Team:NTHU Taiwan/Contribution

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Contribution

Interlab measurement study

We have finished the interlab experiments and get lots of results in the page of interlab.




Contribution of new parts

We have contributed 12 new biobricks for iGEM part registry and submitted 9 parts to iGEM headquarter.(Table 1)


　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　
biobricktype name description designer length
K2354000basic HRP Degrade Estrogen Disrupt Chemicals Chan, Wei-Jen 927
K2354001basic Laccase BP Degrade Estrogen Disrupt Chemicals Chan, Wei-Jen 1572
K2354002basic Laccase SC Degrade Estrogen Disrupt Chemicals Chan, Wei-Jen 1539
K2354003basic Laccase TC Degrade Estrogen Disrupt Chemicals Chan, Wei-Jen 1593
K2354004basic Laccase TV Degrade Estrogen Disrupt Chemicals Chan, Wei-Jen 1557
K2354005basic monobody TV Bind to ER alpha when ER alpha is bind with EDCs Chan, Wei-Jen 435
K2354006basic MNP TV Degrade Estrogen Disrupt Chemicals Chan, Wei-Jen 1095
K2354007basic ER-alpha TV Bind to EDCs Chan, Wei-Jen 759
K2354008basic monobody Bind to ER alpha when ER alpha is bind with EDCs Chan, Wei-Jen 288
K2354009basic ER-alpha HS Bind to EDCs Chan, Wei-Jen 897
K2354010composite monobody-GBP HS Bind to ER alpha when ER alpha is bind with EDCs and it can bind on the gold surface. Chan, Wei-Jen 797
K2354011composite INP-ER-alpha HS Expression of ER-alpha on the membrane of E. coli and ER-alpha can bind to EDCs Chan, Wei-Jen 2199
K2354012composite HRP-his tag Degrade Estrogen Disrupt Chemicals Chan, Wei-Jen 1217

Table 1. the list of our 12 new parts




Validation of composite parts’ function

We constructed three composite parts and all of their function have been validated:

(1) Part:BBa_K2354010 :monobody-GBP HS

Monobody was fused with gold binding protein and linked with 6×his tag. This fused protein can bind to the gold surface by the function of the gold-binding protein, and it can thus capture our ER-alpha/EDCs complex.



(2) Part:BBa_K2354011 :INP-ER-alpha HS

ER-alpha was fused with ice nucleating protein. This fused protein can be anchored to the membrane of E. coli via the function of ice nucleating protein and can bind to EDCs for detection via ER-alpha.



(3) Part:BBa_K2354012 :HRP-his tag

Horseradish peroxidase is a kind of peroxidase which uses heme as a cofactor, and it can degrade EDCs to the less toxic compounds. Moreover, we linked fused protein with 6×his tag for purification.