Overview 1. Conducted Simulation in order to simulate filter’s working ability and improve our design. 2. Used the equation of Michaelis-Menten kinetics model to test our HRP degradation efficiency. 3. Derived Freundlich Adsorption Isotherm model to measure the adsorption capacity of our filter. 4. Modeled the design life of our filter to predict the frequency of replacement of inner activated carbons.

Model 1: Enzyme kinetics

Theory and derivation of enzyme kinetics :

We simplified our enzyme-EDCs system as the reaction with the process of the first and second order reactions:

The initial collision of E (Enzyme) and S (Substrate) is a bimolecular reaction with the second-order rate constant k₁. The ES complex can then undergo one of two possible reactions: k₂ is the first-order rate constant for the conversion of ES to E and P, and k_-1 is the first-order rate constant for the conversion of ES back to E and S.We assume that the formation of product from ES (the step described by k₂) does not occur in reverse.

The rate equation for product formation is:

However, measuring [ES] is more difficult because the concentration of the enzyme-substrate complex depends on its rate of formation from E and S and its rate of decomposition to E + S and E + P:

To simplify our analysis, we choose experimental conditions such that the substrate concentration is much greater than the enzyme concentration and ES remains constant until nearly all the substrate has been converted to product.

According to the steady-state assumption, the rate of ES formation must therefore balance the rate of ES consumption:

The total enzyme concentration, [E]T, is usually known:

This expression for [E] can be substituted into the rate equation to give:

Rearranging (by dividing both sides by [ES] and k1) gives an expression in which all three rate constants are together:

We defined KM and rearranged the equation:

We did some compute process for the equation and give:

Solving for [ES],

Finally, we can express the reaction velocity as:

The maximum reaction velocity, Vmax, can be expressed as:

And then we can get the equation,

In order to estimate the degradation capacity of the filter, we calculated horseradish peroxidase’s ability of degradation based on the equation of Michaelis-Menten kinetics :

The degradation rate under different EDCs concentration

We used several parameters according to the paper we found to calculate the initial velocity of different concentrations of BPA and NP. Also, their concentrations at different time after starting degradation.

The results are shown in the following figures(Figure 1-4) :

Figure 1. degradation speed vs EDCs concentration under the maxmium EDCs concentration of 10^(-6) mg/L

Figure 2. degradation speed vs EDCs concentration under the maxmium EDCs concentration of 10^(-7) mg/L

Figure 3. degradation speed vs EDCs concentration under the maxmium EDCs concentration of 10^(-8) mg/L

Figure 4. degradation speed vs EDCs concentration under the maxmium EDCs concentration of 10^(-9) mg/L

From the results of the enzyme kinetics, we can find that when the concentration of EDCs increases, the degradation rate increases dramatically. As a result, our activated carbon in the system of our filter can help HRP to degrade EDCs in the more efficient way since its outstanding ability to capture EDCs in the water and accumulate more EDCs around HRP.

To prove the accumulative ability of activated carbon

To prove that our activated carbon can effectively accumulate our EDCs, we compared the time duration for 50%,75%, and 95% degradation between different concentrations of EDCs in the unsaturated activated carbon and those in the saturated activated carbon. (Since we believed that if our activated carbon can effectively accumulate EDCs, the EDCs solution should we saturated)

We fixed the EDCs solution at the concentrations of 10^(-6) M,10^(-7) M,10^(-8) M, and 10^(-9) M. (not in the activated carbon)The results are shown in the following figures(Figure 5-19) :

Figure 5. time duration for 50%degradation vs EDCs concentration under the unsaturated activated carbon with the concentration of 10^(-6) M

Figure 6. time duration for 50%degradation vs EDCs concentration under the unsaturated activated carbon with the concentration of 10^(-7) M

Figure 7. time duration for 50%degradation vs EDCs concentration under the unsaturated activated carbon with the concentration of 10^(-8) M

Figure 8. time duration for 50%degradation vs EDCs concentration under the unsaturated activated carbon with the concentration of 10^(-9) M

Figure 9. time duration for 50%degradation vs EDCs concentration under the saturated activated carbon with the concentration of 10^(-6) M

Figure 10. time duration for 75%degradation vs EDCs concentration under the unsaturated activated carbon with the concentration of 10^(-6) M

Figure 11. time duration for 75%degradation vs EDCs concentration under the unsaturated activated carbon with the concentration of 10^(-7) M

Figure 12. time duration for 75%degradation vs EDCs concentration under the unsaturated activated carbon with the concentration of 10^(-8) M

Figure 13. time duration for 75%degradation vs EDCs concentration under the unsaturated activated carbon with the concentration of 10^(-9) M

Figure 14. time duration for 75%degradation vs EDCs concentration under the saturated activated carbon with the concentration of 10^(-6) M

Figure 15. time duration for 95%degradation vs EDCs concentration under the unsaturated activated carbon with the concentration of 10^(-6) M

Figure 16. time duration for 95%degradation vs EDCs concentration under the unsaturated activated carbon with the concentration of 10^(-7) M

Figure 17. time duration for 95%degradation vs EDCs concentration under the unsaturated activated carbon with the concentration of 10^(-8) M

Figure 18. time duration for 95%degradation vs EDCs concentration under the unsaturated activated carbon with the concentration of 10^(-9) M

Figure 19. time duration for 95%degradation vs EDCs concentration under the saturated activated carbon with the concentration of 10^(-6) M

As we can see in the results, the scale of time duration for degrading the unsaturated EDCs solution can up to 10^(15) sec, however, the time duration for degrading the saturated EDCs solution are only 10^(10)sec. This proves that our filter can effectively accumulate EDCs to let our enzyme degrade much more faster.

Model 2: Concentration Test

Theory and derivation of the Freundlich Adsorption Isotherm from Kinetics

In general, the rate law can be written as:

If we imagine a small pore, adsorption sites near the open ends are different from at the center of the pore. This distinction occurs when the sites possess specific chemical properties. If a concentration is imposed at one end of the pore, solute must diffuse to find an open adsorption site first and it is similar as the desorption case.In this point, the exponent n is a measure of the fractal dimension of the process and the rate constant for fractal reactions is also expected to be proportional to the molecular diffusion coefficient.For both adsorption and desorption reactions but with distinct fractal dimensions (n_i) and fractal rate constants (k_i):

At equilibrium, the derivative is equal to zero and the equation can then be solved for S:

Finally, we can transform the equation to the Freundlich Adsorption equation,

C_e : the equilibrium concentration of the solute in the bulk solution (mg L−1)(the concentration of EDCs)
K_F : Freundlich constant indicative of the relative adsorption capacity of the adsorbent (mg1−(1/n) L1/n g−1)
n : Freundlich constant indicative of the intensity of the adsorption
q_e: the amount of solute adsorbed per unit weight of adsorbent at equilibrium (mg g−1)(the maximum of the adsorption)

To further estimate our filter’s function, we’ve conducted two models based on the Freundlich Adsorption equation as following:

The adsorption capacity under different EDCs concentration

To test the adsorption capacity under different maximum EDCs concentration, we than fixed our maximum EDCs concentration as Ce at 10^(-6) M, 10^(-7) M, 10^(-8) M,and 10^(-9) M, which represent 2.2*10^(-1) mg/L, 2.2*10^(-2) mg/L, 2.2*10^(-3) mg/L, and 2.2*10^(-4) mg/L respectively. The results are shown in the following figures(Figure 20-23) :

Figure 20. q_e vs C_e under the maxmium EDCs concentration of 2.2*10^(-1) mg/L

Figure 21. q_e vs C_e under the maxmium EDCs concentration of 2.2*10^(-2) mg/L

Figure 22. q_e vs C_e under the maxmium EDCs concentration of 2.2*10^(-3) mg/L

Figure 23. q_e vs C_e under the maxmium EDCs concentration of 2.2*10^(-4) mg/L

We can see that as the concentration of EDCs (C_e) increases, the adsorption amount per unit weight of adsorbent at eq. also increases. We deduced that it might because as the concentration arises, the collision frequency between EDCs and our activated carbon increases, leading to the higher amount of adsorbent.

The design life of filter under different EDCs concentration

To test The design life of filter under different maximum EDCs concentration, we first fixed our amount of activated carbon at 1000g and also fixed our maximum EDCs concentration at 10^(-6) M, 10^(-7) M, 10^(-8) M,and 10^(-9) M, which represent 2.2*10^(-1) mg/L, 2.2*10^(-2) mg/L, 2.2*10^(-3) mg/L, and 2.2*10^(-4) mg/L respectively. Based on the reference we found, we then assumed that the flow rate of water is 1.5L/sec and farmers will irrigate their farmland at the frequency of 7 days.Thus the total flow for each irrigation time will be 86400(sec)*7*1.5(L/sec)=907200(L).The results are shown in the following figures(Figure 24-27) :

Figure 24. The design life vs EDCs concentration under the maxmium EDCs concentration of 2.2*10^(-1) mg/L

Figure 25. The design life vs EDCs concentration under the maxmium EDCs concentration of 2.2*10^(-2) mg/L

Figure 26. The design life vs EDCs concentration under the maxmium EDCs concentration of 2.2*10^(-3) mg/L

Figure 27. The design life vs EDCs concentration under the maxmium EDCs concentration of 2.2*10^(-4) mg/L

We can see that as the concentration of EDCs increases, The design life of filter decreases. It may because the more EDCs are presented in the water, the more activated carbon will bind to it, which then reduces the amount of activated carbon we can utilize and it proves that our filter can eliminate the EDCs in the lower concentration but it can’t afford the concentration of EDCs beyond the safe range.

Enzyme Kinetics

Freundlich Model

Reference

1. Dong, S., Mao, L., Luo, S., Zhou, L., Feng, Y., & Gao, S. (2014). Comparison of lignin peroxidase and horseradish peroxidase for catalyzing the removal of nonylphenol from the water. Environmental Science and Pollution Research, 21(3), 2358-2366.

2. http://www.hccfa.org.tw/

3. Hamdaoui, O., & Naffrechoux, E. (2007). Modeling of adsorption isotherms of phenol and chlorophenols onto granular activated carbon: Part I. Two-parameter models and equations allowing determination of thermodynamic parameters. Journal of Hazardous materials, 147(1), 381-394.

4. Skopp, J. (2009). Derivation of the Freundlich adsorption isotherm from kinetics. J. Chem. Educ, 86(11), 1341.

5. Choi, K. J., Kim, S. G., Kim, C. W., & Kim, S. H. (2005). Effects of activated carbon types and service life on the removal of endocrine disrupting chemicals: amitrole, nonylphenol, and bisphenol-A. Chemosphere, 58(11), 1535-1545.