Difference between revisions of "Team:RHIT/Safety"

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<h1> Safety </h1>
 
<h1> Safety </h1>
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<p>Please visit <a href="https://2017.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>The synthetic biology and tissues laboratory at Rose-Hulman Institute of Technology was where all experimentation and handling of any organisms took place over the course of 2017 iGEM season. These labs were set up as enhanced biosafety level one labs.<br>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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All organisms and parts used were biosafety one level certified according the the NIH biosafety guidelines. All new parts were created synthetically using Integrated DNA Technologies’ synthesizing services.<br>
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</div>
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<b>Organisms handled:</b>
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<br>
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</p>
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<div class="column full_size">
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<h5>Safe Project Design</h5>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<ul>
 
<ul>
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<li>Choosing a non-pathogenic chassis</li>
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<li>E. coli strains</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>NEB DH5α Competent Cells</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>MG1655 (DH3) - Prather Strain #: 663</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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</ul>
 
</ul>
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<p>
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<br>
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The DH3 mutation integrates Lambda DE3 into the chromosome. This mutation encodes T7 RNA polymerase. This specific mutation was not relevant to the project, and was used as a substitution for the parent strain MG1655<br><br>
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<b>Organisms from which parts were derived:</b>
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</p>
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<ul>
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<li>Lactobacillus casei</li>
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<li>Bacillus subtilis</li>
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<li>Streptococcus mutans*</li>
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<li>Thermoanaerobacter tengcongensis</li>
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<li>Escherichia coli K-12 substrain MG1655</li>
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</ul>
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<p>
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<br>
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<i style="text-indent: 5em">*Note that the form of Streptococcus bacteria used- S. mutans- is a biosafety one organism  in the NIH database -unlike its relatives.</i><br>
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<br>
  
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</div>
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Uncommon reagents used were the Leucovorin Calcium (tetrahydrofolate calcium salt) and Cyanocobalamin. MSDS pages were consulted, and these reagents were solely handled in the chemical hood before being placed into solutions. Traditional personal protective equipment were used at all times during the handling of these two reagents. Extra care was taken to avoid inhalation of Leucovorin Calcium when not solvated.</p>
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<div class="column half_size">
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<h5>Safe Lab Work</h5>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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</div>
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<div class="column half_size">
 
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<h5>Safe Shipment</h5>
 
  
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
 
</div>
 
</div>
  
  
 
</html>
 
</html>

Latest revision as of 17:37, 1 November 2017

HOME
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SAFETY
HUMAN PRACTICES
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Model
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JUDGING FORM

Safety

The synthetic biology and tissues laboratory at Rose-Hulman Institute of Technology was where all experimentation and handling of any organisms took place over the course of 2017 iGEM season. These labs were set up as enhanced biosafety level one labs.
All organisms and parts used were biosafety one level certified according the the NIH biosafety guidelines. All new parts were created synthetically using Integrated DNA Technologies’ synthesizing services.
Organisms handled:

  • E. coli strains
  • NEB DH5α Competent Cells
  • MG1655 (DH3) - Prather Strain #: 663


The DH3 mutation integrates Lambda DE3 into the chromosome. This mutation encodes T7 RNA polymerase. This specific mutation was not relevant to the project, and was used as a substitution for the parent strain MG1655

Organisms from which parts were derived:

  • Lactobacillus casei
  • Bacillus subtilis
  • Streptococcus mutans*
  • Thermoanaerobacter tengcongensis
  • Escherichia coli K-12 substrain MG1655


*Note that the form of Streptococcus bacteria used- S. mutans- is a biosafety one organism in the NIH database -unlike its relatives.

Uncommon reagents used were the Leucovorin Calcium (tetrahydrofolate calcium salt) and Cyanocobalamin. MSDS pages were consulted, and these reagents were solely handled in the chemical hood before being placed into solutions. Traditional personal protective equipment were used at all times during the handling of these two reagents. Extra care was taken to avoid inhalation of Leucovorin Calcium when not solvated.