Revision as of 14:41, 1 November 2017

Results

Built a hybrid promoter that implements AND-gate logic evaluation of L-lactate and AHL and leads to expression of a gene under its control.
Tuned the AND-gate such that it is capable of distinguishing lactate levels associated with healthy and tumor tissue as well as taking into account the bacterial population density.

Characterized and tuned expression of a genetically encoded MRI contrast agent bacterioferritin in E. coli Nissle 1917.
Showed that the contrast agent indeed leads to a marked decrease in the MRI signal which demonstrates its usability as an MRI contrast agent in vitro and confirms the potential to use it as an in vivo reporter of tumor sensing.

Developed an assay to characterize killing of cells in a cell line when supplemented with supernatant of lysed bacteria expressing azurin.

Built and characterized a thermoresponsive system that induces expression of a controlled gene at 45 °C but not at 37 °C.
Built an RBS library for the Heat Sensor, whereby we reduced leakiness far enough to transform the potent lysis-inducing protein E under its control.

Showed that we can induce lysis of the bacteria when transformed with protein E under the thermosensitive promoter and induced at 45° C.

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      <h1>References</h1>
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          <li id="bib1">“Open source Focused Ultrasound system now available to researchers around the world.” <cite>Focused Ultrasound Foundation</cite>, 24 May 2016. <a href="https://www.fusfoundation.org/news/1762-open-source-focused-ultrasound-system-now-available-to-researchers-around-the-world">URL</a></li>