chemists during their kinetic measurements of the complexation without despairing.

Anna Podolska

Anna Anna worked on the same floor where our S2 lab was and our daily contact person in the Heisig lab. She explained to us how to use the machines, made us feel welcome in the lab and was always there for us when we needed help. She really is a sweetheart!
  • Phyllis Zimmermann
  • Alina Schwarz
  • Philipp Hebel
  • Greta Gulbins
  • And all associated
They all helped us after our sudden move from the chemistry department to the pharmacy department after the labs had to close and made us feel welcome and part of their group. They showed us around in the new lab, taught us how to handle the higher security degree and even shared their materials with us. Without their great support, we couldn’t continue working and would have been forced to give up the project or at least lost a great amount of time. Thank you very, very much for everything you did for us, your friendship and the cookies! And the cake! And all the coffee!

Advisors

Henning Jacobsen

henning Henning started his PhD on virology in November 2016. He was an iGEM-Teamleader in Hamburg in 2015 and now acts as advisor for the following teams. He is basicly our bad guy, constantly telling everyone that he or she is too lazy and that we need more money. If Henning attends the seminar to “make a serious announcement” he is feared more than Prof. Snape. Besides the fact that he is at least right about the last fact, he further developed the concept of our lab and is a driving force behind new non-scientific collaborations. He already did a great job advising the last team in 2016 (Finding Chlamydory) and agreed on serving another year as advisor for our team. Thank you for your support!

Daniel Wedemeyer

Daniel Daniel was one of the team leader of last year’s team and offered his support as advisor of our team. After receiving his O.W.L grades leading iGEM Team Hamburg 2016, Daniel is Prefect, uhm, advisor for this year’s team. With Hamburg 2016 he was on hunt for Chlamydories, a completely different kind of creatures they were only able to catch with their magic detection bacteria. As advisor, he introduced us to BioBrick design, Defence against the Dark arts, HTML and the wiki, sponsor acquisition, and is always happy to share his experience in Niffler taming. Thank you for your advice!

Former Members

Katharina Hoffmann

She helped us a great deal at the beginning of our project but sadly couldn’t stay since she moved for a semester abroad to South Korea. Thank you for being part of our team!

Dorian Orlikowski

He helped us with sponsoring and later in the lab too, but went on to see the world too. Thanks for your help!

Caroline Oertzen

She was involved in the sponsoring but had to put her regular studies first. Thanks for being with us!

Supporting Cast

  • Christian Stark → he offered his lab for the chemical synthesis of the siderophores and supervised the experiments.
  • Martin Trebbin → he and his group provided the lab for the nanoscience experiments and supported the project.
  • Joanna Fafinska → always gave advice and helped us obtaining chemicals.
  • Thorsten Mix → provided us with betaine that we needed for our cooperation with the iGEM Team Heidelberg.
  • Nediljko Budisa → he gave us a lecture about synthetic biology and introduced us to the field.
  • Kai Kiehn → he helped us plan the future of our iGEM team and organised the sponsoring by the Alumni Verein.
  • Mirko Himmel → he held a lecture about biological safety and dual use research of concern, his counselling was valuable for the safety-related assessment of our project. Thank you for your input and taking the time answering all our questions about biosafety!
  • Rolf Müller → Thank you for the lecture about the development of new antibiotics and especially the tip about the integration of an synthetase-activating enzyme to get a viable amount of active enzyme. Thanks to him we added sfp to our gene collection.
  • Daniel Wilson → he held a talk at the public lecture round about common antibiotic targets and talked to us about general concepts of antibiotic development.
  • Wolfgang Streit → he gave us advice about our target organism Pseudomonas aeruginosa and offered us to use his lab.
  • Holger Rohde → he introduced us to novel data about multi-resistant bacteria in Hamburg and worldwide and how to acquire useful testing strains.
  • Peter Hauffler → for his support at the creation of a business plan.
  • Jochen Brinkmann → for helping us work out a business plan.
  • Ramakrishna Vasireddi → phD student in the lab of Prof. Trebbin who helped Nico at the development of the lung-on-a-chip device and showed him the lab.
  • Felix Rogowitz → he works for Fluigent and helped us to install and use the pump system they lend us for our experiments.
  • Lecture Series about "Antibiotic Resistances" → thank you to all the lecturers (Prof. Dr. Peter Heisig, Prof. Dr. Wolfgang Streit, Prof. Dr. Holger Rohde, Dr. Anselm Lehmacher, Prof. Dr. Stefan Schwarz, Dr. Sudanne Huggett, Prof. Dr. Andrew Torda, Dr. Anke Heisig, Prof. Dr. Zoya Ignatova, Prof. Dr. Stefan Niemann, Prof. Dr. Daniel Wilson, Prof. Dr. Dr. Christian Betzel, Prof. Dr. Henning Tidow and Prof. Dr. Markus Fischer), who gave us valuable insight about the development, dispersal, analysis and consequences of antibiotic resistances in Germany as well as in the USA and the rest of the world. Thank you for your patience in answering our questions and giving us advice about our project. Here we met Prof. Heisig for the first time who later generously offered one of his group’s labs for us to perform our S2 experiments and supported us during the entire time.
  • Miriam Block → for her support at the university and the marketing of our events such as the Dies Academicus. She makes sure that future iGEM teams get more support from both the university and the city of Hamburg.
  • Mirjam Brassler → for her cool seminar about interdisciplinary learning and the get-together at the barbecue later. Also thank you for doing our job and writing the article yourself!
  • FSR Lehramt Uni Hamburg → for the support and organising of the Human Practices Event for the education majors!
  • Lars Rohrssen → for his helping us with the Wiki.
  • Nina Kähler → for proofreading our texts and taking our DNA to the post.
  • Martin Äpfelbacher → for his expert knowledge on yersiniabactin which helped us a great deal during our safety assessment.
  • Gunnar Ehrlich → he provided the chemists with chemicals and helped out in many other ways.
  • Thomas Hackl → offered the chemists to measure a gallium-NMR spectrum (not featured in the wiki).
  • The chemists also would like to thank the following for either chemicals, advice or something else: Mauricio Coderch, Lilia Marcinkiewicz, Kathrin Hoppe, André Behnk and Philipp Merkel.
  • Our Collaboration Partners

    TU Delft

    The TU Delft organized this year’s European iGEM Meet up where a delegation of our team had a great time participating! We met many new friends and cooperation partners for our project. The topic of the TU Delft iGEM team fit very well to ours and we considered a cooperation which sadly did not work out in the end due to lack of time. Another cooperation with them and the iGEM Team Aalto-Helsinki couldn’t be realised for the same reason. Thank you for the year and the cooperation offer!

    Evry

    Evry is a great team with lovely people and great scientists! We had trouble with a ligation of a gen in three parts, and they found the solution – a Golden Gate Assembly! Since we hadn’t designed one yet and were already delayed in our planning, we couldn’t work it out on our own. Jeremy developed a complete program for us ready to work with, from the primer design to sending us a suitable vector. They supported us during the entire length of the experiment. A great thank you to Jeremy and his wonderful Team Evry!

    Aalto-Helsinki

    We met the iGEM Team Aalto-Helsinki at the European iGEM Meeting in Delft. They developed a usage for the peptide dermcidine, but since they did not have access to a S2 lab they could not perform any tests. We offered them our facilities in cooperation with the TU Delft, unfortunately we did not have enough time for it in the end. Thank you for the nice time!

    Heidelberg

    The team Heidelberg is an expert in PCR an offered their help with their PCR-First Aid Service, which we used when we couldn’t get our reactions to work. Heidelberg listened to our problems and gave us valuable advice and tricks how to change our experiments. We went through several rounds of trial and error and they always came up with new ideas. Thank you to the team Heidelberg and especially Pauline for her immediate help!

    Harvard

    The iGEM Team Harvard developed a questionnaire to standardise interviews with experts. Since we had several interviews in the scope of our project, we were happy to participate working out some questions.

    KU Leuven

    The team of KU Leuven launched a wonderful program where the teams translate other projects into their own language. We took part in that project and thank the KU Leuven for their great idea and the teams of Duesseldorf-Cologne, Heidelberg, Pasteur_Paris, Peshawar, IONIS, Moscow_RF, NYMU_Taipei, Tec Chihuahua, UChile_OpenBio_CeBiB und XMU-China for their translations!

    Peshawar

    We had a Skype call with the iGEM Team Peshawar to exchange our experiences. Thanks for the nice meeting!

    Cologne-Duesseldorf

    As last year, the iGEM Team Cologne-Duesseldorf started a postcard information campaign in which we gladly participated. We designed a postcard ourselves and were send some by other teams, which we distributed at our university and events.

    Franconia (Erlangen & Würzburg)

    We could help the Team Franconia by providing some space on our poster at the Giant Jamboree to be able to perform the game they planned for the event: all the participants of the Jamboree together must look for a cure for an epidemic! What they don’t know: where on the side of the enemy! Thank you, Franconia, for letting us participate in your game!

    Waterloo, UNebraska, Dalhousie, Greece, Vilnius-Lithuania and Washington

    All of them created a survey that we participated in. We hope, it helped these teams to find their answers!

    Our Sponsors

    Universität Hamburg

    “Universität Hamburg is the largest institution for research and education in the north of Germany. As one of the country's largest universities, it offers a diverse course spectrum and excellent research opportunities.The University boasts numerous interdisciplinary projects in a broad range of subjects and an extensive partner network with leading institutions on a regional, national and international scale.”

    We thank the University of Hamburg for making our journey to Boston possible.

    Claussen-Simon-Foundation

    “The Claussen-Simon Foundation supports talented young individuals throughout their academic, professional, and personal development by offering educational oppurtunities and funding.”

    The Claussen-Simon-Foundation supports the iGEM team of the University of Hamburg for three years in order to establish it and to facilitate student research. They did not only offer generous financial aid but also ideally support.

    Alumni Verein Hamburg

    “The purpose of our Association is to promote research, teaching and education. Our driving force is our enthusiasm for our cause. We want to promote students, connect alumni and promote international exchange. We want to build bridges between theoretical research and practice as well as between Hamburg and the world.”

    The Alumni Association helped us throughout our entire project and give us new perspectives for the following iGEM Teams in Hamburg. We thank the Alumni Association and thereby every member of it for supporting our project.

    B. Braun

    “B. Braun is one of the world's leading providers and manufacturers of healthcare solutions today. Every service that B. Braun provides incorporates the entirety of our knowledge and skills, the company's deep understanding of users' needs, and extensive expertise since 1839.”

    B. Braun gave us financial aid to enable our travel to Boston.

    Jung - Stiftung für Wissenschaft und Forschung

    „The Jung Foundation promotes basic research in human medicine and especially the further research of clinical importance which builds upon it and which can contribute to the future of treatment. […] The Jung Foundation's activities are committed to the ideal of human medical science and healing arts. They serve to continuously improve the state of medical knowledge, treatment options and patient comfort.”

    Thank you for your financial aid that made the travel to Boston possible!

    Carl Roth

    “Carl Roth, the expert consultation for labware, life science and chemicals for more than 138 years. […] Furthermore, we develop our own new, future-oriented ROTH products based on research projects and cooperations. In this way, we establish a foundation for the success of today's and tomorrow's research and analysis.”

    Thank you for rendering our travel to Boston possible!

    SnapGene

    “SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures. Cloning is simpler when you can see exactly what you are doing. SnapGene is the first molecular biology software that is easier to use than pen and paper.”

    Snapgene gave us the opportunity to easily manage our cloning strategy and helped us through many problems by providing a robust and simple to use software. We thank SnapGene for their support!

    Eppendorf

    “Eppendorf is a leading life science company that develops and sells instruments, consumables, and services for liquid-, sample-, and cell handling in laboratories worldwide.”

    As part of our Human Practices Project Tag des Wissens / Day of Knowledge, Eppendorf provided team hoodies for our team. We are thankful for the support!

    IDT

    “Integrated DNA Technologies, Inc. (IDT), the global leader in nucleic acid synthesis, serving all areas of life sciences research and development, offers products for a broad range of genomics applications. IDT’s primary business is the production of custom, synthetic nucleic acids for molecular biology applications, including qPCR, next generation sequencing, synthetic biology, and functional genomics.”

    IDT synthesised 20.000 bp for us so we could get our project started!

    NEB

    “Founded in the mid-1970s as a collective of scientists committed to developing innovative products for the life sciences industry, New England Biolabs is now a recognized world leader in the discovery, development and commercialization of recombinant and native enzymes for genomic research.”

    NEB sponsored us with the BioBrick-Assembly Kit and some other products.

    GATC

    “GATC Biotech, a family-owned business since 1990, is Europe’s leading sequencing services provider with more than 10,000 customers from academia, healthcare and industry worldwide. The company consistently contributes to the sequencing field with innovative products, scientific expertise and rigorous quality. “

    GATC sponsored us with sequencing of our products.

    Fluigent

    “We make smart microfluidics - We develop, manufacture and support the most advanced microfluidic systems available. Whether your application is with droplets, cell biology, particle studies, or in other research areas, we have the expertise and knowledge to provide the most cost effective and technically advanced solutions to your fluid control needs.”

    Fluigent is the producer of a special pump systems that we needed to carry out the experiments with our microfluidic lung-on-a-chip device. Thank you very much for providing us with your system!