Difference between revisions of "Team:ETH Zurich/Composite Part"
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| + | <p><em>Here you can read details about our Composite Parts. To get an overview of all parts we created, visit <a href="https://2017.igem.org/Team:ETH_Zurich/Parts">Parts.</a></em></p> | ||
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Revision as of 16:07, 1 November 2017
Composite Parts
Here you can read details about our Composite Parts. To get an overview of all parts we created, visit Parts.
Overview
All parts were codon-optimized for expression in E. coli Nissle with Geneious and modified to remove forbidden restriction sites.
| Part | Description | BioBrick |
|---|---|---|
| pConstRBSeng_TlpA | Best Composite Part: Constitutively expressed temperature-dependent transcriptional repressor of pTlpa with engineered RBS | BBa_K2500008 |
Design
BBa_K2500008: pConst_RBSeng_TlpA
Design notes: A RBS library was created using the Red Libs algorithm to find variants translating more TlpA RNA in order to create a thermoswitch tight enough to repress the premature induction of cell lysis by protein E.
Organism: Salmonella typhimurium
Based on: BBa_J23104 (promoter) and Piraner, Dan I., et al. Nature Chemical Biology 13.1 (2017): 75-80. (TlpA)
Source: FIXME RedLibs + gBlock
Registry: BBa_K2500008
